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(1991) e3de;@e33z>> eseo c d j © QjeV3 G^zs* Ogzsf e6)3C025* Sgzsteea'za Sd c#co3®. 25*©e#23 ep°23 : 2A. ©325*23 ©dscsaS® £>2s*23, d^zsgdcs. (1994) ~ eseo eo^ecoiezsfS £>25*©2s* Sd c&&& 66)3025* ef3-2s*@e*23 Sd e#coc3®. 2s*@e3°23 ep°23 : 2A. ©325*23 ©d3z8© 025*23, dzrfzngdca. (1994) ©Ozs* ©025*0 ©zasOedOe SQOs c#2a*, 333. geoOc325*©co25* ecbcozj* Sdcaoza esd©od30e qdQizsizi 1 Oq)@025* ^ 25*03 c#23. 1 OcoO: Sdcapza e3d©£3d30e epedOt25*23 (Pes/ ©eozaV zS© ©25* zS.co )^ ®D ©3Z3c3 epedO^ 23*23 K 2023 (Seci-zsfseiza) 2701 K DG 39 (Sa)-Z5*@eiza) 1706 K2016(a?|-afe@te>) 1590 2025 X DN 1517 K2021 (S)g-afo@*&>) 1470 2025 X DN 1412 K DN (Seci-zsJ'eeiza) 1376 K 2025 ( a g - H f e @ t e > ) 1253 etf .@Z5*.6© .d&seedzs ,@es*.e53 .625*.8c3e3]25*5d,6® .dssfeaacsza, 6es\e)S. CQ-so^ aes eseo 3.a»23*gtod3d3. 2.1.9. VP 74 - o"9@D edO?S Sd G^eztf <3-25*©e*23 Sd (DN X 2025) eseo eagSdrarft; Sd QCXSZSS 6)03 Z5J©ei25> Sd c#C0c3®. ZsS&iG) 3«23 14, ©325*23 23©Sed 025*23), 2360325)J©6'. 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Sszaza'Sed zsteeizacazsfiscd eadoco ea®«o © g^egzrf zsdza e$ zsj. eajeeaza'zaO) egefea o®5za$, eoOcozrf £>Q o@Sza$ oeoza qzsOs ep^ za. 23J. e3@£325&DD3 6© 92ScS2at3 TRI 777 48 0 DT1 29 10.3 HS 11 OA ( § - < 3 ^ CB3Z3C3Z5?) 160 7.5 TRI 2025 32 15.6 zsj. e3i©eaza^ s»e)3 ©szsca 823a ©szsca ©eea ©coza HS 10A zstee:fza<3c3* g d o 230 ea@CD zsdza Q$ eadsrozaeca? d e eoO rojzS® 0.86% zsfSca. e30 - eadscozaca ea®eod dza§ca zatee^ feaOe e3d©e3d30za° e^cotzS® ea«jeo3 gasb ®g z§e eaOodscozaca 3 ® 0 63jei63*g zagzsj* et)<3 e&3C02a ©zaseofzSSca. edQ eadscozaca 3 ® 0 eaz&edg zateeJza eaeo agedd ogfiza oeoza ^zsOs epjza: 2ste(3izaca g d d oeSza TRI 2024 103 TRI 2025 10 TRI 777 50 TRI 2043 50 DT95 75 TRI 2142 10 ASM 4/10 20 tied. o ®5 , S.&d2sfea36a>3D, eseo O.sszrigcedsds. 4. sstee^za o d ® o d 3 odzsfess zSd® : (©eg epOSca) 4.1. O C 0 2 3 dO 4.1.1. LVP 55 (LVP 30/LC/l) dted&sa eaeo figsoadfta® OzaOgza* ©0)3023* Sd ®&sf eabcozj* zsfees'zacaza'^coa®. (LVP 30 a -^ fc rc (1983) rata©**© Ojsfta, s^ zScasca. (1991) @$Oza p^OSca caOszrf 5.2.3. Sezrfza. 65 4.1.2. LVP 56 (VP37LC/ 1) - VP 37 (©323*23 23®a)e3*) ®<3z3* e«>3C02s* 23°©e3*23G3Z3* e#eo3©. ^©esfg ewa 5, 033Z3*z3 ©dscazS© Ozste), dzsfzngdca. (1992) 4.1.3. LVP 57 (LVP42/LC/1) code® Sstarf OO30O2s* Sg-23*©e3*z3 Sd ©323 ®<3Z3* (36)30023* 25f©ei23 e£co3®. (LVP 42) 2steas*23 ewa 5, ©ozsfea ©docazS© 023*23, dzsfzogdca. (1992) 4.1.4. LVP 58 ( L W 28/LC/l) - tfes^eS 023^3©^^ (LVP 28 09323*3) ©d3c32S© 023*23 1983) 253*©£3*23 £5=23 5, 03323*23 @d3c32§® 023*23, d23*23gdc3. (1992) 4.1.5. LVP 59 - ©£303(3)6 Ozstea* ©Osozs* §*3- aateeizs (2026 X DN) Sd 03323 ®&7si eabeozs* zstees'zs €£0o8®. 23*©63*23 epo23 5, CSOZSte) ©d3c32§® 023*23, d23*23gdc3. (1992) LVP 56,57, 58 eaeo 59 ®<323* ©23^30023* eazsjszssOcaza* epjS 23*©(33°z3 ©§023 efOS©&3* ep23*eo§3 3z§®2s* C30©23* 0030023*233 (3§\ (©§023 €?03c3 c30©23* 5.2.4. 9(323*23) &.@23\d®.dc9@e3*23, ©d.63.d»23*. 8c9g23*§d, &S3*. £)«). 02«O§3e3, 6®. d23*233&323 eSCO S.fflZEifjCodsds 4.1.6 LVP 61 (LVP 42/ LC/1) essd©® Ozstezs* 0030023* 3g-2s*®e3*23 Sd 09323 ©6323* (30)30323* zsfeeizs €£oo3®. (LVP 42) 23*©as*23 ef.23 2A 63eo 5N, 09323*23 ©dscazS© 023*23, d23*z3gdc3. (1993) OjS® efC3*Oi23*2325)* ©S)3g23* 23*©(33*23 20 23 €5630^ 23*23 633<323c3 ©©63 @c33§3COZ3* TRI 2023 23*©(33*23©cd' 363*0^ 23*23 e3®C0 2 0CD©0Z3* §23*03 €£23. 2O0Op 23*©(33*230e €feJ0?23*23 ( 063/ ©6023*/ z8® ©23* zS.COl) 186 7514 160 6624 243 5951 253 5883 281 5864 285 5585 375 5464 342 5320 171 5226 168 5213 289 5080 298 5003 109 4996 119 4858 322 4786 10 4752 173 4614 169 4556 107 4481 66 4446 2023 £33(325X3 4437 cp.ee.g. (es3.= 0.05) 3030 SO. a.. % = 29.75 66 4.1.7. LVP62 (LVP 45 eseo LVP 46/LC/l) - 033253*253 ©dscsaS© Otstezsf Ooozae Sgzstee^a § d 0332a Ogzsf (LVP 45, 1989 eseo LVP 46, 1990)eS)3»25J 253*©6fe» 3*058®. zsteasfg 3.2a 2A, 5N, 033255*255 ©d3&32§® 0255*255, d255*25>gd - (1993) £336255 253*©©f253c3253* ©©63 00332556 TRI 2023 0 0£>3 (|)e06 363*023 Qattepi 25f®63*253 17 25* 3cS. 65)303255* 363*023 3 0255 Ocp©0253* $255*03 3*255. 3 0coO: 25X363^5306 363*0*253*253 ( Oes/ ©£0255*/ 253© ©255* zS.Otf) 25*©©fe5)G3 363*0*253*253 63 5516 11 4426 59 4349 47 4090 12 3855 2025( £33625)03) 3715 13 3692 64 3629 23 3624 49 3607 52 3565 54 3416 51 3294 55 3153 72 3037 36 2945 2027( £33623303) 2715 58 2703 10 2668 2023 (£3362S5&3) 2330 67 2169 70 2147 53 1934 30 1386 q.eQ.q. (ea®.= 0.05) 3053 S O . % = 40.37 4.1.8. LVP 63 - ©£303o36 OzsfeisJ Oroszae <3 - 25*®ete3 «3d 0332a (2026 x DN) Ogzss* 6^30325* zstee^za 3*033® £0e® 363*0*253*253 6^303255* 253*©63*255c3255*©o5 6360 €336253 253*@e3*255o3255*©Cr* 3630*255*255 4 0255 OcgsD $25503 3*253. 67 40q>0: zsfee^zaOe eeedOfgfea ( Sea/ ©eozsf/ zB© ©za* z8.c%) epedO^ za'za 19/12 10397 4/2 9106 21/23 8744 2023 (£33625X3) 6836 8/9 6293 30/7 6012 19/1 9626 1/2 5756 8/8 5654 2025 (£33(323X3) 5545 14/17 5477 29/22 5409 31/1 5409 11/11 5387 25/36 5193 23/16 5174 23/22 5173 15/18 5131 14/19 5057 2027 (£33625X3) 4883 c?.©0.es. (e3©.= 0.05) 3036 SO. tao. % = 32.45 4.1.9 LVP 64 (ASM.INT.88/LC/1)- 1988 eSscozrf ASM § d oie Ogzsf ©zaJdscoza' zsteeiza e^ cocS® zsteasfza qws) 2 A, ©szsfea ©dacszS© Ozsfza dzrtzagd - (1993) ^6 ©zag© Osd 33 23>? eSJozjf epdOizrfza ewgO zstegiza 8 zsf eseo £3362» Oe epedOzrj esEdOza OcgeOzsf ejzsfOs cftza. 5 OcgO: zsfee^zaOe epedOtzsfea ( Pes/ eeozsj/ zS© ©zsf zS.cpt) zsfee^zaca epedOfza'za 11 5074 5 4521 2023(£33e25)c3) 4313 10 4200 12 4193 2025(£33e25)c3) 3899 6 3228 19 2877 18 2533 16 2344 2027(e33e25xa) 1336 ep.eO.ep. ( e3®.= 0.05) 1049 3©. 63.. % = 9.91 68 4.1.10 LVP 66 (VP 39 / LC/1) - VP 39 p^sfeoep SjgsQzs* ©zsrfdscozs* 25*©(33°25> qiaxS© zsteefe) q°zs> 2 A, ©325*25) ©dscasS® 025*25) dtstzae^ d - (1994) eo<3 epedOzg ge; zsfeeiza 72a eseo &OQZS> TsteQJisxaisi&zi ^esOsg 6 Ozn OqxsOzi* ^25*03 ^2». 6 OcpO: 2sf®(33'25)e)e e^dOi2s*25) ( Oes/ ©eozs*/ 5§© ©25* 2§.cs#) 2af©e3°2acs ^esOjzsiza 2026(e3D(32ac3) 6478 2023( e33(325xs) 6016 113 5665 267 5115 95 4847 129 4665 33 4431 38 4292 222 4123 2027(e33(32s>cs) 4100 ep.®0.$. (<3©.= 0.05) 2786 S O . » . %=33.00 4.1.11 LVP 67 (VP 43 / LC/1) -VP 43 epzsfeoep Sjg©®25* ©zBidscozrf zsteeiza c^coS® 25*©d2S) q°zz 2 A, ©325*25) ©d3cS2§© 023*23) d23fc»gd -(1994) feoe epesOzg zsi®(3ii5) 31 2S> eseo 0302s 25*©e325>cS25*©<3* epesOz/j 7 Oza Ocg@025* epstQo qiis>. 7 OcpO: zafsedzaOe cjesO z^stea (Oe3/ ©£025*/ 23© ©23* zS.cpi) 25*©(3*23)C3 epedO?25*25) 814 7301 2023(£33e25)cS) 6003 710 5032 571 4539 759 4480 655 4440 708 4399 2025(£33(325)cs) 4367 679 4363 626 4254 757 4095 709 3996 554 3959 628 3943 840 3935 769 3932 685 3896 627 3828 69 621 3787 661 3749 612 3728 657 3706 624 3683 588 3624 770 3588 768 3520 504 3463 763 3434 557 3368 745 3348 702 3339 735 3262 744 3255 2027(e3De255C3) 3239 ep.eO.ep. (ea®.= 0.05) 4009 SO. <*..%= 46.31 4.1.12 LVP 68 (LVP 44 / LC/1) - VP 44 cpsfenep 3ig@®2sf saJdscDZji zateeiza efjcocS® ©zste^ ep°2a 2 A, ©szxfza ©dacazS© Etefca drfzagd - (1994) (gjcoe epedOzxj (35 zsteefza 1123 eaeo 03(323 2s*©eftnca25tecrf epedOz/j 8 Qza QqxsOzsf ejzsfOs eftza. 8QcoQ: zsteeizaOg epedOjzsj'za ( £>ea/ ©cozsf/ z53® eta" zS.cpj) zstee^zaca epedOizsfea 1021 5323 917 4639 2026(eaDe2aca) 4331 930 4213 1034 3959 970 3958 959 3919 950 3878 935 3753 2023(e3D(325xa) 3660 2025(633(325X3) 3599 1037 3516 912 3331 952 3236 2027(633(325X3) 3082 ep.eO.ep. (es®.= 0.05) 1381 SO. ao.%= 18.79 4.1.13 LVP 71 (LVP 45 eaeo LVP46/LC/1) - LVP 45 LVP 46 epzrfeoep S)jg® Q<3 Sgzsteeiza E>& csszsxDgzsf ©zaddscozj* rste&f&i e#a>3© ®25f@a% ep<>23 2 A, eaeo 5N cs3za*z» ©dscazS® Qsfta dzrfzagd -(1994) eo(3 epedOzg zsteeiza 15 23 eaeo 633623 23'®63*2ac32a'@05' eeedQzg 9 Qza SqxsOzsf c^ 2s*e)3 epjza. 70 9 OcoO: 25teei25)0<3 ^ es^zstesj ( Oes/ ©eozsV 28© @af 28.00^ ) zsteedzaos epedO^afca 3/12 6011 6/28 5619 11/46 5605 4/40 5518 15/40 5379 4/16 5270 14/52 5181 5/38 5025 1/9 4964 6/38 4962 14/51 4790 5/35 4651 6/2 4610 7/33 4593 2/13 4549 2026 (c33(32ac3) 4514 6/23 4486 13/30 4423 4/4 4371 15/51 4354 4/21 4334 6/24 4281 5/34 4270 3/3*8 4196 6/13 4092 3/11 4018 6/14 3932 3/15 3774 5/21 3731 6/36 3721 15/48 3706 6/29 3666 8/75 3567 3/2 3556 2/4 3375 6/32 3172 2027 (OO&SICS) 3053 «p.@£>.cp. (ra®.= 0.05) 2066 S O . % = 20.08 4.1.14 LVP 72 (VP 39 / L a i ) - VP 39 epsfeoep £>iQ c#coa® 25tee% cfoza 2 A, caszjte) ©eftcszS® Osfta dzsfeagd - (1994) eo<3 epedOzsj (34 zsteecte) 15 25> eseo &3Q2S> 2tf<2K3ftac325tecrt ^esOzg 10 Oza OcoeOzsf 525*03 epjK>. 71 lOOcgO: asteeizaQe qdQi&iisi (Oea/ ©eozs*/ zB© ©za* zS.cpt) 2S*©63*23G3 epea'Ojzsfca 232 3712 122 3448 34 3321 52 3285 44 3244 2023(633625X3) 3076 76 2900 20 2843 112 2814 57 2776 181 2517 2025(633(325)03) 2403 261 2387 273 2306 113 2283 188 2217 2027(633(325363) 2172 5 SS233gC0d3d3, £.©25)*. 6®. £&3@e3*23), @S3*. 623* StSgZjf^d, Ol®. dsteWCSZS, 663°. C® 3 , 6e3*.a®.CI2«5§3e3 4.1.15 LVP 73 (LVP 49 /LC/1) - C3© 25tea3*z3 ef°2a 1 83323*23 ©d3&32S® 023*23 d2S*23egd -(1996) 30 cm 40cm oSs* (geoe* 12 eseo 16) oBztf SgeOgzsf e^S eaeo essotfedzg ®3eaOe ? 0332a Oe za^ eg® @c33§23 (3?. "f^ za* 253(3 epzape Sd eaOeoza* zadza (3?. 6*. @2s*. 6®. dcaeedza ,eei. 63. 62a*. ScaegzaVjd 4.2 o£)dO 4.2.1 VP 49 (VP 37 /UC/1) 03323*23 23®H)e3* 0Z3*@23* OOZ3*03©0)23 <3c3 VP 37 epsfeotp 3fg©®23* 6«>3C0Z3* 25*363*23 ££033® 03323*23 25®S)ea* Ozsfca efSgcad ©2330ea -(1990) ©§02a epOSos caOezs* 5.1.3 Se23°2a 4.2.2 VP 55 eaeo VP 56 (VP 38 /UC/1) e£G)3© 23*©eS*23 ep«23 9, 03323*23 23®a*te3* 023*23 2ae0323i@(3*-( 1991) C3£0632a* (3253*88-®S (36323*9 ©Zsidsrajza*® 30 zSza* 6830323* dzSg ©§023 epOS©ci ef23*60§3 S?§® 63§e03 2303©23* SQ03 0)23*233 6 ? . 4.2.3 VP 60 (VP 37 /UC/1) VP 37 eRa*eO§3 Sjg©®23* 633023* 2S*©(33*23 e£0)3® 23*©S&i) e?°23 10 03323*23 23®o)e3° 02a*2a 23603Z3i©6b 1992 gOsd-eSca ea^eos esejdj 23e3*e33g zsdza e?- 72 4.2.4 VP 61 (ASM. INT 88 / UC .1/1) 1988 ^ cogjsfEto GAI ASM 13d eaots QQSI ©zaidocnzrf 25te(33°25) <^ox3@ 2sf©a% ep«2a 10 2S®«)e3* Ozste) 7S>QQOZS>I@@ - (1992) gOsd-eScs es^ eco ogd? zsdosg zadsa e^. 4.2.5 VP 63 (VP 37 / UC-.2 NOR .01) e^esy^ea) Ozjfesf § d Ogzsf e^S zadcozsJ VP 37 ^sfcoep c^g©©^ gatorassf 2ste<33°25) <^ox3© ©025*0 Ozste), @25rtdop(1992) C3gdl E3>d03g 25)d 2S)dC33g ^ ZgO© Sd QATIGXSFISN e^. &S§ ©25)' e3d©ci63-€63c325)25)©c3* 6360 ©ZSsdOp 025X325* C£(3 25)dcD25*25)3 G^- 4.2.6 VP 67 (VP 39 / UC .1 1/1) VP 39 QSKRXP Sj§©@25* eStocecs* zsfseiz?) epjeocS® 25*©d2a ep°25) 10 ©325*25) 2s>®®ed' O25*® 25)<30325)j©(3* - (1993) 03625) 25tee3°25)&3 025) TRI 2025 O 0©3 QDQLQ GobgZSf 25f®e3*25) 42 25) ^ tesOza, 1 1 025) OcDsD ^25*03 ^25). 11 0©0: 25*©eJz5)Oe epedOjzrfzs) (Oes/ ©eozsf/ zB© ©25* zS.cpi) 25f®ei25)cS gesOjzrfza 245 3892 295 3631 258 3487 265 3473 97 3456 254 3337 238 3302 332 3271 159 3192 267 3165 286 3088 322 3061 268 3045 48 2993 281 2986 273 2964 224 2950 325 2902 178 2887 252 2878 197 2857 271 2840 316 2819 128 2815 261 2809 121 2778 130 2749 292 2743 39 2716 233 2703 73 288 2679 275 2653 338 2648 278 2648 28 2634 219 2628 263 2608 20 2606 250 2601 165 2587 318 2569 277 2558 2025(03(323X3) 2528 4.2.7 VP 69 (ASM . INT .88 / UC 2/1) 1988 eo£25J03 gffif ASM § d 03323 Qgzsf QSKK&SS 2aft3(3ft3 epzeoc3® 2sfe>efe) 3023 10 caszsfcs 23®Q)ed 02sfe» 25)(30323l2sV 38© ©23' 255.q#) 25te(33'23c3 23 3111 24 3035 03(32303 1 2723 03(325X3 2 2608 5 2475 34 2449 14 2377 O3(323o3 3 2335 q.sO.g. (es® = 0.05) 1183 3 0 . 63.. %= 29.64 4.2.8VP 70 (VP 39 AJC .NOR / l ) VP 39 epsSaxp eJige©??* eSscorf 23^33*23 <#o>3® ©Ozsf© e^ ste) ©23*5gS (1993) <§>co(3 3ed©25j eSscxsf zsfeeiza 07 23 03(323 £>(3 epedDz?) 13 Qz3 QODSD 52S©3 cp?23. 74 13 0cgO: zsfee^zaOe epedQizsfza ( Oes/ ©cozs?/ zS© ©zsf zS.rat) cpedO z^ste) 299 3121 295 2805 2025(oDezao3) 2604 278 2547 46 2506 38 2492 303 2407 49 2348 N2 (e33<3zac3) 2365 4.2.9 VP 71 (VP 39 /UC .2 / l ) VP 39 cpsfCO^ Sfg@®2jf eS)3C0253b zstee^za e#ox3@ (1994) eaejdt za e?- zste^za epoza 14 caazriza zaSoJes' Ozjfta zaeODSs^ee* - 4.2.10 VP 72 (VP 43/UC / l ) VP 43 cpsfeoep S jg©®^ eSbcszsf zstegiza e^ cs>3© 25tea% ep<>z» 14 ©szsfo zs>®S)ed' Ozsfza zae©32s>J@e' - (1994) esejdj c_g ©zag©® zazrfOosO ©csza £>za 4.2.11 VP 73 (VP 44/UC A ) VP 44 cszatotp S^g©®*** eSKJjzrf 2steeiza <^e>3@ zateafo) efosa 14 ewzsfza 25>®o)ed' Ozsfca zae©325>£<3d - (1994) ©gdj $Q ©zag©® zazsfOcaO ©oza £>za e?- 4.2.12 VP 75 (VP 45 /UC A ) VP 45 cfzjSeoep Sf§©@za b eSbcozj* zsteeiza c^ cocS® zstea&a ef°za 14 ewzsfza zstSSed Ozsfza zaeOszsjse* - (1995) 35 cm (epeoe' 14) CJB&S OEFSI Oe zatcg® ©c33c.za e?- 4.2.13 VP 76 (VP 52 /UC A ) zadgseMza &>X5I®QIQSQ Ozg Ogza* 3d o^za" Oga* e«>3Cozsf VP 52 3 d ©32a Ogza* ©zaddacozsf zsteeiza epjeo3® zsf®a% epoza 14 ©szsfza 2a®«te3* Ozsfza zaeOazoiee* - (1995) ogd; ©zstezaiSd ©3e3©ci? ©DO® Z S O D oad SQOza e?. 4.2.14 VP 77 (VP 58 /UC A ) zadCj©e33za c^©^de Pz? Ogzsf 3 d C 6 3 ^ 0§za' ea*>cozsf VP 58 3 d casza Ogza* ©zsrfdsrozsf zsfeeiza efio3© zsteadza ep<>2a 14 ©3zsfza zaSSed Ozsfza z&eOozazed - (1996) esgdj; ®zsf@za3n)d ®3e3©ci? ©DO© zaos OD£> fiQOza e?- 4.2.15 VP 79 (LVP 49 /UC A ) zadgeesDza CI°X$I®QIG\3 Ozg Ogzsf 3 d Ogzsf gancnzs? 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Oocg eos ©odas-eej epocacsO oj©-@£> oes6 cases eos ©osseosd 8g£)§ 2sdi-gg £0jc;*dc3. ®ed©2s§cs 0253 es®sco©® M. epS. cf®dSoeo taeo 3 . 3. 2§ed3 cS23 ®eo2g25f ®2sf©233o)d 0 6 023 ?23 03=09 6 5 3 09323 ©odas-etj cfooscsO Oi®-66 §©63*325? ©2s?©2s? ©osseosd 8 § S § e?23sScE) cf2s?eo§s S^g® epd©eo 285® e3©3-gScD©c325Jb esszsDds 233c&@8: Hettiarachchi, L.S.K. and Gunaratne G.P. (1997). Identification of magnesium deficiency symptoms : Leaf analysis as a guid in tea crop nutrition. TRI Up-date 2(1), 4. Zoyza A.K.N., Loganathan P. and Hedley, M.J. (1997). A technnique for studying rhizosphere processes in tree crops: soil phosphorus depletion around camelia (Camellia japonica L.) roots. Plant and Soil, 190: 253-265. ©eoSSepdDS 6(3\£>es\@2s\, (1997) . ®zs? £>COD£> es^ eoo ©©3@coDd, zaaSzad® coo §>£>© cp®32a)3Do(S3©cct doSss ©ooeeood ©cfsa® zsodcsoeca ©cBzsf o e sag ©ooeeood ©QggsiO qpzjJSJjezsJ 2s»aS&3 ea^eoo. 19-30 80,. 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T3 = 900 dB (NH4)2 SO4/450 EMUaeri GS&LD LDesstemii-esi 3.6V8.&UU1I® eniuaerflev 6 eunrj (a^rfliuQ&j N erf)^3,N 3.01B T4 = 900 (NH4)2 SO4/450 «s)/_/«6ff e5<5ib roggsrggi;t_ear «6U<*«L/L/L1(51 emu oerfleb 8 eunrj (O^rflniQeunerfl^ANOOLB T5 = 125 UFFL&C/t50 emiiaeir eiloib tosmewfiueisr aeo&OAJUL-Q emuoertleo 6 eunrj (S^rfltuQeunerfl^anaaiJD. T6 = 225 d9 u&\B\L/i50 estuoxir EFI&LB i06gaf6W)/t_6gr 3>uuL-L. io«5w. 6 o//rf/ (^rfltuGleunerfl^anaau) wrr£0RJLB T8 = 6tBU«6rf)«j un0o,OUULIL_ loessr 8 a/rrrj <&rflwQ6unerf){Jr>9>NO,3IIB LDng>0nib T9 = oilQiuunQ (Q&nilL. 6ULQ6H~>LO) T10 = slunrila-sxtflastuLSL MeBr LflrjCStunaib <%LrjLbu Qeues)603.eri rsetauQuiprr)j QgnyStpunQioerr 1998 (LP&ju@0uS\eo iSlrjQiunoJa&uuQlLb. LNI - (Me Br 19) - <3<5uS)«»aj ^Q/panssor, <3(5 gourpemosiroto, ufr^aauuLL r/>nr wno,0NIB ®<$ir>3fi6\> ^i&aa ojnuee>LDuuQ0§jeu^ip^ ery80ierflo,&eri3,0ey]ib. 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QgnydpunQaen •. 3C x 4K x 3R 3C = 3 (/p60)677a76B)eu pemesaruileb WESSRESSTESIL-atgibQunQgaj ^rjibuiDnesr QanuSpurrQ sen sjessrL.rhiserr njC® 2 LDrTaj&gmulesr ®i$eunrj&0nr&sn<$essrssrrevib Q&wQt-icsurilao (LF>ISLEUES>L.i)y§j ^SQL.RRURF\ao aesrigisen Q&nCu QQ/. ®st>. 02, LEV tflrflaflev RFJI-i'.iuCi-^i. ®so)Q/ erQpwrrprrsslui S E I S L . &JULEU&06b [SL.UUCQ (6p(Q LDQJ QsiiiestsuSleo ep^ QSNL^rbunCLH.eo}6n6n 15 sesrrrysen Gefrsests f^O ung>iBu$eo) ulesremr^ib QSRRLFL purr® sen Qeuerf)ssens@6b ^ L . U U C U § J . T 1 = T 2 0 0 (253) + 0.0 43 MOP/sesrnjjprjQiurrstb T2 = T200 (2543) + 1.5 43 MOP/xmnjj/JIrjGiunsib T 3 = T 2 0 0 (25.50.) + 3.0 43 MOPjkebrQi/LflrjQurrsLb T4 = T200 (25dB) + 4.5 43 MOV/sesrrr)iju~lrjQiurrsib f^O S-rju iJIdrjuirrsiD ULQ&iburtlev QsnQissuuCusj. E290 - Qauflemev s&IPANESSR. QS^L/S ID&4)UJ iSlrjGss rBesxsvuiw, *£irB&>rres)6SR @j)GIL E289 EAR ic/ry Gisuiests SMIpeswssrssneoib (St&ibGii-iburrleo (//>L9_»/«»L/5gj seism)-, *3ir53>rres>ESR&i QgrrCL. 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Qftrj&muesr Q&rriflpurrQisen : Tl = ^iCeowuirr SL 200 500 u5taS/S)a/D T2 = ^iCeaiDUjn SL 200 750 uSeS/Sim) T3 = erssevseiv 25 EC 1000 LpleS^io/n T4 = ersseoseh 25 EC 2000 I£teS/OI6IM T5 = (g/CSenrremu rrjlQurreiv 20 EC 4,500 LdeS/ol&jD T6 = <^(3errrres>u plQurreiv 20 EC 1,500 iBeS/Sifitr) T7 = sC®uLin® ^ilL_aj6(DS5!r 1 - 30 QfftS QPFF>(B fBsDflruSlsu ffijrrffrfl g|6Umr «T«5!r65jfl«SD)ffi (CT«tw«rfl««D«/LD[n9rfl = 30) Q^IT^lrpUfr© 19 21 15 18 20 22 25 ,31*. 4 Off. g«r . «TU. Hj,«w. J5&I P2 PI Tl 3.3 3.6 2.8 3.0 3.7 4.4 4.4 T2 3.2 3.8 2.5 3.2 3.5 4.5 4.0 T3 4.3 4.4 2.2 3.5 4.1 4.8 4.3 T4 3.2 3.6 1.8 1.9 3.1 3.3 3.3 T5 3.0 4.0 1.6 2.8 3.1 3.6 3.4 T6 3.3 3.3 1.7 2.6 3.5 3.6 4.3 T7 3.5 4.0 1.8 2.4 3.0 4.1 3.6 T8 4.3 3.3 2.6 3.4 4.0 5.2 4.2 61 |^L.i_fiU6s>stir 2 - 3 0 Off if! 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QseveSI eqnin&Sl Qemeunolriis,. 0aie>jm&\£lfi{ a i iug juss r s,ss\ss)wuuins>(9)\si Qurr^Lacrft tor udPrraj,, Q - (1992) asueunSi&j QeuLiu^iuLSIesr Ga^r§ 2025, uj.0 lebt eLHjT,L-nff>&> eJlemenooev ^LLueueaem lev grjuuLLQetjetTgj. |^ili_£u«D«ssr t -oSl6iDu®ir>§iu> oSlsmsnsq (tpcnxnajflDS •SSU&JITJBgl (lp/£6U QJlUgJ <3b & 2025 if |0 1 2 2435 2488 3 2823 2367 4 2722 2175 7 542 382 LSD(P=0.05) 440 201 C V % 15 8 gjesreurfl (Lpaeb ejurjev euemrj 7euaj eu(§L. Qfleaenaaevrr^Lb. rsi—uu ^Lesmu).eb leugj a / (gi_ aesTrryaen (f/j aaeJIeveoiev. <^mene>i&no,3,erf)Gvjtb 2eugj (Lp&ev 4eu§j eu(fT,i_w euetnrj eJles>en33ev eJ}^,0uiri3LDleves>ev. scusurr/jsj Qaji_i_(y>n(i§!BQs>®s>5,nir>s$\6tsina} (Sam urruQurr^afl so «jrj)u@ib SIS1«Q>CIT«L| eilfeoienooGo ^IL.L.eues>em 2eb &nuuLLQietierr§j. |^L.L.aj«B>6xr 2 -sSl6B>cir<7<76u (•fiCcorr/Qf^D) 1. LorftfrLD Qarr(LprfjQaQo,s,n3j rfjtgigdbeb 2006 2. tx>n&Lb QanfiprsQoQaanaj 0gu^3,ev 2061 &,eueun&t&j QeutLQu'o Qurr(Lpgj Gun0uj LorruQumQeri ^(g/5£65)iou//T6ju> Csurr mrruQuiTrr^eir C<7ili_sa6a>6uc5 (3J5.TL.I_LD (,3)&j£rr«iT_ urflCffnsjsrosDT) un9,0oxif\eo ^fAGunurr 96eb Q&,n(igrf,Gi &Qi 3,3uuiluesr. (ipaeoneuaj eutfijL- edlenetiff&ev 9^/(_!.(_61/65)6537 3cu 3,nUULiQienefl 3J. 80 Gl^rr^ljburT® oSlcaxnffffcu 1. ton gib QsfT(Lpf^elejQlaarT§j r§nrng,g,6v 2364 2. LDrrgib Qan^ftZigQi&iarT&j rgtruggeb 2348 @65)L.^/flTj^cu/T6U eSlefoenff&eb sipny ^lgl&rflg,g,§j. geupsnsma semiinj&erflev san^grj^ LOilL-tb uslfraileb GJ jpuQggtb eJI&netTby (Qg^rS 2025) QamaSlrh senrjeeSleo 3 eanigrjff taCi-ihiaen (0,52.5, 105 ppm) urfl(Sfng?asuJuL-L.6sr. Pmax, 6£eiTluLin&j&n6GT glipest. gesremLDiurraaeb Qumgerr uSrreii flfrGmuSI&auULLL.6SI. ^ )es>cu &s>nyg,rjeesftebr wnrplecl 29% 2.6. 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CGUSLADO) £.I_(j)QN)RR s^ij1uf^ JU) QurrppT^LUJIJ Curr90j>«nriL|U) 3.1 AAJAJRRGGJ QQJL.19.HJIS1«DT \Esisr(j}u> gjsrflrruu^lcu QurrrbpT^luJ5<£l«T OSUDHIT&J, GL&ARRI) SN.II>«T0 C ^ m l i _ L b - ( 1 9 9 3 ) rf>L-uu 6U(njL_ ^ny&iuneia® eiilemenssA) ^/ili_aj«BS5a7 4eb gijuuCQtenen^}. |^L.i_ai«o)esnr 4 - «ula>«rrffff6b (SQmn/Q^o) iS)(T£rr«w ClgrT($(i)urr© (^t) aebearrgmjafg, (ip&si 2:1 2404 1:1 2460 1:2 2598 1:3 2911 (^0 3XU&]nS>§J3>(3)U ul&S! 2:1 2592 1: 1 2746 1:2 2461 1:3 2590 (®) 3xue>jrr&,§j&(&) (ifissr6S)nb uleaesfiw 2: 1 2448 1: 1 2472 1:2 2612 1:3 2729 LSD (P = 0.05) NS CV (%) 9 LSIcKSiurraa avnecgglp^Lb QojGeuigj N:K &J)dlgrbi a^a^LDlemuQiu eJ)&nen# &60l&j 3.2 glsrru) GjBt&GDSO e.L-jj)Qtf)tt$sS\weS\eb Quntt)n8~\uiiD Gun&msBi ( C j s ^ j f ) 2025 , (ipee>6n&ies>a;a^Lb g&i ro&sxsanu'ileb 12 ton gib L}fig,& Qftug&sr. 6peif)g,G)grT(3jLbu, euetin&el ^lenesteuoen Q&iuiuuuCL-m. (^iCt-eu&smsi 5). 81 ^|L.i_r 5 - Qimn&&\ |^srr6S)£U6B«rr @I«Devluiemeii trysnrp ^31S sic s,njs,sj. 1 2_iL»/T£6£ QunpfonSuj LOL.L.g>Seb ^mnsmsjeJlL. ULS 1 <D[5$> QiennsSemujs, snsssj. SUJOL l^ (g,6TOff)LJL| (jp<0rrj5/£ <3/£i£l6u>6uuSlsu a j rDi l^ la (g,so(rjiJL| (C$^ , j§ l 2 0 2 5 ) - Qffwrj) Sn-Lbsru CgmLi-ib - 1995 (LpSnrss Qsiuflemeuuileisr 'SrSest uSrjsSeb' ^eSliuiolhuu erSrfl ulnQiunSssuuiiQ) eJlemenssev ^IL-L-euemetssr 6eb s,nuuL.@ehensj. ^lilt-aiADSHr 6 - aSlctDcirffffsu (aKJsurr/Glsjo)) Qgir$jbuiT© oSlsiB«r[ffff6b(«l(S«orr/Q«jiD) 1. sCQuun® (?#T eJISroeb) 5417 2. /^/rJesr iSnsSesr (1%) 5555 3. SrSesr iBrjsSeei ( 1 % ) + QunrprpnSiuib ^QennemrjC® (1%) +u//fliurT (1%) 5063 4 . QuntprpnSujLb tgCBennemrjilQ (1%) + ujrfluin (H) 4988 LSD(P = 0.05) 463 CV (%) 5 ®eueu(iijL.&0ev unsjSinnesi eupilS snesm'juL.ns,evnev Srfieir tSnsSeb eJlemen&sedleb Quijjjib ejlemenemeu er puQs&jeulebemev. QuntppnStuLb ^QennemnCQ) eJlemenssemevs (gjemtp^sisj. 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Q&iucu A/CLON - (jpsDStrojsroffi |^iSlaSl(j55£9 Qaiueb 0tlL.£g6ts>Gueun efil. aesaftpann^n 2.1 Ui~(Ui6a>6ns_«o>6n>«T_i6B><5 s_lco>g . 2 .1.1 VP 52 - anrfjauQunesiem, asaniSeueo eJlemaa, (Sanil<_riiaeifleul(Q0aj QupuuCu uevtLpemen &J}es)g,aerilem LD0UIJ(£I - (1990) VP 58 - u6b(Lpet!>eTT eiflsnaaerflebr LO0utS® - QeuerilaaerrLb §}ev. 9, Qoemp &n.Loeiv (SantlL-Lb, a,eoeunaQ an&jmev - (1991) ^eug&na 1-4.2.13,4.2.14 gju unhaaeqib. 3. aiLQuurril® asuuLju _ln)UL|u urfl.&rusaDJsr 1. 1980/1981 eii aeouulanu tJlpuq (VP 39). 2. 1982/1983 em aevuiSlemu ulpuLi (VP 43). 3. 1984 em aevui3emu tSlpOq (VP 44). 4. 1985^986 6or aeoutSlmu tSlpuq (VP 45). 3 . 1 . VP39 - 1980/1981 aevuiSlemu LSIpuqaertlevl^rsaj Qupuuilu n^pQiaaeiflesi ID0UI3® VP 67,70 ,71 , LVP 66,72 (4.2.6,4.2.8,4.2.9,4.1.10,4.1.14 ^altueupevp (LpesipQiu unnaaeuib) 3 . 2 . VP43 - 1982/1983 aevuiSlemu iSlpuqaerD&SltQiiaj QupuutlL. nyrprgjaaetfleir LD0UL3® VP 72, LVP 67 (4.2.10,4.1.11 (LpempQtu zeugma I). 3 . 3 . VP44 - 1984 em aevuqu iSlpuqaerilevl{QrBaj QupuutlL. n>Tr prgjaaerflea w0ui3® VP73,LVP68(4.2.12,4.1.12(Lp6B)p(?iu ^eu0saa I). 84 3.4 . VP45 - 1985 est aeouiSlestu i^puLfaxtileSt^fBgu Qupi'iuilu PRTPRRJIAAERFLEIR LD@UL5Q VP 75, (4.2.13, ^EU&estg, I). Gurrgj anBaoliun esiaQasresta-etflesr £D(/j,//iE)L«6W, (LPLBWUL3,en QamujuuCi-esi. « . saaQesresrek) (Lpesteneuecas wan $3,3,311 usir <5. 330n(3jBLin i\3,a\BN AEVUULESNB QSUJUJUUL.L- eressesdDAeiasiUJLb e>n\u ssjeSgxipib &Gip grjuuCQtefiengj. TRI 777 48 10.3 DTI 29 7.5 HS IOA (IJFWWIIL) 160 7.5 TRI 2025 32 15.6 3N£eolujrr aaeistigfiunesmj Qurprorrrjna HS 10A (Lpetaetreueatauilm 230 yUUL.i-3ULb 0.86% 3HTUJ3,Gerr QupuuiLueer. 3>6ST iD3,NRF>3j3 Gafraesia fflev Stpfug (Lpeaeneues>3,3Xif\&SR §JTIU ^esrfsisieiaenu G)u/7Tja/£rfj@ y««6ir esiuuSeo ^ L U U L ® @ftuj!P6TS)3;ujn& 3^6M-ic3>rji\=>3>9> sevuulip^ @J1LUU($ILD &mh&6II Giurr}UUL.eJleves>EU. (tfiee>eneuee)3;3II§ITJLB y« TRI 2024 103 TRI 2025 10 TRI 777 50 TRI 2043 50 DT 95 75 TRI2142 10 ASM 4/10 20 4. (ipaDmaima ff_E/5^la«ro«niJ urfilrfl/i/ssu (,9|su/i«D<5 I) 4.1. £\pu LSlirGffjffLO 4.1.1. LVP55(VP30/LC/1)-6TuBsru6U(J), ^ujGaneixsssiil Ga,mLL.(ip&s>en6u&SI3I, iB&ud® (LVP30-QsesOtunm -1983). ajDeir GurnL GarrCt-Lb. Qaeatliunuj - (1991) ^iai£sis>3> II eb 5.2.3. 4.1.2. LVP56(LVP37/LC/1)VP37 (Glaeirrb 3Kibeh) Qftesr (ipaaarjenema LD&ULSQ, QeuetflaaetiLD £§)aj. 5. Qaesrip QggnadlLb G^TLLLO. ^rjaSeenjrfl - (1992). 4.1.3. LVP 57 (LVP 42/LC/l) urrrjibGuialeb /5/TL.L.UULIL ueb(LPE$>eti ealeis>3iu3leb)(30aj QupuuilL. (Lpessen enema logluiSQ (LVP 42) QeuerilaenLb jg)cu. 5 Qaesrip G$grrad~lLb GgmlL-LB. @t(j£3>)eitrurf) - (1992). 4.1.4. LVP58(LVP28/LC/1) - eruSlervevLSI eiilemauileolirrjriaj QupuuilL. (Lpemerreuema LD4}UL5($I (LVP28. 1983, Qaesrip Ggtrraalib G 3>ml i—th) Qeuerflaaerrtb J§)o>. 5, Qaesrip G^nadlib GanLLuLb. Qrja@ESRLIRFL - (1992) 85 4 . 1 . 5 . LVP59- QupvfiiiunasGGo @ itT,qfi&s>enajes>a; oi)anesr 0n}jeuuuL.L.aj. (^eij&jsmy) 11,5.2.4). 4 . 1 . 6 . LVP61 (LVP42/LC/1) - urrijinduuSleisr (LVP42) ueb(ipesien aflao^uflesr (Lp&steneueetsi ID&ULS®, Qav. @ 6 u . 2 A, 5 N, Qseirip Ggrr&ifitb G&niLL-Lb. Qjrjaig&sTurfl - (1993) anLQiuunQt TRI 2023 £_(_6W uS ^©LO 20 (LfsesieneuesiaiaxifleSsr eifl&aensffeb ^Itlueusmsm 2«J grruuLiQienengu. |^tli_ai6B>mffffw (sICwrr/Qam/su^) (lp6S)6nS116B)a 186 7514 160 6624 243 5951 253 5883 281 5864 285 5585 375 5464 342 5320 171 5226 168 5213 289 5080 298 5003 109 4996 119 4858 322 4786 10 4752 173 4614 169 4556 107 4481 66 4446 2023 (xCQuun®) 4437 LSD (P = 0.05) 3330 CV % = 29.75 4 . 1 . 7 . LVP62(LVP45,LVP46/LC/l)Q*6*nb Ggn&Sw GgrrCL. (LVP45, 1989,LVP46, 1990)Qeu. g)eu. 2 A, 5 N , Qfesrip (SgnaallLb G$/T(i(_(b. Qjrjgglesruril - (1993) aCQiuunCQi (ipemeneiima TRI 2023 gjafl/_ 17 (ipesien&j&si&aen <3\4}a\ aJ)«s>a7#^s»6i><5 &,rf>&,Gm (^iCt-eu&Bem 3). <3)L-i_&]«D«Hsr 3 -(jfor6U6S)s aSl6axrr&&6u (aKS«orr/GlsjiD/aJ(irj) (ip«0)«rrai6g>£s aSWrneFffsu 63 5516 11 4426 59 4349 47 4090 12 3855 2025 (aCQiuurr®) 3715 13 3692 86 (jp6iraj& ~ afflsRxnff «F«U 64 3629 23 3624 49 3607 52 3565 54 3416 51 3294 55 3153 72 3037 36 2945 2027 (aCQiuurrQi) 2715 58 2703 10 2668 2023 (atlQuunQ) 2330. 67 2169 70 2147 53 1934 30 1386 LSD (p = 0.05) 3053 CV % = 40.37 4.1.8. LVP 63 - QuppltiirTaemev ®(g(i/)S5)677Qjss)« ai)«»s>(Qf)LD aCQiuunQien^Lb grjuuCQienenew. ^|ili_ajaDswr 4 -(ip6naico>s oSloDcn&CFeu (dPCsu[T/G$fD/«u(!5) (ipoDcrr6U6D>a 19/2 10397 4/2 9106 21/23 8744 2023 (mCQuunQi) 6836 8/9 6293 30/7 6012 19/1 5926 1/2 5756 8/8 5654 2025 (xLLQuunQ) 5545 14/17 5477 29/22 5409 31/1 5409 11/11 5387 25/36 5193 23/16 5174 23/22 5173 15/18 5131 14/19 5097 2027 (aCQuun®) 4883 LSD (P = 0.05) 3556 CV % = 32.45 4.1.9. LVP 64 (ASM. INT. 88/LC/l) ASM 1988 ^pl(ipaiiDnesi eJlemguSlevl^r^aj QupuutlL. (Lpesmieu&na, LD^ULSQ. Qeu. ® a j . 2A Qeeirp GgrT&JHiJD QaimluLb. Qjrj&j&esrurtl - (1993) ^liLL-Gumem 5eo 33 (Lp&np Q&fT(opffig@g,&,L3ebr 8 (jjiemeneuemaavenfLb. s,CQiuunQ\w grruutlQtenenesi. 87 |^L-L.«ucB>6nr 5 - (ipctDcnsuctDaacrr1«Br sS[sB>sn&&so (dOCwrr/Qpaj/aj^) (ip«o«n£ff/ai(irj) SlSlsBSITffffSU 2026 (aCQuunQt) 2023 (aCQuurrQi) 113 267 95 129 33 38 222 2027 (anLQuun®) LSD (P = 0.05) C V % = 33.00 6478 6016 5665 5115 4847 4665 4431 4292 4123 4100 2786 4.1.11. LVP67(VP43/LC/1) VP43 (LpemneuesHU w&uu'® Qeu. g)cu. 2A. Q&eirp QgnaSib (S$!TILL.W. @jrjg,0esrqrf) - (1994) <3|iii_aJ6a>ctir 7 - ttpeoeitaiaaa eReaeneew (dPG«urr/Q«MD/aj(|5) (ipcaxrraiaDS sS\sis>ai&esi} 814 7301 2023 (sulQuurrQ) 6003 710 5032 571 4539 759 4480 655 4440 708 4399 2025 (aCQuun®) 4367 679 4363 88 (yXB)Cff61J6B)& 626 4254 757 4095 709 3996 554 3959 628 3943 840 3935 769 3932 685 3896 627 3828 621 3787 661 3749 612 3728 657 3706 624 3683 588 3624 770 3588 768 3520 504 3463 763 3434 557 3368 745 3348 702 3339 735 3262 744 3255 2027 (ailQuurrQ) 3239 LSD (P = 0.05) 4009 C V % = 46.31 4 . 1 . 1 2 . LVP 68 (LVP 44/LC/l) VP 44 (rpesterreuesta wgudQt. Qeu. @su. 2A, Qaesrp G&n&&u> (1994) ,=9/LLSJ 65)6937 860 11 2-iufr 6i!)60)677#^ 6u (jjietnetreuemaateiTjib aCQuunQaeiflesrajLh oilmen ease gnuuLQenenaj. (y)6roerr6ijsroa5 1021 5323 917 4639 2026 (ailQuurrfJi) 4331 930 4213 1034 3959 970 3958 959 3919 950 3878 935 3753 2023 (aCQuurr®) 3660 2025 (aCQuurr®) 3599 1037 3516 912 3331 952 3236 2027 (aCQuurr®) 3082 (LSD (P=0.05) 1381 C V % = 18.79 4.1.13. LVP71 (LVP45,LVP46A-C/I)u6U(i/365)6ffaj«i»« eulesis, (tpesieneueaaaeruesr LDSULSQI. LVP45.LUP46. Qeu. £§)6u. 2A, 5 N , Qaesrip GgrraSib GarrL.L.Lb, {£jrjag6sruiil - (1994) 89 /^L/_QJ60)SSOT 9ev 15 P uifr eiUmeniffeb (ip&s>en&i&aesfTQJ6DS aSloDcrrfffftu (s1(eC0ir/Q$jg)/aj(J3) Qpsomaima eSlas>m&&sb 3/12 6011 6/28 5619 11/46 5605 4/40 5518 15/40 5379 4/16 5270 14/52 5181 5/38 5025 1/9 4964 6/38 4962 14/51 4790 5/35 4651 6/2 4610 7/33 4549 2/13 4549 2026 (aCQuuti®) 4514 6/23 4486 13/30 4423 4/4 4371 15/51 4354 4/21 4334 6/24 4281 5/34 4196 3/38 4196 6/13 4092 3/11 4018 6/14 3932 3/15 3774 5/21 3731 6/36 3721 15/48 3706 6/29 3666 8/75 3567 3/2 3556 2/4 3375 6/32 3172 2027 (aCQuun®) 3053 LSD (P = 0.05) 2066 CV % = 20.08 4 .1 .14 . LVP72 (VP39/LC/l)- VP39 (£/j«B677a/6S)« Logud® Qeu. g)su. 2A, Qsebrp (SgjnadlLb <3$nCL.Lb, Qjrjg^eisrurfl - (1994) ^/Lli_eues>es97 lOeb 14 &.iun aSleiaenooeb (Lpes>eneuG®&&erfleisrffliLb aCQuunCiSLesigjiD etSesienooeb grjuuilQienengj. 90 ripemsneuem& 232 3712 122 3448 34 3321 52 3285 44 3244 2023 (aC®uun®) 3076 76 2900 20 2843 112 2814 57 2776 181 2517 2025 (aC®uun®) 2403 261 2387 273 2306 113 2283 188 2217 2027 (aC®uun®) 2172 LVP 73 (LVP49/LC/T) - sen.fr uHleuev. anisauQunemesr (Lpemeneuema Qa,freu LDSULS®, Qeu. @J6v.l. Qaesrip GfgnaSib (SamluLb. QftrjaSGBrLjii) - (1996) aesrrQiaen 30. 40 QaiS (12. 16 ^//5/@cutb) P-iun^Seb u^esfleojLD ^aehjLiuLeojiJD QeuilL-UuC® Semen aerf)esr rrrj rgemp u0tuuuCL-Oj. Cuxu SlrjGg&lD VP 49 (VP 37/UC/I) - VP 37 (Lpemeneuema LDSULS® (Qaesrip a^Lbeiv)^J60LtSeulujfr iJlifleuleb. Qaesrip OKLberv - (1990) «3l6U&/sm&> II 5.1.3. VP 55. VP 56 (VP 38/UC/1) eru'iehjeviJI. ^jaiQanevemL. ^injanemesr eulemavileolirrjrsaj QurouuCL. (ipemeneuema (3a Qajreu ajessrL-iiiaen QjesreulirjjgS Qad]uuu®SesT fpesr. VP 60 (VP 37/UC/l) VP 37 (Lpemeneuema loSutf®. Qeu. g)cu. 10. Qaesrip a^ibeiv - (1992). QauLaen aeiieuna>as QeuC.L-uuC®enenesr. VP61 (ASM.INT.88/UC.1/I)ASM 1 9 9 8 , 5 / $ ( i p a edema (Lpemeneuema LDSUIS®. Qeu. g)«su. 10. Qaesrip ah.ibehj (oajrCL.iD a,eveunaQanebemeo - (1992) QauLaen aeueungaj QeuL.L.uuL®enen6sr. VP 63 (VP 37/UC - 2, NOR/I) VP 37, eruietveoiSI eSlema. Qeuesrah. Qnjnfreu,L. (Lpemeneuema LDSUIS® - (1992). QaisLaeh aeueunaaj QeuiluuuC®. QeuilULUj Semen aen {SipiaauuC® TRI uiley/LD Qnyrheuji. Ga,mlL-g><§eojih ajessTL-rsiaerr ^esrQu{njaauu®Sesrpesr. VP 67 (VP 39/UC 1/1) VP 39 (Lpemeneuema LD^UJ®. Qeu. ggjcu. 10. Qaesrip a^inerv - (1993). aL.®uunL® (ipemeneuema TRI 2025 «y/Lb ^Saiona eulemenaaemeo^, 8>ft>a> 42 (Lpemeneueaaaerrlesr eulemenaaeb <3tCL.euemessi Uev a,nuuL.®6nenaj. 91 <3|ili_aj«D6ioT 11 - (ipeo>ciTSU6Q)a fiS)6B>err&&essr 126b (tpss)6r7a/«o)swr 12 - (y6»OTaj6B>««eifl«8T sS\sa>mir-9w (ffiKSwrr/QsjD/ajfjrj) (Lp60)«naJ6ro« eS\as>atir-&ai 23 3111 24 3035 aCQuun® 1 2723 onLQuurr® 2 2608 5 2475 34 2449 14 2377 aL-Quun® 3 2335 LSD (P = 0.05) 1183 CV% = 29.64 4.2.8. VP 70 (VP 39/UC.NOR/T) VP 39 Qpes>ea&ies>a ID0UI5Q), Qeuesrefr GgrrtLLLb. Gnyrfreujl - (1993) /^L.L.6UCB)SBo7 136V 7 £_i/J/T 6li)«S)6T7#ff6U (ip&S)6n&J6S)S.ff,6lfl6STgJLD &C®UUnL-lSL.6Sr§JLO af)60>OT jdFCU grjuuCQ&nengt]. ,3|ilL_aj60>swT 13 - (U3«D«naj«D««6ifl«0T «S1«D«naFffffb (a!G«urr/Qfj/D/sij(i5) (ip68)6rrSll60)fi& 299 3121 295 2805 2025 (aCQuun®) 2604 278 2547 46 2506 38 2492 303 2407 49 2348 N2 (aCQuun®) 2365 4.2.9. VP71 (VP39/UC.2/I) - VP 39 ID6v - (1994) QarrcLprsQg®&aMuG)d)rp§j. 4 . 2 . 1 1 . VP73 (VP44/UC/I) - VP44 JO^UL?® Qeu. Qev. 14, Q&6erip ^ibeiv GgniLi-W, &,6V6urr&Q&.rrev6tn6v - (1994) 4 . 2 . 1 2 . VP 75 (VP45/UC/I) - VP 45 - inglCuS® Qeu. gfteo. 14; Q/resrrb ohLbeiv - (1995). QsuLosn 35 QSLE (14 <3iriigg Ggfreii. Qeu. Qjev. 12. Q&ebrii) sh.ibeiv - (1996) *3l&,GLrrutfl6v geisr® QeiiLLLUUiLQl saLQiaeirmjaerr r$LUUL.L6sr. 93 4.2.15. VP79(LVP49/UC/I)- aajpajuQurresr. sennibleueo (LVP49)afls»££ Q&nC.L.rhi&6RF)&SR ueb^p&AEN avsroa ato<5 QgrrCL-io - (1996) ^MSunurfleb 8>6SNQ QeuCuuuCQl ssaQsiimiQiaen fBL.iniLL.6sr. 5.3. ssi6urr 4.3.1. ssaeurr 8 - Q96srp &H-LBEK> QarrCL. ASM 1998 ^plfipa, &1les>9, (ipemerreuema, LO&UISCJJI. TRI ssaeurr &_U (§68)6011] LD. U 90S) IP - (1993) 5. Qpss>maise>& ufi\G&ng,85>sBi (,g|6u$so>£ III ) 5.1. CLMUlSlrjC^ fflJD 5.1.1. VP41, VP42 - Qaerrp ohLbeivfflev 60 (IP6S)erreiJ6S>9.9ieiilesr urW$9rr9/ss>esr - (1984), eurr&j&err '4000' Q8,RRL.fr ung,4)8M>en Quq^9<9jeu9,P9IRR9I QeyrtlL.maen. 9)rry&rressfl8jetij&<9j euLorii9.uuCL.9J. 5.1.2. V P 6 2 - Q&6BTP shiosrveb U£AJ 4000 Q&NL-fr (LPES>6N6U65>&&6FF)6sr urKSan&mesr. Q9,rr(LP^Q9/Ql^9,eb rBn^igsnlruilQi Q&uj^aetj &6II6>irr$,§j Q6uCL.uuilL.6SR. 5.1.3. VP80(VP37/UC/II)-VP37(y36»erTQ/s8)« ID&ijtf® (Q&6&rp ah-ibeiv) QJEOIFILEVLUJFR Lflrfta/. Q&6brp SKLDOV - (1996) ^•kQi^RRURFLEB 9,6stsiQ) Q6UCL.UUC(SI smQ&esrtr)} r£L.uuCu§j. 5.2. aSyjuiSlirC f^fLO 5.2.1. LVP43 - 0iu9,rreu9, Q&nCL-LB. Qaesfluirmieitieb 42 (ifiesieneues)9i9xrf\6sr iD&inS® - (1990) 5 .2 -2 . LVP60 - Q9,neSlfr>9,n (B9,rrCL.IB, Qaarretneoufleb 9 QFIGNENEUGNXAERN urflQ9rrayas>6SR. *3lCL.6U6G>6sst 146b (j/)«o)6T7a/65)6sir 14 - (ipcrrsu«g>s oJI 65) stiffs1 «u 2023 (aCQuurr®) 9433 4042 9074 4083 8096 4014 7756 2025 (9,C@iuurr($) 7088 4075 7056 4080 6862 4046 6834 4076 6669 4015 5703 LSD (P = 0.05) 3235 C V % = 21.96 5 . 2 . 3 . LVP 74 (LVP 30/LC/II) LVP 55 (L/)s»er7aj68)« LOOUISQI. ajoeirGurrC GarrCt-ib, Q&esfliurriu. TRI Q&,&sf)unui r§6S>6VLUIB - (1997). 94 h\P 55 unXSorrgeaieerufleir #)prr,&, (ip6s>erieii&»&,<9,6ri ^aotuiLiiLeb r3rrL.uuuCL.eaT. (Lpes)6rr eues>&, eiwusrSlaeas, - 35 grreurj eTeisreinfl&'Ma - 24 Lorgi Q&tiian&atg,) top Q&ui&na. ereiai&aSlkesis, - 02 aCQuurrC® (rpemetisii&s>&, - TRI 2026, 5 . 2 . 4 . LVP75(LVP56,57,58,59/LC/II)-LVP56,57,58,59cipa»6way60)« i D g u d ® , Qeu. Qeu. 1. Qeesrp Q^nadlLb, Qrj£gletsrLirfl - (1997) LVP 56, 57, 58, 59 Q6br flprsg (ipemerrarestoiaeir ujanevafleb nyrCuuuCuesr. (ipesmt euesis, eresaesefldeiao - 40 s,neun ereisremlaeina - 25 («£(5 top Q#«@) top Qfiuests. eressressflaema, - 02 aCQiuurrC® rrpeiaetreueaa - TRI 2023,2026,2027 5.3. ssisurr 5 . 3 . 1 . UVP 7 - TRI smeurr rfjemeouj&ifieo 38 ene>i&s)&,&eri urfKSorrgmear. eungjasn '4000' Qgrrufr (i_p&S)6ti&i&s)&,&>eiS)6n r§mje>ieiig,p(g) GajrrCuriiaLb ^p<56/T«^«(6Tjj6neues>Lbeiv GgnCub. QevuSeoluifr-tJIrfleii - (1990) eurrgjaxrr (S&inCurii&err, &lrnjarrew5na.(6tjj&(g) etJipmi&uuCueor. 6.1.2. 6fuQi_rrffiffiib C ^ m l L L b , /Jtar&js a .uj«r - (1991) (Lpesierreueaia&erileAr eulesien^aeh ^Cueumesa 15eb grjuuCQienerrgj. ^|tli_«9T 15 - (ip$cosnajsm<9B6Bsrf)m s£\6B>6n&&eti («1G«jrr/Qfj©/aj(i]j) 3020 5711 3048 5360 3016 5184 3069 4607 2025 (aCQiuurrQ) 4414 DN (aCQuurr®) 3980 3019 3826 3017 3211 3015 3098 3013 3079 3018 3024 K 145 (aCQuurr®) 3010 3014 2907 LSD (P = 0.05) 3683 CV% = 32.52 6. 6.1. 6 . 1 . 1 . 95 6.1.3. -SlTLSOT .£ITLL_Lb, _.L_LJL|<9:6USUIT6D)aj - ( l 9 9 l ) (jp&NENEUSB)&a6tfl6ir afl«o)67T<#6iT-i6roas 6_lso>«n<7<76u (SGson/Qe>JS)/eu(J^) (tp8S>fttQieta& s_l60>6rr<7ffsu 3015 4061 2025 (atLQuun®) 3760 PK2 (auLQuun®) 3628 3017 2865 DTI (ailQuurr®) 2694 3018 2613 3016 2448 6.2. __s_rr 6.2.1. Q6_(J>6ULIU£6S5«OI -grri_i__> -(jjCiorr iSlifl«_|— 1988 Q&,mLL-Lb Q&rrqpriQaQa<paj. 6.2.2. U8OTLfTrrQaji5u, fijrrjrrsolfiTsu, Qurrpsurjsag, <#lpaR<59if1acrr- ( 1 9 9 2 ) Gian(iDrsQ0®^,s,eb rgrburtili-UULL®. Q&LS^AERR aeueun^GJ QeuiLL-uuC®, rSemro uguiuuLQerreirgj. 6.2.3. T S H D A - of/jrrsolnsu - ( 1 9 9 2 ) QarrqpniQ0®§,&,eb TSHDA UJLEOTTIEB Q&,RTL.(Q<&rD§J. 6.3. LDg<§l_ \S\irGg>& ITrr«_6tD<5UUJLb 6.3.1. s\jbg>na>&( C«5fTili_ib - ( 1 9 9 1 ) 6.3.2. 2_«g,a/6u, L.60TGtjrr. QgevQgesflui. (3)(_/7(_6ara/6u, TSHDA g,&ipes>em (LPIQJ^geoneu 41rQjsrtemlaen. TSHDA (LPFQIGATGCNEIJ (tpeaien euesta ®5les>GN&&6B *9JLLL-&J6O>65SI Web ff,rjuuC®errengj. •3>t5tD6n6_60>S «Sl«D6iTnm top part*, QFUL&err aeueurr^g, O a / L L U U L L O T . 96 6.J.5. Qeu&si&fr Q&mlL-ib. Gij>itI'IB^C. Qeu. ®su. 6A-(1993) (U)«Dsrfaja fiSI«0>6IT#«F6U 4071 3662 2025 (aiLQiuun®) 3285 3069 3197 3016 3139 3073 2981 4052 2551 3019 2509 4063 2459 3072 2266 4053 2032 3020 1775 3015 1753 3018 1312 3017 719 6.1.7. &°6BT @C5'J. ijeisi®eiieties>a> eiTleineriffffeu grjuuLlQenengj. |^L.i_Qj6D>«ir is - (xpeamaistoa eS\ss)snsB6) (c^KSturr/QarD/cu^) (u>«s>6ncus s&iaxrrffeco 4078 4053 4083 4075 2025 (xLlQuurrQ) 3069 4079 4076 4071 4053 6223 5676 5538 4940 4730 4606 4016 3477 3247 2682 6.1. 6.2.1. Qeunyeouu&esr QeyrC-i-ib. (§IDTI ulrfl&i - 1988 Q&ISLAGTFLGB (2&,mLuLb Qan(Lnr^Si0®9,41ip§j. 6.2.2. UEIAL-NRJ&JGO, eunneolerev. Quntpevf&saai Snji&nes&ASN - (1992) Q«a(Lp/7jG)^(5'^£6o rSpunL-L-LiuLLL. Q&isj-AEN ae\i&in&,§j Q6U£L_UULLQ R§es>p LiSiuuuL.L.gt]. 6.2.3. TSHDA - ajDrreclsrev - (1992) Qsiq.axifleb TSHDA Qan(iprbQ&®ik&)pg,j. 6.3. IDA^T&IU ulijG&JFFID FFRJGUEVUJIB 6.3.1. ^i%aysstew (S^/tll/jd - (1991) 97 6.3.2. e_«@6U6o. JS^/JD. QgevQsiesnuj, G)/_/7L6ara/6u ^Ldlui&i . ^/LL_oyss)S5S7 29«u TSHDA (rpirji^sievrieiaeu Qfimefieuesia eSletSGfi&ffeb anjuuLQenenaj- ^|L.i_«u6n>snr t9 - (ip«a)«naj«D)ffi«srflor oSl60>ar#?rr/$a)iD/aJ(J5) (jfCBxircuaDa 4046 7366 3018 6343 4006 6468 2025 5731 3015 5615 4042 5276 3013 5147 3020 4952 3014 4742 4053 4404 3019 4159 4047 3028 LSD (P = 0.05) 3028 CV % = 25.48 433 6.4. £[p_u iSlrrCsjffii) 6.4.1. LVP 38 - euL.L.uQun&& QanLiuib - (1988) *3|L.L_&JKD6wr 20 - (jp«D«rr6iJ6ro« oS)oDsir#9su (SGoyn/Qs^tj/euijr,) 4056 5315 3020 4425 4059 5278 4054 5974 DG 39 (atLQuunQ)) 6602 4055 6958 4047 6453 KEN 16/3 5935 LSD (P=0.05) 1338 CV% = 18.48 6.4.2. 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N/K 6rglniliLi&, Gi3>m nuns. &arsgrj96ir, QunppnflujLb ajenionsdlsxisien (Lp&)nr$&, G£u5leB>eo&@ ulrflgigfju i3rjGtiin6)3>a,e\.:, Qsesip ohibeiv GSJIL-L-LO. $>6V6iinaQ&n6bes>GU (Lpesierreums Qg><5tr 8 - £lurrrJ)rrjrr^ 1iu/2«D)$ iS)itlgg$iJugrrsu G i^filsoeu aS1«D«frff?6o1(i) s;rj)u©u> aSlaowraj, (CRBUG^G, Cgiflanw/Qpo/surrj)) K fi-ITLI iS1r/<3mrrau> «r99«u i^ru>ULD 6«ugj AIFRRRG$«OT iSlsor K tiTii (ilCsufr K2 0/61fj/j)/ curjij) 120 240 100 0 864 839 80 20 905 913 60 40 849 925 40 60 875 840 20 80 936 824 0 100 874 838 LSD (P= 0.05) 78 CV% 6 uln~l&,&, ulrj(Siunsrbiaeifl6srnGevn ^evevaj QeiieiiQetirnj *2f6ne>i&6rfl6Brn(Sevn eiSles>erj&&Gul6b ^pluulilL. &Slg£uirT&L6l(rrl&,&,&flev65)ev; &lev enn&rflu Qu pjiDnmiiiseri LSD Qup/j)/765r$dj/6uyib nL.(nj&tp§j. 2.2.2. N®6oT 60 <3l4}&rf)ut.i a?'g,thi#(6757-687 (240 . 360 &Q eon/Slap fang)) S\{£i3,nl&9> Qunppnffliugesig (60 - 360 eneti. a/n 6i)Qg/76U65)6u<9i <3.?3777l/_7b, tuiiULLunrySls^iCemL. - (Lpemeneueias G$<$Lrg 2025 (1984). 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Qunppn&liu&sstgi (48 Q<9j/7i_<35#j;b 480 dKBeon/Slem/eutQ K2O Mg £_(_65);ib £§)6L>6U7TLOFVY(i) (0. 60 dUGevn/ QennjkmjT, MgO) £_7J^ 5«j/_6sr ulrjQuindluugneb inem^neun K/Mg &>e&r&s)iBLrj)eyiLD Qgufl&neVUflSST Q/)60)677# (S^ uSlsmsu eSlstDSfrffffofltu 6jii)u@ggiu> aSlctDtnaj UULL_U> (ff3C«urr/Glfja)/oj(j5) oSl«B>&> Qunprpnsluj ^lenei^s/Senrr ^ebeogj 60 dH(BevrTjQeirn/ a » © MgO ulrjQuirra^SQeoij eS&smis&eoleb @/rJuLfli l(_ efil$&um&LbleosG> uxnr K fj6ffr«a>u>i£)a) jgl^ icsssr (^preiacrflsu ldCl-lo (SQeorr/ Q&jd/ a / © ) Loem K (uSlS/SQevn) N K 2 0 MgO N : K 2 0 0-15 Qs.lS 15-30 Guf.lS 240 48 0 5:1 130 122 240 48 60 5:1 120 110 240 60 0 4:1 150 154 240 60 60 4:1 125 117 240 80 0 3:1 157 142 240 80 60 3:1 222 162 240 120 0 2:1 207 175 240 120 60 2:1 140 132 240 240 0 1:1 212 195 240 240 60 1:1 162 150 240 360 0 2:3 220 230 240 360 60 2:3 207 190 240 480 0 1:2 232 207 240 480 60 1:2 240 212 LSD (P= 0.05) 84 70 cv% 37 34 £ _ u QayrySpunQ MgO (dKSevrr/ Qew)/ a / © ) 0 187 175 60 174 153 LSD (P= 0.05) NS NS Qungueuna, £g)© ^LpmiaerfleyiLb losssi K LOL<_/57«677 r§ev K iS\o8iunff,s,0esr ^3\4}s\if\UL\L.6W *3i08.if\8,9,a]\. £ j j ) a / a / © L £ < # 6 u £jj)© ^iprsi&erlleyjiJD Mg &_rj60 M g O / o l a / r j ^ © <^<9S Mg SLCTUJ LSIrjQiurraUgs, Qi inqgg,] tosaw K LOLL^^SU (g,n$uu~l£L. eiSlesteneii sjtpu(_aflcuansu. 110 ,9|il.i_aicn>«Hr tl- GlaidiC&jp N : K 2 0 aJIdP r^aiffifjii MgO &|ii> uwwr Mg/£«or«B>LDi£l«i) icsisr sSltDdiaj LOLLLO (dlGeou/ QfUT)/ 611(1])) N K 2 0 240 48 240 48 240 60 240 60 240 80 240 80 240 120 240 120 240 240 240 240 240 360 240 360 240 480 240 480 LSD (P= 0.05) CV% e_u QanLplipun® MgO (&Geon/ Q&JD/ 6x1(3) 0 60 LSD (P= 0.05) MgO N : K 2 0 K Mg 0 5:1 165 120 60 5:1 210 160 0 4:1 182 187 60 4:1 202 183 0 3:1 254 172 60 3:1 220 214 0 2:1 185 171 60 2:1 217 171 0 1:1 213 160 60 1:1 193 199 0 2:3 198 141 60 2:3 218 213 0 1:2 196 199 60 1:2 190 213 NS NS 39 40 199 164 207 193 NS 0.026 0600060 6041G6vn/ol6>if)Jhj(§ Mg P-pu i3nGuinaaS6srnGeon *£ig)aiflfy£ QuniprryT&lui ^ten&jaeiflesrnCSeon ®(5 ^ipiiiaeiveojiD LOOTST Mg LDL.L.riiaeifl6b @nj7uLJ)tl(_ eJlaSumaLS^aaetilebestev; Mg 2_i_«p_/ib ®6V6omD60jiD LflnGuinS^a,Qun(ip§j &51£0uinffr&iaefi anessiuuiLL.6sr. N:K 2 0 eiilSa/biaiei^ib MgO a//b Qlemev Mg, K ^Suieu/prrSev ejpuQaajib aJ7«»6wa/ L^LQJSOJOTOT 126b &,rjuuilQienenaj- ,3|L-i_aj«o>6ssr 12 - QajsuCajp N:K 2 0 oSI^Jgnis^ib MgOajib @«D«U Mg g^ jib K gjjii 6j(T)u@/igiiJD LOLLLO (eUGeon/ Q&JD/ a i © ) HjemeouGunaemessr (%) N K 2 0 MgO N : K 2 0 K Mg 240 48 0 5:1 1.39 0.28 240 48 60 5:1 1.43 0.28 240 60 0 4:1 1.23 0.30 240 60 60 4:1 1.28 0.31 240 80 0 3:1 1.24 0.32 240 80 60 3:1 1:20 0.30 240 120 0 2:1 1.21 0.30 240 120 60 2:1 1.14 0.34 240 240 0 1:1 1.28 0.28 240 240 60 1:1 1.33 0.30 240 360 0 2:3 1.42 0.27 240 360 60 2:3 1.47 0.31 240 480 0 1:2 1.44 0.27 240 480 60 1:2 1.28 0.32 LSD (P= 0.05) 0.202 0.036 CV% 12 10 2-U QasniplpunQ MgO (4X2eon/ Q&JD/ eutg) 0 1.32 0.29 60 1.31 0.31 LSD (P= 0.05) NS 0.012 111 R§ev^Seb M g g 6 0 SQeon/Slevnjeinrh < ,^s,9i\. cfy/flgpyib QURJIPIPNALUJ&saa, ^GARFLGAIENEIFLGV iJ)RJ8MRT&£3IQURR(LPAIJ J^sroeu K @frjL/L/?L.^ «^«6T7a/ ^ SarflsaeiflebeGiev. ^juurfKSsrTgmeisr Q ^ L _ ( g < $ / D g j . 2.2.5.N 6U6«/D/7&, (100 ^^orfluusien) LJIRJQLURT&UUSJRT&J LOEM/ajneurj N/K getimLOuflevjLb (Sgjutosu etfl&NEN&&EOLE$jLB sjpuQiLb eiilGmen&i, Q.&6srip Gg>ibek> (B&MIL-LB, ennneol &rev-(LPES>6neues>s, Q§^j§ 2025 (1990). 26UGJ EUL-L.gj06br 2eugn 61/(31 L eifleoienffseb L-L-eu&SXSM 13eb G,rjuuL\Qehengij. ,9|ili_aj«D«iBsr 13- N £_I_«BT ^i^larfl^fj QurrpflQrreluj uulL.r&iacrr Cgi£l«n> oSlaDnraj UH1I_U) (sKSconv Osjffl/ 01(5) aSlaDaiff&cu ESLEE>EN88AI S£\SFF,W N K 2 0 N:K 2 0 (P-pu&,4) Qguflesiev 4¥Seon/ Qfun/ 6U©) 100 100 1:1 2178 200 100 2:1 2165 300 100 3:1 2289 400 100 4:1 2299 500 100 5:1 2363 100 300 1:3 2319 200 300 2:3 2265 300 300 3:3 2363 400 300 4:3 2347 500 300 5:3 2344 100 500 1:5 2284 200 500 2:5 2363 300 500 3:5 2220 400 500 4:5 2385 500 500 5:5 2216 LSD (P= 0.05) NS cv% 11 N 1 DILI—ID (SGevrr/OLFTIDFIJINJ) 100 2261 200 2264 300 2291 400 2344 500 2308 LSD (P=0,05) NS K2O LDL.T—W (&G5ldl0r5i&, ^9] Sarfl&i&GLILEBESIEU. r§sv N, GiurrpprrffluiLb toeisr K aieirsninuflevjib ^ smev N, K ufleytb ejipuQl^AJLB eiflemeneu (tpesirpQuj .=9/L./_6ij65)6587#6)7 14. 15eb s,rjLiuCQ\enen gj. 112 ,^ilL_aj«DOTsr 1 4 - N a.i_«w <3|/£lsifl$£ Qurrppir^lujib 6wr 1 5 - N a.i_«or £\0atf\8>8> QurrjijprTrflujiJB glsssu, N, K 5«OT«O>UH£1«U «rjbu@^g)ii) aSlsrowraj. N LOLLLO (&Q LOfrpn3Lo?(5<9!<95«ifl«u6tBco, (gjemev K Qanfleu ^Sani)^3i§j. ^uurfKSanoemesf QortL.fjjjSip§j. 2.3 LD655T, QuiTAUITSr ajetTLDrra&dQ ^ITfTlLlff^l 2.3.2. <3ld2arfl&&> Quno-Quiprpj LDiLL-ihiaemena, (20 <3j0&rfluu&erf)ei) 0 - 120 AXSevn P^d^oienojeu^) LSInQiunSuusjneb wes® /a,neutj P aesremtnuSleojib etSiemenaaeolevjio ejipuQiLb eSlemeneu. Glaesrrb o^Lberu G /^TLLLO. oweunaQ an ebemev - (Lpemeneuema; (SO^JS 2025 (1989) 2euoj euL-L. 3euoj eutnjL. eiSlemenaaevjLb LOOTS P LDL L /5/atenjLh (LpemrpQuj ^ilueuememoeri 16. 17ev aHjuuCQenenesr. 113 |^Li_6u«a>en99eb (dKSeorr / Own/ Q J © P 2 O S ) (a . .O^j. dUGeun/ Q&m/ a j © ) 0 2 3 8 8 2 0 2 3 8 4 4 0 2 4 4 8 6 0 2 4 3 2 8 0 2 4 3 0 1 0 0 2 3 8 7 1 2 0 2 4 3 1 L S D ( P = 0 . 0 5 ) N S C V % 7 ^9\&)9,tf\a,&, siuuneiieo unesttpu QunaGu PQILSSI euletaenssev go. ^ | L . I _ « U « D « S Y R 1 7 - , 9 | { £ L & R F L G G Q U I R S R C U J B J J J J U U L I _ R & I A « i T I D S S V P U S I S U < R R I ) L i @ £ § J I J > A S L S O S T R A I P P-FJ LDLLLD WEI® (AGeon / Qa/r j / a j © P 2 0 5 . ) 0-15 Q9.i£ 15-30 Qe.iE 0 2 3 . 3 2 4 . 7 2 0 1 5 . 2 9 . 2 4 0 2 6 . 7 3 3 . 4 6 0 3 4 . 3 2 0 . 8 8 0 5 5 . 0 4 4 4 1 0 0 4 3 . 2 2 8 . 5 1 2 0 5 1 . 6 3 8 4 L S D ( P = 0 . 0 5 ) 2 7 . 8 6 2 5 . 0 6 C V % 6 0 6 8 Qungjeuns,, *9l09,rflg>9, E R P IOL/_/5/«(6J5L_6OT Quntpn&a 0rjeuuiSlL@Lueb (PH = 1.5) Loss® P LOLLLD 0-15 QffiB ^,ipg0ei} ^djarflgggj. QuurflG9n9&D6sr G)«/7L©d5jD«J- 2.3.2. (§)© LilrjGLurra (j_pss>p9,eif\sstneb (eSag,EV. inesaessflp^en G9f[£9>6v) Qun&Guipmj P^ns/asis, 6nejleb iJIrjQujndltJi igneb ICXSIM/ gneurj P geiresiiDuSe^ILB sS\<3nsu99eOsyiLD sj puQiin euleswneu. eu6srsnrs§jeu GgnCun. snevl - (jjtssiensussig, G«^fy# 2025 (1994. leugH 6ULLL 3&ioj aj©L_ &1\ES)snffffeo ^ILL&IESIEM 18eb gnuLiiLQenensj. 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Qums,^ ajyjmrrsoriJD J25.9S2.50 12,350.00 18.060.00 ealnjU6S)6sruinSiu u4Sea>ev eui^LonbTsrib eJIpUbTaesTLurraluj afl/j) <9S6irvp/ euir^Lonesrib eiflpu&sieoTUjnSuj eulul Semen aen eutr^wnestLb ueoeSlear exuQtnnmw ©. 1.582.694.74 125.982.50 © . 12.350,00 18,060.00 1.739.087.24 182 flSlrflturraari) C&SDOJ (i) #hry<£WT6B«f)£ mjeanyuSlmfr SetaenoxaeniuLb Qunjjeijaiplgj r§6S)6vtug0p(gj 6U(g«D« grsQgnn - 742. &S)giuihi3XS>en QwajQanenens, Qarrrfliueun. unn6S>euuSlL.uuCL. )^gJ/<£S/TCBBf7«6T7 - 81 3>ULS,L0 114 (ii) uuSpol £jjpu LSIrjQgfLb &Lburf,&)LDrr&. ayeuip&s>em. GlarTQp^SlgQldjgeb, aeueurrgaj. iSestu. Qnyidi ^Sujmeu pplrbarresr ^fSeorroesiesr. - 45 pH urilQ&naymest 171 eSlrSQainSaouuCL. etfliSI amnjiaxv 35995 eiSlpues)6sriurTs]uj &5lul ajjemursioen 61750 urflCffrrsj6©OT«(5rF,u> i^sijsjrrtorr&ia^u) 01. aeueung,§j Smenaesien QeijLiL.ev 02. siggrfla QoijqM$Qg(jJigaiev 03. Qeuevlu uuM /^6M£/T6OT£ g/saw® 04. 4000 GignL-h (ipssien&j&sis, ^t&ian&sru gjesaQi 05. fpLLQgeb urflQ&rTg&n&iT 06. aioStmnesapn ayn&iof, §jem(Qi 07. QgufletaevuHev Garruul, tHenfg, nyQiems, 08. erebesnvtuleb 200 ajryeurra. a&srnjjaxii (LorripQiu uuSlfr Q&LU&BB;) 0 9 . {^(ip&nerreuesisjj urfXS&rra/m&sr 10. ueo <^55S7/_/5y«6rfl63r SGun&.«. ^nnuiff&u urflQorrgmm 183 THE TEA RESEARCH INSTITUTE OF SRI LANKA A N N U A L REPORT F O R THE Y E A R 1997 ISSN 1012 - 3954 Published by THE TEA RESEARCH INSTITUTE OF SRI LANKA ST. COOMBS, TALAWAKELE, SRI LANKA 1997 C O N T E N T S Report of the Chairman 1 Report of the Director 3 Report of the Administration Division 5 Report of the Finance Division 13 Research Projects Multi-disciplinary Research Projects Intercropping on tea lands to maximise income and conserve soil through optimal land utilization 15 Monodisciplinary Research Projects/Divisional Activities Agronomy 18 Biochemistry 51 Entomology 61 Plant Pathology 84 Plant Physiology 99 Plant Propagation and Breeding 105 Soils and Plant Nutrition 141 Technology 172 Statistics 192 Agricultural Economics Unit 193 St Coombs / St Lamiliere Estate 199 St Joachim Estate 207 Advisory and Extension Services Division 216 Low-country Station Research, Advisory and Extension Centre-Ratnapura 230 Mid-country Research, Advisory and Extension Centre-Kandy 235 Deniyaya Advisory and Extension Centre-Diyadawa 238 Uva Advisory and Extension Centre-Passara 241 Advisory and Extension Centre-Talgampola 244 Weather Tables Talawakele 246 Kandy 247 Ratnapura 248 Deniyaya - Diyadawa 249 Passara 250 Talgampola - Kottawa 251 Government Auditors Report 253 Report of the Chairman, Tea Research Board The action plan drawn up on restructuring of the Tea Research Institute (TRI) to meet the challenges of the 21st century was submitted to the Ministry for discussion at its Tea Sector Review meetings. A positive outcome of these discussions was the increase of the tea cess by 50 cts per Kilo of tea exported. The CADMAR (Composite Approach to Decision Making in Agricultural Research) process of research prioritization was carried forward to near completion. A cost-benefit analysis of all identified thrusts and projects, in both applied and basic research, is now in progress. Completion of this process will lead to maximization of resources, both human and financial, in the development of technology so needed for the tea industry to carry it through the next century and beyond. Renewed emphasis was placed in the resolution of legal matters of TRI with the assistance of the Attorney General and by obtaining his approval to retain private lawyers on a case by case basis. Matters receiving attention were in regard to defaulting of bonds by staff sent on postgraduate training abroad, defense of TRI in Labour Tribunal cases etc. All staff were required to submit reports within specified time limits on their return from of official visits abroad. They were also required to present debriefing seminars on their return for the benefit of other staff. The post of superintendent St. Coombs Estate was filled and a visiting agent was identified for the two TRI estates. The rationale being that these TRI estates should serve as model estates for the industry. The accounts unit at TRI Talawakelle was made responsible for the financial control of both TRI estates, a practice not followed in the past. The Director TRI was given "observer" status at meetings of the Sri Lanka Tea Board and was required to report on relevant matters to the Tea Research Board (TRB) Action was initiated to formulate a corporate plan for the TRI for the 5 year period beginning 1999. An internal committee with Director TRI as its Chairman was assigned this task. A qualified engineer was engaged m inspect and report on the vehicle fleet of TRI to facilitate replacement of those that are unserviceable. Inadequate transport facilities impede TRI's field programmes. A new generator was installed at the St. Joachim factory, replacing the old on which was beyond repair. This was done to circumvent losses in manufacture incurred due to constant power failures. Approval was given to TRI to produce video films on nursery practices, land selection and preparation and harvesting of tea and for training of its officers in this activity. These video films are for use both in estates and smallholdings in advisory and extension work. A protest was sent to the Ministry in regard to FAO supporting Sri Lankan participation 2 ANNUAL REPORT FOR 1997 at a conference abroad on tea bypassing TRI. Low interest loans were provided to TRI estate workers at St. Coombs Estate for electrification of their houses. A consultant was engaged to enable St Coombs Estate to obtain ISO 9000 series certification. Schemes of recruitment were drawn up for the posts of Computer Systems Manager and Resident Engineer. Approval was obtained from the Public Enterprises Division of the Ministry of Finance to create a cadre position for a Technical Editor for TRI. Till now this function has been performed by a senior scientist at TRI, an arrangement which needs correction due to the load of work involved. Approval was sought to create 10 posts of Heads of Divisions by suppressing an equal number of posts from the senior research officers cadre. Action was taken to extend the contractual period of Director TRI from I to 3 years and for revision of his emoluments. An application was made to patent the TRI plucking shear which has been adopted by a number of estates as well as small holders because of its superiority over manual plucking. TRI extension staff were permitted to work for plantation companies on payment without any deductions by TRI. It was decided that TRI should not maintain commercial tea nurseries, except at its Hantane station and St Jochim Estate, Ratnapura. In all other stations only model nurseries are to be maintained. The TRB approved the involvement of experienced scientists who had left the TRI and also university scientists m its research projects and for them to be sponsored by plantation management companies. Consequent to a decision to establish the Ceylon Tea Museum at Hantane Estate, Kandy the TRB approved a donation of Rs. 1 m. The Executive Director, Council for Agricultural Research Policy, Dr D Kirtisinghe and the Secretary General, the Planters' Association of Ceylon, Mr S K Seneviratne were appointed as members of the Consultative Committees on Research and Estates& Advisory Services, respectively. During the year the TRB held 09 meetings whilst its three consultative committees on Research, Estates & Advisory Services and Administration & Finance held 3, 3 and 8 meetings, respectively. Dr. S D I E Gunawardene Chairman, Tea Research Board. THE TEA RESEARCH INSTITUTE OF SRI LANKA ANNUAL REPORT, 1997 REVIEW OF THE DIRECTOR Arising from its responsibility as part of the agricultural research system of the nation, and towards fulfilling its mandate for research into the planting and manufacture of tea, the Tea Research Institute of Sri Lanka sets itself four­ square as a partner in the country's tea industry and specifically in the plantations and smallholder segments. Tea is a long-term perennial crop, and any progress in tea research is mostly subtle and cumulative rather than in the form of the proverbial breakthrough. The present Annual Report sets out details of research and extension activities for 1997 under divisional headings and, as with such Reports, it is necessarily a snapshot of activity during a single year. It is perhaps difficult, therefore, to peruse it and discern progress. For this reason, the Director's review is used to highlight achievements and advances rather than merely to abstract the details written by Divisions. Guidelines for intercropping tea with rubber were issued, based on on-going collaborative trials with the Rubber Research Institute of Sri Lanka. A shear harvester was developed and released for combatting the problems of low plucker-intake, labour shortage and unplucked green leaf during peak cropping seasons. It is light and manouverable and allows for leaf selectivity during harvesting. A patent was applied for together with a well- known company, Messrs RRR Jinadasa, who started mass-producing the shears for sale to growers. Growers began using them with excellent results. Soil conservation and improvement were advanced, biologically through the introduction both of an exotic species, Arachis pintoii, as a cover crop and of earthworms in vermicompost, and from the use of Flemingia congesta as hedgerow plants on sloping, marginal tea lands. A tea tissue-culture protocol was developed and collaboration was begun with a Sri Lankan company for testing its efficacy in large scale production of tea plantlets. Biochemical identification of tea clones was advanced through the development of an isozyme technique. Integrated pest management (IPM) and the de-emphasis of hazardous agro-chemicals were taken further. Guidelines were issued on the integrated 4 ANNUAL REPORT FOR 1997 management of shothole borer. The feasibility of controlling mite outbreaks using exotic predators was demonstrated, and a new, safe pesticide (a nitroguanidine) against scavenging termites, as efficacious as the chlorinated termiticides now on the banned list, was recommended. Soil surveys and GIS technology for maximising profitability from rational land use were applied to a further six marginal mid-country estates, following on the five completed in 1996. A rehabilitation plan for the tea industry in the mid-country was published. A smallholder Experiments and Extension (E & E) Forum was held for the first time ever, and in the Sinhala language, in December at the TRI, Ratnapura. This was deemed a great success. Two international meetings took place in Colombo during the year. The International Standards (ISO) Conference was held in February, with the TRI Director as leader of the Sri Lankan delegation. Issues of tea manufacture and packaging, and evaluation methods for obtaining data on which standards could be based, were discussed. A standard (ISO 11286) was proposed that required all teas to be graded on particle size. If adopted, this would have favoured CTC and not our variably-sized orthodox teas used in blending. We therefore put forward an escape clause, fortunately accepted unanimously, that "ISO 11286 shall not apply to blended teas". Other significant successes for Sri Lanka at the Conference included our obtaining from the Chair a clear and unambiguous statement that Sri Lankan teas were "the cleanest in the world", as far as pesticide residues were concerned. At least some of the credit for this happy state must go to the TRI. We were also able to allay, at this Conference, West European and German anxieties that our VP or clonal teas were the result of genetic manipulation. A Tea and Health Seminar was held in August. TRI research on estabUshing health benefits of tea drinking, using animal models, was presented. Dr W.W.D. Modder Director TEA RESEARCH BOARD OF SRI LANKA REPORT FOR THE YEAR 1997 1.1 Introduction The Tea Research Board of Sri Lanka was established on 12th November 1993 under the provisions of the Tea Research Board Act. No. 52 of 1993. According to the above Act, which came into operation on 07th March, 1994, the functions of the Tea Research Board shall be to engage in, and to encourage, foster and faciletate, research into the planting and manufacturing of tea. 1.2 The functions of the Tea Research Board The specific functions of the Tea Research Board are: (a) to conduct, assist and encourage, scientific and technological research into, and investigations of, all problems and matters affecting the production and manufacture of tea, including the prevention and control of pests affecting tea, the prevention and control of diseases affecting tea and the improvement of the quality of tea, as well as the diversification of products manufactured from tea; and to disseminate and publish at its discretion, the results of such research : (b) to conduct, assist and encourage, research into the economic viability of the tea industry in Sri Lanka including future economic trends in such industry; (c) to establish and maintain relations with research institutions in Sri Lanka and abroad; (d) to conduct, in the discharge of its functions, joint study programmes, seminars or symposia, with foreign research institutions and research institutions in Sri Lanka. 1.3 Tea Research Institute Head Office at Talawakeile The Head Office at Talalawakelle is responsible for the maintenance, administraion, overall planning and execution of research and extension and advisory programmes of its main centre at Talawakeile and five sub- stations located in different tea growing districts. 6 ANNUAL REPORT FOR 1997 1.4 Members of the Tea Research Board as at 31st December, 1997. 1. Dr. S.D.I.E.Gunawardhena Chairman 2. Dr. W.W.D. Modder Director, TRI 3. Mr. Rohan Illangaratne Member 4. Mr. Clifford Ratwatte Member 5. Mr.Anura Ekanayae Member 6. Mr.E Kanendran Member 7. Mr.S.K.Seneviratne Member 8. Mr.G.V.Tissera Member 9. Mr. J.P.M.Y. Ratnayake Member 10. Mr. Sivam Loganathan Member 11. Mr.V. Puthirasigamoney Member 12. Mr. I.L.A.Fernando Member 13. Mr.S.Wirasinghe Member 14. Prof. H.P.M.Gunasena Member Secretary to the Board : Mr. C.CMawilmada * M/S. G.V. Tissera and J.P.M.Y. Ratnayake served as the members of Tea Research Board until October, 1997. 1.5 Senior Management Staff as at 31st December, 1997 1. Director 2. Deputy Director Research ( Prod.) 3. Deputy Director Research (Tech.) - 4. Deputy Director (Administration) 5. Senior Accountant - Dr. W.W.D. Modder - Dr. G.D.Wimaladasa - Dr. M.T. Z. Mohamed - Mr.C. C .Mawilmada - Mr. W.B. Herath * Dr. G.D.Wimaladasa was terminated from the TRI service with effect from 19th May, 1997 on disciplinary grounds. 1.6 Heads of Divisions, Administration, Research & Advisory Staff - Grade I - V as at 31st December, 1997. Administration Division 01. Mr. C.CMawilmada 02. Mr.D.W.Bartholomeuz 03. Mrs.S.M.Jeyasinghem 04. Mr.D.S.E.Weerasuriya 05. Mr.B.Thilakeratne 06. Mr.M.m.P.J.Gawarammana 07. Mr.G.G.E.H.Gamage Deputy Director (Administration) Administrative Officer Secretary to the Director Chief Clerk Purchasing Officer Transport Officer Chief Motor Mechanic ANNUAL REPORT FOR 1997 7 08. Mr.U.A.Wickramasinghe 09. Mr.K.G.Piyasena 10. Mrs.S.Shanmuganathan 11. Mrs.P.Marapana 12. Mrs. Devika Rataayake 13. Miss. D.H.Kalikotuwe 14. Miss. A.P.V.Kalyani 15. Miss. C.S.K.Kiribathgoda 16. Mr.P.D.S.L.De Silva 17. Mrs.R.Godage 18. Mrs.I.Jayawickrama 19. Mrs.RJayasinghe 20. Miss. Ramani Wijeratne 21. Mrs. Chandrika Jayaram 22. Mr.Rajendrakumar 23. Mr.D.H.Jayathilake 24. Mr.S.H.Chandrasena 25. Mr.P.T.Perera 26. Mr.J.M.R.K.Bandara 27. Mr.R.W.Rengasamy 28. Mr.K.M.Seneviratne Banda 29. Miss. B.N.Damayanthi 30. Mr.W.P.A.N.Jayasinghe 31. Mr.V.Shanmuganathan 32. Mr.D.V.D.Vithanage 33. Mr.CJ.B.Abeykoon 34. Mr.W.C.K.Fernando 35. Mr.RD.Benadict de Silva 36. MrJ.G.Gamage Finance Division 01. Mr. W.B.Herath 02. Mr.G.A.K.P de Silva 03. Mr.M.Bowatte 04. Mr.K.D.H.Pathirana 05. Mr.T.H.Lokuhetti 06. Mr. S.G. Punchi Banda 07. Mr. C.B. Koswatte 08. Miss. D.M.R.Dissanayake 09. Mr.B.G.D. Premadasa 10. Mrs. N.Saparamadu 11. Miss. V.Pahalage 12. Mr.R.M.H.B.Ratnayake Electrical Foreman Stenographer (Sinhala) Stenographer (English) Stenographer (English) Stenographer (English) Stenographer (English) Stenographer (English) Stenographer (English) Clerk/Typist Clerk/Typist Clerk/Typist Clerk/Typist Clerk/Typist Clerk/Typist Clerk/Typist Clerk/Typist Clerk/Typist Clerk/Typist Electrician Electrician Telephone Operator Telephone Operator cum Receptionist General Clerk Clerk/Typist Work Supervisor Work Supervisor Chief Plumber Mechanic Plumber Mechanic Filter Plant Assistant Senior Accountant Accountant I Accountant U Chief Store Keeper Clerk/typist Accounting Assistant Accounting Assistant Accounting Assistant Clerk/ Typist Stenographer (English) Accounts Clerk Accounts Clerk 8 ANNUAL REPORT FOR 1997 13. Mrs. G.A.S. Gunasekara 14. Mrs. W.G.Piyaseeli 15. Mr. Saman Hewasilian 16. Mr.K.T.U. Kulathunge 17. Mr.W.A.Nishantha 18. Mr. M.W.T.P.De Silva 19. Mr.H.B.W. Gunasekara 20. Mr. H.B Thalghagoda 21. Miss. A.P.Amarathunga 22. Miss. P.V.D. Chandrakanthi 23. Miss. H.K.Seetha Accounts Clerk Accounts Clerk Accounts Clerk Assistant Store Keeper Data Entry Operator Assistant Store Keeper Store Assistant Accounts Clerk Accounts Clerk Accounts Clerk Accounts Clerk Internal Audit 01. Mr. R.Kariyawasam 02. Mr. P.S.Wickramasinghe Internal Auditor Internal Audit Clerk Library 01. Mrs. R.W.M.W.K. Illanganthilake 02. Mrs. R.W.M.S.K.Amunugama Publication Unit Librarian Library Assistant 01. Mrs. F.Y.M.Maharoof Publications/Publicity Officer Advisory & Extension Service Division 01. Mr.C.C.Rajasingham 02. Mr.J.C.K.Rajasinghe 03. Mr.B.A.D.Samansiri 04. Mr.V. Sidhakaran 05. Mr. K.R.M.Priyantha Agronomy Division 01. Dr. A. Kathiravettpillai 02. Mrs. M.S.D.L. De Silva 03. Mrs. J.Balasuriya 04. Mr. A.R.Amarasekara 05. Mr.U.P.Abeysekara Senior Advisory Officer Advisory Officer Advisory Officer Extension Officer Clerk/Typist Head of Division Research Officer Research Assistant Experimental Officer Experimental Officer ANNUAL REPORT FOR 1997 9 Agricultural Economics Unit 01. Mrs. J.A.A.M.Jayakody 02. Mr.T.P.B.Herath 03. Mr.G.Ganewatte Biochemistry Division 01. Dr. I. S. B. Abeysinghe 02. Dr. (Mrs) A.C.Liyanage 03. Mrs. JJayasundare 04. Mr. A.M.T.Amarakoon 05. Mr. P.B.Chandradasa 06. Mr. M.W.Silva Entomology Division 01. Mrs. S.LVitharana 02. Dr (Mrs) L.D.Amarasinghe 03. Mr. K. Thirugnanasuntharan 04. Mrs. K.M.S.M.Naghaulla 05. Mr.M.M.Keerthi Mohotti 06. Mr. D.D.Liyanage 07. Mr.N.Navaratne 08. Mrs. P.V.A.R.Abeysekara 09. Mr.P.D.Peter De Silva 10. Mr.G.P.Udumulla Plant Pathology Division 01. Mr.A.Balasuriya 02. Mrs. R.M.D.T. Pallemulla 03. Mr.T.S.Gunasekara 04. Mr.R.M.A.Rataayake 05. Mrs. K.M.N.K.Ratnamalala Plant Physiology Division 01. Dr. Ananthacoomaraswamy 02. Mrs.AJ.Mohotti 03. Mrs. V. Sidhakaran 04. Mrs.D.M.S.Nawaratne Senior Research Officer/OIC Research Assistant Research Assistant Actg.Head/Sen. Res. Officer Senior Research Officer Research Officer Research Assistant Technical Assistant Skilled Mechanic Actg.Head/Sen.Res. Officer Senior Research Officer Senior Research Officer Research Office Research Assistant Experimental Officer Experimental Officer Experimental Officer Technical Assistant Technical Assistant Research Assistant/Actg. OIC Research Officer Research Assistant Expermental Officer Experimental Officer Actg.Head/Sen.Res. Officer Research Assistant Technical Assistant Technical Assistant 10 ANNUAL REPORT FOR 1997 Plant Propagation & Breeding Division 01. Mr. V.Shanmugarajah 02. Mrs. M.T.K.Amarakoon 03. Mr. M.Ratnayake 04. Mr.S.W. Gunadasa 05. Mr. B.A.Ratnagoda Actg. OIC/Research Officer Research Assistant Experimental Officer Experimental Officer Technical Assistant Soil & Plant Nutrition Division 01. Dr. L.S.K.Hettiarachchi 02. Mrs. S.Ananthacoomaraswamy 03. Mr. G.P. Gunaratne 04. Mr. A.K.N.Zoysa 05. Mr. R.G.A.Wijewardhene 06. Mr. H.A.P.Warnasiri 07. Mr.S.M.Dissanayake Actg. Head/Sen.Res.Officer Research Officer Research Officer Research Assistant Experimental Officer Technical Assistant Technical Assistant Statistics Division 01. Miss. T.N.Senaratne Technology Division 01. Dr. M.T. Z. Mohamed 02. Mr.P.A.N.Punyasiri 03. Mr.W.S.Botheju 04. Mr. K.Raveendran 05. Mrs. S.H.P.Waduge 06. Mr. L. Jayasinghe 07. Mr.A.Nandasiri Research, Advisory & Extension Centres Data Entry Operator Deputy Director Resarch (Tech.) Research Officer Research Officer Chemical Engineer Experimental Officer Technical Assistant Workshop Mechanic (a) TRI Research Advisory & Extension Centre, Ratnapura (Low Country) 01. Mrs. S.LVitharana 02. Mr.S.Wimaladharma 03. Dr. M.A.Wijeratne 04. Mr.N.I.Giddawage 05. Mr.S.Koneswaramoorthy 06. Mr.G.L.C. Galahitiyawa 07. Dr. K.G.Premathilaka 08. Miss. S.M.Samarasinghe OIC/Actg.Head Entomology Actg. Head/Advisory & Exten. Senior Advisory Officer Senior Research Officer Administrative Officer Mechanical Engineer Research Officer Research Assistant Experimental Officer ANNUAL REPORT FOR 1997 11 09. Mr. H. S. N. Peiris 10. Mr. M. K. S. L. D. Amarathunga 11. Mr. A. K. Premathunga 12. Mr. E. R. Perera 13. Mr. C. Gunasekara 14. Mr. D. W. Vithana 15. Mr. A. K. M. Jayasena 16. Mr. K. T. C. Perera 17. Mr. K. D. Mervyn 18. Mrs. P. V. G. Karunanayake 20. Mr. J. S. K. De Silva 21. Mr. K. Gunawardhene 22. Mr. J. H. N. Piyasundera 23. Mr. E. W. D. P. Premathunga 24. Mrs. B.S. N. Vithana 25. Mr. R.Nadarajah 26. Miss. P.I. Jayawardena 27. Mr. M. A. B. De Silva Experimental Officer Extension Officer Experimental Officer Experimental Officer Experimental Officer Experimental Officer Technical Assistant Technical Asssistant Accounting Assistant Clerk/Typist Electrician Work Supervisor Technical Assistant Technical Assistant Technical Assistant Clerk/Typist Tel. Operator/Receptionist General Mechanic (b) TRI Research Advisory & Extension Centre, Hantane (Mid Country) 01. Mr. P. B. Ekanayake 02. Mr. S.T.Yatawatte 03. Dr. (Mrs) S. K. J. Liyanage 04. Mr. T. M. Sarathchandra 05. Mr. P. D. Uplai 06. Mr. H. J. M. De Silva 07. Mr. A. P. D. A. Jayasekara 08. Mr. U. B. Herath 09. Mrs. B. Sureshkumar 10. Mr. S. Wijethunga 11. Miss. R. M. S. Rajapakse 12. Mr. A. H. M. L. S. Abeysinghe 13. Mrs. U. Sritharan 14. Mrs. S. N. Wijesekara 15. Mr. H. Jayaweera 16. Mrs. K. Sarathchandra 17. Mrs. C. N. K. Edirisinghe 18. Mrs. B. K. S. Herath 19. Mr. L. Pahalamandiirige OlC/Senior Research Officer Advisory Officer Research Officer Experimental Officer Experimental Officer Experimental Officer Experimental Officer Experimental Officer Experimental Officer Experimental Officer Experimental Officer Experimental Officer Experimental Officer Experimental Officer Experimental Officer Technical Assistant Station Assistant Accounts Clerk Work Supervisor 12 ANNUAL REPORT FOR 1997 Advisory & Extension Centres (a) TRI Advisory & Extension Centre, Passara (Uva) 01. Mr. M. B. A. Perera 02. Mr. R. Nadasena OIC/ Advisory Officer Station Assistant (b) TRI Advisory & Extension Centre, Deniyaya 01. Mr. J. A. S. K. V. Jayasinghe 02. Mr. O. W. Jayawardene Actg. OIC/ Extension Officer Station Assistant (c) TRI Advisory & Extension Centre, Kottawa 01. Mr. K. D. Dhanayake 02. Mr. P. K. Jayawickrema 03. Mr. E. K. Somapala OIC/Advisory Officer Experimental Officer Station Assistant 1.7 Most of the vacant positions in the Administration inclusive of St. Coombs and St. Joachim Estates were filled during the year 1997 1.8 Action has been initiated to fill the vacancies in the Scientific divisions 1.9 Action has been initiated to provide electricity to workmen's cottages of St. Coombs Estate 1.10 Maintenance of Divisions : Engineering, Electrical and Water Supply of TRI continued to maintain a high standard in the maintenance of buildings, electrical installations and in the supply of water to the staff quarters within the TRI Campus during the year 1.11 Action has been taken to expand the security within the TRI Head Office and its Sub-stations 1.12 Action has been taken to renovate the Guest House at Head Office to a high standard 1.13 Action has been initiated to draft the Manual of Disciplinary Procedure of the Tea Research Institute 1.14 Action has been initiated to revise the existing schemes of recruitment's, promotions etc. 1.15 Action has been taken to obtain five (05) Nos. direct telephone lines through the WILLS telephone system. 1.16 The Tea Research Board held (09) nine meetings during the year 1997. The three Consultative Committees of the Tea Research Board on Research, Estate and Advisory Services, Administration & Finance held (03) three, (03) three and (10) ten meetings respectively. TEA RESEARCH BOARD BALANCE SHEET AS AT 31ST DECEMBER 1996 Tea Research Institute 1997 S i FIXED ASSETS Rs. cts. 240,485,117 Property, Plant, Equipment etc. 259,049,642.71 (135,108,291) Less: Accumulated Depreciation (Anx. I) ri45.S42.407.78) 105,376,827 113,507,234.93 9.817.789 Capital Work in Progress (Anx. II) 15.497.913.79 115,194,616 129,005,148.72 CURRENT ASSETS 9,487,822 Stocks (Anx. Ill) 8,188,402.65 64,197,010 Debtors and Other Debit Balances (Anx. IV) 86,887,766.01 11,892,067 Deposits, Pre-Payments & Purchase Advances (Anx. V) 12,753,162.33 8,531,632 Loans and Advances to Staff & employees (Anx. VI) 9,014,344.14 5,300 Other Current Assets-Patents 10,300.00 43,000,000 Short Term Investments-7 day Call Deposits 34,000,000,00 1.434.130 Cash and Bank Balances (Anx. VII) 10.237.544.92 138,547,961 161,091,520.05 337,400 Suspense (Anx. VDI) 169,285.48 344,051 Identified Losses (Anx. VOI) 500,557.87 18.713 Excess & Shortages (Anx. VIII) 14.319.21 139,248,125 161,775,682.61 CURRENT LIABILITIES (24,615,889) Creditors and Provisions (Anx. IX) (8,720,237.36) 114.632.236 Net Current Assets 153.055.445.25 229,826,852 Total Assets Less Current Liabilities 282,060,593.97 REPRESENTED BV 39,499,941 Grants and Reserves (Anx. X) 41,371,431.89 178,244,600 Tea Research Fund (Anx. XII) 213,441,709.80 - A/C Current St. Coombs Estate 721,887.13 - A/C Current St. Joachim Estate 13,514,155.15 754,670 Long Term Liabilities - Land Reform Commission 754,670.00 11,327.641 Provision for Gratuity 12.256.740.00 229,826,852 282.060.593.97 Note: - Negative figures are shown within brackets 1997 St. Coombs St. Joachim Estate Estate Total 1997 1997 1997 Rs. cts. Rs. cts Rs. cts. 259,049,642.71 z. i (145.542.407,78) 113,507,234.93 44.264.45 15.542.178.24 44,264.45 129,049,413.17 1,930,021.91 3,064,661.05 231,871.33 1,198,184.81 1.559.479.87 7,984,218.97 178,791.62 8,163,010.59 (5.572.412.11) 2.590.598.48 2.590.598.48 1,250,617.60 335,582.47 239,559.12 649,844.12 44.708.93 2,520,312.24 2,520,312.24 (14.530.167.51) (12.009.855.27) (11.965.590.82) 11,369,042.16 90,288,009.53 13,224,592.78 10,862,373.07 10,300.00 34,000,000.00 11.841.733.72 171,596,051.26 348,077.10 500,557.87 14.319.21 172,459,005.44 (28,822,816.98) 143.636.188.46 777.685.601.63 41,371,431.89 213,441,709.80 754,670.00 17 117.789.94 272.685.601.63 (721,887.13) - (13,514,155.15) 3.312.485.61 1.548.564.33 7^00,^08 48 (11,965,590.82) TEA RESEARCH BOARD TEA RESEARCH INSTITUTE OPERATING ACCOUNT FOR THE PERIOD 1ST JANUARY 1997 TO 31ST DECEMBER 1997 1996 Rs. 86,027,473 27,118,483 3,603,803 2,612,752 119,362,511 INCOME 3.1 Cess 3.2 Income from Estates and other commercial Activities (Anx XI) 3.3 Interst on Investments 3.4 Miscellaneous (Anx XI) TOTAL INCOME 1997 Rs. cts 94,433,902.31 33,910,939.53 3,226,181.53 4,279,303.85 135,850,327.22 EXPENDITURE 25,654,070 4.1 Personnel Emoluments 2,902,200 4.2 Travelling 8,924,263 4.3 Supplies and Requisites 8,805,953 4.4 Repairs and Maintenance of Capital Assets 8,896,949 4.4 Depreciation of Fixed Assets 13,159,130 4.5 Transportation, Communication, Utility and Other Services 4,976 4.5 Losses and Write-offs 1,191,516 4.7 Contributions, Grants and Subsidies 2,673,829 4.8 Pensions and Retirement Benefits 1,018,949 4.8 Gratuity Provision 437,180 4.10 Media, Advertising, Publicity and Gifts 4,050,270 4.11 Cultivation and Field Trials 5,250,791 4.12 Miscellaneous 82,970,076 TOTAL EXPENDITURE 36,392,435 Operating Srplus (1,311,017) Prior year adjustments 35,081,418 Excess of income over expenditure transferred to Tea Research Fund Note: Tea cess receivable - Rs. 58,427,836.69 Administration Advisory, Finance & Extensions Research Total Services & Publicity 12,117,232.40 5,743,082.00 13,260,524.79 31,120,839.19 2,134,248.35 1,107,862.00 1,043,393.83 4,285,504.18 1,520,491.50 2,473,927.00 5,481,648.84 9,476,067.34 9,250,027.17 2,371,841.87 1,390,186.68 13,012,055.72 3,120,430.76 2,184,611.44 2,995,255.21 8,300,297.41 11,367,986.35 1,967,218.00 2,410,912.43 15,746,116.78 29,237.67 - - 29,237.67 1,186,006.40 136,750.00 108,263.50 1,431,019.90 1,149,450.21 686,150.00 1,477,159.07 3,312,759.28 4,827,853.75 - - 4,827,853.75 76,398.00 387,254.00 10,477.68 474,129.68 4,328,304.61 - 4,328,304.61 498,518.59 323.442.00 3,374,577.63 4,196,538.22 47,277,881.15 21,710,442.92 31,552^99.66 100,540,723.73 35,309,603.49 (112,493.29) 35,197,110.20 a i o 3 JO V O RESEARCH PROJECTS The progress made in the multi-disciplinary and mono-disciplinary projects are reported below. 1. Project A/INCR - Intercropping tea lands to maximize income and conserve soil through optimal land utilization Project Leader - P.B. Ekanayake 1.1. Effect of intercropping tea and rubber on productivity 1.1.1 RRI-Kuruwita (1990) This experiment is conducted in collaboration with the Rubber Research Institute of Sri Lanka. Yields are presented in Table 1.1. Yield records were analysed separately for tea planted after rehabilitation and planted without rehabilitation. The latter was pruned for the second time in June 1997 and tipped in October, 1997. Results showed that the yield of monocropped tea was greater than intercropped tea, under rubber at spacings of 27'x8' or 40'x8\ but there was no significant difference in yield of tea between the two rubber spacings. The annual yield of tea in the mixed stand (tea +Rubber 40'x8') was 1086 kg ha/yr compared to the monocropped tea yield of 3852 kg ha/yr. Results on the pruning and tipping assessments were in agreement with the yield differences, i.e. although there were no significant differences, a slight reduction of the weight of prunings (15%) and tippings (4%) of tea was recorded under closer spacing of rubber compared with those under wider spacings. TABLE 1.1 Yield of tea under different spacing of rubber MULTI-DISCIPLINARY PROJECTS Green leaf Yield (kg bush1) Nov'96-Jun'97 Jul'97-Nov'97 With Rehabilitation Tea (monocropping) Tea+Rubber (8'x27) Tea+Rubber (8'x40') 0.654 0.318 0.323 0.894 0.270 0.300 LSD (p=0,05) 0.251 0.450 Without Rehabilitation Tea+Rubber (8'x27) Tea+Rubber (8'x.40') 0.378 0.430 LSD (p=0.05) NS 16 ANNUAL REPORT FOR 1997 1.1.2 St Joachim Estate, TRI-Ratnapura (1990) The yield is presented in Table 1.2. Although there was no significant yield difference (on a per bush basis) between monocropped tea and tea under rubber (40'x8') during the first half of the year, a significant reduction in tea yield was recorded under rubber during the second part of the year. The annual yield reduction of tea (per bush basis) amounted to be about 40%. Assuming a 70% stand of tea under rubber (at 40'x8'), made tea yield in the intercropped area (mixed stand) was estimated to be 1119 kg/ha/yr compared to the monocropped yield of 2635 kg/ha/yr. TABLE 1.2. Yield of tea under different spacing of rubber Green leaf Yield (kg bush1) Jan-June Jul-Dec Tea (monocropping) 0.352 0.627 Tea+Rubber (8'x40') 0.281 0.312 LSD (p=0.05) NS 0.303 1.13. Demonstration plot, St Joachim Estate, TRI-Ratnapura, (1989) Harvesting of rubber and tea continued together with other cultural practices. 1.1.4. Observation Block (1995) A second cut (45 cm) was given to tea plants and other cultural practices continued in this observation block. The same planting system was continued in another 2 ha block of the remaining area of this field. Tea and rubber clones planted were TRI 2027, RRI 100 and RRI 102. P.B. Ekanayake, M.A. Wijeratne and C. Gunasekara 1.2 Effect of intercropping in seedling tea 1.2.1 Effect of intercropping fruit species in seedling tea at Hantane Sub Station Except for mango all species had been damaged by wild boar. Therefore it was not possible to continue this observation trial and this experiment was abandoned. 1.3.Mid Country Station,Hantane - SALT Demonstration Hedgerows Four loppings were done during the year. Calliandra calothrysus, Cassia spectabilis and Flemingia congesta had given higher biomass production than other species. The biomass produced by Calliandra calothrysus was superior ANNUAL REPORT FOR 1997 17 while the biomass production of Desmodium rensonii, Adathoda visca and Teckoma starts was very poor. However, the low biomass production of Desmodium rensonii was attributable to the high casuality rate of this species with each lopping. The biomass production is presented in Table. 1.3 TABLE 1.3. Biomass production of different hedgerow species. Species Bio mass production (Kg ha') Calliandra calothyrsus Flemingia congesta Cassia spectabilis Adathoda vasica Teckoma stans Desmodium rensonii 6350 3874 3532 812 786 74 This demonstration trial is continuing. A.P.D.AJayasekera AGRONOMY DIVISION Acting Head - A. Anandacoomaraswamy 1. General Dr A. Kathiravetpillai retired from the services of the institute on July 9th after 35 years of service. Eh- K.G. Prematillake completed the Ph.D program at University of Reading and returned to the institute in June. Dr Kathiravetpillai attended a workshop on 'Organic Fanning' held in Calcutta in March. Mr H.S.N. Peiris spent 3 months at the Plantation Management Institute, Coonoor, South India. Mr.P.B. Ekanayake attended a workshop on 'Sustainable Farming systems' held in Japan. Ms Vanathy Yathavarajan and Anchana Balasingham, final year students of Faculty of Agriculture, University of Jaffna, Mr.T.D.P. Aratchige and Ms Dhammika Jayawardena from the Agricultural Faculty, University of Peradeniya carried out their final year projects. Patent right was obtained for the TRI shears and arrangements were made for its mass scale production by Messrs. P.P.P. Jinadasa. A first draft of a Monograph on Pruning was written by Dr. A.Kathiravetpillai and Dr. M.A. Wijeratne. A script for the video film on plucking was written by Dr.M.A. Wijeratne and Mr. Janaka Rajasinghe. Video filming on shear harvesting at Craig Estate was completed. 2. Project B/PLUK - Harvesting Practices Project Leader - M.A. Wijeratne 2.1 Effect of different methods of plucking (machine, shear and hand plucking) on yield of tea - Hapugastenna Estate, Ratnapura - (1992) Different combinations of machine, shear and hand plucking continued until the bushes were pruned in May 1997. Over the four months before pruning, yield of machine and shear harvested plots fell by about 48% and 34% respectively, compared to manual harvesting. The majority of the other plucking systems (combinations of mechanical and manual plucking) have given a yield loss of less than 10% compared to continuous manual plucking (Table 2.1). TABLE 2.1. - Yield of tea 4 months before pruning YieldMI'(kg/ha) Manual Plucking 1088 Machine plucking 565 Shear plucking 717 Manual + Machine (Cropping season) 1054 Manual + Shear (Cropping season) 1016 Manual + Machine (Alternate rounds) 995 Manual + Shear (Alternate rounds) 902 LSD (P=0.01) 186 ANNUAL REPORT FOR 1997 19 Plucking table heights of shear harvested bushes and manually harvested bushes were comparable while those of machine harvested bushes were 7-10 cm less than the manually harvested bushes. Pruning and tipping assessments showed no significant difference in weight of pruning and tipping between the plucking systems. However, there was a slight reduction of the pruning weight under machine harvesting. M.A. Wijeratne, D.W. Vithana and K.T.C. Perera 2.2 Mechanical harvesting at Rassagala Estate - (1996) Two plots of tea of about 0.5 ac (0.2 ha) each (TRI 2023) were harvested, one manually and the other by machine, to test the performance of the Kawasaki NV60H machine. In the field selected, manual plucking rounds of about 10- 12 days or more are usually adopted due to shortage of pluckers. Yields of both plots were comparable i.e. 2664 and 2617 kg/ha/year for the manual and machine harvesting. Output of the machine was estimated to be 250 kg/day (green leaf) and the labour output was 83 kg/man day. The machine can harvest about 0.53 ha/day. The selected field is on undulating land with an average slope. Further comparisons showed that the labour requirement for machine harvesting has been about 50% less than that for manual harvesting. M.A. Wijeratne, D.W. Vithana and K.T.C. Perera 2.3. Use of different kind of shears for plucking of tea. Field No. 3, St Coombs Estate (1996). Observation trial Five different shears viz. TRI shear, Malaysian shear, Shear with a bag, Shear with a box, Shear with a tray were used in comparison with manual plucking on six plots of tea as a demonstration. A.R. Amarasekara 2.4 Effect of Shear plucking on growth and yield of tea. Observation trial, Hantane Sub Station, Kandy (1997) Two blocks of approximately 0.1 ha are being continuously plucked by hand and TRI shear; the crop is recorded and a sample is separated into different units to ascertain dry weight. There was no significant difference between shear and hand plucking on yield recorded during the year, nor between other assessments made on dry weight of different units. S.N. Wijesekera 20 ANNUAL REPORT FOR 1997 3. Project A/PRUN - Pruning practices in tea Project Leader - M.A. Wijeratne 3.1. Study on different methods and time of pruning on the shot- ho le borer damage (SHB Task Force) at Kiriwanaganga Estate, Deniyaya (1996). Plots pruned in October were tipped and brought into plucking. The majority of recovering shoots were infested by shot-hole borer. The second set of plots (12 plots) were pruned in April, tipped and brought into plucking in October. The degree of shot-hole borer infestation of recovering shoots was less than the previous attack. (Complete assessments on the shot-hole borer infestation were done by the Entomology Division). M.A. Wijeratne, D.W.Vithana, K.T.C.Perera, and Staff of Entomology Division. 3.2. Study on different methods and time of pruning on the shot - ho le borer damage (SHB Task Force) at Attampitya Estate, Attampitiya (1996). An experiment was initiated to study the effect of different methods and time of pruning on the shot hole borer infestation. The operations carried out for different methods and time of pruning is given in Table 3.1. TABLE 3.1 - Methods and Time of Pruning and other operations Treatments Pruning Tipping Plucking 1. 10" Rejuvenation Oct. '96 Sept '97 Nov '97 2. 10" Rejuvenation Jan. '97 Sept'97 Nov '97 3. 10" Rejuvenation Apr. '97 - - 4. 18" Normal Oct. '96 Jan. '97 May '97 5. 18" Normal Jan. '96 Apr. '97 Aug '97 6. 18" Normal Apr. '96 Sep.'97 Nov '97 7. 24" Cut across Oct. '96 - May '97 8. 24" Cut across Jan. '97 - May '97 9. 24" Cut across Apr. '97 - Aug '97 Assessment of shot hole bore infestation was carried out by Entomology staff. A.R. Amarasekara ANNUAL REPORT FOR 1997 21 3.3 Effect of height of pruning, Type of pruning on yield and infestation of Shot Hole Borer, New Peacock Estate, Pussellawa (1997) This experiment was initiated to study the effect of different times and heights of pruning on the degree of shot-hole borer infestation and yield of tea. The Agronomy Division carried out pruning and will keep yield records while the Entomology Division will carry out other assessments on pest infestation. The treatments, method, height and time of pruning are given in Table 3.2. Table.3.2 - Type, height and time of pruning Treatments Type of Height of Time of pruning pruning pruning Tl Rejuvenation 25 cm April 1996 T2 -do- 25 cm October 1996 T3 -do- 25 cm January 1997 T4 Normal 45 cm April 1996 T5 -do- 45 cm October 1996 T6 -do- 45 cm January 1997 T7 Cut across 60 cm April 1996 T8 -do- 60 cm October 1996 T9 -do- 60 cm January 1997 Yield is being recorded in all the plots pruned in April 1996 and October pruning was also completed. The experiment is in progress. A.P.D.A. Jayasekera 3.3. Different styles of pruning and bringing into plucking on recovery after pruning and yield of tea at Noragalla Estate (1996). Analysis of results showed that there were no significant differences in the annual yield between the different methods of pruning (Rim-lung and cut- across) and bringing into plucking (tipping and plucking-in) tested. M.A. Wijeratne, D.W. Vithana and K.T.C. Perera 3.4. Seasonal variation of root starch reserves of tea with and without resting, St Joachim estate - Ratnapura (1996). The yield for the three clones and starch content are presented in Tables 3.3. and 3.4. f 22 ANNUAL REPORT FOR 1997 TRI 2027 4515 TRI 2025 3317 S 106 5249 LSD (p=0.05) 784 TABLE 3.4. Starch content of tea roots (%) Sample Number 8 10 12 14 16 18 Clone TRI 2027 2.1 4.0 9.1 10.6 8.7 7.9 TRI 2025 3.6 3.8 6.7 5.8 8.7 7.7 S 106 2.8 4.6 8.5 7.6 8.3 7.2 LSD (p=0.05) NS NS NS NS NS NS Although there was a significant difference in yield between clones, root starch content was not significantly different between clones. However, comparisons of monthly results revealed that starch reserves steadily increased with the expansion of the canopy foliage after tipping in October'96 and that it reached maximum about 6 months after tipping. Starch content appears to be constant during the following 6 months. Yield also varied in a pattern similar to that of starch content over the year. M.A. Wijeratne and P. Premathunga 3.5 Effect of height of pruning, retention of lungs and method of tipping on yield, St Coombs Estate, Talawakelle (1995) The yields during the 2nd year of the current cycle are given in Table 3.5 TABLE 3.5 - Effect of height of pruning, retention of lungs and method of tipping on yield Treatments Yield (kg made tea ha 1) Pruning at 45cm + with lungs + plucking in 3414 Pruning at 45cm + with lungs + tipping 2853 Pruning at 45cm + without lungs + plucking in 3016 Pruning at 45cm + without lungs + tipping 3298 Cut across at 55cm + with lungs + plucking in 3278 Cut across at 55cm + with lungs + tipping 3089 TABLE 3.3. Yield of made tea (kg ha'yr') Yield of made tea (kg fur' yr') ANNUAL REPORT FOR 1997 23 Cut across at 55cm + without lungs + plucking in 3208 Cut across at 55cm + without lungs + tipping 3414 Cut across at 65 cm + with lungs + plucking in 3176 Cut across at 65 cm + with lungs + tipping 2840 Cut across at 65 cm + without lungs + plucking in 2843 Cut across at 65 cm + without lungs + tipping 3332 LSD (P=0.05) NS CV% 9.4 Pruning heights, retention of lungs and method of bringing into bearing did not influence the yield. A.R. Amarasekara 4. Project B/ENGYII - Management of shade and fuelwood trees Project Leader : A. Anandacoomaraswamy 4.1 To evaluate the effect of Dadap shade on yield of tea - Field No. 7, Stonycliff Estate, Kotagala The yields for the second year (December 1996 - November '97) are given in Table 4.1. TABLE 4.1 - Effect of shade and no shade on yield Treatments Yield (kg MT ha 1) Without Dadaps 4562a With Dadaps 4400a SE 177.6 CV(%) 4.9 The treatment difference is not significant. A.R. Amarasekera 4.2.1 Evaluation of suitable Calliandra provenances Trials on Calliandra provenances at St. Coombs Estate, Talawakelle, Dessford Estate, Nanu Oya, and Park Estate, Kandapola are in progress. The total biomass (dry weight) obtained by the second lopping of branches in July at St Coombs trial is presented in Table 4.2. 24 ANNUAL REPORT FOR 1997 TABLE 4.2 - Total biomass (kg.ha1) Code Biomass (kg ha') 10/91/5 1655 10/91/19 1429 9/91/7 1339 10/91/16 1238 33/93/4 1153 33/93 1142 33/93/8 1112 9/91/6 1109 33/93/15 1087 10/91/20 1016 10/91/4 998 9/91/11 914 9/91/20 890 9/91/1 879 10/91/6 859 10/91 767 9/91/19 700 10/91/9 664 33/93/22 647 9/91/2 635 10/91/7 625 33/93/20 595 10/91/15 534 10/91/17 496 10/91/1 476 10/91/2 457 33/93/3 449 33/93/21 415 9/91/13 61 9/91/18 20 The biomass obtained by the first lopping at Park Estate, Kandapola is presented in Table 4.3. TABLE 4.3 - Total biomass (kg.ha1) Code Biomass (kg.ha') 4417 3075 2872 2776 10/91/1 9/91/13 9/91/20 10/91/7 ANNUAL REPORT FOR 1997 25 33/93 2294 10/91/5 2039 9/91/6 1873 33/93/15 1803 33/93/6 1748 33/93/10 1730 10/91/17 1540 33/93/22 1020 33/93/1 860 A.R. Amarasekara 4.2.2 Performance of different provenances of Calliandra, Mid Country Station, Hantane (1994) Calliandra plants were lopped in December. The percentage of surviving plants and the mean dry weight of lopping is given in Table.4.4 TABLE 4.4 - No. of plants and mean biomass production of different provenances of Calliandra Provenance Initial No. of plants % plants Mean dry wt of (Dec.1997) lopping (g/plant) 10/91 288 24.0 4968 34/93 515 39.5 4654 33/93 74 44.0 4611 9/91 543 20.0 4481 This trial is in progress A.P.D.A.Jayasekera 4.3 Evaluation of GreviUea robusta provenances About 30 provenances perforrriing well in the nursery were transplanted in the following estates in October/November for field testing: 1. New Peacock Estate(Pusellawa) 2. Pedro Estate(Nuwara Elliya) 3. St Joachim Estate (Ratnapura) 4. Galphele Estate(Panwila) A. Anadacoomaraswamy. K.G. Prematillaka, K.D. Dahanayake, A.R. Amarasekara and A.P.D.A. Jayasekara 26 ANNUAL REPORT FOR 1997 5. Project B/WATU Water use in tea plantations Project Leader: A. Anandacoomaraswamy 5.1 Soil reconditioning, soil fertility improvement and soil moisture conservation studies 5.1.1. Effect of soil rehabilitation with sugar cane varieties and grasses on the growth of tea. 5.1.1.1. St. Joachim Estate, Ratnapura - (1991) Yield records over the year showed that soil rehabilitation with different grass spp. and sugar cane cultivars before planting had no significant effect on the yield of tea. Different species tested were Sugar cane (CO 775, M 292/71, LF), Mana, Eragrostis and Vetiver. 5.1.2 Effect of rehabilitation after pruning on recovery and yield (K145). Observation trial, Mattakelle Estate, Talawakelle - (1991) The yield for the first year (November '96 - October '97) of the current cycle is given in Table 5.1 TABLE 5.1 - Yield of tea The plots were brought into plucking in November. Non- rehabilitated plots gave higher yield, probably due to their bottom slope position in the topography. 5.13. Effect of burying prunings on yield. St Coombs Estate (1991) This experiment is in the second year of the current cycle. The yields are given in Table 5.2. C. Gunasekara Yield (kg MT ha 1) With rehabilitation No rehabilitation 2236 2476 A.R. Amerasekera ANNUAL REPORT FOR 1997 27 TABLE 5.2 Effect of burying and retention ofprunings on yield Treatments Yield (kg MT ha 1) Tl . Control (with normal fertilizer) T3. Burying prunings + half of normal fertilizer T4. Burying prunings only T5. Burying brush wood + normal fertilizer T6. Burying brush wood + half of normal fertilizer T7. Retention of prunings + normal fertilizer LSD (P=0.05) CV% 4669 4167 3226 4242 3684 4510 567 9.1 There were no significant yield differences among treatments Tl , T2, T3, T5 and T7, but treatments T4 and T6 depressed the yield. 5.1.4. Effect of direct planting vs. planting after soil reconditioning on yield. Concordia Estate (1991) This experiment is in the fourth year of the first cycle. Yields are given in Table 5.3. TABLE 5.3 - Effect on yield of planting tea with and without rehabilitation Treatments Yield (kg MT ha 1) Clone Rehabilitated Direct planted Mean TRI 2025 1924 1739 1832 TRI 2024 2498 1835 2166 NAY 3 2850 2533 2692 DT 1 2798 2186 2492 PK 2 3170 2746 2958 Mean 2648 2208 2428 LSD (P=0.05) for planting 144 LSD (P=0.05) for clones 228 CV% 10.2 9.0 SE 246.8 208.5 All clones gave significantly higher yield in rehabilitated soil. 5.1.5 Effect of alternate methods of rehabilitation compared with rehabilitation under grass. New Peacock Estate.Pussellawa - (1996) The plants were tipped at a height of 65cm in October 1997. The tipping weight and girth at ground level are presented in Table 5.4 A.R. Amerasekera 28 ANNUAL REPORT FOR 1997 TABLE5.4- Tipping weightfkgha')andgirthfcm) Tipping weight Girth Control 216.0a 4.76a (2 years rehabilitation with grass) Direct planting with coir dust (3.0 kg/planting hole at bottom) 152.9ab 4.36ab Direct planting with compost (1.5 kg/planting hole + 20g T200 fertilizer mixture) 163.5ab 4.40ab Direct planting with coir dust (1.5 kg/planting hole at bottom + 1.5 kg compost on top) 135.2ac 3.96 ab Direct planting with coir dust (3.0 kg/planting hole at bottom + 20g T200 feitiiizer mixture) 108.9abc 4.43ab Direct planting with 20g T200 fertilizer mixture + 1.5 kg compost on top 84.7bc 4.26ab Direct planting with compost (3 kg/planting hole) 84.4bc 3.83ab mterplanting Flemingia congesta as cover crop in tea interrows 28.3c 3.1b LSD(P=0.05) 120 0.48 CV% 56.3 20.4 Tipping weights obtained from direct planting with compost and T200, direct planting with coir dust and compost and direct planting with coir dust and T200 were not significantly different from the control. A.R. Amerasekera 5.2 Cover crops Cover crops are maintained as a source of plant materials to be distributed among the plantations on request. M.A.Wijeratne, D.W.Vithana ANNUAL REPORT FOR 1997 29 5.3 Mulching materials 5.3.1. Effect of different mulching materials on growth and yield of tea, Galphele Estate, Panwila - (1993) Yields for the third year (January-December) are presented in Table 5.5. TABLE 5.5 - Effect of mulching materials on yield of young tea Treatments Yield(kg made tea ha 1) Tea refuse 3069 a Mana grass 3031 ab Paddy husk 2776 b Coir dust 2772 b Sawdust 2770 b Control 2571b SE 125.1 5%LSD 376.9 CV% 8.8 The plots mulched with tea refuse and mana grass gave higher yields than the control. This experiment is continuing. A.P.D.A. Jayasekera 6. Project B/WEED - Weed Management Practices in tea Project Leader K.G. Prematilaka 6.1 Studies on Passeli weeds in Bandarawela region. a) Preliminary investigations on the pattern of growth and reproduction of Passeli weeds in Neluwa, St. James and UvaHighlands estates. Table 6.1 gives morphological information on Passeli weeds. TABLE 6.1. Morphology of Passeli weeds in Uva Weed Taliniim paniculatum Andredera basseloides Bush type weed Vine type Plant density 80/m2 2-3/m2 Plant height 0.50 m 1.4-2.0 m No. of shoots/plant 3-10 6-7 Length of a branch 15-30 cm 75-90 cm 3 0 ANNUAL REPORT FOR 1997 No. of inflorescences/plant 3-10 No. of fruits/inflo. 25-100 No. of seeds/fruit 16-20 Total no. of seeds/plant 1200-15000 No. of bulbils/lm branch - 14-15 No. of bulbils/plant root - 150-200 b) Demonstration trials at Uva Highlands estate, Bandarawela. i) Testing of 'Bimaster' herbicide on the control of Passeli weeds. Herbicide was sprayed @ 4 1/ha in Sep.97, but no effective control of either weed was observed 2 and 4 weeks after spraying (WAS). ii) Use of lown-mower to slash Talinum weed. The weed could easily be crushed with the machine but it was difficult to move the machine on uneven land and the cutter blades were easily blocked by boulders. Continuous slashing was also hampered by the need to remove cut weeds which encircled both ends of the axle. These weeds have to be regularly removed from the axle for smooth running of the cutter. Some modifications will clearly be necessary for the machine. iii) Establishment of Arachis pintoii (a cover crop). This new cover crop was planted on vacant patches infested with Talinum weed in early October 1997. The cover crop failed 6 weeks after planting; replanting is to be done soon. c) Pot Experiments: i. Impact of the size of bulbils and shade on the germination of Passeli kodi (A. basselloides). Three sizes of bulbil were compared - Size 1 (0-1 g fresh weight) Size 2 (1-2 g fresh weight) Size 3 (2-3 g fresh weight) There were 3 reps arranged in a CRD under shade, and another 3 reps without shade. Treatments were applied in the open. 4 bulbils of each size were dibbled in the soil of each pot. Shade was provided by coir mats. Assessments: a) Date of emergence of first leaf. b) Rate of growth of seedlings. The experiment is in progress. ANNUAL REPORT FOR 1997 31 ii. Competitiveness of Passeli weeds (A. basseloides and T.paniculatum") on young tea under two light regimes. 30 cement pots of size 30x30x30 cm were filled with tea soils and planted with clone TRI 4042 in August 1997. Weed treatments were W 0 Control - no weeds planted W, T. paniculatum W 2 A. basseloides Half the experiment (15 pots as a CRD) was shaded (coir matting) and the other half unshaded. Two opposite corners of each weed pot (21.2 cm from the base of the tea plant) were planted with two plants of Talinum or Anredera weed according to the weed treatment. Assessments to be made: 1 Growth rate of tea - at two month intervals. a) Plant height b) Collar thickness c) No. of leaves d) No. of shoots 2) Growth rate of both weeds: a) Plant height b) No. of leaves c) No. of shoots plus, additionally, for Andredera d) No. of bulbils and e) Yam weight 3) Soil analyses prior to and 6 and 12 months after imposition of treatments 4) Plant analyses for N, P, and K, 6 and 12 months after imposition of treatments. 5) Ambient temperature under shade and no shade. K. G. Prematilaka and P. Prematunge. 6.2. Investigations on new "Control Droplet Applicator(CDA) sprayer" (supplied by Monsanto Co. Ltd). a) Spray Swath: I) Distribution pattern of the spray droplets within the ssath. While spraying with the CDA placing the hood at 20 cm height spray droplets were collected for a minute in small petri dishes. Dishes were placed 10 cm apart along a straight line from left to right and the spinning disc and hood were placed at the centre so that spray solution is collected in dishes. The details are given in Table 6.2. 32 ANNUAL REPORT FOR 1997 Table 6.2. Amount of spray solutions Distance from Shield level Shield parallel centre(cm) to the ground to the lance Left 40 1.5 0.2 30 2.2 1.2 20 2.9 3.5 10 3.6 4.8 Centre 3.2 5.3 10 1.95 1.75 20 0.4 0.1 30 0.1 0 40 0.0 0 Right The left side receives more droplets since the spinning disc rotates anti­ clockwise and thus spray droplets are released predominantly to the left direction. Positioning the hood and cup more towards the right makes an even distribution on both sides of the hood. ii) Spray swath as affected by the height of spinning disc from ground level. TABLE 6.3. Spray swath(cm) Height(cm) 15 22.5 30 Hood level to the ground 50 85 92 Hood raised upward by 30° 70 90 95 Hood bent downward by 30° 56 58 82 Holding the spinning disc at height between 22-30 cm while spraying is more appropriate to wet a wider area between tea rows with spray solution without harming tea bushes. b) Effect of walking speed while spraying with CDA and Knapsack sprayers on weed control one week after spraying(WAS). TABLE 6.4. % Weed control Sprayer Speed % Weed control grasses Broad leaf 1) CDA same as for Knapsack 90 45 (Normal) 2) CDA Slower than above 95 60 3) Knapsack Normal 50 10 ANNUAL REPORT FOR 1997 33 Thus, maintaining the speed for CDA similar to that for the Knapsack sprayer provides a satisfactory control of weeds. c) Investigation of the rainfastness of "Round up" sprayed once using a CDA sprayer. "Round up" was sprayed @ 1.651/ha using a CDA (the concentration was 66 ml/1 i.e. 1.65 1/25 1 of water/ha) and Knapsack sprayers (1.65 1/550 1 of water/ha) to the plots demarcated in open land infested with weeds. Following application, plots were sprayed with the same volume of water to wash away the chemical at given intervals according to the treatments. Treatments: Tl CDA - Watering 1 hr. after spray T2 do - Watering 2 hrs. after spray T3 do - Watering 3 2 hrs. after spray T4 do - Watering 4 2 hrs. after spray T5 Knapsack - Watering 4 2 hrs. after spray T6 CDA - Watering 5 2 hrs. after spray T7 Knapsack - Watering 5 2 hrs. after spray T8 CDA - Watering 6 2 hrs. after spray T9 Knapsack - Watering 6 2 hrs. after spray T10 Control - Visual observations were made 1,2 and 4 weeks after application of "Round up". TABLE 6.5 - Fresh weight of damaged weeds and visual observations. Fresh weight of damaged weeds 1 WAS(g/0.18 m2) 1WAS Tl 111 (1.96) ab 7.5 (2.80) T2 123 (2.05) a 10.0 (3.24) T3 55 (1.72) abc 10.0 (3.15) T4 123 (2.06) a 12.5 (3.59) T5 38 (1.58) c 2.5 (1.53) T6 95 (1.91) abc 20.0 (4.15) T7 48 (1.66) be 5.0(1.98) T8 100(1.99) ab 32.5 (5.53) T9 74(1.81) abc 7.5 (2.80) T10 59 (1.57) c 0.0 (0.71) LSD (P=0.05) NS Visual(% damage) 2WAS 4WAS 40 (6.36) abc 66 (8.16) ab 58 (7.60) abc 75 (8.50) ab 40 (6.40) abc 68 (8.24) ab 30 (5.45) abc 59 (7.56) b 25 (3.91) cde 12 (3.25) d 60 (7.76) ab 81 (9.00) ab 3 (1.53) ed 4(1.75) e 73 (8.48) a 90 (8.49) a 20 (4.53) bed 30(5.51) c 0 (0.71) e 0 (0.71) e WAS- Weeks after spraying Terms in parentheses = Log transformed values (weights) = Square root transformed values (%s) 34 ANNUAL REPORT FOR 1997 The greatest damage to weeds was observed throughout in plots watered 6 hour after spraying using the CDA sprayer. Howeve, greater damage was caused to weeds with CDA even one one hour after application of water than with the Knapsack sprayer. This might be due to the efficient use of glyphosate(1.65 1/25 1 water) with CDA compared to the Knapsack sprayer where 1.65 1 is mixed with 550 1 of water. With the Knapsack, four to five hours rainfastness resulted in lower weed damage, similar to that of the control. However, a six hour rain free situation showed a more weed damage with the Knapsack than with the control treatment. K.G. Prematilaka 6.3: Performance of Lawn Mower as a mechanical weeder. TABLE 6.6. Time taken to weed a 50 m interrow of tea. Lawn mower Knife Minutes Minutes Time - Worker A 34.0 Worker B - 23.0 28.6 23.0 Worker B 29.6 Worker A - 32.0 31.4 30.0 Average 30.7 27.0 Almost 90% of the weeds could be slashed with a knife but the lawn mowver was able to chop only a 60 % of the total weeds. Some modifications will clearly be necessary before operating the latter as a weeder. K.G. Prematilaka 6.4: Control of Borreria latifolia at the low country Station, TRI, Ratnapura. Treatments: Tl MCPA @ 1.91/ha (3.5 ml/1) T2 MCPA @ 2.51/ha (4.5 ml/1) T3 MCPA @ 3.01/ha (5.5 ml/1) T4 MCPA @ 1.91/ha (3.5 ml/1) + Paraquat @ 1.11/ha (2 ml/1) T5 MCPA @ 2.5 1/ha (4.5 ml/1) + Paraquat @ 1.11/ha (2 ml/1) T6 MCPA @ 3.01/ha (5.5 ml/1) + Paraquat @ 1.11/ha (2 ml/1) T7 Hedonal-D @ 1.41/ha (2.5 ml/1) + Paraquat @ 1.11/ha (2 ml/1) T8 Hedonal-D @ 1.91/ha (3.5 ml/1) + Paraquat @ 1.11/ha (2 ml/1) T9 Hedonal-D @ 2.5 1/ha (4.5 ml/1) + Paraquat @ 1.11/ha (2 ml/1) T10 Control - no herbicide ANNUAL REPORT FOR 1997 35 The above herbicide combinations were applied to the tender patches of Borreria in early October, 97, with three replicates. Observations were made 7,14 and 21 days after spraying. TABLE 6.7.Mean visual score(%) for casualties 1, 2 and 3 weeks after spray (WAS): 1 WAS 2 WAS 3 WAS Tl 50 50 85 T2 75 100 100 T3 82 100 100 T4 50 98 100 T5 65 85 92 T6 85 83 98 T7 82 90 92 T8 98 100 100 T9 10 100 100 T10 0 0 0 It appeared that 2.5 l/ha of MCPA alone or 1.9 l/ha + paraquat 1.1l/ha and 1.91/ha Hedonal-D + 1.11/ha paraquat were more promising on the control of Borreria latifolia weed at its immature stage of growth. However, MCPA @ 1.9 1/ha alone and Hedonal-D @ 1.4 l/ha also killed the weed satisfactorily 3 WAS. K. G. Prematilaka 6.5 Studies on the efficacy of glyphosate with incorporation of kaolin in the control of Axonopus grass at, St Joachim Estate. 36 blocks of size 2 m2, which were highly infested with Axonopus compressus grass, were used for three replicates of the following twelve herbicide treatments. Tl 2% glyphosate alone T2 1% glyphosate + 3.85 kg/ha kaolin T3 1% glyphosate + 1.93 kg/ha kaolin T4 1% glyphosate alone T5 0.5% glyphosate +3.85 kg/ha kaolin T6 0.5% glyphosate + 1.93 kg/ha kaolin T7 0.5% glyphosate alone T8 0.3% glyphosate + 3.85 kg/ha kaolin T9 0.3% glyphosate + 1.93 kg/ha kaolin T10 0.3% glyphosate + 0.97 kg/ha kaolin Ti l 0.3% glyphosate alone T12 Control - nil 36 ANNUAL REPORT FOR 1997 Visual observations as well as weed fresh weight differences did not indicate any effect of kaolin on weed damage. K.G. Prematilaka 6.6 Effect of different methods of weed management on yield of young VP tea, Galphele Estate, Panwila (1994) The yields during thr 3rd year of the first cycle are presented in Table 6.8 Table.6.8 - Effect of method of weed management on yield of young VP tea Treatments No.of weeding Yield rounds in 1 yr. (kg ha1) Manual weeding every month 12 4456 Manual weeding every 2 months 6 4056 Manual weeding every 3 months 4 4196 Chemical weeding with Paraquat 4 3896 Chemical weeding with Glyphosate 3 4123 Chemical weeding with Sulphosate 3 3834 Chemical weeding with Paraquat + 24D 2 4159 Chemical weeding with Paraquat+24D + Diuron 2 4184 Slash weeding 3 4506 SE(16df) 262.0 CV% 6.9 The results indicate that slash weeding and monthly manual weeding gave significantly higher yield than other treatments, while Paraquat and Sulphosate led to lower yields. This experiment is to continue. A.P.D.A. Jayasekera 6.7 Effect of different frequencies of paraquat application on yield of tea compared to manual weeding, St Coombs Estate, Field No. 8 (1995) Treatments continued during the year. The plots were in the third year of the current cycle and the yield data is presented in Table 6.9 ANNUAL REPORT FOR 1997 37 TABLE 6.9. Yield of tea Treatment Yield (kg made tea ha-) Tl - Manual weeding (monthly interval) T2 - Paraquat at 1.11 ha"1 (monthly) T3 - Paraquat at 1.11 ha 1 (every 2 months) T4 - Paraquat at 1.11 ha 1 (every 3 months) LSD (at P=0.05) CV% 1695 1614 1667 1901 NS 5.6 No significant differences in yield were observed. This experiment continues. 6.8 Effect of different weed populations on yield of mature tea, St Coombs Estate, Field No. 7 Different levels of weed populations (obtained by removal of weeds) such as 15%, 30%, 45% and 60% were compared with control plots (no weeding) and the yield of tea is presented in Table.6.10 TABLE 6.10. Yield under different weed populations Treatment Yield (Made Tea kg hal) 15% weed population 1679 30% weed population 1652 45% weed population 1547 60% weed population 1636 Control (80-90%) weed population 1592 CV% 10 LSD (at P=0.05) NS There was no significant yield reduction in the 2nd year of the 3rd cycle, probably due to lack of competition between the crop and the weeds. This experiment is continuing. 6.9 Control of problem weeds 6.9.1 Effect of Anredera basseloides on yield of tea. St Coombs Estate, Fields 5 and 9. M.S.D.L. de Silva M.S.D.L. de Silva An experiment was initiated to study the effect of Anredera basseloides on yield of tea. The plots were pruned in September 1996, and treatments 38 ANNUAL REPORT FOR 1997 were imposed in April 1997. The yield for the following 8 months is presented in Table 6.11 TABLE 6.11 Yield of Tea under various control measures Treatments Yield (kg ha 1) Field 5 Field 9 1. Uprooting of weeds with yams every fortnight 3468 2509 2. Slashing of weed to ground level every fortnight 3233 2261 3. Tipping the weeds at the canopy height of tea at weekly intervals 3242 2467 LSD(P=0.05) NS NS CV% 11 17 M.S.D.L. de Silva 6.10. Weed survey on Anredera basseloides conducted by a questionnaire. In the Up-country, 58 out of 143 estates responded to the questionnaire. In Uva only 21 out of 67 estates responded. In the Up-country, 34 estates were infested with Anredera basseloides. The degree of infestation ranged from 1-30%. In Uva, 17 estates were infested, with infestation from 1-40%. M.S.D.L. de Silva. 6.11. Study on the development of resistance in Erigeron sumatrensis (retz) to 'Paraquat' in Up-country. Paraquat was applied at 4 different rates (0,0.165,0.26,0.325 and 0.39 a.i kg ha 1) to naturally grown Erigeron populations at 4 growth stages (2-4, 5-6, and 7-8 leaf stages, and flowering stage). The impact of herbicide was estimated by the efficiency of Photosy stem U(Fv/Fm) and leaf cell membrane permeability. The results indicated that Erigeron has developed resistance to all the levels of paraquat tested and at all growth stages. However, it has not developed cross- resistance. Erigeron could be controlled by Glufosinate ammonium which has a similar mode of action to paraquat. The other herbicides - Glyphosate, Metribuzine and Hedonol (2,4 D + MCPA) also killed the weed successfully. M.G.D.L.Priyantha, M.S.D.L.de Silva, A.Anandacoomaraswamy and B.Marambe ANNUAL REPORT FOR 1997 39 6.12 The following weed specimens were added to the herbarium at St. Coombs: Eragrostis nigra (Graminae) Cyperus melansperus (nees) Suringer (Cypereceae) Eragrostis tennifolia (Graminae) Cyperus compressus L (Cypereceae) Cyperus distans L.F (Cypereceae) Elusine indica (L) Gaerta (Graminae) Cynodon arcuatus J.S.Prest (Graminae) Hewittia sublobata (Con volvulaceae) Salvia tiliifolia (Labiata) Clidemia hirta (Melastomataceae) Stachytarpheta urticaefolia (Verbemaceae) Aster amellus (Compositae) Wikstroemia indica (Thymelaeaceae) Ceropegia candilabrum (Acletiadaceae) M.S.D.L. de Silva, S.N. Wrjesekara and R.S.S. Rajapakse 7. D/AGRY - Divisional Activities 7.1. Growth Regulators 7.1.1 Foliar Application of Vegemax on tea (Observation Trial). Mature tea was first sprayed with Vegemax @ 5 ml/201 of water on 30th July '97, this being repeated on 12th August and 25th August. Yield was then recorded over a 3-month period. Prior to the application of treatment pre- harvest yield was recorded for three rounds of plucking. The yield is presented in Table 7.1 TABLE 7.1 - Effect of Vegemax on yield of tea Mean tea flush yield(g/plot/round) Date of spray Vegemax Control Pre-treatment(July) 367.5 391.0 Post-treatment Aug. 246.5 214.0 Sep. 285.8 257.5 Oct. 230.5 237.3 Foliar application of Vegemax gave no significant improvement in yield. K.G.Premathilaka 40 ANNUAL REPORT FOR 1997 7.1.2. Effect of spraying Cytozyme (growth regulator) on yield of mature tea, Clone TRI 2023 - Galphele Estate, Panwila (1997) A study was initiated to test the effect of spraying a growth regulator (Cytozyme) on the yield of mature tea (TRI 2023). Seven applications were given and the yield obtained is presented in Table 7.2. TABLE 7.2 - Effect of spraying Cytozyme on Yield Treatments Control Cytozyme at 0.51 ha"1 Cytozyme at 1.01 ha"1 SE LSD(P=0.05) CV(10%) Yield(kgMT/ha) 4460 5002 5448 247.1 855 9.9 A.P.D.A. Jayasekara 7.2. Effect of different size of polythene bags on growth of cuttings in tea nurseries (1996) Measssurements of growth on clones TRI 2025, TRI 2027, TRI 3055 and TRI 4061 over a period of 10 months showed that number of leaves, shoot weight and root weight all increased with larger polythene bags (Table 7.3). The largest size polythene bag (6"x9") produced the biggest nursery plant. However, the weight gain of the 6" bag over the 5" bag was less than that between 5" and 3". Of the clones tested, growth of TRI 3055 plants was faster than all other clones and TRI 2027 recorded the slowest rate of growth in the nursery. Owing to the higher percentage of casualties after a dry spell, this experiment was repeated in the St. Joachim estate nursery. TABLE 7.3 - Growth of nursery plants 8 month 10 month Leaf shoot Root Leaf shoot Root no. wt. wt. no. wt. wt. Clone TRI2025 12.4 3.0 0.68 14.4 4.8 1.5 TRI2027 12.1 1.3 0.29 16.8 3.6 0.7 TRI3055 12.4 3.3 0.87 25.0 7.6 2.2 TRI4061 17.2 2.4 0.42 21.4 5.7 1.4 LSD (p=0.01) 4.21 0.82 0.23 3.85 1.63 0.46 ANNUAL REPORT FOR 1997 41 Bag diameter LSD (p=0.01) 3" 5" 6" 10.3 14.6 15.6 2.73 1.4 2.6 3.5 0.52 0.32 0.59 0.79 0.15 11.3 20.6 26.3 3.34 2.2 5.9 8.2 1.41 0.9 1.4 2.1 0.40 M.A Wijeratne and P. Premathunga 7.3. Studies on the drought tolerant properties of tea clones. Studies were conducted in collaboration with the Faculty of Agriculture, University of Peradeniya. Results of the glasshouse experiments showed that moisture stress increased diffusive resistance, thereby reducing water potential and relative water content of tea leaves. Wider osmotic adjustments and lower water potential at zero turgor were recorded in most TRI 4000 series clones than in the control (TRI 2025) and TRI 3000 series clones. The variations of these water relation characteristics of the latter two clones were roughly similar. Wider osmotic adjustments and lower water potential at turgor loss point of TRI 4000 series clones suggest that they are more drought-tolerant than the other clones tested. 7.4. Effect of bud breaking agent Dormex on yield of tea. The details of the series of trial were reported in the annual report 1996. These trials were continued into the 2nd year in order to determine whether there is any depression of yield following the boost in yield obtained in the first year. St Coombs Estate, Field no 7 The yield obtained for the period November 1996 - June 1997 is given in Table 7.4. This trial has now been terminated, after 8 months. TABLE 7.4 - Effect of Dormex on yield of tea Treatments Yield (kg ha 1) M. A. Wijeratne, Asha Karunaratne TRI 2025 DTI Control for DOP jg Sprayed on DOP @ 1% 1 7 3 1 Sprayed on DOP @ 2% 1680 Control for 3DAP j 7 0 5 Sprayed 3 DAP @ 1% 1 7 6 7 Sprayed 3 DAP @ 2% 1 7 1 ] LSD (P=0.05) N S 1769 1801 1833 1829 1936 1893 NS (Note: DOP = Day of Pruning, DAP = Days after Pruning) Treatment differences were not significant. 42 ANNUAL REPORT FOR 1997 1. Hantane Estate, Mid-country, Kandy Field no 7. Factory division seedling tea 2. Galphele Estate, Panwila, Field No 9A TRI 2023 The yields obtained for the period October 1996 - August 1997 are given in Table 7.5. TABLE 7.5 - Effect ofDormex on yield of tea Treatments Yield (kg ha 1) Hantane Galphele Estate Estate Control for DOP 698(b?) 1627a Sprayed on DOP @ 1% 793a 1827a Sprayed on DOP @ 2% 780a 1870a Control for 7DAP 643a 1717a Sprayed 7 DAP @ 1% 756a 2025a Sprayed 7 DAP @ 2% 677ab 1728a A.Kathiravetpillai, A.P.D.A. Jayasekera and S.N. Wijesekara Low Country, Galle- TRI Substation, Talgampolla, Field No 7. The yield obtained during the eight months of the second year from November 1996 to June 1997 is given in Table 7.6 TABLE 7.6 - Effect ofDormex on yield of tea Treatments Control for DOP Sprayed on DOP @ 1% Sprayed on DOP @ 2% Control for 7DAP Sprayed 7 DAP @ 1% Sprayed 7 DAP @ 2% LSD(P=0.05) Yield (kg ha 1) 2897 2933 2981 3005 3100 3235 NS There were no significant differences in yield between any of the treatments. The trial has been terminated. A.Kathiravetpillai and RKJayawickrama ANNUAL REPORT FOR 1997 43 Other trials conducted to study the effect of Dormex on yield of tea. Gonakele Estate, Passara, Passara division, Field No VP9. This study was conducted with clone TRI 2027 to investigate the effect of spraying Dormex using a Knapsack sprayer and a Mist Blower. The design was of the split-plot type with 3 replicates of main treatments (0,1% and 2% Dormex) x (times of application), split for sprayer used, 36 plots in all with 60 bushes per plot. The tea was pruned on 27th Octber 1995 and spraying was carried out on (1) day of pruning (DOP) and (2) 7 days after pruning (DAP). There were no significant differences between the treatments over a period of one year from March 1996 to February 1997. The yields obtained are presented in Table 7.7. TABLE 7.7 - Effect of Dormex sprayed by knapsack sprayer and Mist blower on yield of tea Treatments Yield (kg Fwt plot1) Knapsack Mist Blower sprayer Control for DOP 376.4 322 8 Sprayed on DOP @ 1% 3719 355 9 Sprayed on DOP @ 2% 424.3 347.3 Control for 7 DAP 34O.5 366.5 Sprayed 7 DAP @ 1% 325.8 373.1 Sprayed 7 DAP @ 2% 350.8 380 6 LSD(P=0.05) NS NS There were no significant differences in yield between the treatments Norogolla Estate, Ratnapura Clone TRI 2023. Spraying was carried out using a knapsack sprayer and a mist blower on blocks of 0.5 acre (0.2 ha) each. Pruning was done on 2nd July 1996 and spraying was carried out on (1) day of pnining and (2) 7 days after pruning at 1% concentration. The yield of tea (kg fresh weight per plot) obtained from October 1996 to June 1997 is given in Table 7.8 44 ANNUAL REPORT FOR 1997 TABLE 7.8 - Yield of tea Treatments Yield (kg Fwt plot1) Knapsack sprayer Mist Blower Control for DOP Sprayed on DOP @ 1% Sprayed on DOP @ 2% 1993 1662 2242 1456 1181 1653 Spraying 7 days after pruning improved the yields by 12-13%. D.W.Withana and M.W.Wijeratne Observation trial at Dessford Estate, Nanuoya The trial was carried out on clonal tea in 2 blocks of 100 bushes each. The tea was pruned in June 1995 and Dormex was sprayed on one block 1%, 7 days after pruning. The yield kg fresh weight for the period November 1995 to October 1996 is given in Table Table 7.9. TABLE 7.9 - Yield of tea with and without Dormex Treatments Yield(kg MT ha 1) Control 1861 Sprayed 2187 7.5 Earthworm culture 7.5.1 Effect of inoculation of earthworms on yield of tea. Galphele Estate, Panwila (1995) The yield recorded in the 3rd year after the treatment application is presented in Table 7.10 TABLE 7.10 - Effect of inoculation of earthworms on yield of tea Treatments Mean yield (kgMT ha-i) T.750 + 600 earthworms 1929 T.750 alone 1683 The results indicated that inoculation with earthworms enhanced the yield significantly (t=2.3152*). The experiment continues. A.Kathiravetpillai ANNUAL REPORT FOR 1997 45 7.5.2 Effect of earthworms casts on growth of young tea plants, Hantane Sub Station (1996) The growth measurements made 18 months after planting are presented in Tables.7.11 and 7.12 TABLE 7.11 - Effect of earthworm casts on growth of tea Treatments Shoots Leaves Roots No. DW(g) No. DW(g) No. DW(g) 75g casts 15b 8.7b 119b 35.5b 13b 15.5b 150g casts 15b 21.3b 144b 45.0b 14b 29.7ab 250g casts 24a 40.8a 243a 82.3a 34a 56.8a T.200 16b 19.2b 152b 34.4b 15b 47. lab SE ? ? ? ? ? ? % c v 18.8 51.5 34.1 42.3 16.3 55.4 TABLE 7.12- Effect of earthworm casts on growth of tea Treatment Dry wt. of main stem Total dry wt. Casts 75 g 29.17b 138.35b Casts 150 g 46.30ab 206.19ab Casts 250 g 79.70a 297.44a T.200 30.91b 180.70ab % CV 34.20 25.15 The results indicate that plants treated with 250 g earthworm casts had exhibited enhanced growth compared to other treatments. This experiment has been terminated. 7.5.3 Effect of inoculation of earthworms on growth of tea plants, Hantane Sub Station, Kandy This experiment was carried out in lm x lm x 3 m cement tanks. Treatments: Tl - Inoculation of 100 earthworms per tank + tea fertilizer at recommended rate T2 - Tea fertilizer at the recommended rate Design: Paired comparison with 5 replicates 46 ANNUAL REPORT FOR 1997 The results of the second destructive sampling is presented in Table.7.13 TABLE 7.13 - Effect of inoculation of earthworms on growth of tea Measurement Earthworm Fertilizer tcalc + Fertilizer only LNo.of roots 17.8 17.8 0.50 2.No.of leaves 117.0 158.0 0.74 3.No.of branches 13.4 14.8 0.50 4.Dry wt of roots(g) 24.5 31.93 0.55 5.Dry wt of leaves (g) 35.1 53.92 1.00 6.Dry wt of side shoots(g) 18.3 27.27 0.80 7.Dry wt of main stem(g) 37.5 53.92 0.81 8.Total dry wt(g) 163.9 244.88 0.87 There were no significant differences between any of the treatments. The experiment continues. 7.1.4 Effect of inoculation of earthworms with and without compost on yield of tea, Aningkanda Estate,Deniyaya. The yields recorded during the first year after treatment application are presented in Table.7.14 TABLE 7.14 - Effect of earthworm with and without compost on yield of tea Treatments Tield(kg ha 1) Tl Earthworm+Mana+Compost+Tea fertilizer 2849a T2 Earthworm+Compost+Tea fertilizer 1838b T3 Earthworm+Mana+Compost 1783b T4 Earthworm+Mana+Tea fertilizer 2425ab T5 Earthworm+Mana 1882b T6 Mana+Compost+Tea fertilizer 2146ab T7 Compost+Tea fertilizer 1593b T8 Mana+Compost 2282ab T9 Mana+Tea fertilizer 2341ab T10 Tea fertilizer alone 2403ab CV 25.8 Compost - 8000 kg ha 1, yr 1 and tea fertilizer at recommended rates. The results indicate that there was a higher yield in treatment Tl and the increase in yield was significant compared to the treatments T2, T3, T5 and T7. The experiment is in progress. ANNUAL REPORT FOR 1997 47 250g casts + T 200 at recommended rate 150.26ab 250g casts + T 200 at half recommended rate 182.99a 250g casts only 131.03b T 200 at recommended rate 124.68b % CV 19.2 The results indicate that there was a significant increase in yield with earthworm casts + T 200 (either rate) when compared to either alone. 7.5.5 Effect of Vermi compost on growth and yield of tea, Doragal, Kotmale (Observation trial) This observation trial was initiated to investigate the effect of Vermi compost on soil fertility improvement and growth and yield of tea in a tea small holding located in Upper Mahaweli catchment area. The Small holder was trained to culture the earthworms and produce Vermi compost using cow dung, kitchen waste and mana grass. The land was divided into 2 parts. Treatments: Tl 250g Vermi compost per plant T2 T 200 fertilizer at recommended rate Six applications were given after April, and visual assessment indicated enhanced growth on plants treated with Vermi compost. The experiment is in progress. R.M.S.S. Rajapakse 7.5.4 Effect of earthworm casts on growth and yield of tea, Galphele Estate, Panwila Treatments: T1-250 g casts per plant + fertilizer at recommended rate T2-250 g casts per plant + fertilizer at 2 recommended rate T3-250 g casts per plant T4-T 200 fertilizer at recommended rate The experiment was initiated in June on plants given the first cut at 15 cm by the estate. After the treatment application plants were given second and third cuts at 30 and 40 cm and tipped at 50 cm before plucking commenced in December. The tipping weight is presented Table 7.15. TABLE 7.15 - Effect of earthworm casts on growth of tea Treatments Tipping weight(kg ha-1) 48 ANNUAL REPORT FOR 1997 7.6 Determination of optimum plot size for experimentation and effect of plucking table variables on yield An investigation was carried out to determine the optimum plot size for experimentation in tea and to identify the possible relationship between plucking table variables (area of plucking table and active:banji ratio) and yield. TRI 2025 planted at a spacing of 120cm x 60cm was used at low (Ratnapura), mid (Hantana) and high (Talawakele) elevations for this study. Fairfield Smith's (1938) procedure and analysis of varience were used. It was found that the optimum number of bushes per plot should be between 10-12, at all three sites. This also confirmed the earlier results obtained by Fairfield Smith. A multiple regression using the standardised values (z values) of plucking table variables showed that only plucking table area was weakly correlated with yield at all locations. There was no correlation between active:banji ratio and yield. T.D.R Aratchige, R.O. Thatill, RB. Ekanayake, M.A. Wijeratne and A. Anandacoomaraswamy 9. Particulars regarding seminars/conferences attended/participated and publications published/submitted a) P.B.Ekanayake 1) Served as a member of the cornmittee on "Rehabilitation of the Tea industry in Mid Country" 2) Participated in Group discussion held at Talawakelle on "SALT and Covercrops" in March organized by RSC 1 3) Participated and presented a paper on "Soil management in tea plantations" at the Workshop on Watershed Management held at Nuwara Eliya from 24th to 25th April 4) Addressed the Superintendents of Kahawatte Plantations Ltd., on "Covercrops in tea lands" on 30th April 5) Participated at the Workshop on Social forestry held at Kalutara from 2nd to 4th May 7) Participated the RSC seminar held at Bandarawela on 13th June and presented a paper on "Soil improvement in tea lands" 9) Participated in the discussion with executives of Kotagala Plantations at . Talawakelle on 6th August 10) Participated in the training programme on "Participatory Forestry" held at Kandy on 12th August at Ratnapura on 28th August at Galle on 9th September and presented a paper on "Soil Conservation measures adopted in tea plantations" ANNUAL REPORT FOR 1997 49 11) Attended the Workshop on Forestry Master Plan held at Colombo on 4th September 12) Attended the annual sessions of the Soil Science Society of Sri Lanka held at Colombo on 10th October 13) Attended and presented the country paper at the study meeting on "Sustainable Fanning systems in Upland areas" held in Japan from 27th October to 2nd November 14) Attended the PGIA Annual Congress held on 20th and 21 st of November 15) Addressed the Field Officers on "Drought management" at the RSC Field day held at Hantane on 17th December b) M.A.Wijeratne 1). Dr.M.A.Wijeratne supervised research projects/dissertaions of two NJPM trainees (Mr.A.M.A.S.Dhanasekara, Superintendent, Keeragala Estate, Kuruwita and Mr. M.S.D.Pinto, Superintendent, Houpe Estate, Kahawatte) and Miss Dammika Jayawardena, undergraduate from the Faculty of Agriculture, University of Peradeniya. 2). Reviewed papers for the TRI journals. 3). Dr.M.A. Wijeratne and Dr.A. Anandacoomaraswamy presented a paper on Impact assessments on tea production of Sri Lanka as affected by climate change at the National Synposium on Climate Change, held in Colombo on March 7, 1997. 4). Addressed the Superintendents/Asst. Superintendents, Field officers and Smallholders on pruning and plucking at 9 Regional Scientific Meetings (RSC) and field days. He also participated in the RSC seminars at various regions as a resource personal. 5). Dr. M.A. Wijeratne was appointed as a visiting lecturer at the University of Ruhuna and he conducted lectures to the undergratuates of the Faculty of Agriculture on harvesting practices of tea. 6). Seminars/field days were conducted for trainee students from Agriculture Schools and routine training of NAB students was also undertaken. 7). Delivered a speech on the "Impact of climate change on tea Plantations in Sri Lanka" at the Planters' Association meeting at Ratnapura. c). K.G.Premathilaka 1). Dr. K. G. Prematilaka presented seminars in the following fields: i). Tea pruning - for small holders at Ratnapura region in Apr.'97. ii) Tea pruning- for the students of School of Agriculture, Karapinche, Ratnapura in October 1997 50 ANNUAL REPORT FOR 1997 in) Weed Management - for Asst. Superintendents and Field Officers at Kottawa Sub Station in November 1997. iv) Tea Pruning - For the students of School of Agriculture, Angunakolapelessa in December 1997. 2). He attended the AGM of the Soil Science Society of Sri Lanka. 3). He attended the following meetings/courses: i) Regional Forest Conservation meeting held at DFO, Ratnapura in August 1997. ii) "Master Trainers Course" on "Safe and Effective use of Pesticides' held at Pannala, Wayamba University, organized by CIC and conducted by Zeneca Agrochemicals, UK from 13th-17th, October 1997. d) R.M.S.S.Rajapakse 1) Presented a paper on "Review of Earthworms" at a staff seminar held at Talawakelle on 9th April 1997 2) Participated in a workshop on "Remote sensing for coastal and forestry resource management" held from 4th-llth June at NARESA Colombo 3) Participated in a Forestry Symposium organized by the University of Sri Jayawardenapura from 12th- 13th December and presented a paper as a co-author on "Land Use planning for agroforestry in tea estates of Sri Lanka" e) M.S.D.L.De Silva 1) M.S.DX.de Siva and P.B.Ekanayake participated in a group discussion held at Talawakelle on "Weed Management in tea plantations" on 11th June and 18th July organized by the RSC 1 Publications 1. Premathilaka, K.G. (1997). Studies on weed management during early establishment of tea in Low-country of Sri Lanka. Ph.D Thesis, University of Reading, London. 2. Amarasekera, A.R., Abesekera, U.P. and Anandacoomaraswamy, A. (1997). The Effect of post-prune operations on soil physical properties and yield of tea. Sri Lanka Journal of Tea Science, 65: 76-82. 3. Janakie Balasuriya and Gail Smith, B (1997). Effect of saturation vapour pressure deficit of air on shoot growth of clonal tea (Camellia sinensis L.) under controlled environmental conditions. Sri Lanka Journal of Tea Science, 65: 46-57. 4. Modder, W.W.D., Kathiravetpillai, A and Sivaram, B. (1997). Organic Farming in Sri Lanka. In: proceedings of Organic Farming Conference held in Calcutta. BIOCHEMISTRY DIVISION Acting Head - I.S.BAbeysinghe 1. General Mr M. W. Silva, Skilled Mechanic, was promoted to Grade V of the Institute's service w.e.f. 14th March 1996. Mr H. Jayaweera, Technical Assistant, was transferred to Tissue Culture unit at Hantana sub station w.e.f. 2nd May. Mr P.B. Chandradasa, Experimental Officer, was transferred back to Biochemistry division w.e.f. 22nd September. Mr A. M. T. Amarakoon, Research Assistant, returned to the Division on the 23rd December after successfully completing his PhD programme in "Therapeutic properties of Black tea" at the Department of Human Nutrition, University of Southampton,UK. Dr I.S.B.Abeysinghe continued to serve as a member of the committee appointed by the Ministry of Plantation Industries on Quality, Standards and Grades of tea produced and exported by Sri Lanka. He served as a member of the Board of Study in Chemical Sciences at the Post Graduate Institute of Science, University of Peradeniya and the Technical Committee on Tea appointed by the Sri Lanka Standards Institution. Dr. Abeysinghe was a member of the Sri Lanka delegation for the 17th Technical committee meeting on tea of International Organization for Standards (ISO/TC34/SC8) held in Colombo in February. Miss Anoma Abeykoon, undergraduate student from Faculty of Agriculture, University of Peradeniya completed her undergraduate project titled "Development and Application of Isozymes Technique for the Identification of Tea Clones" in February. Mr. B.D.S.Kumara, undergraduate student from University of Colombo completed a project titled "Antimicrobial Activities of Ceylon Black Tea" in March. Miss S.C.M.Fernando undergraduate student from Uva Campus completed her undergraduate research project titled "Improvement of quality in the instant tea manufacture" in August. 52 ANNUAL REPORT FOR 1997 2. Project B/PDEV Project Leaders - I.S.B.Abeysinghe and A.C.Liyanage 2.1. Attempts to improve the tea character of the TRI formulated hot water soluble instant tea In an attempt to increase the tea character of instant tea, the inclusion of withered leaf and fermented dhool to black teas in the manufacturing process were studied. Effect of different withering periods and various combinations of withered leaf and fermented dhool were also studied. Table 1 gives details of combinations used in this experiment. Period of Withered Fermented Black Tea Withering (Hrs.) Leaf (WL)% Dhool (FD)% (BT)% 6 10 - 90 12 10 - 90 12 - 16.66 83.34 15 10 16.66 73.34 15 10 - 90 15 - 10 90 15 10 10 80 Table 1 Combination Withering TF% TR% TC B% Tasters Period Comments 10%WL+90%BT 6 1.80 14.95 1.49 25.99 Brownish powder, Coloury, Useful Liquor 10%WL+90%BT 12 1.84 14.09 1.52 28.60 Brownish Powder, Soft Liquor 10%WL+90%BT 15 1.82 13.84 1.43 27.48 Brownish Powder, Light in Cup 10%FD+90%BT 15 1.76 11.82 1.11 24.40 Brownish Powder, Light in Cup 10%WL+16.66%FD 12 1.91 14.72 1.63 34.39 Dark Powder, + 73.34%BT Light, Raw and Unusual Taste 16.66%FD+83.34%BT 15 1.96 13.88 1.65 34.58 Brownish powder, Bright, Fair colour, Lack Strength 10%WL+10%FD 15 1.82 13.88 1.31 27.22 Light Brown +80%BT Powder, Very Light in cup TF=Theaflavins, TR=Thearubigins,TC=Total Colour, B=Brightness ANNUAL REPORT FOR 1997 53 Table 2 Instant tea was manufactured using these combinations and analysed for various chemical quality parameters. Professional tea tasters evaluation was compared with chemical quality parameters. Results of the experiment are given in Table 2. Results indicate that the withering period has an effect on the quality of instant tea. The inclusion of leaves withered for 6hrs. gave a better product compared to inclusion of leaf withered for 12 hours. Inclusion of withered leaf with longer withering periods resulted in tea liquor becoming softer in character. The inclusion of fermented dhools resulted in brighter teas and fermented dhool with 15 hrs withering was preferred when compared to a 12hrs. withering period. The inclusion of 16.66% fermented dhool gave a better product than the inclusion of 10% fermented dhool. A combination of 10% withered leaf and 10% fermented dhool did not produce better instant tea when compared with only 10% withered leaf or 10% fermented dhool. I.S.B.Abeysinghe, A C Liyanage, H. Jayaweera and M.W.Silva 3. Project D/BIOC - Divisional Activities Project Leaders - I.S.B.Abeysinghe and A.C.Liyanage 3.1 Chemical/ biochemical method in the control of the Shot-hole Borer (Xyleborous fornicatus) in the tea plant Results from experiments carried out in the indicated that caffeine may be involved in the resistant mechanism of some tea clones attack by the SHB, although there may be multiple mechanism of resistance and that synthesis, accumulation and translocation of caffeine to infested tissues may be a plant defense response. Since the caffeine content of both susceptible and resistant clones were much higher than the concentration which inhibit growth of the ambrosia fungus, a search was made for the factor which could reduce caffeine activity. It was observed that polyphenols lowered the antifungal activity of caffeine. A collaborative project with the University of Peradeniya and funded by SAREC was started to study the relationship of caffeine content and individual catechin concentrations in the infested and non infested pencil thick stems from clones TRI 2025 and TRI 2023. For this study samples of SHB susceptible clone TRI 2025 and resistant clone TRI 2023 were collected from Attampitiya Estate , Bandarawela. Node and internode portions of these stems were separated. Bark and stem of these sections were analysed separately for Caffeine, Theobromine, Gallic acid, Epicatechin and Epigallocatechin. Results of the analysis are given in Table 3. 54 ANNUAL REPORT FOR 1997 Clone - TRI 2023 Clone - TRI2025 Caffeine mg/g Caffeine mg/g Status Node Inter Node Inter Node Inter Node Inter Bark Node Stem Node Bank Node Stem Node Bank Stem Bank Stem Healthy 5.17 4.86 0.35 0.32 5.9 6.19 0.44 0.34 Infested 9.09 8.15 0.70 0.49 14.64 14.20 1.14 0.97 LSD NS NS NS NS 4.01 4.39 0.37 0.50 Theobromine mg/g Theobromine mg/g Healthy 1.02 1.16 0.22 0.09 1.80 1.98 0.16 0.16 Infested 1.23 1.47 0.11 0.10 1.90 2.04 0.17 0.15 LSD NS NS NS NS NS NS NS NS Epicatechin mg/g Epicatechin mg/g Healthy 1.54 1.49 0.63 0.54 2.3 2.53 0.41 0.38 Infested 1.59 1.47 0.34 0.35 3.25 3.06 0.19 0.10 LSD NS NS 0.21 NS NS NS NS 0.11 Epicatechin gallate mg/g Epicatechin gallate m g/g Healthy 1.3 0.88 0.26 0.24 1.98 1.97 0.18 0.18 Infested 1.52 1.19 0.16 0.18 3.38 3.13 0.10 0.11 LSD NS NS 0.07 NS NS .NS NS 0.05 Gallic Acid mg/g Gallic Acid mg/g Healthy 0.26 0.29 0.02 0.02 0.03 0.36 0.01 0.01 Infested 0.23 0.27 0.01 0.01 0.30 0.36 0.01 0.02 LSD NS NS NS NS NS NS NS NS Table 3 In clone 2025, significant differences were observed for caffeine content in the bark and stem portions of the infested and non infested samples where the infested portions contained higher amount of caffeine. In both clones, significant differences in epicatechin content in the bark were not observed, in infested and non infested portions. However significant difference was observed in stem portions of the internodes. Similar observations were made for epigaUocatechin. Experiments are being carried out in order to study the role of caffeine, epicatechin and epigallocatechin in the SHB resistance mechanism Three unknown compounds were detected and experiments are in progress to identify these compounds. I S B Abeysinghe, S Ratnayake, S Kumar and V Kumar (University of Peradeniya) ANNUAL REPORT FOR 1997 55 3.2 Evaluation of 3000 and 4000 series clones for quality Several clones from TRI 3000 and 4000 series were evaluated for their quality potential during quality season. Initially clones TRI 4063,4053,4052, 3018, 3016, 3072 and 3073 from Venture estate, Norwood, clones TRI 4052, 4071,4078 and 4079 from Sheen group, Pundaluoya, clones TRI 3015, 3020, 3019, 3016, 3018 and 3048 from Stockholm Estate, Upcot were selected by Plant Breeding division for this purpose. Flush samples were collected during quality season and manufactured at the Technology division using miniature manufacturing facility. Made tea samples were analysed for quality parameters and special emphasis was given to flavour characteristics. Made tea samples were sent for tasters evaluation. Results of the experiment are given in Table 4. Clone TF% TR% TC B% TR/TF QI TE 3015 0.69 11.91 1.82 30.52 17.63 0.28 10.5 3016 - - - - - 0.29 12.0 3018 - - - - - 0.53 11.5 3019 - - - - 0.15 9.5 3020 - - - - - 0.36 9.5 3048 - - - - - 0.54 7.0 3072 0.90 11.06 2.37 32.26 12.32 0.43 14.0 3073 0.89 11.95 2.53 30.22 16.09 0.37 11.6 4052 1.25 13.91 2.76 41.75 11.32 0.30 15.0 4054 - - - - - 0.30 - 4063 0.91 11.61 2.21 32.84 13.34 0.28 14.25 4067 - - - - - - 28.0 4071 0.77 12.94 1.87 37.15 16.76 0.39 14.0 4075 0.98 13.40 2.11 35.91 13.72 0.24 12.0 4078 1.28 13.15 2.63 44.40 10.31 0.38 15.6 4079 0.93 13.69 2.62 31.74 14.74 0.45 21.8 4083 0.60 12.00 1.74 29.14 19.90 - 13.0 4085 0.33 13.67 1.61 12.79 41.34 - 12.0 TF=Theaflavins, TR=Thearubigins, TC=Total Colour, B=Brightness, QI=Quality Index, TE=Tasters Evaluation Table 4 Results indicated that clones TRI 4067 and 4079 have the potential to produce flavoury teas. IS B Abeysinghe and J Jayasundara 33 Development and Application of Isozymes Technique For the Identification of Tea Clones At present taxonomic characterization of clones of Camellia sinensis has been restricted to morphological and reproductive triats But in a clone grown 56 ANNUAL REPORT FOR 1997 under different environmental conditions the mode of expression of some of the characters could be altered depending on the environmental conditions. Therefore, one cannot conclude with confidence that the classified clones reflect true differences at the molecular level. Isozymes which are proteins are the direct products of structural genes and they exhibit the potential of reflecting DNA sequence changes and polymorphic individuals can be detected as genetically different. Isozyme analysis of tea clones was carried out as a preliminary attempt to identify tea clones. Active buds were found to be the most suitable tissue for the enzyme extraction. The best combination of tissue to extraction buffer and PVP was found to be in the proportion of 1 .Og of tissue to 2.5ml of extraction buffer and 0.3g of PVP. Thirteen enzyme systems were tested, namely esterase, acid phosphatase (Acph), aspartate amino transferase (AAT), leucine amino peptidase (LAP), alcohol dehydrogenase (ADH), malate dehydrogenase (MDH), glutamate dehydrogenase (GDH), 6-phosphogluco dehydrogenase (6- PGDH), glucose phosphate isomerase (GPI), alkaline phosphatase , phosphoglucomutase (PGM), peroxidase and polyphenol oxidase (PPO). Except for PPO, rest of the systems were all resolved. Out of the twelve enzyme systems which gave positive results, only GPI was found to be polymorphic. The rest of the enzymes appeared to be monomorphic. Apart from tris citrate/lithium borate buffer pH 8.0, electrophoresis was carried out using different buffer systems; tris histidine pH 7 and histidine citrate pH 5.7 on esterase, AAT, PGM , GDH; and peroxidase, AcpH, LAP; respectively to check if polymorphism could be detected with different buffer systems having a pH similar to the relevant enzymes. It was observed that esterase, AAT and peroxidase produced a monomorphic banding pattern while bands did not appear with PGM, Acph and LAP. Embryonic tissues of the clones TRI 2043, 2025, 2024, 777 and ASM4/ 10 at three different stages of germination were analysed using several enzyme systems which included AAT, esterase LAP, Acph, ADH and GDH to check for polymorphism. During the first stage of germination, ie. emerging stage, only AAT produced bands which were monomorphic and the rest of the systems did not give any positive result. During the second stage of germination ie. prior to greening, esterase and AAT produced monomorphic banding pattern. During the third stage ie. greening stage, only AAT produced bands and they appeared to be polymorphic. On repeating with a different batch of seedlings it was found that the banding pattern obtained for AAT although polymorphic was not consistent for some of the clones. Therefore, for further discussion on genetic nature, polymorphic enzyme system GPI was used. Around eighty five clones which included clones recommended by TRI from 2000, 3000 and 4000 series, estate selections, Chinese and Japanese varieties were used for analysis. TRI 3069 was used as a standard to categorize the clones. Representative banding patterns for GPI system are diagrarnmatically illustrated in Figure 1. In all, seven different banding patterns were observed and the clones were grouped accordingly. ANNUAL REPORT FOR 1997 57 Majority of clones showed a similar banding pattern like that of group 2. These were a mixture of clones which included TRI series and most of the estate clones. Out of eleven popular estate clones that were tested for variation in the banding pattern, 9 clones showed a similar pattern while 2 clones appeared to be different. Clone SI06 showed a unique banding pattern indicating that these patterns could.be used to identify the respective clones. These results indicate that although the estate selections are based on outstanding seedling bushes selected from all tea growing regions in Sri Lanka (low, mid and high) they are genetically similar for this particular locus. Parents of most of the clones were traced in order to confirm the parental genotype which was based on the comparison of banding pattern of both progeny and parental genotype. Based on the results it could be confirmed that most of the crosses tally with the results obtained. For some clones the banding pattern does not approve the parentage as non parental bands appear in the individuals. For certain clones parentage cannot be confirmed since they are open pollinated (Figure 1). Four clones common to high, mid, low elevations and Uva were analysed for their isozymic banding pattern to check the GxE interaction (genotype x environmental). From the results it was evident that there was no difference in the banding pattern with elevation indicating that isozymes are environmentally stable. TRI 2025 has been treated with colchicine to produce TRI 3069 and it was expected to be a tetraploid. However, isozyme analysis showed a similar banding pattern for both clones. Analysis of clones for preferred characters ie. drought tolerance, yield, resistance to shot hole borer, eel worm and blister blight in order to detect possible linkages of isozyme loci did not reveal a strict relationship TRI selected clones originated from ASM 4/10 and it is possible that the estate clones also could have been derived from the same clone. The clonal selection criteria is mainly based on high yield, quality, drought tolerance, resistance to pest and disease etc. Therefore, the diversity of commercially cultivated tea clones are minute. This could be the reason for monomorphic zymogrammes obtained for tested protocols. These results indicate that the genetic base of commercially cultivated tea clones have narrowed down. The project was proposed by the Tea Research Board and was carried out in collaboration with the Coconut Research Institute, Lunuwila. This was funded by the Council for Agricultural Research Policy. A C Liyanage, U Fernando (CRT), K P S K Pathirana and A Abeykoon 3.4 Effect of fertiliser on made tea quality 3.4.1. Effect of Sulphate of Ammonia and Urea on made tea quality The quality of tea is determined in the field by a combination of factors namely, genotype, environmental and agronomic. A plant receiving a correctly 58 ANNUAL REPORT FOR 1997 balanced fertiliser mixture should give a reasonable yield and a product acceptable to the customer, provided proper cultural practices and correct manufacturing procedures are followed. There can be many forms of fertiliser to supply a particular nutrient. For example, in Sri Lanka there are two major forms of nitrogen available, that is Sulphate of Ammonia (SA) and Urea. Eventhough SA is expensive than urea the use of SA has been consistent over the past years. A study was initiated to find out the effect of SA and Urea on made tea quality. This trial was carried out at St. Coombs estate using clone TRI 2025. Effect of two different levels of nitrogen 240Kg/ha/year, 360Kg/ha/year and various combinations of urea and SA, 100%SA, 75%SA & 25%Urea, 50%SA & 50%Urea, 25%SA & 75%Urea and 100%Urea were studied. Flush samples were collected from these plots every other week and the yield was recorded. The flush was anlysed for leaf nitrogen levels. The flush was manufactured at the Technology division using a miniature manufacturing facility. Made tea samples were graded and BOP fraction was analysed for quality parameters for made tea. This experiment was repeated 8 times and the results are given in Tables 7 and 8. Combinations TF% TR% TC B% TR/TF 100% SA 0.87 11.97 3.01 27.30 13.88 75% SA+25%U 0.90 12.11 3.02 27.27 13.53 50% SA+50%U 0.88 12.03 2.94 27.30 14.04 25% SA+75%U 0.90 11.86 3.06 26.49 13.33 100%U 0.89 12.15 3.12 27.04 13.76 LSD NS NS NS NS NS TF=Theaflavins, TR=Thearubigins , TC=Total Colour, B=Brightness, Table 7 - Quality Parameters - SA Vs Urea - N Level - 240Kg/ha/y Combinations TF% TR% TC B% TR/TF 100% SA 0.80 a 11.47 2.83 a 26.45 14.40 a 75% SA+25%U 0.91b 12.03 3.07 b 26.45 13.21 b 50% SA+50%U 0.86 11.64 3.05 b 27.82 13.61 25% SA+75%U 0.85 12.20 3.06 b 26.93 14.16 100%U 0.889 b 11.97 2.97 b 27.18 13.35 b LSD NS NS TF=Theaflavins, TR=Thearubigins, TC=Total Colour, B=Brightness, Table 8 - Quality Parameters - SA Vs Urea - N Level - 360Kg/ha/y At 240N level, significant differences in quality parameters were not observed with both forms of fertiliser. But at 340N level, quality parameters Theaflavins Total Colour and TR/TF ratio showed a sigriificant increase with ANNUAL REPORT FOR 1997 59 100% Urea when compared with 100%SA. This is a Collaborative project with the Soils and Plants Nutrition division. IS B Abeysinghe, L S K Hettiarachchi J Jayasundara and H Jayaweera 3.5.1: Effect of Phosphorous on made tea quality Experiments to study the effect of foliar application of phosphorous on quality of made tea were continued in 1997. The clones used were CY9 and DTI. Di Ammonium Phosphate (DAP) and Triple Super Phosphate (TSP) were used for this study. There were four treatments (2% and 4% DAP, 2% and 4% TSP) and a water spray was used as the control. Flush was collected from these plots in the 1st week and 2nd week after foliar application and flush was anlysed for leaf P levels. The flush was manufactured at the Technology division using a miniature manufacturing facility. Made tea samples were graded and BOP fraction was analysed for quality parameters for made tea. The quality of the made tea was assessed by analysing its liquor characters, desirable flavors and organoleptic properties after the 1st, 2nd and 3rd week of application. Tables 9 and 10 give the quality parameters for clones DTI and CY9 clones after 1 and 2 weeks of foliar applications. TF TR TC B TR/TF QI Control 0.67 11.00 2.47 32.29 20.25 0.52 ba 2% DAP 1.00 13.20 2.64 32.42 13.30 0.41c 4% DAP 0.95 12.93 2.79 28.53 13.61 0.43 c 2%TSP 0.98 12.46 2.64 31.55 12.61 0.55 a 4%TSP 0.83 12.02 2.29 30.76 14.50 0.47 be LSD NS NS NS NS NS 0.06 TF=Theaflavins, TR=Thearubigins, TC=Total Colour, B=Brightness, QI=Quality Index, Table 9 - Clone CY9 - Quality Parameters after the 2nd week of foliar application TF TR TC B TR/TF QI Control 1.00 11.21 2.77 31.97 16.09 0.40 2%DAP 0.99 12.11 2.61 33.76 12.08 0.30 4%DAP 1.32 15.47 3.70 32.04 11.72 0.41 2%TSP 1.06 12.41 3.48 31.72 11.61 0.35 4% TSP 0.94 10.43 3.16 33.13 11.09 0.41 LSD NS NS NS NS NS NS TF=Theaflavins, TR=Thearubigins, TC=Total Colour, B=Brightness, QI=Quality Index, Table 10 - Clone DTI - Quality Parameters after the 2nd week of foliar application 60 ANNUAL REPORT FOR 1997 Results indicated that the foliar application of DAP or TSP did not improve the quality parameters when compared with the control after one week of foliar application. However at the end of the 2nd week, application of 2% and 4% of DAP has reduced the quality index significantly in clone CY9. This is a collaborative project with the Soils and Plants Nutrition division. IS B Abeysinghe, L S K Hettiarachchi, J Jayasundara and H Jayaweera 4. Meetings/Seminars Dr. I S B. Abeysinghe attended the 17th International Standards Organization Meeting which was held from 17-19 February in Colombo. Mrs. Jayanthi Jayasundara attended a workshop on NMR at the University of Colombo in February. Dr. I S B Abeysinghe addressed a seminar organized by John Keels Ltd on Flavour Profiles in January and RSC 11 seminar in February on the same subject at TRI. Dr. I S B Abeysinghe and Dr.(Mrs) A C Liyanage attended 1997 China International Symposium on Tea Technology and Tea Culture in Beijing China from May 28-30th and presented papers on "Aroma profiles of teas from various tea growing regions in Sri Lanka" and "A Study on the changes of PPO and peroxidase activity during drying and storage of tea" respectively. Mrs. Jayanthi Jayasundara attended the annual session of the Institute of Chemistry in June. Dr. I S B Abeysinghe presented a paper on "Some recent findings on black tea antioxidants in preventing inflammatory or autoimune diseases" at the International Seminar on Tea and Health held in Colombo from 13-16th August. Dr IS B Abeysinghe attended a workshop on development of a resource pool of assessors for accreditation activities organised by Sri Lanka Standards Institute and conducted by Swedish Accreditation Board (SWEDAC) in October Miss A Abeykoon, final year B Sc student from University of Peradeniya presented a paper on "Development and Application of Isozyme Technique for the Identification of Tea Clones" at the 53rd annual sessions of SLAAS at the University of Ruhuna in December. This paper was based on results obtained from a project carried out in the division 5. Overseas Visits Dr. I S B Abeysinghe and Dr.(Mrs.) A C Liyanage attended the 1997 China International Symposium on Tea Technology and Tea Culture in Beijing, China from 28-30th May. ENTOMOLOGY DIVISION Actg. Head - Sushila I. Vitarana General A proposal by the Director to consolidate the services at the TRI Low Country Station was supported by the Board and as a consequence, it was decided to shift the Division of Entomology to the Low Country Station at Ratnapura. Mrs.S.1.Vitarana was transferred to Ratnapura with effect from 20 th January in order to take up additional responsibilities in the capacity of Officerin-Charge of the Low Country Station. Mrs.S.B.Vithana, Technical Assistant was transferred to Low Country Station with effect from 1 st March. Transfer of other staff was suspended for lack of housing and other facilities at the station. Mr.K. Thirugnasuntharan served as the Convenor/Secretary to the Task Force on Management of the Shot-hole Borer. The Task Force held two meetings to review progress of work; experiments under this programme are reported by the individual principal investigators in relevant sections in this report. Mrs. LAladeniya, Experimental Officer, continued at her temporary posting as Promotion Assistant, Ceylon Tea Bureau, London, on secondment basis for the fourth year in succession. Mrs. Vitarana continued to function as the Convenor/ Secretary of the Consultative Committee on Estates and Advisory & Extension Services' a sub-committee of the Tea Research Board. Mrs. Vitarana continued to serve as a member, representing the Director on the Pesticides Technical and Advisory Committee appointed by the Minister of Agriculture and also, on the National Plant Quarantine Committee of the Council of Agricultural Policy. Mr K. M.Mohotti Research Assistant, returned to UK to continue with the foreign component of his split-program of postgraduate training leading to a PhD. Ms. S. M. Nagahaulla Research Assistant and Ms. S. M. Samarasinghe, Experimenental Officer continued on their practical projects of the programmes leading to PhD and M. Phil respectively . Mr. L S. Abeysinghe, Experimental Officer was transferred to TRI Mid Country Station, with effect from 1st September. 62 ANNUAL REPORT FOR 1997 Mrs. Viratarana participated at meetings of the Cordinating Committee on the Implementation of Montreal Protocol, of the Minitstry of Forestry and Environment. A project proposal submitted to the Ministry of Transport, Environment and Women's Affairs in 1995 was accepted for funding through the NORAD and a sum of Rs.4,622.000.00 was made available with effect from January 1997, for a study on substitutes for Methyl Bromide as a nematicidal agent in tea plantations. The research activities of this project are being reported under "Project: MeBr". Research Activities 1.0. Project B/NEMA - Development of Integrated Management Strategies for the control of Plant Parasitic Nematodes causing economic damage to tea. Project Leader - Sushila I Vitarana 1.1 Screening of Promising clones for natural resistance/ tolerance / susceptibility to parasitic nematodes attacking tea. The objective of this programme is to screen new release clones against the different species of nematodes that cause damage, to tea. 'This is a continuous programme with different batches of clones being tested each year. 1.11 N1A (1996) Screening TRI 4000 series clones against the root-lesion nematode^ Va/y/cnc/Vus loos] with reference to build up of nematode population in root and soil and growth of tea plant (16 treatments x 4 replicates in Randomized Complete Block design Nematology Experimental Area, TRI, Talawakele) TMrteen test clones and three standards namely TRI 2024 (susceptible), TRI 2025 (tolerant) and DT 1 (resistant) planted in infested soil. The test clones included TRI4002,4003 ,4004 ,4005 ,4014 ,4015 ,4019 ,4024 ,4042 .4047 , 4053,4085,and 4089. IS. I. Vitharana D. D. Liyanage, G. P. Udumulla, and N . Navaratne 1.1.2. N I B . Screening TRI 4000 series clones against the root-lesion Nematode, Radopholus simlis with reference to build up of nematode population in root and soil and growth of the tea plant (1994) (16 treatments x 6 replicates in RCBD, TRI Mid Country Station, Hantana) A ANNUAL REPORT FOR 1997 63 The experimental beds planted with anthurium in 1996 were reinnoculated with pure lab cultures of R. similis in build up the population before planting the test clones. However, the population levels in the test beds did not reach required levels and it was arranged to shift the experiment to a naturally infested old nursery site on Galaha Estate where the site was prepared in December and planting of cuttings was scheduled for 1998. Test clones included TRI 4002, 4014, 4020, 4047,4048, 4049, 4051, 4052, 4054, 4059 and TRI 2025 as the standard. S.I.Vitarana, U.B.Herath, B.Suresh Kumar and G.P.Udumulla 1.2. Studies on Biological Control Agents Field studies of a PhD project based on biological control of tea nematodes using Pasteuria penetrans,, commenced in February. Six experiments have been laid down up to November and the work is in progress. K. M.Mohottie. D. D. Liyanage, N. Navaratne, P. K. Jayawickrema and G. P. Udumulla 1 3. Cultural Control 1 3.1. Studies on Botanicals The plant species which were earlier proved to be immune to Pratylenchus loosi, Radopholus similis and Rotylenchulus reniformis were being screened to check for the presence of any nematicidal properties. It was arranged with Dr.Ingo Kohler of Bayer AG Germany to isolate and concentrate the active ingredient of Marigold Tagetes sp. S.I.Vitarana. I.Kohler and D.D.Liyanage. 2.0 Project: MeBr To find substitutes for methyl bromide for eradication of plant parasitic nematodes with environmentally acceptable fumigants and Biological Control Agents and other means Project Leader - Sushila I.Vitarana Studies under this Project commenced in mid 1896 pending approval by the external funding agency. 2.1 Biological Control agents N 315 -Isolation of endemic biological control agents of tea nematodes and formulating methods of augmenting their activity. 64 ANNUAL REPORT FOR 1997 The study was undertaken with a view to isolating endemic biological control agents of tea nematodes such as fungi, bacteria and soil micro-organisms. Cultures of P. Loosi at Talawakeile and H. Simil lis at Hantane laboratories were maintained for bioassay purposes. Laboratory cultures of prospective nemato-pathogenic agents collected from field soil in December. The study is in progress. S. I. Vitarana, D. D. Liyanage, N. Navaratne and U.B. Herath 2.2. Direct Planting of Infested Lands 2.2.1. Direct Planting using Alternatives to Rehabilitation of land under grass to eradicate soil borne parasitic nematodes N. 316 - (MeBr No. 1)) -1996 N. C. Blook,, Agarakanda Estate, Agarapatne The trial initiated in 1996 in a nematode infested and abandoned land with the following treatments in a split- plot factorial design was in progress : 2C x 2N x 4IC where. S = the main treatments of Soil amendments at planting Si = Bottom .3/4 of hole filled with equal volumes of soil + decomposed spent-tea So = No soil amendment. N = Nematicidal treatment in sub plots, No = Nonematicide Ni = Nemacur (7 g)) in Planting hole I = Insecticidal treatment to control white grubs that are attracted to the organic matter in the planting hole, as well as for its nematicidal activity. Ij = Suscon Fore (2 g) 12 = Suscon Fore (3 g) 13 = Suscon Fore (4 g) 14 = Suscon Fore (5 g) One assessment on the population of nematodes in the soil and roots carried out in September has shown the following results : a. nematode population is still being retained in the plants, though to different degrees in different plots b. there were more nematodes in the roots in the case of Plots receiving no soil amendment nor nematicide; c. However, there was no statistically significant difference between treatment levels or in the interacions between treatments at this stage. The experiment was in progress. S. I. Vitarana. D. D. Liyanage, N. Navaratne, Y. Konaratne (casual worker), G. P. Udumulla, and Gaminie Jayawardena, Director for Agarakanda Estate. ANNUAL REPORT FOR 1997 65 2.2.2 Direct planting with new Nematicides Four experiments in different agroclimatic areas, were initiated in the current year as given below, to study the feasibility of treating the planting holes with chemical and field as an alternative to 2-year rehabilitation under grasses. In the case of organic nematicidal agents, the material that is easily available in a given area was used in the experiment: N. 319 - (MeBr 13) - Field No. 6, New Forest Division, Cocagala Estate, Madulsima - commenced August '97 (4 Treatments x 3 Replicates) The experimental blocks were planted with the clone TRI 2025, in November, in replicated Randomized Block Design with the nematicidal agent incorporated in to planting holes with an untreated control plot (T4) for comparison : Tl - Tobacco dust 50 g per plant (mixed into the soil in the planting hole 2 days prior to planting) T2 - firprinol ("Rugby") @ 7g per plant T3 - fenamiphos ("Nemacur" @ 7g per plant (standard) A basal treatment with T200 fertilizer mixture @ 20g per planting hole was given to all plants at the time of planting. The experiment was in progress. S. I. Vitarana, D. D. Liyange, N. Navaratne and Subash Jegatheesan, Superintendent, Cocogala Estate N 324 (MeBr 2) - 1997 N C , Nayapane Estate (June 1997), (4Treatments x 3 Replicates) The experimental blocks were planted in Randomized Block Design with the chemical nematicides incorporated in to planting holes; Treatments Tl - fiprinol ("Rugby") @ 7g per plant T2 - fenamiphos ("Nemacur") @ 7g per plant (standard - chemical) T3 - carbosulfan CR ("Suscon Fore") @ 5g per plant T4 - "Veriver" grass planted in the inter-row (standard - organic) Visual observations were made regularly: Best initial growth was exhibited by the plants in T4, shade provided by the grass is of added advantage to the young plants; plants in Tl exhibited phytotoxicity during the first 3 weeks after planting but, they recovered thereafter, indicating that a pre-planting period of 3-weks is 66 ANNUAL REPORT FOR 1997 necessary in the case of firprinol; treatments T2 and T3 were similar in terms of general growth, however, the least number of casuaties by the end of the year, in T2. Maintenance operations were carried out. The experiment was in progress. S. I. Vitarana, D. D. Liyanage, N. Navaratne, U. B. Herath, G. P. Udumulla and Y. Konaratne N 325 (MeBr 3) - 1996 NC Block, Courtlodge Estate, Kandapola (4 Treatments x 3 Replicates) The experimental blocks were planted with the clone PK 2, in three replicates, in September and in Randomized Block Design with the chemical nematicides incorporated in to planting holes. All plots received a basal mixture of 250-300 g tea waste and 3 g "Sscon Fore" (as insurance against white grubs that are attracted to organic matter). Treatments were as follows : Tl - fipronil ("Rugby") @ 7g per plant T2 - fenamiphos ("Nemacur") @ 7g per plant (standard) T3 - compost (cowdung + weeds) @ 800g per plant The untreated control (T4) received only the basal mixture Unlike at experimental sites of N 824 and in N 330. fiprinol did not elicit any phytotoxicity symptoms; the difference could be explained as due to the lower ambient temperature and higher organic content in the soil at Courtlodge as against the other two sites. The experiment was in progress. Since PK 2 is a tolerant. Clone, the future assessments would be based on the nematode populations. S. I. Vitarana, D. D. Liyanage, N. Navaratne, G. P. Udumulla, Y. Konaratne and D. Gankanda Gankansa (Superintendent, Courtlodge Estate) N 330 - (MeBr 8) - F. No. 10. Yelverton Division, Queenstown Estate, Hali Ela (4 Treatments x 3 Replicates) The experimental blocks were direct Planted in Randomized Complete Block design in October '97. The treatments applied at planting were being compared with untreated control: Tl - fipronil ("Rugby") @ 7 g Per plant T2 - fenamiphos ("Nemacur") C 7 g per plant T3 - "Neemazal 1%" @ 850 ml per ha, sprayapplication at planting ANNUAL REPORT FOR 1997 67 Phytotoxicity was observed in the form of wilting and arrested growth in the case of Tl . "Neemazal" treated plants exhibited the best growth.. The experiment is in progress. S. I. Vitarana,. D. D. Liyanage, N. Navaratne,, G. P. Udumulla andY. Konaratne 2 2.3. Use of Organic Amendments in Direct Planting N 326 (MeBr 4) - Field No. 9, Old Factory Division, Hopton Estate, Lunagala, (4 Treaments x 3 Replicates) The experiment plots were planted in December '97 with following in Randomized Complete Block design: Tl - fenamiphos ("Nemacur") C 7 g per plant (standard) T2 - neem formulation ("Nemacur 1%") 850 ml/ha T3 - fresh neem (Margosa) leaf thatch T4 - untreated control Only the treatments 'Tl & T2 were applied by the TRI staff at the time of Planting. In the case of T3. the Superintendent (who is e collaborator in this study) was to carry out thatching with neem leaf. S. I. Vitarana, D. D. Liyanage,. N. Navaratne,. G. P. Udumulla, Y. Konaratne and M. Kariapperuma, (Superintendent and S. Godage, (Asst. Superintendent. Hopton Estate) N 328 - (MeBr 6) Field No. 3 (NC 97), Hanford Estate, Deniyaya. (4 Treatments x 3 Replicates) Direct planting of the experimental plots were carried out on Randomized Complete Block design, in October with untreated control (T4) for comparison Tl - All plants Per plant, thatching with Tithonia diversifolis (wild Sun flower) T2 - "Vetiver"" grass planted in the inter-row T3 - "Nemacur " 7 g per plant at planting were given a basal treatment of 3 g of "Suscon Fore" The experiment was in progress. S. I. Vitarana, A. K. Prematunga, D. D. Liyanage,P. K. Jayawickrema, and R. Dharmabandu, (Superintendent of Hanford Estate). 68 ANNUAL REPORT FOR 1997 2 2.4. Eradication of nematodes in infested Young Clearings N 327 (MeBr 5) - Kelliewatta Estate A study on block-infilling of infested tea with organic and inorganic nematicidal treatment in place of rehabilitation commenced in March; treatment and planting of the experimental blocks (in Randomized Complete Block design) were carried out in December. The experiment design is of Replicated Completely Radomized Blocks: Tl - Thatching with "Vetiver" grass T2 - fenamiphos ("Nemacur") @ 7 g per Plant T3 - Thatching with tobacco waste (Tobacco dust-TD)) T4 - Untreated control. The experiment was in progress. S. I. Vitarana. D. D. Liyanage, N. Navaratne, G. P. Udumulla and Y. Konaratne N 332 (MeBr 15) - 1990 NC F. No. 1A. Galaha Estate,, Galaha Commenced in October. In this trial, treatments are being carried out in an infested immature clearing under mixture of clones namely DN, TRI '2025 and '2026. a Randomized Complete Block design, with 3 replicates : Tl - fenamiphos ("("Nemacur") @ 7 g per Plant (standard) T2 - thatching with fresh, green neem (Margosa) leaf, @ 1 kg per plant, repeated at regular intervals, depending on the rate of decomposition. T3 - furrow application of sugar cane molasses pretreated with weeds re moved from the tea land and decomposed for one month @ 500g Per plant. T5 - untreated control One round of treatment application, where applicable was carried out in November. Best recovery plants were exhibiting symptoms of nematode infestation at commencement of trial was seen in the Tl plots, by end of the Year. S. I. Vitarana, U. B. Herath, P. Udumulla and B.SureshKumar N 332 (MeBr 20) - F. No.. 1A„ Galaha Division, Galaha Estate, Commenced in October. One trial similar to MeBr 15 was started to test higher dose of fresh neem leaf in an infested immature clearing under one clone. TRI 2025, in a Randomized Complete Block design, with 3 replicates . Treatments are scheduled for January: ANNUAL REPORT FOR 1997 69 Tl - fenamiphos ("Nemacur") @ 7 g per plant (standard) T2 - thatching with fresh, green neem (Margosa) leaf. 1 kg per plant repeated at regular intervals, depending on the rate of decomposition. T3 - furrow application of sugar cane molasses Pretreated with weeds removed from the tea land and decomposed for one month @ 500g Per plant. T5 - one month control S. I. Vitarana, U. B. Herath, P. Udumulla and B. SureshKumar 2.3. Nematicidal agents for nurserv soil sterilization (alternatives to Methyl Bromide) 2.3.1. Chemical sterilants: N 334 (MeBr 17) - St. Coombs - Basamid ExptSep '97 Nematology Nursery Nursery experiment was carried out to check the Period of toxicity due to "Basamid" fumigation' because, there was the need to shorten the period of nursery work: Fumigated soil was bagged and the cuttings were planted after one. two, three and four weeks from fumigation in Tl , T2. T3, and T4 respectively. Dates of treatment were staggered in such a way that the assessments could be carried out on the same day in all plots. The observations are summerized below : Treatment % Plants affected (Period alter fumigation) (dead + scorched)) One week 11 % Two weeks 8 % Three weeks 5 % Four weeks 1 % It was concluded that, soil treated with "Basamid" can be planted with tea cuttings after four weeks for with the chemical. S. I. "Vitarana and P. Udumulla 2.3.2. Soil Solarization as a means of soil sterilization N 320 (MeBr 11) -Tea Nursery, Ova Highland Estate. Bandarawela,, Solarization under clear-polythene, by itself and in combination with nitrogen fertilizers to impart synergistic effect are being tested in Replicate trial, using 150-plant area per plot: 70 ANNUAL REPORT FOR 1997 Tl - Untreated control (the current estate Practice) T2 - treated with 'Basamid" as per current TRI recommendation T3 - bagged infested soil under 3-week solarization T4 - bagged infested soil under 6-week solarization T5 - bagged infested soil under 9-week solarization T6 - infested soil treated with urea @ 5 kg per cube soil, bagged and solarized for 3 weeks T7 - infested soil treated with (NH4)2S04 @ 5 kg per cube soil, bagged and solarized for 3 weeks T8 - infested soil solarized for 6 weeks in bulk T9 - infested soil solarized for 9 weeks in bulk Treatments commenced in August. Cuttings were planted in September, October and November in the 3-week, 6-week and 9-week solarized plots respectively. The experiment is in progress. S. I. Vitarana, D. D. Liyanage and N. Navaratne N 321 (MeBr 12) - Tea Nursery, Ury Group , Passara. Solarization under clear-polythene, by itself and in combination with nitrogen fertilizers to impart synergistic effect are being tested in a 3-Replicate trial, using 1000-plant area per plot and 6" x 3" size bags : Tl - infested soil solarized for 10 weeks in bulk T2 - bagged infested soil under 10-week solarization T3 - infested soil treated with urea @ 5 kg Per cube soil, bagged and solarized for 10 weeks T4 - infested soil treated with (NH4)2S04 @ 5 kg Per cube soil, bagged and solarized for 10 weeks T5 - bagged infested soil fumigated with Methyl Bromide @ one 500g can per 1000 plants (standard) T6 - untreated bagged soil covered with Mana up to the time of planting cuttings T7 - infested soil in bags, planted with cuttings and immediately covered with plythene for long term solarization (moisture is recycled under polythene)) The experiment was in Progress. / S. I. Vitarana,. D. .D Liyanage and N. Navaratne N 323 (MeBr 10) - Tea Nursery, Moray Estate, Maskeliya Preparatory work at this nursery commenced in November for a solarization experiment similar to N 320. Treatments were sheduled for the period after dry weather sets in, in January '98. S. I. Vitarana,, N. Navaratne and P. Udumulla ANNUAL REPORT FOR 1997 71 N 336 (MeBr 14) - Tea Nursery, Galaha Division, Galaha Estate, Jan-98 ; Nematode infested nursery which had been abandoned due to nematodes was prepared in November to conduct an experiment to test the efficacy of soil solarization by it self and in combination with other agents,, as given below : Tl - soil mixed with urea @ 900 g/450 bags, bagged and kept under 6-week solarization T2 - soil mixed with urea @ 900 g/450 bags, T3 - soil mixed with (NH4)2S04 urea @ 900g bagged and kept under 6-week solarization T4 - soil mixed with (NH4)2S04 urea @ 900 g/450 bags, bagged and kept under 8-week solarizatiom T5 - soil mixed with "Basamid" @ 125 g/460 bags, bagged and kept under 6-week solarization T6 - soil mixed with "Basamid" @ 126 g/450 bags, bagged and kept under 8-week solarization T7 - bagged infested soil-under 6-week solarization only T8 - bagged infested soil under 8-week solarization only T9 - Untreated control (the current estate practice) T10 - treated with MeBr according to current recommendation Preliminary work was carried out and treatments were scheduled for early 1998. S. I. Vitrarana,, U. B. Herath,, P. Udumulla and B. SureshKumar 2.4. Purification of nematode contaminated water for nursery use; LN 1 - (MeBr) 19) - Tea Nursery TRI Centre,, Deniyaya. A study commenced to test the sedimentation efficacy of an arrangement of three "Plastisheli" tanks which are easily available in any area to clean contaminated water that is the only source available to the nursery at present. The study is in progress. S. I. Vitarana,. A. K. Prematunga and P. K. Jayawickrema 2.5. Miscellaneous Studies under MeBr Project: N 329 (MeBr 7) - Survey on the usage of Methyl Bromide and other nematicidal agents in the nursery and in fields on tea estates. '97 A questionnaire was prepared to collect data from the estates where nematode infestationhas been recorded. A seperate vehicle was being awaited 72 ANNUAL REPORT FOR 1997 to start the survey proper by the end of the Year. S. I. Vitarana,, D. D. Liyanage, N. Navaratne, U. B. Herath, G. P. Udumulla, A. K. Prematunga and Y. Konaratne 1.3.0 Cultural Control 1.3.1. Studies on Botanicals The plant species which were earlier proved to be immune to Pratylenchus loosi, Radopholus simi lis and Rotylenchulus reniformis were being screened to check for the presence of any nematicidal properties. S. I. Vitarana, D. D. Liyanage, N. Navaratne, U. B. Herath and G. P. Udumulla 4.0 Project B / PECO : Pest Ecology and Management of pests with special reference to Shot- hole Borer and Up-country live wood termite. Studies carried out under the project B/SHBO in relation to the management of the Shot-hole borer (Project Leader - K. Thirugnanasuntharan are being incorporated under B/PECO as both these Projects include activities that come under those of the 'Task Force on Shot-hole Borer. Project Leader - L. D. Amarasinghe 3.1. Resistance of tea clones to the Borer (in collaboration with Plant/Breeding Division) 3.2. Effect of systemic fungicides on Borer buildup (B/SHBO) The effect of systemic fungicides on the growth of the ambrosia fungus which is the food of the brood of the borer, is being studied in order to assess their indirect effect on the development of the borer. This study is in collaboration with the Plant Pathology Division. E 292 - TRI Uva Station, Passara.(in collaboration with Plant Pathology Division) In this trial, initiated in November 1996, overgrown nursery plants of the susceptible clone TRI 2025 were being treated with two systemic fungicides in two methods of application, at three concentrations, at three weekly intervals. It was planned to artificially introduce the Borer beetles to the plants in 1997. The results did not show any significant difference between treatments. K. Thirugnanasuntharan, A. Balasuriya, A. Abeysekera, and P. D. P. de Silva ANNUAL REPORT FOR 1997 73 3.3. Effect of high potash fertilizer E 289 - Attampettia Estate, Ettampitiya (1996) A study on the effect of high potash on nursery stage of growth was initiated in collaboration with Soils and Plant Nutrition Division in 1996 on factorial design: Treatments : 3C x 4K x 3R where, 3C = 3 clones : TRI 2025 (Susceptible) DN (moderately susceptible) TRI 3041 / 4070 (resistant) 4K = 4 Potash levels : @ 0 g, 2.3 g, 5.6 g, 8.4 g. 3R = 3 replicates Treatments commenced at the time of bagging of soil in the nursury and continued at fortnightly intervals after 2 months from planting of cutting. Post infestation was not observed while the plants were in the nursury. Nursury stage of the trial was terminated in September and the plants were transplanted in October in F. No. 02, First Division of the same estate. They were planted on Randomized Block Design (15 plants of one treatment combination going in to one plot in a given replicate). The following treatments were superimposed on the field planted blocks. Tl - T 200 (@ 25 g) + 0.0 g MOP per plant per application T2 - T 2 0 0 ( @ 2 5 g ) + 1 . 5 g M O P - d o - T3 - T 200 ( @ 25 g) + 3.0 g MOP - do - T4 - T 200 ( @ 25 g) + 4.5 g MOP was carried out in one application of fertilizer treatments December. E290 - Tea Nursaury TRI Mid Country Station, Hantane. This is a replicate of the experiment E289. Nursury stage of the trial was terminated in September and tyhe plants were transplanted in October in Field No. 4, Factory Division of Hantane Estate, Kandy. They were planted on Randomized Block Design using 12 plants of one treatment combination going in to one plot in a given replicate. L. S. K. Hettiarachchi, L. D. Amarasinghe, K. Thirugnanasuntharan, L. S. Abeysinghe, A. R. Abeysekera, B. SureshKumar and U. B. Herath 4.2.1. E266- Screening of Insecticides against SHB F. No. 17, First Division, Attampettiya Estate (1994) Post-treatment assessments of branch sampling for shot-hole borer infestation were carried out; mean number of galleries and mean population 74 ANNUAL REPORT FOR 1997 per 30 cm of primary branch were assessed commencing from 15 & 19 months after prune in October 95 (PI) and March 96 (P2) respectively. The chemical treatments included the following: Tl - Admire SL200 at 500 ml ha' T2 - Acimire SL200 at 500 ml ha 1 T3 - Ekalux 25EC at 1000 ml ha 1 T4 - Ekalux 25EC at 2000 ml ha-' T5 - Chlorpyrifos 25EC at 4500 ml ha"1 T6 - Chlorpyrifos 20EC at 1500 ml ha 1 T7 - Untreated control Table 1: Mean number of galleries per 30cm of Primary Branch (number of samples =30) Insecticide Months after pruning Treatment 19 21 15 18 20 22 25 Oct. Dec. Jan. Apr. Jun. Aug. Nov. Tl 3.3 3.6 2.8 3.0 3.7 4.4 4.4 T2 3.2 3.8 2.5 3.2 3.5 4.5 4.0 T3 4.3 4.4 2.2 3.5 4.1 4.8 4.3 T4 3.2 3.6 1.8 1.9 3.1 3.3 3.3 T5 3.0 4.0 1.6 2.8 3.1 3.6 3.4 T6 3.3 3.3 1.7 2.6 3.5 3.6 4.3 T7 3.5 4.0 1.8 2.4 3.0 4.1 3.6 T8 4.3 3.3 2.6 3.4 4.0 5.2 4.2 Table 2: Mean population per 30cm of a primary Branch (number of samples =30) Insecticide Months after pruning Treatment 19 21 15 18 20 22 25 Oct. Dec. Jan. Apr. Jun. Aug. Nov. Tl 8.0 7.5 2.9 6.1 4.1 2.5 4.4 T2 12.6 7.2 5.3 4.2 5.1 1.6 5.3 T3 5.5 6.3 3.2 3.8 5.9 2.1 5.3 T4 6.8 7.2 2.8 3.0 2.9 1.8 4.0 T5 5.0 8.6 3.6 4.4 3.0 3.2 4.0 T6 4.7 8.6 3.7 3.5 5.2 2.2 3.5 T7 6.5 8.4 5.0 2.1 3.7 2.8 4.2 T8 15.0 10.1 5.1 3.7 4.8 3.9 3.7 PI = Plots pruned in October 1995 P2 = Plots pruned in March 1996 The data indicated that the treatments were not significantly different from the untreated control. L. D. Amarsinghe, L.S. Abeysinghe, A. Abeysekara, and P.D.P. de Silva ANNUAL REPORT FOR 1997 75 4.2.2. E 275 Serening of insecticides against SHB in Young tea (Field No. 3, NP Division, New Peacock Estate, Pussellawa 1995) Chemical treatments are the same as in E266 Branch-breakage was used as a measure of the infestation in each plot, at one assessment carried out in November. The experiment is in progress. L. D. Amarasinghe, L. S. Abeysinghe, U. B. Herath and P.D.P. de Silva Table 3: Total branch breakage (4 plot each consisting average 40 plants). Treatment Block planted in 1995 Block planted in 1994 (2 years) (3 years) Tl 23 6 T2 21 5 T3 24 14 T4 27 13 T5 12 12 T6 18 13 T7 16 9 T8 26 6 Results will be analysed at the first formative pmning. 4.23. E 294 - Use of bio-control agents in reducing shot- hole borer This study was intitiated during the latter part of the year. Three isolates of the entomopathogenic fungus Beauveria bassiana namely. I-92-76A. 1-94-477, were imported from Commonwealth institute of Biological Control, UK. Pure cultures were raised on Molisches Agar plates and blastospores were obtained by growing the isolates in liquid cultures of the same medium. The preliminary bioassays were carried out by application of the test material in the form of spores, with a brush on borer infested branches (each 30cm long) held in polythene bags and replicated 4 times. Mortality/survival of Shot-hole borer was assessed after 8 days. The results showed that only the adult beetles were killed by the pathogen and the immature stages wer unaffected. The study is in progress. L.D. Amarasinghe, L.S. Abeysinghe, A. Abeysekara 76 ANNUAL REPORT FOR 1997 4.3. Host volatile for mass trapping a. Identification of Kairomones : Bark volatiles of the host and non host type of plants and of the infested and uninfested stems on such plants were studied and the progress of the study was presented at the bi-annual meeting of the SAREC project held at Institute of Fudimental Studies, Kandy, in July. The study was repeated with samples from Attampettiya Estate to confirm the first findings.The experiment is in progress. L.D. Amarasinghe, Y. Konaratne and I.S.B. Abeysinghe b. Effect of presently recommended shade trees as diversionary hosts to the Borer (in collaboration with Agronomy Division) E 286 - Field No. 14, 2nd Division, Attampetiya Estate, Attampitiya; Shade combination is Grevillea robusta and Eriythrina lithosperma Pre treatment assessment on shot-hole borer infestation was carried out in June. Mean number of galleries per 30cm of a primary branch was found to be the same (6.7) in all plots indicating uniform and heavy infestation. Plots were pruned in October and assessments were scheduled/commnced in December 1998. E 287 - Field No. 1, Sirimedura Division, Kiruwanagaga Estate, Deniyaya. Shade combination is Albizzia moluccana and Giricidia sepium Plots were pruned in June. Post treatment assessments were scheduled to commence in June 1998. E 288 - Field No. 6, NP Division, New Peacock Estate, Pussellwa. Shade combination is Grevillea robusta and Erythrina lithosperma Pre-treatment assessment was carried out in June. Mean number of galleries per 30cm of a primary branch was found to be 6.2 for Plots with shade and 7.6 for plots with no shade. All plots were pruned in July. Post- treatment assessments were scheduled to commence in September 1998. A. Kathirvetpillai, L.D. Amarasinghe, K. Thirugnasuntharan, L.S. Abeysinghe, A.R. Abeysekera and U.B. Herath C. Effect of times and methods of pruning in reducing shot-hole borer (in collaboration with Agronomy Division) ANNUAL REPORT FOR 1997 77 E 283 - Field No. 7, First Division, Attampitiya Estate, Ettampitiya : Treatment in the form of pruning in January and pruning in April were carried out. (October pruning had caried out in 1996). Based on visual obser­ vations made in June and December, post treatment assessments were sched­ uled to begin in February 1998 (16 months from prune). A. Kathirvetpillai, L.D. Amarasinghe, K. Thirugnasuntharan, L.S. Abeysinghe and A.R. Abeysekera& RD.R de Silva E 284 - Field No. 1, Kiruwanagnaga Estate, Deniyaya (pruned in October in 1996) Treatment in the form of pruning in April was carried out. (October pruning had been carried out in 1996). Visual observations made in August, October and December indicated that even green stems measuring about 0.5cm in diameter also, were infested in plots pruned in October '96. Data collected by the end of the year is given in Table. Table - 4 Total branch breakage Prune time Type of Prune Total Branch Breakage Months after Prune 4 6 10 12 October '96 Cut-across t 0 202 65 Normal - 95 320 80 Rejuvanation - 142 160 44 April '97 Cut-across 25 29 - - Normal 37 19 - - Rejuvanation 4 18 January '98 Cut-across - - - - Normal - - - - Rejuvanation - - - - Future assesments are scheduled for a period up to 36 months. A. Kathirvetpillai, L.D. Amarasinghe, K. Thirugnasuntharan, L.S. Abeysinghe and A.R. Abeysekera E 285 - Field No. 6, N.P. Division, New Peacock Estate, Pussellawa. Pruning scheduled for April and October were carried out. Assesments commenced in September. The experiment is in progress. A. Kathirvetpillai, L.D. Amarasinghe, K. Thirugnasuntharan, L.S. Abeysinghe and A.R. Abeysekera& P. D. P. de Silva 78 ANNUAL REPORT FOR 1997 4.4 Clonal Selection for resistance to the UCLWT & SHB E 271 - Field No. 5, of Lower Division, Mocha Estate, Maskeliya (B/PECO). During the year, 20 more seedling bushes were selected from this field in June. Root samples were bioassayed by the methods adopted in 1996. How­ ever, none of the 20 selections proved to be promising as resistant to the up country Live wood Termite. This experiment will be continued to complete 200 selections in the above location. L.D. Amarasinghe, L.S. Abeysinghe and RD.P. de Silva LE 83 - Clonal Selection for pest resistance inclusive of Shot-hole Borer (B/SHBO) Field No. 5B, Maratenna Division, Balangoda Group, 100 bushes were selected from old seedling bushes in November and 72 of the best were se­ lected out for propagation in December. Clonal cuttings were established in the estate nursery of Maratenna Division. Generation of cuttings per mother bush varied from 10 to 270. However, acording to census taken after establish­ ment, it was observd that they had 100% success. S.I. Vitarana, A.K. Prematunga, E.St.E.R.Perera, Ramaiya Kanganie (retired Kanganie, Maratenna Division), Mr. K.C. Munaweerahettie, (The Superintendent) and K. Thirugnasuntharan 4.7 Early Detection of Up-country Live wood Termite Further development of the equipment, High Gain mstrumentation am­ plifier (HGIA) is in progress. K. Trurugnanasuntharan 5. PROJECT B/TERM : Pest ecology and productivity of tea lands in relation to the containment and management of the live-wood termites in the Low-country. Project Leader - Sushila I Vitarana 5.1 Clonal Screening Clonal resistance to the live wod termites is being studied on replicated plot trails designed in collaboration with the Plant Breeder as well as in mother bush blocks estblished in the low-country. ANNUAL REPORT FOR 1997 79 5.1.1 Field Screening ; LE 22 (ix) -1 Talangaha Estate The clones that were being studied included NTL211, NTL12, NTL262, NTL53, TRI3029, NTL274, TRI3052. There were no experimental operations scheduled for this year. The experiment is in progress. S.I. Vitarana, S.M. Samarasinghe, and RK. Jayawickrema LE 50 - Hapugastenna Estate, LAT Division Experimental blocks were being maintained with casualties being infilled. S. I. Vitarana, E.StE.R. Perera and A. K. Premathunga LE 78 - Hapugastenna Division, Ratnapura F. No. 4, Hathdaraganga Division 50 new selections made in march 1996 were being raised in the nursery. The selections turned out to be very slow growing in the nursery, perhaps be­ cause they were the most hardy and selected for total immunity for termite attack. These are meant to enter furture breeding programmes. S.I. Vitarana, S.M. Samarasinghe, E.StE.R. Perera and A.K. Premathunga 5.2 Laboratory Screening of plants against the low country live wood termite LE 80(a) - Bioassay of test clones with TRI 2023 as the standard, using dry power of stem material was carried out adopting the "4-channel perspex olfac­ tometer" testing 4 clones at a time. Six replicates were run simultaneously. The following clones were being tested: 4048,4059,4002,4003,4054,4088, 4089, 4087, 4004, 4005, 4015, 4019, 4024, 24048, 4059, 4002, 40034054, 4088, 4089, 4087, 4004, 4005, 4015, 4019, 4024, 4031& PGI (Pambegama selection), with TRI 2023 as control. Data was being prepared for analysis. The study is in progress. S.I. Vitarana, A.K. Premathunga and E.StE.R. Perera LE 80 (b) - Bioassaying of plant extracts for their kairomonal and allomonal properties (using olfactometers) Two different models of olfactometers were used to test chemical ex­ tracts. Extractions were done both as cold extraction and by solvent distillation extraction (SDE), using hexane, methonol & dichloromethane. Extraction was replicated at TRI laboratories and at chemistry Department, University of Peradeniya. Both types of extracts elicited similar response from the test in- 80 ANNUAL REPORT FOR 1997 sects (alates) even though the two extracts had dissimilar gas chromotograms. The test material included the clones of the series TRI 2000, 3000 and 4000. The same extracts were used for feeding behaviour studies using two methods namely, filter paper incorporated with extracts in petri dish ensemble, secondly incorporating the extract in artificial diet medium. The observations corroborated the findings of the study of LE 80 (a). This study is in progress. S.M. Samarasinghe, I.S.B. Abeysinghe and Vijaya Kumar (University of Peradeniya) LE 81 - Hapugastenne Estate, UWK Division Land preparation, planting, thatching, infiling, weeding, fertilizer appli­ cation and infilling of the experimental site were carried out. The experiment is of Randomized Block design with two replicates. The test clones include : TRI 4036,4004,4089,4003,4019,4046,4088,4053,4052,4020,4015,4024 with TRI 2026 as the standard for comparison. The experiment is in progress. S.I. Vitarana, E.St.E.R. Perera and A.K. Premathunga 5.0 Project: C/NEMA Project Leader: Susila I Vitarana 5.1 Analytical services 47 estate samples were analysed during the period under review. 5 let­ ters and 45 reports were sent out. D.D. Liyanage, N. Navaratne, B. Sureshkumar, U.B. Herath and G.P. Udumulla 5.2 In-vitro culturing of nematoes - N288, N289 and N290 Pure cultures of different species of tea nematodes are maintained in the laborataryfor detailed experimental work. Callus tissue of carrot tuber, callus tissue developed from excised root tips of corn and callus tissue developed from excised root tips of tomato are the media for R. Similis, P. loosi and R. reniformis respectively. The method adopted for P. brachyurus is the same as for P. loosi. N. Navaratne and U.B. Herath and Y. Konaratne 7. Project: D/ENTO - Divisional Activities Project Leader: Sushila I Vitarana 7.1.2 Biological Control of Tea Mites Properly designed trials in relation to dosages, times of release, radius of activity and their residual activity were carried out as follows ANNUAL REPORT FOR 1997 81 S.I. Vitarana, S.T. Yatawatte, U.B. Herath, B. Sureshkumar and H.J.M. de Silva E 272 - Lauderdale Estate, Rakwana To study the radius of efficacy of Phytoseiulus persimilis and Amblysius califomicus on oligonychus coffeae. Monthly assessmnets were carried out and the data showed that there is no statistically significant difference between the different radii. It could be inferred that the migratory habits of the two predators are such that they could move to great distances so long as there are no physical barriers such as, ra­ vines, large foot paths, roads and streams. The experiment was terminated. S.I. Vitarana, A.K. Premathunga, E.St.E.R. Perera and H.D. Kumarasena, Superintendent, (Lauderdale - Deveronside Estates, Rakwana) E 273b Lauderdale - Deverenside Estate, Rakwana. To study the effect of Biological Control of Oligonychus coffeae using the predators phytoseiulus persimilis and Amblysius califomicus, on productivity of tea fields during mite outbreak periods. Monthly field vield recorded by the Superintendent in the fields treated with the predators as well as the untreated fields over the period from 1981, were analysed. It was observed that there was a steady upward trend in the yield recorded since the present management took over in 1981. The yield increment was found to be even more after the predatory mites were intro­ duced and since the other agricultural factors remained constant during the period after their release and since there were heavy outbreaks of mites even in 1996 in areas outside the expermental area the additional yield increment could be attributed to the activity of the introduced predators. The data was been referred to the Agricultural Economist for economic analysis. S.I. Vitarana, E.StE.R. Perera, A.K. Premathunga, S.B. Vitana and H.D. Kumarasena, (Superintendent, Lauderdale - Deveronside Estates, Rakwana) 7.2 Chemical Control of Scavenging Termites ME 07 - Duckwari Estate, Rangala Repeat experimentation to corifirm the results of the experiment E268 (where "Admire 200" at 0.50% dilution proved to be the best and effective upto 18 months from treatment) was carried out at the same site using the same plots but, identified under different number, "ME 04". "Admire", is compared as against chlorpyrifos and common salt which are known termiticides. One application of chemical treatments and monthly assessments were carried out. The experiment is in progress. •a 82 ANNUAL REPORT FOR 1997 ME 08 - F. No. - 05C Hagalla Estate, MadulkeUe Chemical Control of Scavenging Termites The following treatments with an untreated control were applied in Au­ gust : Tl - "Admire" 03% solution, T2 -"Admire" 05% solution, T3 - Chlorpyrifos 0.1% olution, t4 - common salt of .0125% solution. All treat­ ments applied as soaking spray at the bottom of the bush covering 1ft up the trunk and a radius of 1ft from the base of the bush. The experiment is in progress. S.I. Vitarana, S.T. Yatawatte, S.B. Abeysinghe, U.B. Herath and H.J.M. de Silva 8.0 Unusual Pest Outbreaks, etc a. There were unusual outbreks of the Fringed Nettle Grub in sev­ eral fields of EI Tebb Estate. Chemical spraying had been carried out from May to end December. Investigations carried out showed that the activity of the parasite population was at a very low ebb. The reasons could not be illucidated. It was decided to monitor the parasite population next and under­ take chemical control if and when natural control factor is assessed to be undependable, so that loss in crop could be minimized. b. Outbreaks of Tea Totrix and tea mites was generaley less than the previous years. One of the contributory factors is the intermittent rains experi­ enced in the pest prone areas during the usual dry seasons. c. Several nurseries were found to have been infested with nema­ todes. In almost all such cases the source of infestation could be traced back to the water supply. Contaminated water was being used either without sedimen­ tation or with ineffective sedimentation mechanisms. In-situ experimentation resulted in a method of eradication of such infestation adopting individual plant application of "Nemacur" granules. The dosages varied according to the de­ gree of infestation. This method would be recommended for eradication of nematodes in infested nurseries in due course. 8.0 Miscellaneous work 8.1 Directory of Tea pests : Comprehensive lists of all tea pests including those of animal origin, plant orgin (pathogens) and also, weed plants, their distribution in the tea pro­ ducing countries in the world, and their bio-control agents occuring in Sri Lanka were complied into one document for the use of the Steering Committee on National Plant Quarantine, Council of Agricultural Research Policy of Sri Lanka, (CARP). Copies of this document are deposited in the institute libraries at Talawakelle and Ratnapura and in the office of CARP. S.I. Vitarana, Lakshmie de silva, A. Balasuriya and G. Prematilake ANNUAL REPORT FOR 1997 83 8.2 Seminars, symposia, Field days and Lectures : Mrs. S.I. Vitarana participated as a member of the Sri Lanka team, at the Technical Committee meeting of the International Standards organization (ISO) Sub Committee on Tea, held in February, in Colombo. Mrs. Vitarana addressed the RSC seminar in Kandy on the topic "Prun­ ing in relation to pest Management", in March. Dr. L.D. Amarasinghe and Mr. K. Thirugnanasuntharan attendd the two day symposium of Entmology working Greoup of the Department of Agricul­ ture held at HORDI, Gannoruwa, in August. Mrs. Vitarana participated at a training programme conducted by the Bureau of Standards on "5S Programme" and delivered a seminar on "Total Quality Management" for the benefit of the institute staff on world standards Day, 14th October. Mrs. S.M. Nagahaulla and Mrs. S.B. Vitana were nominated into a ento­ mological taxonomy group which will function as a national service. They participated at two residential training programmes in Kandy during the course of the year. Dr. L.D. Amarasinghe, Miss. S.M. Samarasinghe, Mrs. S.I. Vitarana and Mr. K. Thirugnasuntharan participated at the biannual meeting of the particpants of the S AREC Biochemical Pest Control Project, at thelnstitute of Fundamen­ tal Studies, Kandy, in July. Entomologist PLANT PATHOLOGY DIVISION Acting Head - A. Balasuriya 1. General A. Balasuriya continued his Ph.D studies at Peradeniya University. Mr T.S. Gunasekara did not return after completing post graduate studies in the U.K. Staffing was at only 50% of the establishment throughout the year. An undergraduate trainee, Ms V. Krishnapillai from the Faculty of Agriculture, University of Jaffna, completed a project entitled, 'Two wood-rot causing fungi in Tea (Camellia sinensis) and some of their biological aspects", spending a little over six months, starting in January. NDT trainees, Ms C.R. Nakandala (NDT, Kuliyapitiya), Mr D. Weeratunge, Ms S. Priyalakshie, Ms K.H.A.D. Kumari and Ms R. Krishnawathany (NDT, Ampara) each completed a four-month training period in the division during the year. Ms H.W. Prabha Kumari and Ms D.G.G. Janakie (NDT, Kuliyapitiya), commenced their training in December. 2. Project: D/PLPA - Divisional Activities Project Leader - A. Balasuriya 2.1. Leaf Diseases 2.1.1. Blister Blight 2.1.1.1. P/BB1/97 - Collaborative studies with advisory staff on the control of the disease. Hantana, Passara and Deniyaya sub-stations were earmarked for this study. In Hantana, the plots were marked and waited for the blister to set in. Since the blisters were not found until early December, this study had to be postponed. 2.1.1.2. P/BB2/97 - Fungicide screening trial, Field No.7, St. Coombs. Altogether, 9 rounds of spraying and 18 Blister Blight assessments were undertaken in this trial, from end July to end November, 1997. The assessments were based on infected third leaf counts of 100 shoots (three leaves and a bud) in each plot. The first four counts did not produce consistent results due to erratic weather patterns. This behavior was continued upto 12th August, one week after spraying the first round. The results of subsequent 15 rounds are presented in Table 1. In this, 14 assessments gave 50% or more control of blisters with all the treatments. All the products compared well with standard Baycor. Both Bumper and RH 7592 at double the concentration gave better control. But during the season since the aim was to maintain blisters below ANNUAL REPORT FOR 1997 85 35% infection level, the lower rates should be sufficient. This will also ensure minimum residue levels in the made tea which is of prime importance. Treatments; 1 .Baycor (BC) 5 ml/101 of water / 85 ml/1701 of water ha 1 2.Calixin (CX) 7 ml/101 of water / 120 ml/1701 of water ha"1 3.Bumper (BM) 6 ml/101 of water / 100 ml/1701 of water ha1 4.Bumper (BM) 12 ml/101 of water / 200 ml/1701 of water ha 1 5.RH 7592 6 ml/101 of water / 100 ml/1701 of water ha 1 6.RH 7592 12 ml/101 of water / 200 ml/1701 of water ha 1 In addition, unsprayed plants from the adjoining area were assessed and appear as the control below. TABLE 1 .Percent shoots with infected third leaf, after treatment with different systemic fungicides in comparison with unsprayed control Date 12/8 22/8 27/8 03/9 10/9 17/9 24/9 01/10 Weeka.s. 1 2 1 2 3* 1 2 1 Baycor 5ml 89.5 48.3 36.7 53.5 33.5 29.5 8.3 18.5 Calixin 7ml 92.2 39.8 49.0 23.8 32.5 31.3 7.3 10.8 Bumper 6ml 85.5 40.0 28.5 33.0 30.8 28.8 9.5 16.3 Bumper 12ml 70.1 18.3 24.0 14.8 31.1 27.5 6.0 5.5 RH 7592 6ml 88.7 24.5 46.8 33.3 24.5 29.5 8.5 17.9 RH 7592 12ml 88.2 18.3 42.1 32.3 18.2 16.5 4.8 11.9 Control 97.0 87.0 94.0 89.0 83.5 69.5 40.0 34.0 Date 08/10 15/10 22/10 29/10 05/11 12/11 19/11 Week a.s. 2 1 2 1 2 1 2 Baycor 5ml 14.5 18.2 12.2 19.0 14.0 18.8 18.8 Calixin 7ml 29.0 31.5 21.3 25.8 15.5 24.8 27.0 Bumper 6ml 22.0 14.8 22.5 16.3 17.7 12.0 11.3 Bumper 12ml 24.3 11.2 7.8 7.8 13.3 11.8 10.5 RH 7592 6ml 18.5 15.0 8.8 34.0 13.8 11.5 13.5 RH 7592 12ml 11.8 16.0 8.5 27.8 10.5 13.0 13.3 Control 59.5 66.8 87.5 50.0 57.0 57.5 75.0 3* weeks after spraying, extended to the 3rd week due to sun-shine Angular Transformed data of the values in Table 1 were used to find the statistical significance of the results of individual treatments (Table 2). Considering Baycor at (85ml/1701) as the standard, both Bumper and RH 7592 at 12 ml/101 of water performed significantly better. By successful control of the disease i.e. maintaining the infections below 35%, it should be possible to achieve a similar level of control by using Calixin at 7 ml, Bumper at 6 ml and RH 7592 at 6 ml per 101 of water, because, these were not significantly different from the standard Baycor. 86 ANNUAL REPORT FOR 1997 TABLE 2. Comparison of two new systemic fungicides with Baycor and Calixin in the control of Blister Blight (Angular Transformed data) (selected dates only) Date 12/8 20/8 10/9 22/10 19/11 Weeks a.s. 1 2 3* 2 2 Baycor 5ml 75.1 43.6 32.8 20.5 25.2 Calixin 7ml 75.0 38.7 33.8 27.0 33.5 Bumper 6ml 68.0 38.9 31.6 28.2 18.9 Bumper 12ml 53.8 25.0 31.6 16.0 18.8 RH 7592 6ml 71.5 29.4 32.7 17.0 21.3 RH 7592 12ml 70.6 25.1 23.7 16.7 20.5 SE(mean) (15) 13.2 26.6 13.3 16.8 23.6 LSD, P<0.05 11.2 11.0 5.1 4.3 6.7 * - 3 weeks after spraying, extended to the 3rd week due to sun-shine A. Balasuriya, D. Pallemulla, N. Karunatillake, and A. Ratnayake 2.1.1.2. P/BB3/97 - Re-evaluation of Cu fungicide dosage rates for the control of Blisters in tea nursery (StCoombs). This study was undertaken from September to November in order to re­ evaluate the dosage rates of Cu fungicides, used in the control of blisters in the nursery. Three groups of the fungicide were used, namely; Oxide, Oxychloride and Hydroxide. The rates were based on current recommendations and the expected solubility of the chemical group. Each group was tested at three appropriate concentrations (Tables 3), on two different clones; TRI 3025 and TRI 4050. This study revealed that all the treatments provided excellent control of the disease when sprayed at weekly intervals. When the infection level in the controls (non-sprayed) was 69%, the maximum recorded in any of the treatments was 8% while the minimum control achieved was 2% (Table 3). Along the three products, CuO (oxide) ranked the best closely followed by Cu(OH) (hydroxide) and CuOCl (oxychloride). Considering different concentrations, CuO at 140g, CuOCl at 90g and Cu(OH) at 80g performed best within each group. ANNUAL REPORT FOR 1997 87 TABLE 3 - Percent infection of plants with blister treated with different concentrations of three copperfungicides, diluted in 451 of water Treatment level of % plants infected infection (transformed date) Control 0.69 0.66 g CuOlOOg 0.08 0.62 b CuO120g 0.06 0.60 b CuO140g 0.02 0.55 cd CuOCl 90g 0.04 0.67 a CuOCl llOg 0.05 0.54 a CuOCl 130g 0.05 0.40 f Cu(OH)2 80g 0.03 0.51 e Cu(OH)2 lOOg 0.03 0.56 c Cu(OH)2120g 0.05 0.004 In the second column, data followed by same letter are not significantly different at P<0.01, by DMRT. A. Balasuriya and N.K.Karunatillake P/BB4 - Control of Blister Blight using house-hold salt, NaCl (St. Coombs - Field 9); Objective: There is speculation that the ordinary salt (NaCl) can be used to control Blister Blight with some success. This is being already practiced by some growers. Our aim was to establish this, and quantify same. NaCl, at three concentrations (1.0,2.5 and 5.0%) were tested along with the standard copper (Perenox). This trial was concluded in 1996 and the percent infection levels with different treatments were discussed in the 1996 Annual Report. It was observed that, NaCl at 5% level, resulted in comparable control of Blister Blight infections similar to Perenox (CuO). The analysis of leaf and soil for residual Na indicated that up to seven rounds of weekly spraying with NaCl (not shown in the Table 4), there was no significant difference among any of the treatments. However, from the 8th round onwards, significant differences were found specially at 2.5 and 5% concentrations of NaCl applications. What is of most significance is the higher levels of Na recorded on mature leaf and soil. Though there are no critical levels of Na established for these parameters, one has to be cautious in resorting to continuous use of NaCl. Since this would allow one to use NaCl up to about seven rounds per season there seem to be some promise in this, as an option to the standard fungicide sprays. 88 ANNUAL REPORT FOR 1997 TABLE 4 - Levels ofNa in leaf(%) and in soil (ppm) after 8, 9 and 10 rounds of spraying (% transformed data) Treatment 14th October 21st October 28th October Flush M.leaf Flush M.leaf Rush M.leaf Soil Perenox 0.262 0.275 0.314 0.254 0.255 0.249 76.3 NaCl 1.0% 0.298 0.310 0.281 0.273 0.281 0.293 82.3 NaCl 2.5% 0.315 0.338 0.299 0.303 0.308 0.365 81.7 NaCl 5.0% 0.352 0.445 0.309 0.315 0.252 0.394 91.0 Mean 0.307 0.342 0.301 0.286 0.274 0.325 82.8 Signific. * NS NS * * ** * LSD P=0.05 0.048 0.038 0.039 0.032 7.0 CV% 7.9 6.7 7.1 5.0 4.2 A. Balasuriya, N.K. Karunatillake and G.P. Gunarathne Efficacy of NaCl (household salt) in the control of Blister spore germination, in-vitro. For a more detailed examination of the mechanism of control by NaCl as treatments, same concentrations of NaCl treatments were used on PDA agar plates to germinate Blister Blight spores. The same four treatments as above were mixed with PDA agar prior to pouring them. Freshly formed blisters were suspended above these plates under a bell-jar. The spore count and the number of spores germinated were recorded respectively 24 and 48 hours after setting up of the experiment. Compared with the control (plain PDA medium), there was only a marginal decrease in the percentage germination of blister spores at 1% NaCl, concentration. NaCl at 2.5 and 5% and Perenox equivalent to standard rates, completely inhibited spore germination (Table 5). This further supports the potential of NaCl to control Blister Blight disease. Table 5 - Spore count and the percent germination of blister spores on the culture plates in response to NaCl and Perenox Treatment Spore count No. germinated Gennination % Control 168 161 96.7 (76-308) (75-308) (92.6-100) NaCl @ 1.0% 496 453 91.1 • (235-833) (219-789) (88.1-96.3) NaCl @ 2.5% 338 0 - (204-581) NaCl @ 5.0% 335 0 - (182-510) Perenox (Cu) 300 0 - @ 0.3% (106-519) A. Balasuriya and N.K. Karunatillake ANNUAL REPORT FOR 1997 89 Stem Diseases - Wood Rot Hypoxylon Wood Rot: P/WRH4 (Nuwara Eliya Estate - Oliphant Division) - Observational experiment on training of the bush frame; Objective: To discourage any shoots arising at or near ground level of the bush, by periodic removel of same, when they are still tender. The aim is to maintain a clearance from ground to the branching off point (neck effect), so that in the event of future infection by Hypoxylon, the total infection could be removed from the bush through what is known as rejuvenation pruning. This is on-going. Several visits were made during the year in order to remove any new basal shoots emerging at the collar region. Connected activities; Ms V. Krishnapillai studied the growth characteristics of Nemania diffusa and Tunstallia aculeata with different C source in the growing medium, with different C:N, C:P, C:K, C:Ca and C:Mg ratios of the medium, at different pH of the medium, at different T of the environment. Biochemical tests were undertaken to assay the kind of enzymes produced by these two fungi. Several samples of live specimens were sent to Washington State University (on personal basis) for the confirmation of the causal organism. This was identified to be Nemania diffusa (Synonym - Hypoxylon vestitum) Some fungal cultures isolated from wood-rot association were identified at the IMI of UK. They were; Fusarium oxysporium, F. solani, Gliocladium roseum, Pestalotiopsis guepinii, Myrothecium roridum, Hymenomycetes sp. Dissemination of disease in the field; This study was undetaken in Diyagama West and Nuwara Eliya Estates with a view to establishing the possibility of spreading the disease through pnining knives in the form of contaminants. The fungus was isolated on PDA medium by using smears (cotton swabs) from the blade of pruning knife. The number of colonies were counted in the Diyagama West estate are presented in Table 6. This showed that the fungus can actually be transmitted through the blade of pruning knives. There were 17 Hypoxylon positive isolates from a total of 18 colonic obtained. 90 ANNUAL REPORT FOR 1997 Table 6 - Results of pruning knife contamination experiment, with Hypoxylon Treatment Total no. of No. of positive Colonies Hypoxoylon colonies Control 3 1 Plate 1 3 3 Plate 2 3 2 Plate 3 3 3 Plate 4 3 3 Plate 5 3 4 Plate 6 3 2 Effect of wood extractives on the growth of N. diffusa and T. aculeata; Repeated the experiment with extractives using four different clones; HS 10, Walaha Yellow (resistant) and PK 2, K 145 (susceptible). They were incorporated to the PDA medium at 50, 100 and 150 ppm for testing growth response of Hypoxylon. The fungus responded with significant differences in response to different treatment combinations. However, no particular trend was seen on account of resistant and susceptible clones. Therefore, it is likely that the biochemical extractives of respective clones are not influencing the susceptibility/resistance of them to Hypoxylon wood rot disease. A. Balasuriya and A. Ratnayake Biological control of Nemania diffusa; Aspergillus niger and a bacterium (contaminant) found to effectively suppress the growth of this fungus very strongly. Presented an abstract paper and a poster at the annual research sessions of the Faculty of Science, University of Peradeniya and at the annual reseach sessions of the University of Peradeniya respectively on the latest findings about Hypoxylon wood rot. General Wood rot P/WRG2 (UH) - Chemical control of Wood Rot using Systemic Fungicides and Protective Paints, at two levels of 'K' fertilizer. Objective; 'K' is known to help in tissue build up of woody plants. This phenomenon is tested here to control the General Wood Rot condition, in combination with systemic fungicides and protective paints. This trial was discontinued after the final assessment of wood rot in January 1997. The following treatments were imposed in this study; ANNUAL REPORT FOR 1997 91 Main Plots M l - Standard rate of'K' M2 - Double the standard rate of 'K' Sub Plots 51 - Tebuconazole (Folicur) spray 52 - Cyproconazole (Atemi) spray 53 - RRI protective paint 54 - Baycor 3PA protective paint 55 - Control (no spraying, no protective painting) Table - 7 Area of wood rot (cm2) patche and their increse (decrease) from 1994 to 1996, under different treatments M 1 M 2 1994 1996 % Diff. 1994 1996 %Diff. s.e.* SI 347 363 1 6 - 463 536 73 22.6 S2 377 330 (47) 359 369 9 S3 333 342 9 349 382 33 S4 291 377 86 384 439 56 S5 372 472 100 339 401 62 s.e.* 35.3 50.1 * the standard errors indicated are only those of the changes in rotted areas from 1994 to 1996. A. Balasuriya, D. Pallemulla and A. Ratnayake The data of this experiment were analysed by coveriance analysis procedure, using 1994 (initial) values as the coveriate. Individual treatments or their interactions did not show any significant effect (Table 7). Since, we had taken extra precaution in cleaning up all the rotted meterial from all individual bushes, one could argue that the through cleaning alone could help in containing general-wood-rot situations when they are in an advanced stage. This field had been planted with seedlings in the year 1922 and therefore, approximately 75 years old. As a result we had to depend on highly variable wood rotted patches as they occurred naturally. This invariably resulted in high CV values (44.0 -51.1%). This may have obscured some treatment effects. However, according to the third column (Table 7) the highest percent increase (46.5%) in the area due to wood rot was with MlxS4 and the least (1.6%) with MlxS3. When the individual treatments were considered the best performance (an actual reduction) was seen with S2 (Cyproconazole spray) closely followed by S3 (RRI protective paint.) 92 ANNUAL REPORT FOR 1997 A protective paint such as Baycor 3PA, has better lasting properties. Therefore, when partially cleaned wounds are painted with a similar paint there is a possibility that some of the microorganisms responsible too get protected within the infected woody material. Well-protected from outside agencies such organisms may be able to do more damage. Therefore the highest amount of damage was observed with Baycor 3Pa treatment. On the other hand a paint like RRI-bitumen mixture which does not last equally well, must be only protecting the wound initially from the elements of environment. With passage of time they develop cracks which would not help in securing a profitable ecological niche for the proliferation of the microbial activity. While this is happening the individual bushes too support the healing process with their inherent capacities to callus over and repair (as seen in this experiment) some of the damage. Arguably, Cyproconazole spraying could be effective in containing general wood rot to some extent. Low Country Stem Canker P/CANK1/97 (St Joachim) - Screening line for resistance in lowcountry stem canker in the plant breeding trials. This series of evaluations were done on 584 lines in 1113 replicates. The number of plants in each replicate was variable being 5, 7 and 10. The following key was used in assessing the degree of severity of canker in each individual clone; 0 - No sign of cankers 1 - One or two small cankers 2 - Several small cankers 3 - Few large cankers 4 - Cankers spreading to the base of plant 5 - Dead due to canker The values obtained, were adjusted for the following considerations. a. For me healing capability of canker wounds; (Scores of 1,2, 3 & 4 multiply by 0.6, 0.7,0.8 & 0.9 respectively) b. For the number of plants per plot; (Score multiply by 5 (mimmum plot size/actual no. of plants per plot) c. For the number of replicates per line/clone; (For replicate number 1, 2, 3, & 4 multiply by 1.0, 0.9, 0.8 & 0.7 respectively) ANNUAL REPORT FOR 1997 93 Table 8 - Ranking and their respective numbers of lines / clones for the resistance / susceptibility to low country stem canker. Series No. of No. of No. of lines in each rank Identification plots lines 1 2 3 4 5 LVP-61 Parambe, 11-12 21 12 - 2 3 7 Parambe, J1-J2 20 12 5 7 - - LVP - 62 St. Joachim, F1-F2 34 17 - 3 9 5 St. Joachim, H1-H2 14 7 1 - 2 4 St. Joachim, X1-X2 14 7 - 3 3 1 Non-replicated 7 7 - 1 - 6 Balance 29 29 2 2 17 6 2 LVP-63 Pettigala, A3-A4 110 55 1 6 14 19 15 Pettigala, B1-B2 26 13 4 6 1 2 Pettigala, E1-E2 106 53 - 1 11 41 LVP-66 161 83 - 20 39 24 LVP-67 209 105 4 28 32 41 LVP-68 149 76 - 19 32 26 LVP-71 89 45 3 17 23 2 - LVP-72 124 62 5 16 28 6 7 Total 1113 584 11 55 172 165 181 Percentage 2% 9% 30% 28% 31% A. Balasuriya, D. Pallemulla and A. Ratnayake Based on above adjusted averages (final disease scores) individual lines/clones were ranked for their resistance/susceptibility to canker disease as follows, Disease score of: 0-0 .99 - Extremely resistant 1-1.99 - Resistant. 2 - 2 99 - Moderately susceptible 3 - 3.99 - Susceptible 4 and above - Extremely susceptible Accordingly, out of 584 lines examined (in 1113 plots), there were 11 (2%) of them showing very good resistance anf 55 (9%) showing moderate susceptibility to low country stem canker disease (Table 8). 94 ANNUAL REPORT FOR 1997 P/CANK2/97 (St Joachim) - Use of systemic fungicide sprays for the control of low-country-stem-canker. This trial was started in St. Joachim estate, using a clonal multiplication area. Two TRI clones. 4047 and 4049 and two systemic fungicides, Baycor and Calixin at the rate of 0. .1% solution were used as treatments. After an initial assessment on the intensity of canker, two applications of fungicides were effected at two monthly intervals. Final assessment is due after two more applications of same fungicides.. SHB - Ambrosia control studies P/AMB3 (Passara sub station) - Efficacy of systemic fungicides as an indirect measure of controlling SHB damage Treatment& consisted of three rounds of spraying/drencliing, followed by population counts through destructive sampling. The treatments were all combinations of: fungicide : Folicur vs Contaf Rates : 0. .05%, 0. .5% vs 5. 0% Method of application : foliar spray vs root drenching plus a control Design: Randomized Complete Block (RCBD),. two replicates, five plants per plot. This trial was completed in the first quarter and the results are given in Table 9. Similar to previous assessments there were significant effects on the recoverey of Ambrosia fungus on culture plate. Contaf (Hexaconazole) at 5.0% as a root drench being superior to the rest of the treatments. However this was not followed by any of the SHB counts.. A similar experiment with more number of fungicide applications was repeated at Hantana sub station on the request of JPM task force for the control of SHB. ANNUAL REPORT FOR 1997 95 TABLE 9 - Response or the Ambrosia fungus on the culture medium and the SHB beetles to different systemic fungicide Treatments Ambrosia score SHB beetle counts Total No. galleries Original sqrt young new live 1 9 3.01 11 12 18 12 2 14 3.74 14 14 22 41 3 6 2.41 6 7 12 10 4 3 1.87 27 27 39 13 5 14 3.81 14 13 23 12 6 7 2.64 1 1 4 8 7 5- 2.21 10 9 15 12 8 11 3.27 13 17 26 10 9 9 2.90 11 10 17 8 10 5 2.23 17 16 22 10 11 10 3.16 19 13 27 3 12 4 2.07 11 9 18 9 13 0 0.71 19 11 26 13 Mean 7 2.62 14 12 20 13 Signif.. * ns ns ns ns LSD (P=.05) 1.38 CV% 24.: * transformed data square root (x+.5) Die-back in K145 A repeat study to confirm previous findings was initiated towards the end of the Year Biological control of cooch grass Identified a fungus causing a smut disease in. Panicum repens, to be Ustilago digitariae. Steps were taken to investigate the possibility of using this phenomenon to control this problem weed in future.. Mycorrhiza Written two articles for the Sri Lanka Journal of Tea Science with the data collected from recently concluded two trials. They are. EFFECT OF APPLICATION OF NPK FERTILIZERS ON THE NATURAL INCIDENCE OF VESICULAR ARBUSCULAR MYCORRHIZA IN THE RHIZIOSHEREE SOILS AND FEEDER ROOTS OF TEA (CAMELLIA) SINENSIS)' and INFLUENCE OF MYCORRHIZA, SYSTEMIC FUNGICIDE (TEBUCONAZOLE) AND ADDED E' ON PHOSPOROUS UTILIZATION BY TEA (CAMELLIA SINENSIS)'. 96 ANNUAL REPORT FOR 1997 High Forest Problem Dr. Mike Deadrman of Reading University , during his visit shared our view of an involvement of a bacterium as a possible cause for the problem. A confirmation on this is awaited. This same problem was observed for the first time on Frotoft estate, Pussellawa on clone TRI 2025, about the same age. New Problems In Pedro Estate, a new problem of stem galling was found to be associated with the debilitated old seedling bushes. Sample were sent to UK, through courtesy, Dr Milk Deadman who was shown this problem. Preliminary investigations revealed it to be an ascomy fungus. Further identification is awaited. Leaf variegations on young, old and recovering bushes were reported from various parts of Up Country and Uva Estates. In Pedro estate it was possible to monitor this situation for period of time, and was found to disappear with the rains. This condition is normally associated with dry weather and therefore suspected to be influenced by high (UV) radiation. TRI Update Published two communications in the TRI Update. First of this was 'WOOD ROTS - THORNY STEM BLIGHT OR HYPOXYLON ?," drawing attention of the estates concerned to the latest findings of this division and the second on 'WHY, RING-BARK SHADE TREES?' Wghlighting the salient features of this very useful operation- Collaborative Research Corresponded with several estates on proposed collaborative research activities to be undertaken shortly. A selection experiment for the formation of callus was already started in Hapugastemme Estate, Maskeliya. With Frotoft Estate,, preliminary discussions were held and a proposal prepared for the Superintendent's comments. Discussions Had a useful discussion on Blister Blight disease of tea with Dr E. Hamaya of Japan, when he visited Hantana. Mr Balasuriya also took part in a discussion with the CEO's of the Plantation Management Companies in Colombo by invitation. ANNUAL REPORT FOR 1997 97 Seminars / Lectures Mr Balasuriya addressed the RSC-IV,, Kandy, seminar on 'Pruning in relation to Wood Rot and Blister Blight, and RSC-I,. on the 'Control and Management of Blister Blight Disease', at the TRI auditorium. Mr Balasuriya also participated in RSC-I seminar on tea manufacture.. Participated in a seminar , conducted by Messrs Hiran Weerasekara (Harrisons), Upali Gangoda ((Heychem)) and Keerthi Liyanage (Finchem) on the safe handling of pesticides. Mr Balasuriya addressed the 6th Plant pathologists work group meeting of the Department of Agriculture, held at Mahaillupplama All the staff of the division attended a series of lectures organised by the TRI. on ISO 9000 Visitors Dr Mike Deadman of Reading University, UK, visited the division followed by a visit to the fields in High Forest and Pedro estates. The second year students of the Faculty of Agriculture (Peradeniya) visited the division on two days in four batches A group of 38, B. Sc Agriculture undergraduates from the Sabaragamuawa University and also a group of students from Agriculture school in Pelwehera visited the division. Capt. Migara Zoysa of Agalawatte Plantations spent a day to refresh his knowledge on tea disease control. Dr Kohler of Bayer, accompanied by Mr. Upali Gangoda of Heychem visited the division. Five trainee Assistant Superintendents underwent training on the diagnosis and the controlling of important tea diseases The following advisory visits were undertaken on the request of respective advisory officers in those regions: Dickwella and St. James estates in Hali Ela. on death of pruned bushes Several small holders in Kottawa, on Horse hair blight disease. Loinorn estate, on death of OST bushes due to Thorny Stem Blight Pedro estate on stem galls and leaf variegation problems. Wattegoda estate on a fairly heavy Phomopsis canker incidence. Small holder in Passara on Phomopsis canker disease. Campion estate on Hypoxylon wood rot incidence. Mountjean estate on a bad attack of Poria-root disease. Hapugastenne in Maskellya to start selecting from OST for Blister Blight resistance. Wewessa, Rookwood and Gonakelle estates, on non recovery of bushes and bush debilitation. Frotoft estate on a 98 ANNUAL REPORT FOR 1997 large area of non recovering tea bushes after pruning which was apparently a physiological problem, Two small holder properties in Sooriyagoda, on poor performance of certain clones (TRI 2023 ) and on Phomopsis canker . Acknowledgements The co-operation and the ready assistance extended to us by all estates during our numerous visits to their properties and particularly where the divisional experiments and detailed studies were undertaken, is gratefully acknowledged. PLANT PHYSIOLOGY DIVISION Acting Head - A.Anandacoomaraswamy 1. General Ms.D.M.S.Navaratne completed the B.Sc examination conducted by the University of Peradeniya. Ms J.Mohotti returned to Rothamstead Experimental Station (UK) in July to complete the Ph.D.program.The following students from Faculty of Agriculture, of theUniversity of Peradeniya completed their final year projects in the division: Ms Shammalie Hewasinghe, Mr.D.K.C.Perera, Ms. P.Tennakoon, Ms L.Priyantha and Ms. Anjalika Balasingham. 2. Studies on Photosynthesis and Dry Matter Partitioning 2.1. Partitioning of assimilates in relation to aging of clonal tea in a pruning cycle, St Coombs Estate - (1992) The annual yield of clones TRI 2025 and DTI with age is presented in Table 1. TABLE 1 - Effect of age on yield(kg her1) Clone Year from Prune 2 3 4 7 LSD(P=0.05) CV % 15 8 The seventh year yield was from January to April. There were no 1st year plants in the current year. Yield difference from the 2nd to the 4th year was not significant in both clones. V.Sithakaran 2.2. Effect of resting before pruning on root starch, TRI 2023 New Peacock Estate - (1995) The yield for the different treatments are presented in Table 2. TABLE 2 - Yield(kg ha1) Treatments Yield(kg ha1) One month resting 2006 Two month resting 2061 TRI 2025 DTI 2435 2488 2823 2367 2722 2175 542 382 440 201 100 ANNUAL REPORT FOR 1997 There was no susbstantial difference in yield between the treatments probably due to the adequate root starch levels at the time of pruning. A.Anandacoomaraswamy and D.M.S.Navaratne 2.4. Resting before pruning and root starch accumulation in tea (K145), Mattakelle Estate(Observation Trial) The plots were brought into plucking in Octber 96. The yield data for the first year is presented in Table 3. TABLE 3 - Yield(kg ha1) Treatments Yieldfkg ha') One month resting 2364 Two month resting 2348 Yield marginally improved due to resting A.Anandacoomaraswamy and D.M.S.Navaratne 2.5. Effect of levels of nitrogen on partitioning of assimilates in nursery plants (TRI 2025). Three levels of nitrogen (0, 52.5 and 105ppm) in Hewitt solution were tried. The Pmax, light use efficency and assimilates partitioning were determined. The critical level of leaf nitrogen was 2.9%. P.Tennekoon 2.6. C 1 4 Studies Scintillation counter was repaired only in August. However, there were no studies conducted under this project. V.Shanmugarajah 3. Tea Physiology and Potassium Nutrition 3.1. Effect of potassium on recovery from pruning, St Coombs Estate - (1993) This experiment is in the final year of the current cycle. The yield is given in table 4. ANNUAL REPORT FOR 1997 101 TABLE 4 - Yield(kg ha') Main Treatments Sub treatments Yield (a) Pre - prune 2:1 2404 1:1 2460 1:2 2598 1:3 2911 (b) Post - prune 2:1 2592 1:1 2746 1:2 2461 1:3 2590 (c) Pre and Post-prune 2:1 2448 1:1 2472 1:2 2612 1:3 2729 LSD(P=0.05) NS CV % 9 There was no significant difference in yield between the time of fertilzer application and different N:K ratios. V.Sithakaran 3.2. Potassium nutrition on physiology of young tea (TRI 2025, DTI) Both clones had completed 12 months in the nursery. Photosynthesis and growth measurements were taken. Growth measurements are presented in Table 5. TABLE 5 - Growth measurements Treatments Height No of side Leaf shoots Area (cm) (cm) Clone TRI 2025 1. Control(No potassium) 41.2 5 486 2. Recommended level of K 42.0 4 587 3. l/4th of recommended level of K 31.6 3 610 4. 1/2 the recommended level of K 41.3 5 628 5. Double the recommended level of K 44.8 5 507 6. Treble the recommended level of K 46.8 6 769 Treatments Height No of side Leaf shoots Area (cm) (cm) Clone DTI 1. Control(No potassium) 38.6 4 343 2. Recommended level of K 46.1 3 495 3. l/4th of recommended level of K 43.9 5 385 4. 1/2 the recommended level of K 50.2 5 431 5. Double the recommended level of K 43.4 5 434 6. Treble the recommended level of K 48.2 6 508 102 ANNUAL REPORT FOR 1997 Clone TRI 2025 Clone TRI 2025 showed better growth with respect to height, number of leaves produced, total leaf area and number of shoots produced at the highest level of potassium. Clone DTI Except for the height, plants of clone DTI also showed better growth at the highest level of potassium. V. Shanmugarajah 4. Drought Mitigtion 4.1. Drought mitigation in mature tea, (TRI 2025)- St Coombs Estate, (1995). The effect of spraying 'Green Miracle' - an antitranspirant on yield was evaluated in the mature tea plants. The yield is presented in Table 6. TABLE 6 - Yieldfkg ha') Treatments Yield 1. Control(Water spray) 5417 2. Green Miracle(l%) 5555 3. Green Miracle(l%) + Potassium chloride (1 %) + Urea(l%) 5063 4. Potassium chloride (1%) + Urea(l%) 4986 LSD(P=0.05) 463 CV% 5 The effect of 'Green miracle' on yield was not pronounced probabaly due to the mild drought experienced during the early part of the year. The results also indicate that incorporation of potassium chloride in the spray solution reduced the yield. A.Anandacoomaraswamy and V.Sithakaran 5. Effect of Retention of Crow's Feet (Mudichchis) on Yield of Tea (TRI 2025)- St Coombs Estate This experiment was repeated during the current year and the yield for the period from July - December is given in Table 7. ANNUAL REPORT FOR 1997 103 TABLE 7 - Yield(kg ha1) Treatments Yield 1. Retention of Crow's feet 1158 2. Removal of Crow's feet 1168 LSD(P=0.05) NS CV(%) 16 There was no significant difference in yield between the treatments indicating the removal of crow's feet does not improve the yield. A.Anandacoomaraswamy, A.Kathiravetpillai and V.Sithakaran 6. Modeling Tea yield from Weather Data Intensive weather measurements using automatic weather sensors continued at St Coombs Estate, Court Lodge Estate and Uva High lands Estate. A tea model was built using MACROS at the Institute for Meteorology and Physics, University of Agricultural Sciences BOKU, Austria. A.Anandacoomarasamy, Josef EITZINGER and D.M.S.Navaratne 7. Effect of Canopy Height on yield of Tea TRI 2025 - St Coombs Estate An experiment was initiated to investigate the effect of increasing canopy height on the yield of tea in mature plants. These plants were in the fourth year of the current cycle. An average height difference of 18 cm was maintained between the two canopies. The yield is presented in Table 8. TABLE 8. Yield (kg/ha) Treatments Yield 1.Canopy height of 135 cm 2606 2. Canopy height of 153 cm 2247 LSD (P= 0.05) 303 CV% 12 A.Anandacoomaraswamy and V.Sithakaran 8. Physiological and Morphological parameters associated with yield improvement in clonal tea in Up-country A study was undertaken to identify the important physiological and morphological parameters related to the yield improvement of up-country tea 104 ANNUAL REPORT FOR 1997 clones originated at different times. Clones used in this study were DTI, B 275 (1950's), TRI 2025 (1960's), TRI3013 (1970's) and TRI4052 (1980's). Physiological parameters- Pmax (Maximum net assimilation rate), Efficiency of Photosystem II (Fv/Fm) and water use efficency showed positive correlation with the yield. Morphological parameters - specific leaf weight and internode length also showed similar correlation with the yield. The results suggest that these traits could be used in the nursery stage to screen the clones for yield improvement. A.Anandacoomaraswamy, S.P.Nissanka and D.K.C. Perera 9. Seminars/Lectures 1. A. Anandacoomaraswamy presented a paper on the 'Spacing of wind belts in tea plantations' at the E & E forum held on 23rd January 1997 2. A. Anandacoomarasamy presented a review paper on 'Water use in Tea Plantations' at a workshop organised by Atomic Energy Authority held on September 5th in Colombo. 10. Publications Eitzinger J and A.Anandacoomaraswamy (1997). Modelling the growth and Yield of Tea In the proceedings ofthe Second International Symposium on Mathematical modelling and Simulation in Agricultural and Bio- Industries(Edited by Prof. I.Farkas) organised by Department of Physics and Process Control Godollo University of Agricultural Science, Budapest, Hungary. PLANT PROPAGATION AND PLANT BREEDING DIVISION Officer-in-charge - V.Shanmugarajah 1. General Mr P.D. Upali was transferred back to Hantane with effect from February 1. Ms S.K. Sathyapala resumed duties after successfully completing her Ph.D. programme at the Miyazaki University, Japan on March 31. Mr M. Ratnayake was awarded a Netherlands fellowship and followed the 27th International Course on Applied Plant Breeding from March 10 to June 21. Mr H. Jayaweera, Experimental Officer of the Biochemistry Division was transferred to the Tissue Culture Unit of this Division and he assumed duties at the Tissue Culture Unit at Hantane with effect from May 2. Mrs M.T.K. Gunasekara resumed duties after successfully completing her Ph.D. programme at Southampton University, UK on December 22. Miss T. Kathiresampillai, a postgraduate student from the Botany Department of the University of Colombo carried out her project work on anther culture. Mr R. Paskarathevan, a graduate trainee continued his work on micropropagation. Miss S. Dhason, an undergraduate student from the Botany Department, had her vacation training. Three NDT students from the Kuliyapitiya Technical College completed their training and one from Hardy Senior Technical Institute commenced his training in this Division. 2. Project A/CLON - The Development of New Clones Project leader - V.Shanmugarajah 2.1. Polyclonal/Biclonal seed 2.1.1. VP 52 - Evaluation of polyclonal seed from the seed gardens at Karandupona and Urumiwella - Field No.9 St. Coombs Estate, Talawakele- (1990) and VP 58 - Evaluation of polyclonal seed - Field No. 9, StCoombs Estate, Talawakele -(1991) Refer 4.2.13 and 4.2.14 under Phase I. 106 ANNUAL REPORT FOR 1997 2.1.2. VP 64 - Evaluation of polyclonal seed - Venture Estate Norwood (1992) Bushes are plucked by the estate, and due for selection in 1998. 2.1.3. VP 65 - Evaluation of polyclonal seed - Carolina Estate, Watawala - (1992) Cuttings from 117 selected bushes have been propagated in the TRI nursery for further evaluation. 2.1.4. VP 66 - Evaluation of polyclonal seed. Luckyland Estate, Udapussellawa - (1992) Bushes have been pruned. 2.1.5. VP 78 - Evaluation of Biclonal and polyclonal seed from El-Teb, Halpe and StCoombs Estates in Field No. 12, St. Coombs Estate, Talawakele (1996) Most of the seedlings planted died during the drought and the vacancies have been filled with clone TRI 3072. V. Shanmugarajah, M. Ratnayake, S.W. Gunadasa and B.A. Rathnaoda 2.1.6. LVP 49 - Evaluation of polyclonal seed from Karandupona and Urumiwella seed gardens. St. Joachim Estate, Ratnapura - (1991) Refer 4.1.15 and 4.2.15 under Phase I. 2.1.7. LVP 53 - Evaluation of polyclonal seed on Hapugastenne estate - (1991) Bushes are plucked by the estate. 2.1.8 LVP 69 - Evaluation of polyclonal seed from Karandupona and Urumiwela seed gardens in field No. 2A, St. Joachim Estate, Ratnapura - (1994) and Biclonal (DN x 2025) seeds from Hugoland seed garden in field No. 2A, St. Joachim Estate, Ratnapura - (1994) ANNUAL REPORT FOR 1997 101 Parentage Yield K 2023 (polyclonal) 2701 K DG 39 (polyclonal) 1706 K 2016 (polyclonal) 1590 2025 x DN 1517 K 2021 (polyclonal) 1470 2025 x DN 1412 K DN (polyclonal) 1376 K 2025 (polyclonal) 1253 A.K.M. Jayasena, J.H.N. Piyasundara, M. Ratnayake, S.W. Gunadasa and V. Shanmugarajah 2.1.9. VP 74 - Evaluation of biclonal seed from El-teb seed garden in Uva (DN x 2025) and polyclonal seed from Sapumalkande seed garden in Field No. 14, St. Coombs Estate, Talawakele - (1995) Plants have been allowed to grow freely. M. Ratnayake, S.W. Gunadasa, B.A. Rathnagoda, S. Umah and V. Shanmugarajah 3. Controlled Hybridisation Experiments 1. Crosses of 1980/1981 (see VP 39) 2. Crosses of 1982/1983 (see VP 43) 3. Crosses of 1984 (see VP 44) 4. Crosses of 1985/1986 (see VP 45) 3.1. VP 39 - Evaluation of seedlings obtained from the crosses of 1980/1981 See VP 67, VP 70, VP 71, LVP 66 and LVP 72 (4.2.6,4.2.8,4.2.9,4.1.10 and 4.1.14 respectively under Phase I) 3.2. VP 43 - Evaluation of seedlings obtained from the crosses of 1982/1983 See VP 72 and LVP 67 (4.2.10 and 4.1.11 respectively under Phase I) 3.3. VP 44 - Evaluation of seedlings obtained from the crosses of 1984 See VP 73 and LVP 68 (4.2.11 and 4.1.12 respectively under Phase I) 3.4. VP 45 - Evaluation of seedlings from the crosses of 1985 See VP 75 (4.2.12 under Phase I) Yields of the seedling progenies of different sources, planted separately in blocks, are given in Table 1. TABLE 1 - Yields of the seedling progenies (kg ha 1 an 1) 108 ANNUAL REPORT FOR 1997 General Interspecific crosses between Camellia sasanqua (seedlings) and diploids and diploids of Camellia sinensis were carried out. The number of flowers of C. sasanqua crossed with pollen from clones of C. sinensis and the percent fruit set are given below: C. Sasanqua fruit set per cent TRI 777 48 0.0 DT 1 29 10.3 HS10A (triploid) 160 7.5 TRI 2025 32 15.6 When Camellia sasanqua was used as the male parent and crossed with 230 flowers of clone HS 10A (triploid), the fruit set was only 0.86 %. Self Pollination To obtain pure lines of some popular clones the flowers were bagged and allowed to setf-pollinate naturally, but no fruits were obtained. The clones and the number of flowers allowed to self are given below: Clone No. of flowers TRI 2024 103 TRI 2025 10 TRI 777 50 TRI 2043 50 DT 95 75 TRI 2142 10 ASM 4/10 20 S. Umah, B.A. Rathnagoda and V. Shanmugarajah 4. Testing of clonal progenies (Phase 1) 4.1. Low country 4.1.1. LVP 55 (LVP 30/LC/T)-Evaluation of clones from Aislaby and Hugoland seed (LVP 30, Deniyaya -1983) on Handford Estate, Deniyaya - (1991) Refer 5.2.3.under Phase U. 4.1.2. LVP 56 (VP 37/LC/l)- Evaluation of clones from VP 37 (St. Coombs) in Field No. 5, St. Joachim Estate, Ratnapura (1992) ANNUAL REPORT FOR 1997 109 4.1.3. LVP 57 (LVP 42/LC/l) - Evaluation of clones from polyclonal seed established on Parambe (LVP 42), in Field No. 5 St. Joachim Estate, Ratnapura (1992) 4.1.4. LVP 58 (LVP 28/LC/l)- Evaluation of clones from Aislaby seed (LVP 28,1983 StJoachim Estate) in Field No. 5, St. Joachim Estate, Ratnapura (1992) 4.1.5. LVP 59 -Evaluation of clones developed from biclonal seed (2026 x DN) established on Pettigala Estate in Field No. 5, St. Joachim Estate, Ratnapura (1992) Promising clones of LVP 56,57,58 and 59 were propagated and a Phase II trial established (Refer 5.2.4. under Phase II). A.K.M. Jayasena, J.H.N. Piyasundara, S.W. Gunadasa, M. Ratnayake and V. Shanmugarajah 4.1.6. LVP 61 (LVP42/LC/I)- Evaluation of clones from polyclonal seed established on Parambe (LVP 42), in Fields No. 2A & 5N St. Joachim Estate, Ratnapura (1993) The yields of the 20 highest yielding clones along with control clone TRI 2023 are given in Table 2. The number of clones tested were 50. TABLE 2 - Yield of clones (Kg MT ha-1 an-1) Clone Yield 186 7514 160 6624 243 5951 253 5883 281 5864 285 5585 375 5464 342 5320 171 5226 168 5213 289 5080 298 5003 109 4996 119 4858 322 4786 10 4752 173 4614 169 4556 110 ANNUAL REPORT FOR 1997 107 66 4481 4446 4437 1202 3330 2023 (control) SE (1 mean) LSD (P=0.05) C V % 29.7 4.1.7. LVP 62 (LVP 45 & LVP46/LC/T)- Evaluation of clones from polyclonal seed established on StJoacliim Estate (LVP 45,1989 & LVP 46,1990), in Field No. 2A and 5N St. Joachim Estate, Ratnapura (1993) Of the 72 clones tested, seventeen clones yielded more than the control clone TRI 2023 and the yields are given in Table 3. TABLE 3 - Yield of clones (Kg MT ha-1 an-1) Clone Yield 63 5516 11 4426 59 4349 47 4090 12 3855 2025 (control) 3715 13 3692 64 3629 23 3624 49 3607 52 3565 54 3416 51 3294 55 3153 72 3037 36 2945 2027 (control) 2715 58 2703 10 2668 2023 (control) 2330 67 2169 70 2147 53 1934 30 1386 SE(mean) 1102 LSD (P=0.05) 3053 C V % 40.3 ANNUAL REPORT FOR 1997 1U 4.1.8 LVP 63 - Evaluation of clones developed from biclonal seed (2026 x DN) established on Pettigala Estate in Field No. 2A & 5N, St. Joachim Estate, Ratnapura (1993) The highest yielding clones along with the controls are given in Table 4. The number of clones tested were 52. TABLE 4 - Yield of clones (Kg MT ha-1 an-1) Clone Yield 19/12 10397 4/2 9106 21/23 8744 2023 (control) 6836 8/9 6293 30/7 6012 19/1 5926 1/2 5756 8/8 5654 2025 (control) 5545 14/17 5477 29/22 5409 31/1 5409 11/11 5387 25/36 5193 23/16 5174 23/22 5173 15/18 5131 14/19 5097 2027 (control) 4883 SE (mean) 1213 LSD (P=0.05) 3556 C V % 32.4 4.1.9. LVP 64 (ASM.INT.88/LC/T) - Evaluation of clones from ASM 1988 Introduction seed in Field No. 2A, St. Joachim Estate, Ratnapura (1993) The yields of the 8 clones tested, along with control clones over 33 plucks are given in Table 5. 112 ANNUAL REPORT FOR 1997 TABLE 5 - Yield of clones (Kg MT ha-1 an-1) Clone Yield 11 5 5074 4521 4313 4200 4193 3899 3228 2877 2533 2344 1336 333 1049 2023 (control) 10 12 2025 (control) 6 19 18 16 2027 (control) SE (mean) LSD (P=0.05) cv% 9.82 4.1.10. LVP 66 (VP 39/LC/I)- Evaluation of clones from VP 39 in Field No.2A, St.Joachim Estate - (1994) Yields of the seven highest yielding clones out of 78 tested, along with those of the controls are given in Table 6. TABLE 6 - Yield of clones (Kg MT ha-1 an-1) Clone Yield 2026 (control) 6478 2023 (control) 6016 113 5665 267 5115 95 4847 129 4665 33 4431 38 4292 222 4123 2027 (control) 4100 SE (mean) 1005 LSD (P=0.05) 2786 C V % 33.0 4.1.11. LVP 67 (VP 43/LC/T)- Evaluation of clones from VP 43 in Field No. 2A, St.Joachim Estate, Ratnapura (1994) Of the 106 clones tested, yields of thirty one high yielding clones along with the control clones are given in Table 7. ANNUAL REPORT FOR 1997 113 TABLE 7 - Yield of clones (Kg MT ha-1 an-1) Clone Yield 814 7301 2023 (control) 6003 710 710 5032 571 4539 759 4480 655 4440 708 4399 2025 (control) 4367 679 4363 626 4254 757 4095 709 3996 554 3959 628 3943 840 3935 769 3932 685 3896 627 3828 621 3787 661 3749 612 3728 657 3706 624 3683 588 3624 770 3588 768 3520 504 3463 763 3434 557 3368 745 3348 702 3339 735 3262 744 3255 2027 (control) 3239 SE (mean) 1447 LSD (P=0.05) 4009 C V % 46.3 4.1.12. LVP 68 (LVP 44/LC/T)- Evaluation of clones from VP 44 in Field No.2A, StJoachim Estate (1994) Yields of the eleven highest yielding clones along with the control clones are given in Table 8. The number of clones tested were 74. 114 ANNUAL REPORT FOR 1997 TABLE 8 - Yield of clones (Kg MT ha-1 an-1) Clone Yield 1021 5323 917 4639 2026 (control) 4331 930 4213 1034 3959 970 3958 959 3919 950 3878 935 3753 2023 (control) 3660 2025 (control) 3599 1037 3516 912 3331 952 3236 2027 (control) 3082 SE (mean) 499 LSD (P=0.05) 1381 cv% 18.8 4.1.13. LVP 71 (LVP 45 and LVP 46/LC/T) - Evaluation of clones from polyclonal seed LVP 45 and LVP 46, in Fields Nos. 2A & 5N, St. Joachim Estate, Ratnapura (1994) Yields of the fifteen highest yielding clones out of 45 tested, along with those of control clones, are given in Table 9. TABLE 9 - Yield of clones (Kg ha-1 an-1) Clone Yield 3/12 6011 6/28 5619 11/46 5605 4/40 5518 15/40 5379 4/16 5270 14/52 5181 5/38 5025 1/9 4964 6/38 4962 14/51 4790 5/35 4651 6/2 4610 7/33 4593 ANNUAL REPORT FOR 1997 115 2/13 4549 2026 (control) 4514 6/23 4486 13/30 4423 4/4 4371 15/51 4354 4/21 4334 6/24 4281 5/34 4270 3/38 4196 6/13 4092 3/11 4018 6/14 3932 3/15 3774 5/21 3731 6/36 3721 15/48 3706 6/29 3666 8/75 3567 3/2 3556 2/4 3375 6/32 3172 2027 (control) 3053 SE (mean) 746 LSD (P=0.05) 2066 C V % 20.1 4.1.14. LVP 72 (VP 39/LC/D- Evaluation of clones from VP 39 in Field No.2A, StJoachim Estate, Ratnapura - (1994) Yields of fourteen high yielding clones along with the controls are given in Table 10. The number of clones tested were 62. TABLE 10 - Yield of clones (Kg MT ha-1 an-1) Clone Yield 232 3712 122 3448 34 3321 52 3285 44 3244 2023 (control) 3076 76 2900 20 2843 112 2814 57 2776 181 2517 116 ANNUAL REPORT FOR 1997 2025 (control) 2403 2387 2306 2283 2217 2172 261 273 113 188 2027 (control) V. Shanmugarajah, A.K.M. Jayasena, J.N. Piyasundara, M. Ratnayake, S. Umah and S.W. Gunadasa 4.1.15. LVP 73 (LVP 49/LC/I) - Evaluation of clonal selections from Urumiwela and Karandupona (LVP 49) in Field No. 1, St Joachim Estate, Ratnapura (1996) The plants were centred twice at 30 and 40 cm (12 and 16 inches) in June and August and the fresh weight of the shoots removed were recorded. 4.2. Upcountry 4.2.1. VP 49 (VP 37/UC/T)- Evaluation of clones from VP 37 (St. Coombs) in I^amiliere Division, StCoombs (1990) Refer 5.1.3 under Phase II. 4.2.2. VP 55 and VP 56 (VP 38/UC/T) - Evaluation of clonal selections from Aislaby, Hugoland and Hantane seed (VP38) in Field No.9, St. Coombs Estate, Talawakele (1991) Cuttings of 30 promising selections have been propagated in the nursery for a Phase II trial. 4.2.3. VP 60 (VP 37/UC/T) - Evaluation of clones from VP 37 in Field No. 10 St. Coombs (1992) Bushes have been pruned for propagation. 4.2.4. VP 61 (ASM.INT.88/UC.1/I) - Evaluation of clones from ASM 1988 Introduced Seed. Field No. 10. St. Coombs Estate, Talawakele (1992) Bushes have been pruned for propagation. 4.2.5. VP 63 (VP 37/UC-2.NOR/I) - Evaluation of clones developed from VP 37 and from Aislaby seed. Venture estate, Norwood (1992) The bushes were pruned, weight of prunings recorded and cuttings have been propagated in the TRI and Norwood estate nurseries. A.K.M. Jayasena and J.H.N. Piyasundara ANNUAL REPORT FOR 1997 117 4.2.6. VP 67 (VP 39/TJC.l/I) - Evaluation of clones from VP 39 in Field No. 10, StCoombs Estate, Talawakele (1993) Of the 149 clones tested, the yields of the forty-two clones which yielded more than the control clone TRI 2025 are given in Table 11. TABLE 11 - Yield of clones (Kg ha 1 an 1) Clone Yield 245 3892 295 3631 258 3487 265 3473 97 3456 254 3337 238 3302 332 3271 159 3192 267 3165 286 3088 322 3061 268 3045 48 2993 281 2986 273 2964 224 2950 325 2902 178 2887 252 2878 197 2857 271 2840 316 2819 128 2815 261 2809 121 2778 130 2749 292 2743 39 2716 233 2703 288 2679 275 2653 338 2648 278 2648 28 2634 219 2628 263 2608 20 2606 118 ANNUAL REPORT FOR 1997 250 165 318 277 2025 (control) 2601 2587 2569 2558 2528 4.2.7. VP 69 (ASM.INT.88/UC.2/I) - Evaluation of clones developed from ASM 1988 introduction seed in Field No. 10, St.Coombs Estate, Talawakele (1993) The yields of the clones along with the controls are given in Table 12. TABLE 12 - Yield of clones (Kg MT ha-1 an-1) Clone Yield 23 3111 24 3035 Control 1 2723 Control 2 2608 5 2475 34 2449 14 2377 Control 3 2335 SE 395 LSD (P=0.05) 1183 cv% 29.6 4.2.8. VP 70 (VP 39/UC.NOR/l)- Evaluation of clones from VP 39 in Venture Estate, Norwood - (1993) The yields of the seven highest yielding clones out of the 50 tested, along with Oie controls, are given in Table 13. TABLE 13. - Yield of clones (Kg MT ha-1 an-1) Clone Yield 299 3121 295 2805 2025 (control) 2604 278 2547 46 2506 38 2492 303 2407 49 2348 N2 (control) 2365 ANNUAL REPORT FOR 1997 119 4.2.9. VP 71 (VP 39/UC.2/T) - Evaluation of clones from VP 39 in Field No.14, StCoombs Estate, Talawakele (1994) Bushes have been brought into plucking. 4.2.10. VP 72 (VP 43/UC/l) - Evaluation of clones from VP 43 in Field No. 14, StCoombs Estate, Talawakele (1994) Bushes have been brought into plucking. 4.2.11. VP 73 (VP 44/UC/T)- Evaluation of clones from VP 44 in Field No. 14, StCoombs Estate, Talawakele (1994) Bushes have been brought into plucking. 4.2.12. VP 75 (VP 45/UC/T) - Evluation of clones from VP 45 in Field No. 14, St. Coombs Estate, Talawakele (1995) The plants were given a cut at 35 cm (14 inches). 4.2.13. VP 76 (VP 52/UC/T) - Evaluation of clonal selections from polyclonal seed from the seed gardens at Karandupona and Urumiwella (VP 52) in Field No. 14, St. Coombs Estate, Talawakele (1995) The plants were centred in October and infilling was done. 4.2.14. VP 77 (VP 58/UC/I) - Evaluation of clones from polyclonal seed from the seed gardens at Karandupona and Urumiwella (VP 58) in Field No. 12, St. Coombs Estate, Talawakele (1996) The plants were centred in October and infilling carried out. 4.2.15. VP 79 (LVP 49/UC/T) - Evaluation of clones from polyclonal seed from the seed gardens at Karandupona and Urumiwella (LVP 49) in Field No. 12, St. Coombs Estate, Talawakele (1996) The plants were centred in October and infilling carried out. 4.3. Uva 4.3.1. UVP 8 - Evaluation of clones from ASM 1988 Introduction seed from St. Coombs Estate, at TRI Uva station, Passara (1993) 120 ANNUAL REPORT FOR 1997 Clone Yield 2023 (control) 9433 4042 9074 4083 8096 4014 7756 2025 (control) 7088 4075 7056 4080 6862 4046 6834 4076 6669 4015 5703 SE (mean) 1011 LSD (P=0.05) 3235 C V % 21.9 5. Clonal Trials (Phase II) 5.1. Up country 5.1.1. VP 41 and VP 42 - Testing 60 clones at St. Coombs Estate, Talawakele (1984) Shoots harvested from these plots were issued to Estates and Smallholders for establishing '4000' series multiplication plots. 5.1.2. VP 62 - Testing ten 4000 series clones at St. Coombs Estate, Talawakele Plucking was discontinued and the bushes were pruned. 5.1.3. VP 80 (VP 37/UC/n) - Evaluation of clones from VP 37 (St. Coombs) in Larniliere Division, St. Coombs Estate, Estate, Talawakele (1996) The plants were centred in October and infilling was done. V. Shanmugarajah, M. Ratnayake, S.W. Gunadasa, B.A. Rathnagoda and S. Umah 5.2. Low country 5.2.1. LVP 43 - Testing 42 clones on Diyadawa Estate, Deniyaya (1990) 5.2.2. LVP 60 - Testing eight clones at Golinda estate, Kegalle Yields of the clones along with the controls are given in Table 14. TABLE 14 - Yield of clones (Kg MT ha-1 an-1) ANNUAL REPORT FOR 1997 121 5.2.3. LVP 74 (LVP 30/LC/TI) - Evaluation of clones from LVP 55 in Handford Estate, Deniyaya at TRI Deniyaya station (1997) Promising clones from LVP 55 trial were propagated and planted in August. Number of clones - 35 Number of plants - 24 (per replicate) Number of replicates - 02 Control clone - TRI 2026 M. Ratnayake, S.W. Gunadasa, V. Shanmugarajah, B.A. Rathnagoda, S.L.D. Amarathunga and J.A.S.K.V. Jayasinghe 5.2.4. LVP 75 (LVP 56, 57, 58, 59/LC/H) - Evaluation of clones from LVP 56, 57, 58 and 59 of Field No.5, St. Joachim Estate in Field No. 1 of St Joachim Estate, Ratnapura (1997) Promising clones from LVP 56, 57, 58 and 59 were propagated and planted in the field in July for further evaluation. Number of clones - 40 Number of plants - 25 (per replicate) Number of replicates - 2 Control clones - TRI 2023,2026,2027 A.K.M. Jayasena, J.H.N. Piyasundara, M. Ratnayake, S.W. Gunadasa and V. Shanmugarajah 5.3. Uva 5.3.1. UVP 7 - Testing 38 clones at TRI Uva station, Passara (Pelagahatenne) Shoots harvested from these plots were issued to Estates and smallholders for establishing '4000' series multiplication plots. M.B.A. Perera and V. Shanmugarajah 6. Evaluation of '3000' and '4000' series clones (Phase III trial) 6.1. Up country 6.1.1. VP 50 - St. Coombs Estate, Lamiliere Division (1990) Shoots harvested from these plots were issued to estates and small holders. S.W. Gunadasa and V. Shanmugarajah t j. 122 ANNUAL REPORT FOR 1997 Clone Yield 3020 5711 3048 5360 3016 5184 3069 4607 2025 (control) 4414 DN (control) 3980 3019 3826 3017 3211 3015 3098 3013 3079 3018 3024 K145 (control) 3010 3014 2907 SE (1 mean) 1195 LSD (P=0.05) 3683 C V % 34.9 6.13. Gordon Estate, Udapussellawa - (1991) The yields of the clones along with controls are given in Table 16. TABLE 16 - Yield of clones (Kg MT ha"1 an 1) Clone Yield 3015 4061 2025 (control) 3760 PK2 (control) 3628 3017 2865 DT 1 (control) 2694 3018 2613 3016 2448 6.1.4. VP 59 - St. Coombs Estate, Field No. 10 - (1992) Bushes were pruned to be used as mother bushes. 6.1.5. Venture Estate, Norwood. Field No. 6A - (1993) Yields of the clones along with the control are given in Table 17. 6.1.2. Stockholm Estate, Nissanka Uyana (1991) The yields of the clones along with control clones are given in Table 15. TABLE 15 - Yield of clones (Kg MT ha1 an 1) ANNUAL REPORT FOR 1997 123 TABLE 17 - Yield of clones (Kg MT ha 1 an1) Clone Yield 4071 3662 2025 (control) 3285 3069 3197 3016 3139 3073 2981 4052 2551 3019 2509 4063 2459 3072 2266 4053 2032 3020 1775 3015 1753 3018 1312 3017 719 6.1.7. Sheen Group, Pundaluoya. Field No.3 NC 5A - (1992) The yields of the clones along with the control are given in Table 18. TABLE 18 - Yield of clones (Kg MT ha"1 an 1) Clone 4078 4053 4083 4075 2025 (control) 3069 4079 4076 4071 4053 Yield 6223 5676 5538 4940 4730 4606 4016 3477 3247 2682 S.W. Gunadasa, M. Ratnayake, B.A. Rathnagoda, S. Umah and V. Shanmugarajah 6.2. Uva 6.2.1. Verellapatana Estate - Doomo Division -1988 The bushes are being plucked by the estate. 6.2.2. Small Holdings in Bandarawela, Haliela and Boralanda (1992) Plucking was discontinued, the bushes were pruned and weight of prunings recorded. 124 ANNUAL REPORT FOR 1997 6.23. TSHDA - Hali-Ela (1992) Bushes were brought into plucking and plucking was continued by the TSHDA. M. Ratnayake, S.W. Gunadasa and B.A. Rathnaagoda 63. Mid country wet zone 63.1. Hantane Estate - (1991) 63.2. Small-holdings in Ukuwela, Danture, Teldeniya, Dodanwela and TSHDA nursery Muruthalawa. The yield of the clones at the TSHDA, Muruthalawa are given in Table 19. TABLE 19 - Yield of clones (Kg MT ha 1 an 1) Clone Yield 4046 7366 3018 6343 4006 6468 2025 (control) 5731 3015 5615 4042 5276 3013 5147 3020 4952 3014 4742 4053 4404 3019 4159 4047 3029 SE 672 LSD (P=0.05) 1890 cv% 25.5 V. Shanmugarajah, M. Ratnayake, S.W.Gunadasa, S. Umah, B.A. Rathnagoda and H.M.S.S.K. Herath ANNUAL REPORT FOR 1997 125 6.4. Low country 6.4.1. LVP 38 - Watapotha Estate (1988) The yields of the clones along with controls are given in Table 20. TABLE 20 - Yield of clones (Kg MT ha' an1) Clone Yield 4056 5315 3020 4425 4059 5278 4054 5974 DG 39 (control) 6602 4055 6958 4047 6453 KEN 16/3 (control) 5935 SE (mean) 418 LSD (P=0.05) 1338 CV % 17.5 The bushes were pruned, weight of prunings recorded and the plots were handed over to the estate. V Shanmugarajah, A.K.M. Jayasena, J.H.N. Piyasundara, M. Ratnayake, S.W. Gunadasa, B.A. Rathnagoda and S. Umah 6.4.2. Smallholding at Dehiowita Yields of the eleven clones along with the controls are given in Table 21. TABLE 21 - Yield of clones (Kg MT ha"1 an 1) Clone Yield 4006 7864 4053 7530 2023 (control) 7493 4061 7234 4033 6857 2027 (control) 6845 4055 6720 2025 (control) 6581 4059 6218 4004 6082 4049 5960 4054 5676 4048 4319 4024 3748 126 ANNUAL REPORT FOR 1997 6.4.3. LVP 65 - St Joachim Estate, Ratnapura - Multiplication rows of 4000 series and estate clones. Cuttings from these plots were issued to estates and smallholders. A.K.M. Jayasena, J.H.N. Piyasundara and V. Shanmugarajah 7. Supply of clonal cuttings Cuttings of 3000 and 4000 series clones were supplied to estates and small holdings in the up-country, Uva, mid-country wet zone, and low-country for the purpose of estabhshing mother bushes and evaluation trials. The estates to which the cuttings were issued, the clones, and approximate numbers of cuttings issued are given below. 7.1. Up country Estate Clones Shoots Attampitiya 3013, 3014, 3016, Ettampitiya 3019, 3020, 3035, 4043,4044,4052 50 each 4006,4070, 4079 100 each 4047 125 4042,4046,4053, 150 each 4071 200 TSHDA 3020, 3047, 300 each Bandarawela 4006 200 3049, 3052 150 each 4071 800 -j 3072, 3073,4052 4053,4079 500 each Frotoft 3046, 3048,4006 75 each Ramboda 3020, 3047, 3049, 3072, 3073,4067, 4071,4078,4079 100 each Liddesdale 3031,3039, 3041, Nanuoya 4045 50 each 3014, 3025, 4042, 4046,4070 150 each 4040,4047 100 each 4006 200 ANNUAL REPORT FOR 1997 Radella 3013, 3014, 3015, Nanuoya 3016, 3017, 3018, 3019, 3048, 3052, 3069, 3072, 3073 50 each 3020 100 4052,4053 200 each Mountjean 3013, 3014, 3020, Watawala 3069,3072,3073 50 each 4006,4052,4053, 4063, 4071,4078 75 each Rappahannock 3015, 3016, 3017, Udapussellawa 3018, 3019, 3020, 3025, 3069,4042, 4043,4046,4070 50 each 3072, 3073 100 each 4063 150 3013, 3047, 3069, 3073, 4052,4067, 4079 200 each 3014,4053, 4071, 300 each Hope 4006,4042 50 each Hewaheta 4046,4047, 4052, 4070 100 each 4071,4078 150 each 4053 200 Mooloya 4024 50 Hewaheta 3049,3072,3073 100 each 4006,4063,4067 200 each 3046 250 3013,3069,4053 300 each 4052,4071 550 each 3014, 400 4078, 4079 650 each Robgill 3014,3019,3020, Bogawantalawa 3046, 3047, 3048, 4052, 4071,4078 50 each 4079 25 3072, 3073 350 each 128 ANNUAL REPORT FOR 1997 Binoya 'B' 3013, 3014, 3015, Rozella 3016, 3017, 3018, Watawala 3019, 3020, 4006, 4083 50 each 3069, 3072, 3073, 4052,4078,4079 175 each 3047,3049 75 each 4053,4071 125 each 4063 225 Norwood 3048 400 Norwood 3046,3069, 200 each 3047, 3072, 3073, 4006,4063 300 each 4052,4053, 500 each 4079 800 Rockwood 3046,3048,3069 100 each Norwood 3047,4006 200 each 4079 500 New valley 3049,4079 200 each 3052, 4052 300 each Kelliewatta 4006, 4052, 4053, Patana 4067,4071 100 each 4083,4085 25 each 4063 50 4078,4079 200 each Delta 3025 50 Pupuressa 4006,4043,4046, 4047, 4052, 4053, 4071,4078 250 each 3013, 3014, 3016, 3017, 3018, 3019, 3020, 3072, 3073 300 each Troup 4006,4052,4053, Talawakele 4063,4067,4071, 4078, 4079, 4083, 4085 100 each Helbodde 3013, 3014, 3015, Katukitula 3016, 3018, 3019, 3020 200 each ANNUAL REPORT FOR 1997 4006, 4042, 4046, 4047,4053,4071, 250 each Downside 3013,3016,3017, Welimada 3018,3019,3031, 3039,4042,4043 50 each 4046,4052,4053, 4079 100 each 4071.4078 225 each TSHDA 3020,4071 300 each Gampola 4006 200 3049 150 3052 100 Ireby 3072,3073 500 each Norwood Diyanilakelle 4052 150 Lindula 3072, 3073,4053, 4071,4078,4079 100 each 3016, 3017, 3019, 4083,4085 50 each MorawakkaElla 4052 200 4049,4054 50 each Prof. Sam Casie 3014, 3016, 3017, Chetty 3025, 3049, 3069, Hatton 4006, 4042,4046, 4052,4053,4071, 4078.4079 50 each 7.2. Low country Estates Aigburth 3014, 3041, 3052, Bulutota 3055, 3060,4014, 4046, 4047,4053, 4055,4059,4061 50 each Kelani 3041, 3046, 3047, Yatiyantota 3055, 3058,4014, 4042, 4046,4047, 4053,4055,4061 50 each 130 ANNUAL REPORT FOR 1997 Geekiyanakanda Neboda Pelawatta Pelawatta Yataderiya Undugoda Keeragala Kuruwita Mohamadi Agalawatta Vogan Mattugama Pambegama Godagampola Herningford Parakaduwa Katandola Elpitiya Hopton Sapumalkanda Deraniyagala 3014, 3055 3041, 3046, 3047, 3055, 3058,4002, 4014,4047,4059, 4061 4004,4043,4052, 4053,4054,4055, 4061 4042,4044,4046 2027 3046, 3055,4047, 4053,4059 2025, 3014, 3041, 4049,4061 2027 ' 3055, 3060,4047, 4049,4055,4059 4048,4061,4062 4014,4042,4044, 4046,4047,4048, 4056,4059,4061 4062 3014, 3025, 3041, 3046, 3055,4047, 4049,4059,4061, 4062 3041,4044,4046, 4047,4053,4059, 4061 2027 3025 3047, 3055,4059, 4061 3025,4042,4043 200 25 50 each 50 each 75 each 500 50 each 100 each 200 50 each 100 each 50 each 50 each 50 each 600 100 50 each 100 each ANNUAL REPORT FOR 1997 Smallholders TSHDA Kegalle Samitha Attigala Hewawitharana Pelmadulla K.Suriyarachchi Nugegoda, Ratnapura TSHDA, Matugama LJ.Liyanage Batuhena W.Somasiru Pelmadulla Greenrock Dehiowita D.Perera Matugama K.B.Prerriathilaka Kuruwita 3041, 3046, 3055, 4061 200 each 3014,4055 300 each 3047,4049 50 each 2025 1000 2027, 3046 100 each 3014,4061 100 each 3025, 3052, 3058, 4002,4042,4046 50 each 3014, 3046 100 each 3055 400 2025, 2027 200 each 3047, 3055, 3060 50 each 3014 100 2025 500 3041, 3055,4061 100 each 3025, 3046, 3047 4047,4049,4053 50 each 2025 200 3046 50 3046, 3047, 3055 4061 50 each 3014, 3025, 3041 100 each 2027,4059,4061 50 each 2027 200 3014, 3025 100 each 2025,2027 100 each Chaminda Kumaranayake Hidellana 2025 500 132 ANNUAL REPORT FOR 1997 Rajapaksha Ratnapura 2027 4042,4049 500 50 each Mahinda Liyanage 2027 2025 400 100 7.3. Mid - country 7.4. Uva 8. Seed Gardens Seed gardens in St Coombs and at Hantane station were used for controlled hybridisation. 9. Divisional Activities Observation trial An observation trial was carried out to see the effect of different sizes of bags on the growth of tiie plants in the nursery. Along with standard size (9" x 5" lay flat) bags of 4 1/2" x 5" Gay flat) were used. Better plant growth with respect to plant height, number of leaves produced and dry weight was observed with plants grown in smaller bags. Use of smaller bags helped save on soil and labour. Germplasm Bank Action was initiated to establish germplasm banks at the TRI stations at Ratnapura, Hantane, Passara and Talgampola. Bushes in the germplasm bsxtk at the Head Office were pruned to supply cuttings to the above stations. At the low country station Ratnapura a germplasm bank was commenced by planting 50 clones (of the 2000, 3000 and 4000 series) initially. Canker Assessment With the help of Pathology Division staff canker assessment was carried out in all Phase I trials at the low country station, Ratnapura. Quality Assessment Flush was collected from some of the 3000 and 4000 series clones and supplied to the Biochemistry and Technology Divisions for assessment of quality of the made tea. The following are the estates and the clones used for the above assessment: ANNUAL REPORT FOR 1997 133 Sheen Group, Pundaluoya - TRI 4052,4071, 4078,4079 Venture Estate, Norwood - TRI 3016, 3018, 3072, 3073, 4052, 4053,4063 Stockholm Estate, Upcot - TRI 3015, 3016, 3018, 3019, 3020, 3048 Large scale planting At Norwood estate 3900 and 2800 plants of clones TRI 3073 and 4006 respectively were planted in August for evaluation. 9. Project B/TC Tissue Culture Project Leader S.K. Sathyapala 9.1. Development of homozygous plants through anther and pollen culture of tea. A project was initiated in collaboration with the IFS to investigate the crucial factors for production of haploid plants from anther and pollen cultures in tea and other Camellia species. Under this project the following aspects are being investigated: 9.1.1. Effect of pre-treatments of anthers such as pre-treatment with colchicine lmg-lOmg, sugar starvation by dipping anthers in 0.3M - 0.1M manitol solution, and exposure of anthers to 32-35EC heat for 3-5 days. 9.1.2. Studies on the effect of different culture media (MS, B5 and N6) and plant growth regulators (2,4-D, IAA, Zeatin and BAP) on embryogenesis and callus induction in pollens. 9.1.3. Effect of the genotype variations on induction of embryogenesis in anther cultures. Anthers of clones TRI 2025 and DG 7 and that of C. japonica are being used. These experiments are in progress. S.K. Sathyapala, H. Jayaweera, K. Sarathchandra, and M. Iqbal 9.2. Induction of callus in different organs of tea, C. sasanqua and C. japonica and development of cell suspension systems for in vitro selection, isolation of protoplasts and somatic hybridization. It is well known that cell suspensions are ideal for isolation of protoplasts, in vitro mutant selection and genetic transformations. Further embryogenic 134 ANNUAL REPORT FOR 1997 callus and cell suspensions could be used for selection of stress-tolerant cell lines and subsequent production of drought and salt tolerant tea plants. To initiate embryogenic cell suspension the following experiments were commenced: 9.2.1. Effect of the origin of callus on growth of the cell suspensions. Treatments: 1. Stem callus 2. Leaf callus 3. Cotyledon callus 4. Hypocotyl callus From each callus 0.5g was cultured in the liquid medium supplemented with 2,4-D and Kinetin. Every 10 days the cell suspensions are being subcultured and the following records were collected: Packed cell volume Mitotic index A similar experiment is being carried out with the C. sasanqua and C. japonica explants. 9.2.2. Effect of different growth regulators on growth of the suspension cultures of TRI 2025 and C.sasanqua Treatments: 1. 2,4-D lmg/1 and 10% coconut water 2. 2,4-D 2mg/l and 10% coconut water 3. 2,4-D lmg/1 and 0.5 kinetin 4. 2,4-D 2mg/l and lmg/1 kinetin 5. 2,4-D lmg/1 and 0.5 mg/1 IAA 6. Kinetin lmg/1 and 0.5 mg/1 IAA The cell growth was measured by - 1. Packed cell volume 2. Mitotic index These experiments are in progress. S.K. Sathyapala, T.M. Sarathchandra, P.D. Upali and H. Jayaweera 93. Identification of contaminants in in vitro cultures of tea. A systematic study on the bacterial contaminants of tea tissue cultures may facilitate identification of the major systemic contaminants and their subsequent elimination. With this in mind, the following experiment has been initiated: ANNUAL REPORT FOR 1997 135 9.3.1. Isolation and identification of major contaminants present in the initial stage of the different in vitro cultures of tea. The rate of bacterial contamination in different explants of tea grown under in vitro conditions is given in Table 1. TABLE 1 - Percent bacterial contamination Treatment No. of percent explants B.contamination Anthers 152 9.21 Nodal explants 205 60.48 Cotyledon 75 12.00 Embryo 75 4.00 Among four different in vitro cultures- anther, nodal explant, cotyledon callus and embryo, the highest rate of contamination was found in nodal explants. The other three cultures had significantly low rate of contamination. The isolation and identification of the contaminants and antibiotic sensitivity tests are in progress. S.K. Sathyapala and T.M. Sarathchandra 9.4. Improvement of direct and indirect embryogenesis in different explants of tea and C. sasanqua Somatic embryogenesis is considered to be the most efficient means for clonal multiplication, somaclonal variation, artificial seed production and cryopreservation for the establishment of gene banks. A study was commenced to improve the rate of somatic embryo formation in different organs of tea and their development in to plantlets. 9.4.1. Somatic embryogenesis in cotyledon and hypocotyl explants of tea and C. sasanqua The somatic embryos developed from cotyledon and hypocotyl explants of tea and C. sasanqua in MS medium supplemented with BAP and IAA have been transferred to the hormone-free MS half strength medium for further growth. Morphological characteristics of the somatic embryos in the germination medium are being investigated. 9.4.2. Somatic embryogenesis in leaf explants of tea Leaf callus has been cultured in solid and in agitated liquid medium to induce the embryogenesis. The callus fragments in the liquid medium showed 136 ANNUAL REPORT FOR 1997 high potential for embryogenesis. The effect of plant growth regulators on direct embryogenesis is being investigated. Treatments: 1. BAP lmg/1 2. BAP lmg/1, IBA 0.1 mg/1 and Zeatin 0.2mg/l 3. BAP 3mg/l, IBA 0. lmg/1 and Zeatin 0.4 mg/1 4. Zeatin lmg/1 and IBA0.1 mg/1 Observations on embryo formation and organogenesis are in progress. 9.4.3. Somatic embryo formation in stem explants Pre-embryo formation was found in stem callus of clones TRI 2025 and TRI 4006 cultured in MS solid medium supplemented with BAP and NAA. However, most of the stem callus has shown a tendency to organogenesis. Further investigations to improve the embryo formation using different growth regulators are in progress. S.K. Sathyapala, P.D. Upali and T.M. Sarathchandra. 9.5. A study on die-back of TRI 2025 at High forest Estate This study was initiated in collaboration with IPS to ascertain the cause for die-back of TRI 2025 plants in High forest Estate. 9.5.1. About 300 plantlets of TRI 2025 were grown under in vitro conditions to be used in bioassay experiments on xylem sap of the affected plants. Treatments: 1. Control - Inoculation of distilled water to in vitro grown TRI 2025 2. Inoculation of xylem sap of the affected plants to in vitro plantlets 3. Inoculation of xylem sap of healthy TRI 2025 to in vitro plantlets This experiment is in progress. N. Iddagoda, P.D. Upali and S.K. Sathyapala 9.6. Implementation of embryo culture and micro propagation in the conventional breeding program. The selection and production of new clones by conventional breeding method takes about 15-20 years. From seed to mother bushes in the observation plots takes 6-7 years, but this may be reduced to 3-5 years by using methods of embryo culture and micro propagation. However, absence of selection criteria for in vitro cultures has hindered the application of this technique. ANNUAL REPORT FOR 1997 137 9.6.1. Development of selection criteria for in vitro growing plants A new study has been initiated to investigate the growth and performance of in vitro grown seedlings and their micropropagated plants to detennine the selection criteria potential for use in the breeding program. Seeds of Poonagala estate seed bearers were planted in vitro and in sand beds to obtain seedlings. The following growth assessments were monitored for embryo cultures: Length of the shoot Length of roots Plant height Number of leaves per plant Number and length of internodes Plant vigour Except for the length of the roots, the other growth assessments will be monitored for the seedlings raised in sand beds. 9.6.2. Application of embryo culture to wide hybridization of tea and other Camellia species for selection. To obtain seeds for embryo cultures, controlled hybridization was carried out between china jat plants and TRI 2025. Embryos of seeds from crosses of C. sasanqua and C. sinensis were cultured in vitro and morphological attributes of these embryos are being investigated. These experiments are in progress. S.K. Sathyapala and P.D. Upali 9.7. Studies on the field performance of tissue cultured plants. 9.7.1. Micropropagation of clones for field evaluation Clones TRI 2025,4006,4053 and DG7, are being micropropagated for comparison of their performance with the vegetatively propagated plants of the same clones in the field. This study is in progress. T.M. Sarathchandra, P.D. Upali, H. Jayaweera and K. Sarathchandra 138 ANNUAL REPORT FOR 1997 9.7.2. Somaclonal variation among the field planted tissue cultured tea plants at Hantana and StCoombs Estate Periodic growth assessments of in vitro grown clone TRI 3031 are being carried out to determine somaclonal variation among tissue cultured plants. The growth parameters such as yield, shoot growth, fresh and dry weight are being monitored. This experiment is in progress. S.K. Sathyapala and P.D. Upali 9.73. Micropropagation of other trees found in tea plantations After acclimatization in the green house, 15 in vitro grown Eucalyptus plants were planted in the field at Hantana substation. This experiment was terminated with effect from November 20 after perfecting a method for micro propagation of Eucalyptus. The original Eucalyptus plants were a gift from Australia in 1993. S. K. Sathyapala and P.D. Upali 11. Visitors Dr P. Huxley, Director, ICRAF, Nairobi visited the lab, seed bearer area, original bushes of the 2000 series clones and the nursery 28th May. Capt. Migara de Zoysa, Direcor, Chilaw Plantations Ltd. visited the Division and had discussions on various aspects of development of clones and on the clones suited for different regions on 4th June. Prof Dennis A. Baker, Head, Dept. of Biological Sciences, Wye College, University of London visited the lab November 24. Students from Peradeniya,Eastern and Sabragamuwa Universities visited the Division and the nursery. Six Assistant Superintendents from various estates were in this division on a familiarisation programme. 12. Seminars/ Training Programmes/ Meetings Mr V. Shanmugarajah was a member of the team that participated in discussions with the following: CEO's of the Plantation Management Companies held at the CFC auditorium, Colombo (March 8th). ANNUAL REPORT FOR 1997 139 Members of the RSC I, Nuwra Eliya on "Clones for Nuwara Eliya" (May 5th). TSHDA Officials (July 7th). Executives of Agrapatna and Kotagala Plantations (August 8th) Dr. S.K. Sathyapala attended a seminar on "Application of Molecular biology in Sri Lanka" organized by SLAAS at SLAAS Auditorium on September 15th. Dr. S. K. Sathyapala attended a seminar on "Genetic conservation of traditional plants" organized by ARTI, at the National library on December 5th. Mr S.W. Gunadasa was a member of the team that had discussions with the members of the RSC I, Nuwara Eliya on "Clones for Nuwara Eliya" (May 5th). Messrs T.M. Sarathchandra and P.D. Upali participated in Plant Biotechnology Working Group meetings held at the Institute of Fundamental Studies (March 27th) and at the Rubber Research Institute (June 6th). Attendance at International Conference Along with the Director, Mr V. Shanmugarajah attended the Second Asia International Tea Conference held at the Hilton International, Singapore from September 24th to 26th and presented a paper on "Clonal developments in Sri Lankan tea and their potential for the trade". 14. Publications Modder, W.W.D, Shanmugarajah, V. and Sivaram, B. "Clonal developments in Sri Lankan tea and their potential for the trade." Proceedings of the Second Asia International Tea conference, September 24-26. Sathyapala, S.K. and Adachi, T. "Plant Regeneration from embryogenic cell suspensions of tea" SABRAO Journal 28(2) 57-65. 140 ANNUAL REPORT FOR 1997 Acknowledgement We are grateful to the following for their cooperation in carrying out our trials in their fields: Plantation Management Companies Superintendents and Assistant Superintendents of Golinda Estate, Kegalle Gordon Estate, Udapussellawa Sheen Estate, Pundaluoya Stockholm Estate, Nissanka Uyana (Upcot) Venture Estate, Norwood Watapotha Estate, Watapotha Mr A.Somaratne, Deputy General Manager, TSHDA and Mr H.M.S.S.K.Herath of the TSHDA Muruthalawa Smallholders in different areas. SOILS AND PLANT NUTRITION DIVISION Acting Head - L.S.K.Hettiarachchi 1. General Mr A.K.N.Zoysa, Research Assistant continued his post graduate studies at Massey University, New Zealand. Mr H.A.P.Warnasiri, Technical Assistant, was awarded the B.Sc degree by the University of Peradeniya with effect from 26th October, 1996. Mr W.M.S.Wijethunge, Experimental Officer, commenced his Masters degree in Analytical Chemistry at the Post Graduate Institute of Science, Peradeniya with effect from 04th July 1997. Dr L.S.K.Hettiarachchi continued to serve as a member of the Working Group on Fertilizer to the Sri Lanka Standards Institution. He served as a member of the Executive Committee of the Soil Science Society of Sri Lanka. The overall research objective of this division is to improve soil fertility of tea lands in Sri Lanka by efficient use and/or control of both organic and inorganic fertilizers to maximize the productivity. Field experiments have been carried out and reported under B/FERT whereas the laboratory and glasshouse experiments carried out have been under D/AGCH. Developments in relation to soil and plant chemical aspects, and soil, plant and feitjlizer analyses carried out have been reported under C/ANAL. 2. Project B/FERT - Improvement and maintenance of fertility and productivity of tea soils. Project Leader - L.S.K.Hettiarachchi 2.1. Soil and Fertilizer Nitrogen Studies 2.1.1. Application of different proportions of sulphate of ammonia and urea on soil/plant sulphur status and yield of tea, St.Coombs Estate, Talawakele - Clone TRI 2025 (1980). The yield obtained in the 1st year of the 5th cycle is given in Table 1. In the 1st year, no significant differences in yield were found between 240 and 360 kg of N ha-1 yr 1 applications. However, application of N at 360 kg ha"1 yr 1 yielded more than the 240 kg ha 1 yr 1. Also, no significant differences in yield were observed between any of the treatment combinations. 142 A N N U A L R E P O R T F O R 1997 TABLE 1 - Effect of different proportions ofSA/urea on yield (made tea kg ha'1 yr') of mature tea Levels ofN Proportions (%) of Yield (kg ha' yr') SA (N) Urea (N) (made tea kg ha' yr') 240 360 100 0 1047 75 25 1090 50 50 1105 25 75 1098 0 100 1078 100 0 1255 75 25 1159 50 50 1178 25 75 1133 0 100 1113 .05) NS 10 cv% The effect of application of SA/urea on soil pH and sulphate sulphur levels (0-15 & 15-30 cm) are given in Table 2 and 3 respectively. TABLE 2 - Effect of application of different proportions of SA/urea on soil pH levels at 0-15 and 15-30 cm depths Proportionsf %)of Soil pH SA(N) Urea (N) 0-15 cm 15-30 cm 100 0 4.60 4.30 75 25 4.28 4.30 50 50 4.37 4.40 25 75 4.60 4.63 0 100 4.70 4.72 LSD (P = 0.05) 0.298 0.305 cv% 5 5 Sub Level ofN (kg ha' yr') 240 4.54 4.61 360 4.48 4.33 LSD (P = 0.05) NS 0.193 In general, soil pH levels at both depths decreased with increased proportions of SA in SA/urea combinations, except at 0-15cm depth with 100:0 SA:urea combination, even after application of dolomitic limestone at 1000 kg ha1 after pruning. However, no significant differences in pH were found between 25:75 and 0:100 SA:urea applications. Although, no significant difference in soil pH levels between 240 and 360 kg of N ha-1 yr 1 was observed at 0-15cm ANNUAL REPORT FOR 1997 143 depth, at 15-30cm depth significant difference exist. This is probably due to dolomitic limestone application. TABLE 3 - Effect of application of different proportions of SA/urea on soil sulphate sulphur levels at 0-15 and 15-30 cm depths Proportions(%) of SOf-S (mgkg1) SA (N) Urea (N) 0-15 cm 15-30 cm 100 0 133 179 75 25 137 163 50 50 143 164 25 75 112 128 0 100 80 93 LSD (P = 0.05) 51.0 47.6 cv% 32 25 Sub Level ofN (kg ha1 yr1) 240 123 159 360 119 132 LSD (P = 0.05) NS NS No significant differences in soil sulphate sulphur levels were found between two N levels, at both depths. However, significant increases in sulphate sulphur levels were found between 100:0,75:25 and 50:50 SA:urea applications and 0:100 SA:urea application, at both depths. S.Ananthacumaraswamy and L.S.K.Hettiarachchi 2.1.2. Application of very high levels of urea and sulphate of ammonia on soil/ plant nutrient status and yield of tea, StCoombs Estate, Talawakele - Clone TRI 2025 (1980). This is an observation trial established in 1990 to study the ill effects of high levels of nitrogen. The yield obtained for the period July 1996 to June 1997 i.e. 1st year of the 2nd cycle is given in Table 4. TABLE 4 - Effect of application of very high levels of SA and urea on yield of tea Nitrogen Yield (kg ha'1 yr') (made tea kg ha'1 yr1) Urea SA 0 772 773 300 819 775 600 766 812 900 1001 923 1200 906 894 1500 860 1052 144 ANNUAL REPORT FOR 1997 During the 1st year after pruning, in general, consistent trends in yield were not observed with the increasing rates of N and two different forms. This experiment is in progress. G.P.Gunaratne and L.S.K.Hettiarachchi 2.1.3. Improvement of soil organic matter status and efficiency of uptake of inorganic fertilizer nutrients by incorporation of different sources of organic-manure, Bearwell Estate, Talawakele - Clone TRI 2025 (1990). The yield obtained in the 2nd year of the 2nd cycle and soil pH levels at 0-15cm depth are presented in Table 5 and 6 respectively. TABLE 5 - Effect of levels and sources of organic manure at 0 and 240 kg N ha'1 yr' on the yield (made tea kg ha'1 yr') of tea Level of organic manure CMtha'yr') 5 10 Level ofN(kg ha' yr') 0 240 0 240 Compost 2281 2622 2235 2463 Cow dung 2344 2340 2277 2496 Mana 2342 2570 2269 2459 Gautemala 2320 2248 2316 2478 LSD(P=0.05) 281 CV % 7 Type of Organic manure Compost 2400 LSD(P=0.05) Level of organic manure CMtha'yr') 5 2383 LSD(P=0.05) Level of N(kgha'yr') 0 Cow dung Mana Gautemala 2364 2410 2341 NS 10 2374 NS 240 2460 99.5 LSD(P=0.05) * Mt-Metric tons 2298 ANNUAL REPORT FOR 1997 145 TABLE 6 - Effect of levels and sources of organic manure at 0 and 240 kg N ha'1 yr' on the soil pH levels at 0-15 cm depth, Level of organic manure CMtha'yr') 5 10 Level ofN (kg ha' yr') 0 240 0 240 Compost 4.69 4.52 4.48 4.41 Cow dung 4.53 4.30 4.64 4.12 Mana 4.60 4.26 4.49 4.24 Gautemala 4.66 4.18 4.74 4.33 LSD(P=0.05) 0.359 C V % 5 Type of Organic manure Compost Cow dung Mana Gaut 4.52 4.40 4.40 4.48 LSD(P=0.05) NS Level of organic manure CMtha'yr') 5 10 4.47 4.43 LSD(P=0.05) NS Level of N(kgha'yr') 0 240 4.60 4.29 LSD(P=0.05) 0.127 * Mt-Metric tons Significant differences in yield were not found either between source and level of organic manure both at 0 and 240 kg ofN ha-1 yr 1 levels, or due to sources and levels of organic manure alone. However, yields were significantly increased due to application of N at 240 kg ha"1 yr 1 when compared to zero. Also, as far as soil pH levels are concerned, no significant effects were found due to sources and levels of organic manure. However, pH levels dropped significantly due to application of N at 240 kg ha"1 yr 1. This experiment is in progress. S.M.Dissanayake, A.K.N.Zoysa and L.S.K.Hettiarachchi 2.1.4. Effect of different levels of nitrogen (0-720 kg ha"1 yr 1 N at 120 kg N increment) with and without (0 and 51 ha"1 yr 1) compost manure on soil/ plant - N status and yield of tea, StCoombs Estate, Talawakele - Clone DTI (1992). The yield obtained in the 5th year of the 1st cycle is given in Table 7. 146 ANNUAL REPORT FOR 1997 TABLE 7 - Effect of different levels of nitrogen with and without compost on yield Treatments Yield (made tea kg ha1 yr1) Nlevel 0 1909 120 2391 240 2335 360 2395 480 2537 600 2350 720 2385 LSD (P=0.05) NS cv% 6 Sub No compost 2372 5 t ha 1 yr 1 compost 2289 LSD (P=0.05) NS Significant differences in yield were not observed due to incorporation of compost. Although no consistent significant increases in yield were observed with the increasing rates of N, trend exist up to 480 kg ha-1 yr 1 N, and thereafter drop. This experiment is in progress. R.G.A.Wijayawardhana and L.S.K.Hettiarachchi 2.2. Soil and Fertilizer Potassium Studies 2.2.1. Split application of nitrogen and potassium fertilizer in mature tea in relation to N/K antagonism, StCoombs Estate, Talawakele - Clone TRI 2025 (1990). The yield obtained in the 1st year of the 2nd cycle i.e. July 1996 to June 1997 is presented in Table 8. ANNUAL REPORT FOR 1997 147 TABLE 8 - Effect of split application of potassium on yieldfmade tea kg ha'' yr') of tea Field Kfertilizer applied (%) K- Fertilizer (kg K20 ha'1 yr') Initially 6 weeks after 120 240 100 0 864 839 80 20 905 913 60 40 849 925 40 60 875 840 20 80 936 824 0 100 874 838 LSD (P=0.05) 78 C V % 6 Significant differences in yield were not observed either due to split applications or due to different K fertilizer rates, although differences between some mean yields were greater than the LSD value. This experiment is in progress. G.P.Gunaratne and L.S.K.Hettiarachchi 2.2.2. Effect of application of increasing levels of potash (60-360 kg ha 1 yr 1) at 60 increments with N (240 and 360 kg ha 1 yr 1) on soil/plant K and Mg status and yield of tea, Halgolla Estate, Yatiyantota - Clone TRI 2025 (1984). After the completion of 3rd pruning cycle and assessments of data, investigations have been concluded. A part of data was presented to the 195th E & E forum, and the overall results will be published as scientific findings from a paper. G.P.Gunaratne and L.S.K.Hettiarachchi 2.2.3. The effect of application of potash (48 to 480 kg ha"1 yr 1 IC,0) with and without Mg (0 and 60 kg ha1 yr 1 MgO) fertilizer, on soil/plant K/Mg status and yield of tea, Glenanore Estate, Haputale - TRI 2025 (1991). The yield obtained in the 2nd year of the 2nd cycle is given in Table 9. 148 ANNUAL REPORT FOR 1997 TABLE 9 - Effect of different N:K20 ratios and MgO on yield of tea Level (kg ha' yr') N K20 MgO N:K20 Yield (made tea kg ha' yr') 240 48 0 5:1 2672 240 48 60 5:1 2609 240 60 0 4:1 2718 240 60 60 4:1 2655 240 80 0 3:1 2711 240 80 60 3:1 2659 240 120 0 2:1 2636 240 120 60 2:1 2655 240 240 0 1:1 2735 240 240 60 1:1 2628 240 360 0 2:3 2675 240 360 60 2:3 2703 240 480 0 1:2 2626 240 480 60 1:2 2701 LSD (P=0.05) NS CV % 4 Sub treatments MgO (kg ha' yr') 0 2682 60 2659 LSD(P=0.05) NS Significant differences in yield were not observed due to different N ^ O ratios, or increased potash rates or due to application of MgO at 60 kg ha-1 yr 1. The effect of NIKJO ratios and of MgO on soil K and Mg status are given in Tables 10 and 11 respectively. ANNUAL REPORT FOR 1997 149 TABLE 10 - Effect of different N:K20 ratios and MgO on soil K status at two soil depths Level (kg ha'yr1) Soil K (mg kg1) N K20 MgO N:K20 0-15 cm 15-30 cm 240 48 0 5:1 130 122 240 48 60 5:1 120 110 240 60 0 4:1 150 154 240 60 60 4:1 125 117 240 80 0 3:1 157 142 240 80 60 3:1 222 162 240 120 0 2:1 207 175 240 120 60 2:1 140 132 240 240 0 1:1 212 195 240 240 60 1:1 162 150 240 360 0 2:3 220 230 240 360 60 2:3 207 190 240 480 0 1:2 232 207 240 480 60 1:2 240 212 LSD (P=0.05) 84 70 c v % 37 34 Sub treatments MgO (kg ha'1 yr1) 0 187 175 60 174 153 LSD(P=0.05) NS NS Generally, soil K levels increased with increasing rates of ground K fertilizer application at both depths. In this year, significant effect in soil K level was not found due to Mg fertilizer application at 60 MgO ha'1 yr 1, at both depths. 150 ANNUAL REPORT FOR 1997 TABLE 11 - Effect of different N:K20 ratios and MgO on soil Mg status at two soil depths Level (kg ha1 yr1) Soil Mg (mg kg') N KflMgO N:K20 0-15 cm 15-30 cm 240 48 0 5:1 165 120 240 48 60 5:1 210 160 240 60 0 4:1 182 187 240 60 60 4:1 202 183 240 80 0 3:1 254 172 240 80 60 3:1 220 214 240 120 0 2:1 185 171 240 120 60 2:1 217 171 240 240 0 1:1 213 160 240 240 60 1:1 193 199 240 360 0 2:3 198 141 240 360 60 2:3 218 213 240 480 0 1:2 196 199 240 480 60 1:2 190 212 LSD (P=0.05) NS NS cv% 39 40 Sub treatments MgO (kg ha :-' yr') 0 199 164 60 207 193 LSD(P=0.05) NS NS Significant differences in soil Mg levels were not observed either due to ground Mg fertilizer application at 60 kg ha? yr 1 or due to increased potash rates, at both depths, although differences exist with and with out Mg application. The effect of N:K.O ratios and MgO on leaf Mg and K is given in Table 12. ANNUAL REPORT FOR 1997 151 TABLE 12 - Effect of different N:K20 ratios and MgO on leafMg and K Level (kg ha1 yr') Leaf nutrient (%) N K20 (MgO) N:K20 K Mg 240 48 0 5:1 1.39 0.28 240 48 60 5:1 1.43 0.28 240 60 0 4:1 1.23 0.30 240 60 60 4:1 1.28 0.31 240 80 0 3:1 1.24 0.32 240 80 60 3:1 1.20 0.30 240 120 0 2:1 1.21 0.30 240 120 60 2:1 1.14 0.34 240 240 0 1:1 1.28 0.28 240 240 60 1:1 1.33 0.30 240 360 0 2:3 1.42 0.27 240 360 60 2:3 1.47 0.31 240 480 0 1:2 1.44 0.27 240 480 60 1:2 1.28 0.32 LSD (P=0.05) 0.202 0.03( cv% 12 10 Sub treatments MgO (kg ha -'yr') 0 1.32 0.29 60 1.31 0.31 LSD(P=0.05) NS 0.012 Magnesium concentration in the foliage significantly increased with the ground Mg fertilizer application at the rate of 60 kg ha-1 yr 1 . However, significant increases in leaf K concentrations were not observed with the increasing rates of potash. This experiment is in progress. R.G.A.Wijayawardhana, G.P.Gunaratne and L.S.K.Hettiarachchi 2.2.5. The effect of increasing levels of potash (in 100 increments) with N (in 100 increments) fertilizer, on soil/plant N/K status and yield of tea, StJames Estate, Hah ela - Clone TRI 2025 (1990). The yield obtained in the 2nd year of the 2nd cycle is given in Table 13. 152 ANNUAL REPORT FOR 1997 TABLE 13 - Effect of increasing levels of potash with N on yield of tea Level (kg ha' yr1) Yield N K20 N:K20 ratio (made tea kg ha'1 yr1) 100 100 1:1 2178 200 100 2:1 2165 300 100 3:1 2289 400 100 4:1 2299 500 100 5:1 2363 100 300 1:3 2319 200 300 2:3 2265 300 300 3:3 2363 400 300 4:3 2347 500 300 5:3 2344 100 500 1:5 2284 200 500 2:5 2363 300 500 3:5 2220 400 500 4:5 2385 500 500 5:5 2216 LSD (P=0.05) NS CV% 11 Level ofN (kg ha'1 yr1) 100 2261 200 2264 300 2291 400 2344 500 2308 LSD (P=0.05) NS Level ofKfl (kg ha1 yr1) 100 2259 300 2328 500 2294 LSD (P=0.05) NS Significant differences in yield were not observed between ground- application of N and K fertilizers with different N.K-O ratios. Further, there were no significant yield increases with the increasing rates of either N or K fertilizer, although the trends exist. The effects of N and potash ground applications on soil K status, and leaf N and K are given in Tables 14, and 15 respectively. ANNUAL REPORT FOR 1997 153 TABLE 14 - Effect of increasing levels of potash with N on soil K status at two depths Level ofN (kg ha'yr') Soil K (mg kg') 0-15 cm 15-30 cm 100 214 210 200 213 193 300 173 163 400 167 162 500 163 148 LSD (P=0.05) 34 31 cv% 27 27 Level ofK20 (kg ha' yr') 100 124 113 300 181 173 500 254 240 LSD (P=0.05) 26 24 cv% 27 27 TABLE 15 - Effect of increasing levels of potash with N on leafN and K status Level ofN (kg ha' yr' Leaf nutrient (%) N K 100 3.27 1.58 200 3.26 1.49 300 3.48 1.50 400 3.45 1.49 500 3.52 1.46 LSD(P=0.05) 0.175 0.094 C V % 8 9 Level ofKp (kg ha' yr') 100 3.38 1.41 300 3.40 1.53 500 3.41 1.57 LSD (P=0.05) NS 0.073 In general, soil K levels decreased with the increasing rates of N at both depths, and increased with the increasing rates of potash. Leaf N concentration gradually increased with the increasing rates of N while leaf K concentration decreased. LeafN concentration remained unaffected with the increasing rates of potash, while leaf K concentration increased. This experiment is in progress. R.G.A.Wijayawardhana, G.P.Gunaratne and L.S.K.Hettiarachchi 154 ANNUAL REPORT FOR 1997 2.3. Soil and Fertilizer Phosphorus Studies. 23 .1 . Application of increasing levels of phosphate (0-120 kg P 2 0 5 ha 1 yr 1 at 20 kg increments) fertilizer on soil/plant P status and yield of tea, St.Coombs Estate, Talawakele - Clone TRI 2025 (1989). The yield obtained in the 3rd year of the 2nd cycle and soil P levels are given in Tables 16 and 17 respectively. TABLE 16 - Effect of increasing levels of phosphate fertilizer on yield Level of P fertilizer Yield a O (kg ha1 yr' P ) (made tea kg ha-' yr') 0 2388 20 2384 40 2448 60 2432 80 2430 100 2387 120 2431 LSD(P=0.05) NS C V % 7 Significant increases in yield were not observed with the increasing rates of Eppawala rock phosphate(ERP). TABLE 17 - Effect of increasing levels of phosphate fertilizer on soil P Level of P fertilizer Soil P (mg kg') • ha'yr' P2Os) 0-15 cm 15-30 cm 0 23.3 24.7 20 15.2 9.2 40 26.7 33.4 60 34.3 20.8 80 55.0 44.4 100 43.2 28.5 120 51.6 38.4 LSD (P=0.05) 27.86 25.06 cv% 60 68 In general, soil P levels at 0-15 cm depth, extracted using borax solution (pH= 1.5) increased with the increasing rates of ERR However, no overall partem of increase was observed at 15-30 cm depth. This experiment is in progress. H.A.P.Warnasiri, A.K.N.Zoysa and L.S.K.Hettiarachchi ANNUAL REPORT FOR 1997 155 Levels of P fertilizer Yield (kgha'yr* P205) (made tea kg ha1 yr1) Surface Incorporated 0 5414 5510 20 5231 5101 40 6112 5128 60 5424 5374 80 5642 5050 100 4834 5408 120 5953 5443 LSD(P=0.05) NS cv% 12 Significant differences in yield were not observed due to either the rates of P or mode of application i.e. surface applied or incorporated. This experiment is in progress. H.A.P.Warnasiri, A.K.N.Zoysa and L.S.K.Hettiarachchi 23.3. Effect of foliar application of phosphorus (0, 1, 2, 3 and 4% DAP and TSP) on plant P status, quality and yield of tea, St.Coombs Estate, Talawakele - Clones DT 1 and CY9 (1992). The yield obtained in the 4th year of the 1st cycle (i.e. from December 1996 to November 1997) is presented in Table 19. 23.2. Application of increasing levels of phosphate fertilizer with two methods of application (Broadcast and Incorporated) on soil/plant P status and yield of tea, Walahanduwa Estate, Galle - Clone TRI 2025 (1994). The yield obtained in the 3rd year of the 1st cycle is given in Table 18. TABLE 18 - Effect of increasing levels of surface applied and soil incorporated ERP on yield 156 ANNUAL REPORT FOR 1997 TABLE 19 - Effect of foliar application of phosphate on yield Treatments Yield (made tea kg ha' yr') Clone CY9 DTI Control 2602 2310 1 % TSP 2678 2466 2 % " 2937 2373 3 % " 2681 2341 4 % " 2805 2358 1 % DAP 2586 2304 2 % " 2780 2572 3 % " 2581 2338 4 % " 2538 2328 LSD (P=0.05) NS NS cv% 7 3 Significant clifferences in yield were not observed due to foliar application of P in either form(i.e. TSP or DAP), compared to control. After modifying, foliar phosphorus concentrations in September 1995, at different times, flush samples were subjected to miniature manufacture and assessed for P concentrations and biochemical parameters of made teas, and as well as for P concentrations of leaves. Data interpretation and the other day-to-day operations are in progress. R.G.A.Wijayawardhana and L.S.K.Hettiarachchi 2.4. Soil and Fertilizer Magnesium Studies 2.4.1. Application of increasing levels of kieserite on soil/plant nutrient status and yield of tea, StCoombs Estate, Talawakele - Clone TRI 2025 (1990). The yield obtained in the 1st year of the 2nd cycle is given in Table 20. TABLE 20 - Effect of increasing levels of kieserite application on yield Level of kieserite Yield (kg ha' yr' MgO) (made tea kg ha' yr') 0 2485 15 2455 30 2440 45 2434 60 2315 75 2263 LSD (P=0.05) cv% NS 9 ANNUAL REPORT FOR 1997 157 Significant differences in yield were not observed due to application of different rates of kieserite. The effect of kieserite on soil pH levels is given in Table 21. TABLE 21 - Effect of increasing levels of kieserite on soil pH at two soil depths Level of kieserite Soil pH (kg ha-1 yr1 MgO) 0-15 cm 15-30 cm 0 4.83 4.75 15 4.53 4.61 30 4.66 4.78 45 4.57 4.62 60 4.61 4.54 75 4.51 4.56 LSD (P=0.05) 0.284 NS cv% 4 5 Significant differences were not observed in soil pH levels at both depths, due to different rates of kieserite. This experiment is in progress. H.A.P.Warnasiri and L.S.K.Hettiarachchi 2.4.2. Application of increasing levels of dolomite fertilizer at three frequencies (Cycle, Mid & yearly basis) on soil/plant nutrient status and yield of tea, Field No.4, StCoombs Estate, Talawakele - Clone TC 9 (1989). The yield obtained in the 2nd year of the 2nd cycle is given in Table 22. TABLE 22 - Effect of increasing levels of dolomite application on yield Level of dolomite Yield (made tea kg ha1 yr1) Frequency of dolomite application (kg ha1) Cycle Mid-cycle Yearly Control 3102 3102 3102 1250 3087 3057 3193 2500 3000 3149 3214 5000 2889 2936 2950 10000 2743 2897 - LSD (P=0.05) 304 cv% 8 158 ANNUAL REPORT FOR 1997 Lower yield was recorded from plots that received dolomite at the rate of 10,000 kg ha-1 pruning-cycle_1, at cycle frequency when compared to control. This experiment is in progress. H.A.P. Warnasiri and L.S.K.Hettiarachchi 2.4.3. Application of increasing levels of dolomite fertilizer at three frequencies (Cycle, Mid & Yearly basis) on soil/plant nutrient status and yield of tea, Field No.2, Morogolla Estate, Imaduwa - Clone TRI 2025 (1990). The yield obtained in the 1st year of the 3rd cycle is shown in Table 23. TABLE 23 - Effect of increasing levels of dolomite application on yield Level of dolomite Yield (made tea kg ha' yr') Frequency of dolomite application (kg ha') Cycle Mid-cycle Yearly Control 1965 1965 1965 1000 2484 2388 2304 3000 2379 2153 2306 5000 2164 2075 2392 LSD (P=0.05) NS cv% 11 Significant differences in yield were not observed either due to level or frequency of dolomitic limestone application. However, lower yield was observed from the control plots where no dolomite was applied over a period of 7 years. This experiment is in progress. H.A.P.Warnasiri and L.S.K.Hettiarachchi 2.4.4. Effect of different particle sizes of applied dolomite fertilizer on soil pH, soil/plant Mg status and yield of tea, Mattakelle Estate, Talawakele - Clone TRI 2023 (1991). The plants in these plots were pruned in April 1996, and have recovered after a long period of time due to blister blight attacks. Hence, plants were harvested only for a period of 2 months in the 1st year of the 2nd cycle. Therefore, computation of statistics in relation to yield data was not carried out. The soil Mg levels are presented in Table 24. ANNUAL REPORT FOR 1997 159 TABLE 24 - Effect of different sizes of dolomite particle on soil Mg levels Particle size combinations of dolomite Passing through: 30 BS Passing through: 100 BS Depth (cm) 0-15 15-30 LSD (P=0.05) 0-15 cm cv% LSD (P=0.05) 15-30 cm cv% 100 100 100* 100 100 100 75 50 25 0 Soil Mg (mg kg') 44.5 42.8 43.5 37.0 35.5 50.8 48.3 47.5 38.3 50.3 NS 32 NS 32 * Presently recommended particle size of dolomite fertilizer In general, significant differences in soil Mg levels were not found due to application of dolomitic limestone with different particle size combinations tested, at both depths. This experiment is in progress. H.A.P.Warnasiri and L.S.K.Hettiarachchi 2.4.5. Effect of different particle sizes of applied dolomite fertilizer on soil pH, soil/plant Mg status and yield of tea, Talangaha Estate, Nakiyadeniya - Clone TRI 2025 (1991). The yield obtained in the 3rd year of the 2nd cycle and the soil Mg levels are presented in Tables 25 and 26 respectively. TABLE 25 - Effect of different sizes of dolomite particles on yield Particle size combinations of dolomite Passing through: 30 BS 100 100 100* 100 100 Passing through: 100 BS 100 75 50 25 0 Yield (made tea, kg ha' yr') 3092 3183 3216 3212 3234 LSD (P=0.05) NS CV % 9 * Presently recommended particle size of dolomite fertilizer At this site, significant differences in yield were not observed due to application of dolomitic limestone with different particle size combinations. 160 ANNUAL REPORT FOR 1997 TABLE 26 - Effect of different sizes of dolomite particle on soil Mg levels Particle size combinations of dolomite Passing through: 30 BS 100 100 100* 100 100 Passing through: 100 BS 100 75 50 25 0 Soil Mg (mg kg') 0-15 104.3 67.3 93.8 92.8 104.3 15-30 81.8 52.5 81.8 50.8 61.5 LSD (P=0.05) 0-15 cm NS CV % 32 LSD (P=0.05) 15-30 cm 25.8 CV % 30 * Presently recommended particle size of dolomite fertilizer In general, significant differences in soil Mg levels at 0-15 cm depth were not found due to application of dolomitic limestone with different particle size combinations, although at 15-30 cm depth, some differences were greater than the LSD value. This experiment is in progress. H.A.P.Warnasiri and L.S.K.Hettiarachchi 2.4.6. Effects of application of potassium and/or magnesium from Sul-Po-Mag and kieserite at 2 levels of N on soil/plant nutrient status and yield of tea, Kiruwanaganga Estate, Galle - Clone TRI 2025 (1993). The yield obtained in the 1 st year of the 2nd cycle and the soil Mg levels are presented in Tables 27 and 28 respectively. TABLE 27 - Effect of application of K and/or Mg from kieserite and Sul-Po-Mag on yield Treatments Yield (made tea kg ha' yr') Level of Nitrogen (kg ha' yr') 240 360 U709 (Urea) 2457 2558 U 709 + Kieserite 2576 2458 U 750 Sul-Po-Mag 2471 2362 UT Mix. (Urea & SA) 2613 2340 T 1130 (SA) 2693 2737 LSD (P=0.05) NS cv% 10 Significant yield differences were not found either due to application of different fertilizer mixtures or between 2 N levels. ANNUAL REPORT FOR 1997 161 TABLE 28 - Effect of application of potassium and/or magnesium from kieserite and Sul-Po-Mag on soil Mg at 0-15 and 15-30cm depths Treatments Soil Mg (mg kg1) N level (kg ha1 yr')0-15 cm 15-30 cm U709 (Urea) 240 54.3 39.2 U 709 + Kieserite 240 65.1 61.4 U 750 Sul-Po-Mag 240 58.6 36.1 UT Mix.(Urea & SA) 240 44.6 23.9 T 1130 (SA) 240 23.7 16.3 U709 (Urea) 360 50.2 43.0 U 709 + Kieserite 360 81.8 58.5 U 750 Sul-Po-Mag 360 52.6 58.7 UT Mix. (Urea & SA) 360 34.4 19.0 T 1130 (SA) 360 28.5 23.7 LSD (P=0.05) 34.8 52.69 cv% 49 96 In this year, although significant differences in soil Mg levels at both depths were not observed due to addition of Mg enriched NPK fertilizer mixtures at both levels of N, considerably lower levels were found particularly from the plots that received Til30 mixture, which is probably due to lower soil pH values, even after dolomitic limestone application at the time of pruning. This experiment is in progress. S.M.Dissanayake and L.S.K.Hettiarachchi 2.4.7. Effects of application of potassium and/or magnesium from Sul-Po-Mag and kieserite with and without dolomite on soil/plant nutrient status and yield of tea, Hopton Estate, Passara - Clone TRI 2025 (1993). The yield obtained in the 4th year of the 1st cycle is presented in Table 29. TABLE 29 - Effect of application of potassium and/or magnesium from kieserite and Sul-Po-Mag on yield Treatments Yield (made tea kg ha1 yr1) Without dolomite With dolomite U709 (Urea) 1698 1637 U 709 + Kieserite 1691 1667 U 750 Sul-Po-Mag 1780 1630 UT Mix. (Urea & SA) 1725 1657 T1130 (SA) 1665 1730 LSD (P=0.05) cv% NS 6 162 ANNUAL REPORT FOR 1997 S.M.Dissanayake and L.S.K.Hettiarachchi 2.4.8. Effect of application of potassium and/or magnesium (U 709, U 709+Kie, U 750, UT Mix & Tl 130 & 2 levels of N - 240 & 360 kg ha"1 yr 1) from Sul-Po-Mag and kieserite on soil/plant nutrient status and yield of tea, Waltrim Estate, Lindula - Clone TRI 2025 (1994). The yield obtained in the 3rd year of the 1st cycle and the soil Mg levels are presented in Tables 30 and 31 respectively. ' TABLE 30 - Effect of application of potassium and/or magnesiumfrom kieserite and Sul-Po-Mag on yield Treatments Yield (made tea kg fur' yr') Levels of Nitrogen (kg ha' yr') 240 360 U709 (Urea) 3249 3265 U 709 + Kieserite 2925 3438 U 750 Sul-Po-Mag 3090 3522 UT Mix. (Urea & SA) 3168 3199 T1130 (SA) 3303 3438 LSD (P=0.05) NS C V % 6 Yield (made tea kg fur' yr') Levels ofN (kg ha' yr') 240 3147 360 3372 LSD (P=0.05) 149 So far, significant effect in yield was not found between these fertilizer- treatment and N levels. Also, significant yield differences were not found due to application of different fertilizer mixtures alone. However in this year, yield significantly increased due to application of N at 360 kg ha-1 yr 1 when compared to application of N at 240 kg ha 1 yr 1 . Significant differences in yield were not observed either due to any of these fertilizer-treatments or to with and without dolomite applications. This experiment is in progress. ANNUAL REPORT FOR 1997 163 TABLE 31 - Effect of application ofpotassiumand/or magnesiumfrom kieserite and Sul-Po-Mag on soil Mg at 0-15cm depth Treatments Soil Mg (mg kg1) N Level (kg ha1 yr1) 0-15 cm 15-30 cm U709 (Urea) 240 15.7 21.5 U 709 + Kieserite 240 24.6 27.9 U 750 Sul-Po-Mag 240 32.2 37.2 UT Mix. (Urea & SA) 240 15.4 23.1 T 1130 (SA) 240 10.7 12.5 U709 (Urea) 360 14.5 17.5 U 709 + Kieserite 360 29.6 30.6 U 750 Sul-Po-Mag 360 36.3 41.3 UT Mix. (Urea & SA) 360 11.6 10.0 T 1130 (SA) 360 14.7 16.9 LSD(P=0.05) NS NS cv% 35 42 SoilMg (mg kg1) 0-15 cm 15-30 cm U709 (Urea) 15.1 19.5 U 709 + Kieserite 27.1 29.3 U 750 Sul-Po-Mag 34.2 39.3 UT Mix. (Urea & SA) 13.5 16.6 T 1130 (SA) 12.7 14.7 LSD (P=0.05) 8.68 12.15 So far, significant effect in soil Mg levels was not found between these fertilizer mixtures and N levels. Also, no significant differences in soil Mg levels were found between 2 levels of N. In this year, soil Mg levels significantly increased due to application of Mg enriched NPK mixtures such as U709 + kieserite and U750(Sul-Po-Mag) compared to NPK mixtures, yet the levels in general, are marginal. This experiment is in progress. S.M.Dissanayake and L.S.K.Hettiarachchi 164 ANNUAL REPORT FOR 1997 3. Project D/AGCH - Divisional activities 3.1. Characterization of soils in tea growing areas in Sri Lanka This project commenced in early July 1995 in collaboration with the Land Use Division of the Irrigation Department. Altogether, 12 soil pits were cut from the Ratnapura district from the identified soil types in different regions. A draft memorandum of understanding (MOU) is prepared and yet to be finalized, in order to carry out the work program. Another project was initiated in 1996 to study the land use planing options for development of marginal tea lands in mid country. Soil survey commenced in November 1996 in order to prepare detailed soil distribution maps through GIS software from 5 selected Estates, to incorporate soil information. The soils of West Hall, Hantane, Craighead, Galphelle and Ratwatte Estates were surveyed and samples are being analyzed for physico chemical parameters. Preparation of maps are in progress. The operations in this project have been carried out in collaboration with a soil surveyor f*">m the Environment and Forest Conservation Division of the Mahaweli Authority in Kandy, Agricultural Economics unit of the TRI and with a trained assistant cartographer. This project is partially funded by the Council for Agricultural Research Policy (CARP). WM.S.Wijethunge, G.P.Gunaratne, L.S.K.Hettiarachchi and J.A.A.M.Jayakody 3.2. Adaptive fertilizer trials - TRI & TSHDA collaboration The objectives of this study, are to evaluate the performance of U709 and T1130 fertiliser mixtures under tea small holders' situation with a view to disseminating technologies and convincing the small holder the advantages of U709 mixture. Soil pH levels prior to commencement of trials and after dolomitic limestone applications along with rates, obtained from 17 sites at tea small holders' lands, are presented in Table 32. ANNUAL REPORT FOR 1997 165 TABLE 32 - Soil pH levels prior to commencement of trials, after dolomitic limestone applications along with rates from 17 tea small holders' lands Soil pH at 0-15 cm depth Dolomite rate Prior to After dolomite (kg ha commencement application Block Block Location T1130 U709 T1130 U709 Boralanda 4.20 4.90 4.70 5.00 1500 Pitamaruwa 4.70 4.70 4.90 5.10 1000 Kandagolla 4.70 4.80 4.80 4.90 1000 Balangoda 3.50 3.50 3.70 3.80 2000 Kalawana 3.70 3.65 4.20 4.30 2000 Deraniyagala 3.80 3.90 4.50 4.30 2000 Ingiriya 4.55 4.50 4.50 5.20 2000 Badureliya 3.40 4.00 3.80 4.00 2000 Kadugannawa 4.10 4.10 4.30 4.40 1500 Rangama 4.20 4.30 4.50 4.50 1500 Hiniduma 3.70 3.60 4.40 4.40 2000 Elpitiya 4.10 4.40 4.70 5.00 2000 Akuressa 3.80 3.80 4.10 4.00 2000 Urubokka 4.10 4.20 4.50 5.10 2000 Deniyaya 3.60 3.80 4.80 5.50 2000 Tispane 4.30 4.30 4.30 4.30 1500 Nortonbridge 4.50 4.60 4.50 4.50 1000 In general, pH levels were found to be below 4.5 and in some plots it was below 4.0, prior to commencement. The levels were determined after 4 to 6 months period. After dolomite applications, pH levels improved except at few sites. This is probably due to site specific characters such as slope, type of soil etc. The trial established at Rikillagaskada was abandoned due to unavoidable circumstances and instead another trial was established in the same area recently. The field operations such as fertilizer applications etc. are in progress. These trials are carried out in collaboration with the staff members of both the TRI's Advisory and Extension stations and Tea Small Holdings Development Authority, in those respective regions. H.A.P.Warnasiri, S.M.Dissanayake, W.M.S.Wijethunge, G.P.Gunaratne, L.S.K.Hettiarachchi, and M.B.A.Perera, S.L.D.Amarathunge, RB.Ekanayake, S.T.Yatawatte, K.D.Dahanayake, J.A.S.K.V.Jayasinghe, S.Sithakaran and C.C.Rajasingham 166 ANNUAL REPORT FOR 1997 3.3. High Forest Estate Problem Clone TRI 2025 - Die back of shoots The details of the problem were given in the Annual Report for 1994. As per the proposals made at High Forest Task Force meetings, two micro plots (each consisting of 20 plants) were demarcated from the affected patch, in order to apply both ground and foliar fertilizers. Previously marked plot (consisting of 20 plants) from the unaffected patch was used as the control. During 1997,06 applications of both ground (N, P, K, Mg & S) and foliar (N, P, K, Mg, S, B, Mo, Cu & Fe) fertilizer were given together. Henceforth, improvement in relation to general growth was observed. Experiments are in progress as per proposals. S.Ananthacumaraswamy and L.S.K.Hettiarachchi 3.4. Soil and Plant Sulphur Survey The work in relation to this study was completed, and will be published. S.Ananthacumaraswamy and L.S.K.Hettiarachchi 3.5. Dolomite cum fertilizer applicator The objective of this study is to see whether presently available motorized and/or hand-operated fertilizer/granule applicators can be modified in order to broadcast dolomite and/other fertilizer mixtures for tea plantations. A motorized applicator available in the market was tested at St.Coombs with dolomite and fertilizer mixtures for its suitability, and found that this type of applicators, were not suitable to broadcast either dolomite or fertilizer mixtures. The particle size distributions of a range of fertilizer mixtures that are used in the tea sector were obtained, in order to prepare specifications and requirements to purchase a power-operated applicator for testing. The specifications were prepared and forwarded to local agricultural machinery agencies for the submission of suitable applicators from the manufacturers. This is in progress. S.M.Hulangamuwa and L.S.K.Hettiarachchi 3.6 IPM/SHB Experiments As per the proposals made by SHB Task Force, two nursery experiments commenced at Hantane station and Attampitiya Estate, to test the effects of ANNUAL REPORT FOR 1997 167 high potash (KjCOlevels on SHB infestation. The plants raised were removed at different time intervals, and soil and leaf samples are being analyzed for possible growth, and soil and plant nutrient parameters. Thereafter, treated plants that remained on nurseries after the completion of respective periods, were transplanted in the field, in order to investigate the effects on immature plants, as per designed trials. These experiments are in progress. H.A.P.Warnasiri, S.M. Dissanayake, W.M.S.Wijethunge, G.P.Gunaratne and L.S.K.Hettiarachchi 4. Project C/ANAL - Central Analytical Services The number of fertilizer, soil and leaf samples analyzed for advisory purposes during 1997 are given below: Element Fertilizer Soil Leaf Total Nitrogen 860 472 22 1354 Phosphorus 753 913 327 1993 Potassium 825 1228 327 2380 Magnesium 560 1108 327 1995 Calcium 02 08 04 14 Sodium - 08 - 08 Zinc 42 44 411 497 Copper - 08 23 31 Iron - 08 04 12 Manganese - 08 04 12 Biuret 03 - - 03 E.C - 02 - 02 PH - 2504 - 2504 O.C% - 2524 - 2524 Mesh size 370 - - 370 Total 3415 8835 1449 13,699 G.P.Gunaratne, S.Ananthacumaraswamy, R.G.A.Wijayawardhana, W.M.S.Wijethunge, H.A.P.Warnasiri, S.M.Dissanayake and L.S.K.Hettiarachchi 5. Meetings and Seminars Dr L.S.K. Hettiarachchi, Mrs S. Ananthacumaraswamy, Mr R. G. A. Wijayawardhana attended a workshop on "Safe Handling of Pesticides" organized by Sri Lanka Crop Protection Association, held at TRI Auditorium on 22nd January. 168 ANNUAL REPORT FOR 1997 Dr L.S.K.Hettiarachchi and Mrs S.Ananthacumaraswamy attended the 194th meeting of the Experiments and Extension forum held at TRI Auditorium on 24th January. Dr L.S.K.Hettiarachchi attended a Technical Committee on Tea meeting at Sri Lanka Standards Institution, in order to discuss matters in relation to forthcoming 17th ISO Technical Committee on Tea meeting to be held in Colombo, during 17th - 19th February, on 31st January. Dr L.S.K.Hettiarachchi attended a seminar on Land Degradation in Sri Lanka, organized by MPI/ACA1R held at TRI Hantane station on 11th February. Dr L.S.K.Hettiarachchi attended the 17th ISO Technical Committee on Tea meeting held at Trans Asia Hotel in Colombo from 17th to 19th February. Dr L.S.K.Hettiarachchi attended the RSC (Nuwara Eliya) meeting held at TRI Auditorium on 27th February. Dr L.S.K.Hettiarachchi participated at the seminar organized by Lanka Phosphate Ltd, in the promotion of ERP in tea small holding sector, held at the HARTI in Colombo on 31st March and addressed on the "Present recommendations on the use of ERP in tea and their significance" as well. Dr L.S.K.Hettiarachchi addressed the members of the Planters Association Sub district meeting on "Management of Tea Soil Fertility in Nuwara Eliya sub-district" at Planters Club, Talawakele on 6th May. Mrs S.Ananthacumaraswamy addressed the members of RSC III in Uva on "Use of Fertilizer to Increase the Land Productivity in Uva Region" at Bandarawela Tennis Club, Bandarawela on 13th June. Dr L.S.K. Hettiarachchi joined the Director, TRI in a discussion with Messrs. M.J. Albrecht and A. Munasinghe from IMC Global Operations Inc, U.S.A. and Aristons Pvt. Ltd., Colombo respectively, to discuss the progress in relation to Sul-Po-Mag trials on 24th June. Dr L.S.K.Hettiarachchi addressed the members of the 195th E & E forum on "Experimental Evidence: Towards Revised Fertilizer Recommendations" at TRI Auditorium, Talawakele on 27th June. The Executive staff members of the division also attended the forum. Dr L.S.K.Hettiarachchi attended a symposium on "Upper Mahaweli Catchment Conservation - Status & Trends", jointly organized by the Environment & Forest Conservation Division of the Mahaweli Authority and Section B of SLAAS, held at SLAAS auditorium, Colombo on 04th July. ANNUAL REPORT FOR 1997 169 Dr L.S.K.Hettiarachchi participated at the International Seminar, organized by the Sri Lanka Tea Board on "Tea and Health", held at BMICH in Colombo from 14th to 15th August. Dr L.S.K.Hettiarachchi participated at the discussion, held between TRI Scientists and Executives of Kotagala, and Agrapatana Plantations Company Ltd, at TRI Auditorium, Talawakele on 29th August. Dr L.S.K.Hettiarachchi addressed the members of the RSC-I in up- country region on "General nutrient management in tea" on 04th September at TRI Auditorium, Talawakele. Dr L.S.K. Hettiarachchi participated in a panel discussion of a RSC seminar in Kandy on 18th September. Dr L.S.K.Hettiarachchi participated at a meeting organized by Dr. A.N. Jayakody, Senior Supervisor of Mr G.P.Gunaratne's postgraduate studies, attached to Soil Science Department, University of Peradeniya in order to discuss the progress so far made by the candidate, with a view towards the completion, on 18th September. Dr L.S.K.Hettiarachchi along with DGM (Extension), TSHDA attended the 1st set of progress meetings arranged between TRI and TSHDA officials, in order to discuss problems and constraints in relation to monitoring 18 collaborative fertilizer trials established at tea small holders' lands and thereby to improve monitoring. Dr L.S.K.Hettiarachchi attended the Annual General Meeting of the Soil Science Society of Sri Lanka at SLAAS Auditorium on 10th October and joined the field visit to Horton plains on 11th October. Dr L.S.K.Hettiarachchi along with Dr S.M.Hulangamuwa visited Farm Machinery Research Centre at Maha-Hlupallama and Farm Machinery Training Centre at Anuradhapura, in order to see some applicators available at these centers; discuss the possibilities of fabricating a hand-operated applicator; explain the requirements under tea crop situation and to meet the Agricultural Engineers and thereby to familiarize him self with calibration procedures etc. from 16th to 19th October Dr L.S.K. Hettiarachchi participated at a discussion organized by the senior officials of the Pussellawa Plantations Ltd, at the Hellbodde Estate, in order to prepare it's fertilizer policy for 1998, on 11th November. Dr L.S.K.Hettiarachchi attended the 9th Annual Congress of the Post Graduate Institute of Agriculture at PGRC Auditorium in Peradeniya from 21 st to 22nd November. 170 ANNUAL REPORT FOR 1997 Dr L.S.K.Hettiarachchi participated at the panel discussion of a RSC seminar in Kandy on 11 December. 6. Workshops and Training programs Mr K.A.E.P.Fernando, NCA trainee from Technical College, Kuliyapitiya, completed his 04 months training program on soil, plant and fertilizer analyses, by January 1997. Mr J.M.A.R.Hemachandana, NCA trainee from Technical College, Kuliyapitiya, completed his 06 months training program on soil, plant and fertilizer analyses, from January to July 1997. Mr N.S.Athapattu and Mr W.C.Wickramarachchi, NDT trainees from Hardy Advanced Technical Institute, Ampara completed their 04 months training program on soil, plant and fertilizer analyses, from April to July 1997. Miss D.Liyanage NDT trainee from Hardy Advanced Technical Institute, Ampara and Mr C.Wickramasinghe NDT trainee from Technical Collage, Kuliyapitiya completed their 04 months training program on soil, plant and fertilizer analyses from August to November 1997. 7. Visitors Dr P.Owour, Senior Principal Research Officer/Head Chemist, Tea Research Foundation, Kenya, visited SPND in order to discuss tea soils and plant nutrient aspects in Sri Lanka, with Dr L.S.K. Hettiarachchi on 24th February. The managers from Glasgow and Balmoral Estates visited SPND in order to discuss general fertilizer related aspects, on 24th March. Three Group Managers from Agrapatana Plantations Limited visited SPND in order to discuss fertilizer related aspects with Dr L.S.K. Hettiarachchi on 23rd April. A group of students from Eastern University visited SPND in order to discuss and familiarize soil, plant and fertilizer related aspects, on 7th May. Mr M.C.Bostock, Executive Chairman, Rajawela Holdings Pvt. Ltd., visited SPND in order to discuss fertilizer related aspects with Dr L.S.K. Hettiarachchi, on 7th May. Four Assistant Superintendents from Plantations Management Companies visited SPND in order to discuss fertilizer related aspects and to familiarize soil, plant and fertilizer analytical work, on 16th and 19th May. ANNUAL REPORT FOR 1997 171 Mr M.de Zoysa from Chilaw Plantations Ltd., visited SPND in order to discuss fertilizer related aspects with Dr L.S.K. Hettiarachchi on 4th June. A group of agricultural students from Sabaragamuwa University visited SPND in order to discuss and familiarize soil, plant and fertilizer related aspects, on 16th July. Dr S.M.Hulangamuwa, Consultant Engineer, Department of Agriculture, Peradeniya visited SPND in order to discuss about dolomite and/or fertilizer applicator work and as well as to test one of the applicators available in the market on 7th August. Two Assistant Superintendents from Agalawatte Plantations Ltd, visited SPND in order to discuss fertilizer related aspects and to familiarize soil, plant and fertilizer analytical work, on 01st and 02nd September. Two groups of agricultural students from University of Peradeniya visited SPND in order to discuss and familiarize soil, plant and fertilizer related aspects, on 10th and 17th September. Messrs F.R.Amarasinghe and T.T.Christy from Maskeliya Plantations Ltd visited SPND to discuss modalities to lay down a collaborative fertilizer- related trial at Brunswick Estate, on 06th October. Dr A.N. Jayakody, a senior lecturer at the Soil Science dept: of the Faculty of Agriculture, University of Peradeniya visited SPND with a view to discuss a research component for a post graduate student with Dr L.S.K. Hettiarachchi and lay down necessary collaborative experiments, on 07th November. 8. Publications HETTIARACHCHI, L.S.K. AND GUNARATNE, G.P.(1997). Identification of magnesium deficiency symptoms : Leaf analysis as a guide in tea crop nutrition. TRI Up Date 2(1), 4. ZOYSA, A.K.N., LOGANATHAN, P. AND HEDLEY, M.J. (1997). A technique for studying rhizosphere processes in tree crops: soil phosphorus depletion around camellia (Camellia japonica L.) roots. Plant and Soil, 190: 253-265. HETTIARACHCHI, L.S.K., (1997). Thea Vagave Sadhaha Pohora In Phohara Veladunta Athvelak, published by National Fertilizer Secretariat, Ministry of Agriculture and Lands, Colombo, Sri Lanka, 19-30. HETTIARACHCHI, L.S.K., BALASINGHAM, A., ANANTHA CUMRASWAMY, S., GUNARATNE, G.P. AND WARNASIRI, H.A.P. (1997). Mineral composition in relation to leaf maturity from 2000, 3000 and 4000 clonal series: Leaf analysis as a guide in tea crop nutrition. S.L.J. Tea Sci, 65 (1/2). In press. TECHNOLOGY DIVISION Head - M.T. ZiyadMohamed 1. General Mr. Robin Fundinger an engineer from IMKO, MICRO MODULTECHNIK, Germany spent one week from 1st April and tested a modified moisture meter to measure the moisture content in the withered leaf. Mr W.S. Botheju visited India from 22nd July to 31 st December to attend a training program on Tea Tasting and Quality Control at Kothari Agriculture Management Center in India. This visit was funded by the Technical Co­ operation Scheme of Colombo Plan. Mr. P.B. Chandradasa Experimental Officer, was transferred to Biochemistry Division with effect from 22nd September. Dr. M. T. Ziyad Mohamed served as a member of the Technical Committee appointed by Chairman Sri Lanka Tea Board to advice on Tea Factory Development Subsidy Scheme for orthodox factories. Dr. M. T. Ziyad Mohamed continued to serve as a member of the panel of Professional Examination in Tea Manufacture and Factory Practices by the Chairman, National Institute of Plantation Management (NJPM). Dr. M. T. Ziyad Mohamed was appointed to panel of Teachers/Examiners of the Post Graduate Institute of Science (PGIS) University of Peradeniya with effect from 4th July. Dr. M. T. Ziyad Mohamed was appointed to serve as the Chairman of the Technical Committee on Tea by the Sri Lanka Standard Institute. Dr. M. T. Ziyad Mohamed was appointed as a member of the Academic Committee of NTPM. Dr. M. T. Ziyad Mohamed was a member of the Sri Lankan delegation for the 17th Technical Committee on Tea Meeting of International Organization for Standards (ISO/TC34/SC8) held in Colombo in February. Dr. M. T. Ziyad Mohamed served as a member of the committee appointed by the Ministry of Plantation Industries on " Quality, Standards and Grades of tea produced and exported by Sri Lanka." Dr. M. T. Ziyad Mohamed continued to function as convenor/secretary of the Consultative Committee on Research, a Sub Committee of the Tea Research Board. ANNUAL REPORT FOR 1997 173 2. Project B/CPCO - Continuous process A continuous preconditioning system was designed by a University trainee in consultation with the divisional staff at Talawakele. The samples of material to fabricate the unit/system have been ordered. 3. Project B/EDRY - Reduction of the cost of tea drying 3.1 Performance of stepwise FBD 3 compared with FBD 4 Few trials were carried out from early May to compare the performance of FBD3. The objective is to optimize weir heights in three sections to increase the efficiency of the drier. This drier was designed in such a way that the weir heights in each section could be changed individually. Trials were restarted with presetting of weir heights at 4" as suggested earlier. TABLE 1 - Moisture content of tea in different sections and output of drier W/dhool Sections % Moisture Temperature(C) Average % Moisture 1st 2nd 3rd Inlet Weir end Output (Kg) (1) 53.5 31.5 6.0 2.4 129 112 194 52.8 31.0 7.7 2.2 129 112 52.9 33.1 10.1 3.1 117 107 52.5 36.0 9.2 3.2 116 104 (2) 55.5 31.0 8.2 2.8 112 104 197 54.9 38.6 18.2 4.0 115 91 55.8 44.9 25.2 4.6 123 94 The results revealed that the average output was about 195 kg on both occasions. The moisture contents of the samples drawn from each section were analyzed. The moisture content of dhool in each section is dependent on the initial moisture content of the fermented dhool, feeding rate as well as the inlet and exhaust temperatures of the dryer. This work is in progress. M. T. Ziyad Mohamed, W. S. Botheju, L. Jayasinghe 3.2 Trials with 'Economizers' Although the economizer was installed with necessary ducting, the amount of air sucked through economizer was not sufficient. Further modifications will be carried out and trials will continue. 174 ANNUAL REPORT FOR 1997 3.3 Solar Tea Drying Project The objective of this project is to test the feasibility of using solar energy for withering and drying. As an initial step two prototype solar flat plate collectors were designed and fabricated, and their performance tested at St Joachim Estate. The initial results showed that the air could be heated from about 32°C to 92°C using solar energy. To produce 1kg of tea, as much as 22 MJ of thermal energy is spent, according to energy audits carried out. The approach was to preheat the air which enters the heat exchanger to around 50 - 60°C using solar energy and to raise the temperatures to operational conditions (95°C) using firewood or oil. The technology using solar energy for thermal energy applications is well established. However, the importance of working out the economics would be looked into. Current Status: Fabrication of all 200 solar collectors was completed. The fabrication of ductings was also completed. The structure to fix the solar collectors has been decided and construction started with prestressed concrete columns and beams, in consultation with Dr. Kulasinghe, consultant for the project appointed by NARESA. S. Koneswaramoorthy, and M. T. Ziyad Mohamed 3.4 Dehumidified air technique for withering The objective is to check the effect of dehumidifying the air used for drying with a view to 1. Reduce the cost of drying by reducing the temperature of the drying air. 2. Check the improvement in tea character (if any). The miniature drier at the Technology Division was tested to find the initial condition for drying teas. Silica was packed in wire mesh and fixed to the drier to dehumidify the air used for drying. However the dehumidifying capacity of the silica packing was not satisfactory. The design of the silica packing was modified and this packing will be used in future tests. K. Raveendran, and M. T. Ziyad Mohamed In the meantime a cost effective prototype dehumidifying unit is being developed by Dr. Attalage of University of Moratuwa, for the specific purpose of using it for withering tea leaves. Once the unit is developed the Technology staff will get involved in testing the same, studying the effect of such a system ANNUAL REPORT FOR 1997 175 Wood losses in sawing / splitting 5 - 6% Labour for sawing (man-hrs/ cu.yd) 1 . 5 - 2 Labour for chopping (man-hrs/cu.yd) 6 - 1 0 Power consumption in sawing (Kwh/ cu.yd) 4 - 5 . 6 P.B.Chandradasa, G. L. C. Galahitiyawa, M. T. Ziyad Mohamed 4. Project B/ENGY - Reduction of Cost of Energy 4.1 Modifications to the trough with a view to reduce the cost of electri­ cal energy This is a collaborative project between TRI and Energy Conservation Fund (a statutory body under Ministry of Power and Energy) and it is funded by Energy Conservation Fund (ECF). Electrical energy is required for the unit operations of Withering, Rolling, Roll breaking and Drying. Electrical Energy consumption in sorting and packing on quality of made tea. This is a collaborative project with TRI, University of Moratuwa and Energy Conservation Fund (ECF). The project is funded by ECF. K. Raveendran, G. L. C. Galahitiyawa, M. T. Ziyad Mohamed 3.5 Generation of thermal energy using a local gasifier (NERD) for dry­ ing tea. The NERD gasifier was dismantled and a new one was fabricated with a thicker gauge B. I. sheet with modification to the burner unit at NERD work­ shop and installed during 4th quarter of 1996. The new unit was funded/paid by the Energy Conservation Fund. Trials were carried out for several days. Difficulties were encountered in raising the inlet temperature up to 200°F. On certain days it was possible to maintain an inlet temperature of around 200° F. This temperature although adequate but not the ideal for Low Country type of manufacture. Since the firewood has to be cut into small pieces to feed this gasifier, data on cost of splitting were also recorded. However, field trials would continue at least for another 6 months or so to make any recommendations. Wood-fuel losses and labour/power costs in splitting and chopping of fire­ wood Trials were carried out during the period under review and data col­ lected indicate the following. 176 ANNUAL REPORT FOR 1997 operation is rather small. The actual energy consumption in typical High/ Mid and Low country factories observed by analysis of historical data and by actual measurements during energy audits is given in Table 2. TABLE 2 - Electrical Energy Consumption in Tea Processing Unit Operation wise. Mid/High Country Low Country kWh/kg MT % kWh/KgMT % Withering 0.46 48.9 0.46 61.3 Rolling 0.20 21.3 0.10 13.3 Drying 0.07 7.4 0.07 9.3 Sorting & Packing 0.09 9.6 0.07 9.3 Ancillaries (including domestic use) 0.12 12.8 0.05 6.7 Total 0.94 100 0.75 100 (Ref W. C. A. De Silva,Planters Energy Network 1993) Bulk of electricity consumption takes place in the unit operation of withering and as the following analysis shows considerable energy savings could be effected by R + D relating to withering operations. In withering, electricity is consumed entirely by the fan motors operating continuously for about 12 hours in the case of Up/Mid country factories and about 18 hours in the case of Low country factories. For trough withering units the following design features are considered appropriate. Rate of spreading 30 Kg/m2 Air flow requirement 0.6 m3 /min/kg GL Required pressure head trough 15mm wg Estimated pressure loss in fan housing 5mm wg Fan head 20mm wg Assumed fan motor efficiency 80% Safety factor for motor capacity 25% Assumed fan efficiency 80% Based on the above, electrical energy requirements of a trough of 1,000 kg (GL) capacity is 4 kW and the corresponding specific electrical energy consumption (based on an outturn of 22.2% MT/GL is only 0. 22 kWh/kg MT for withers lasting 12 hrs and 0.32 kWh/kg MT for withers lasting 18 hrs, as against observed specific consumption of 0.46 kWh/kg MT. This clearly illustrates that the electricity consumption of trough fans is too high in relation to design figures. ANNUAL REPORT FOR 1997 177 Contributory factors often are incorrect fan auction design, lack of airflow distribution measures, use of oversize fans ( capacity being adjusted by fan blade angle), air leaks and the use of oversize motors. The extent of using oversize motors could be assessed by comparing the usually installed motor capacities (Samaraweera 1986) with those calculated to confirm to the design features given above. The details are given in Table 3. Usually installed motor capacities compared with design calculations based on an air requirement of 0.6m Vmin/kg GL for troughs of varying capacities TABLE 3 - Motor ratings for various trough sizes Trough (ft) 60 72 84 100 120 (ml 18.3 27.9 25.6 30.5 36.6 Capacity (kg) 988 1183 1382 1647 1976 Air flow required (m3/min) 593 710 829 988 1186 Air power @ 20mm total Pressure (kW) 1.93 2.31 2.70 3.22 3.86 Calculated fan motor Capacity (kW) 3.0 3.6 4.2 5.0 5.9 Calculated motor rating (kW)* 3.7 4.4 5.2 6.2 7.4 Usual motor rating 3.7 5.5 7.4 11 11 Oversize (%) 0 25 42 77 49 *(at an assumed motor efficiency of 65%, providing for a safety factor of 25%) By improving designs of troughs it should be possible to reduce electricity consumption in withering by 30% to 50% i.e. from 0.46 kWh to 0.32 kWh per kg of made tea. At a national level this represents a saving of about 28 GWh per annum. Methodology (1) To access the present energy consumption partem and to identify the energy saving potential, the following study methodology has been adopted. (2) Measure all the critical parameters to estimate the specific energy consumption of existing withering operation without making any operational changes. (3) Measure all the critical parameters while maintaining these parameters at desired range of withering in order to estimate the specific energy consumption. (4) Identify operational changes required for improvement of energy efficiency. (5) Carry out trials in the prototype withering trough to establish desired parameters to achieve optimum energy efficiency. 178 ANNUAL REPORT FOR 1997 Test Procedure Fresh green tea leaf was spread evenly on the trough at (2.5-3.2 kg/ft2). Spreading was done as quickly as possible and the trough fan started. Temperature measurements were taken at the following points. Air outlet over the leaf bed, Air inlet temperature, Ambient air position for hot air inlet duct and, Plenum chamber Temperature Wet bulb & Dry bulb temperature were monitored at four points using thermocouple wires of K type and a data logger and were continuously recorded at 15 minute intervals for the entire withering process. Weight Weight losses of green leaves during withering were measured by taking samples of 3.5kg green tea leaves contained in basket made out of wire mesh and placed on the same trough. The weights of these samples were recorded at one hour time intervals. Two samples of leaves were collected at the beginning and end of the withering process for measurement of moisture content. The total green leaf weight and the withered leaf weight (once the wither is ready) were also recorded. Static pressure Static pressure in the plenum chamber was also measured at 5 points along the length of the trough. Air flow Air velocity at inlet position was measured. In order to reduce the air flow rate through the bed the small door at the rear side of the trough (gable end door) was kept open from 4.00 a.m and air velocity through this back door was also measured. The leaf bed was turned twice during the process (11.30 p.m & 4.00 a.m). Observations and recommendations:- Table 4 gives the summary of the results of the field trials carried out at St Joachim Estate, Ratnapura. ANNUAL REPORT FOR 1997 TABLE - 4 Electrical Energy consumption during withering easel case2 case3 case4 case5* design condition Weight Distribution (kg/ft2) 2.5 2.5 3.14 2.5 3.2 2.8 Withering Time (h.min) 16.30 13.10 15.15 11.35 12.15 12 Specific Energy Consumption 0.316 0.316 0.256 0.241 0.201 0.222 (kWh/Tonne of MT) Initial Moisture Content % 74.01 77.7 80 75.7 80 Final Moisture Content % 65.00 65.0 65 61.2 60 * Controlled operation Moisture Loss Fig 1 shows the weight loss of leaves during the process. It was observed that more than 50% of the moisture removal has taken place during the first four hours of the withering process. Wet bulb/ Dry bulb Temperature Difference The withering process of the green leaves is ruled by the difference in wet and dry bulb temperature of the ambient air. The difference should be 2.2°C as an absolute minimum and the average around 3-4°C. During the wet days and in the evening the difference may be too small for withering to take place and in this case hot air from the drier is mixed with ambient air to achieve the required 'wet bulb depressions'. This is normally checked with dry and wet bulb thermometer in the trough. There is no meter to measure the moisture content in the leaf within a short period. It is common practice that the wither percentage is checked by squeezing the leaf by hand with the experience of the factory officer. During the measurements a continuous temperature recording unit was used with thermocouple wiring indicating the wet/dry bulb temperature of air entering and leaving air of the trough. Fig 2 shows the variation of wet & dry bulb temperature difference under normal operation and Fig 3 shows the wet/dry bulb temperature difference under controlled conditions. The specific energy consumption at the persent operational mode is about 0.316kWh/KgofMT. This value was reduced to 0.241 at moderately controlled conditions. Based on the above findings it could be concluded that energy consumption for withering could be reduced at least by 20% with proper control of Wet/Dry bulb temperatures. 180 ANNUAL REPORT FOR 1997 To carry out further trials a prototype trough (20'X6') with all the necessary ducting, was constructed at the Mechanical Workshop and the fan and motor were coupled/ installed to the same unit. Based on the above findings a speed controller will be installed to control the airflow with time of withering M.T. Ziyad Mohamed, G. L. C. Galahitiyawa and ECF Officials (L Project D/TECH - Divisional activities 6.1 Testing new moisture meter to measure the moisture content in withered leaf TRIME-GM (Time domain Reflectometry with Intelligent microelements for Gravimetric Water content measuring) based on the TDR- technique was tested during the year 1996. A modified instrument brought by an Engineer from IMKO, MICROMODULTECHNIK, Germany was tested with withered leaf samples with varying moisture content during the year 1997. Graph A, TRTME Mass% (instrument reading) vs Reference value in Mass% (result from standard laboratory test) was obtained by the Engineer from the test results. The test was done using withered leaves consisting of, 1) Mixed leaves (tender leaves + coarse leaves) 2) Tender leaves In the graph it can be seen that the points are scattered. Further to this the meter was tested by the officers attached to Technology division on several occasions and similar graphs were obtained. Based on these test results the following conclusions were made. a) The results are encouraging for samples with moisture levels below 60% b) The results are not reliable when, (i) The moisture content in the sample is above 60% (ii) There is more moisture in the stem compared to leaves during rainy days. It is difficult to remove the excess moisture and the moisture content of the withered leaf varies. (iii) The leaf standard is poor (iv) There is presence of sand This is a collaborative project with Post Harvest Technology Department, University of Hohenheim, Germany. M. T. Ziyad Mohamed, K. Raveendran, S. H. Priyanthi, L. Jayasinghe ANNUAL REPORT FOR 1997 181 6.2 Optimization of grade mix - No3 mesh for the Roll Breaker The objective of this study is to check the merits and demerits of using No 3 mesh instead of No 4 mesh in the roll breaker for low country type of manufacture. Since the perforation is larger in No 3 mesh higher dhool percentages were expected. 550 kg of withered leaf was charged initially to two rollers with 4x20 minutes rolling. The dool percentages are presented below. Mesh No. 4 (%) (single roll breaking) 1st dhool 2.20 2nd dhool 5.00 3rd dhool 22.30 (double roll breaking) 4th dhool 24.20 (No. 2 mesh single roll breaking) Big Bulk 46.20 s (%) B0P1(33) 8.8 BOP(40) 5.8 OP1 5.2 OP 13.1 FBOP 7.3 FBOPF1 4.3 FBOPF 3.1 F PEKOE 1.8 OPA(25) 12.6 OPA(30) 5.0 FFsp 1.2 OFF GRADES 24.1 Labour requirement for roll breaking : 5 nos' From the above it could be seen that the dhool percentages with No. 4 mesh were less and big bulk percentage was high. As such it was decided to do double roll breaking in the case of No. 4 roll breaker. This is a common practice in most of the Low Country factories. The 1SI 2 n d and 3rd dhools were extracted by roll breaking the bulk twice (double roll breaking) in the case of control using No 4 mesh. In the case of treatment using No 3 mesh, the 1 s t 2 n d and 3 r d dhools were extracted by roll breaking the rolled leaf once. The 4 l h dhool was extracted using a No 2 roll breaker mesh in each case. Three replicates were completed during the period under review and the results are summarized below 182 ANNUAL REPORT FOR 1997 TABLE 5 - Dhool percentages using No. 3 mesh verses No. 4 mesh in the roll breaker mesh no.3 mesh no.4 (single roll breaking) % (double roll brea] % I s' dhool /c 8.48 /V 7.50 2 n d dhool 13.84 16.53 3 r d dhool 21.60 21.21 4 t h dhool 32.11 26.24 Big Bulk 23.91 28.50 Grades (%) (%) BOPl(33) 9.0 9.8 BOP1(40) 7.1 7.0 OP1 12.3 11.0 OP 11.6 11.4 FBOP 8.2 8.6 FBOPF1 3.5 4.3 FBOPF 4.2 3.1 F PEKOE 2.9 2.5 PEKOE 12.0 10.0 OPA(25) 8.0 7.8 OPA(30) 4.1 6.0 FFsp 1.8 1.9 OFF GRADES 15.6 16.6 Labour requirement for roll breaking: 5 no.s' 10 no.s' Preliminary results indicate that the dhool percentages and grade percentages are comparable, in spite of employing single roll breaking with No. 3 mesh against double roll breaking with No. 4 mesh. However, the samples were also sent to professional tasters for their evaluation. If the valuation is comparable or better in the case of treatment using No. 3 mesh, this would result in substantial reduction in deployment of labour in the rolling room as mentioned above i.e. 5 labourers against 10. This is in progress. G.L.C. Galahitiyawa, P.B. Chandradasa, N. Vidanapathirana, M.T. Ziyad Mohamed 6.3 Net Outturn - made tea to green leaf. Net outturn achievable under low country conditions had been the subject of debate for some time. This is an important factor for the leaf suppliers, mainly small holders as well as factory owners since the price paid for the green leaf is based on this outturn. ANNUAL REPORT FOR 1997 183 Trials were carried out under commercial conditions at St Joachim Factory, Ratnapura, where more than 90% of the production is from bought leaf. Several batches of green leaf were selected, and manufacturing was carried out under normal (commercial) conditions. The teas were graded and the net outturn determined for each batch. The results from 18 replicates are summarized in Table 6. TABLE 6 - Gross and net outturn percentages in made tea when the good leaf percentage is over 60 Rep. Moisture Good leaf Gross Refuse tea Net No. content(%) (%) outturn(%) (%) outturn(%) 1 79.06 83.00 22.18 2.22 19.65 2 79.11 80.50 21.31 2.68 20.25 3 76.19 73.50 23.82 3.09 22.80 4 77.83 86.70 21.39 1.98 20.41 5 80.84 84.60 19.75 0.57 19.47 6 79.42 77.50 19.67 1.18 19.08 7 74.73 85.27 24.86 1.60 24.55 8 77.00 73.60 22.60 3.68 22.21 9 78.00 62.00 21.65 5.73 20.99 10 77.18 66.20 22.30 3.96 22.46 11 76.23 72.80 23.65 1.80 23.22 12 74.50 70.00 24.88 2.72 23.97 13 77.56 70.27 22.75 4.28 22.17 14 79.90 71.65 20.27 4.13 18.30 15 76.80 71.83 23.28 5.13 22.48 16 77.80 72.55 22.38 5.24 21.08 17 74.60 71.40 24.80 4.84 22.69 18 79.00 49.00 21.47 4.98 19.41 1) The regression for Net Outturn X Moisture Content of green leaf Predictor coef St dev t-ratio Probability Constant 91.833 7.135 12.87 0.000 MC -0.90833 0.09199 -9.87 0.000 *** S = 0.6979 R 2 = 85.9% R 2 (adjusted) = 85.0% *** Highly significant - 85% of the variation of Net outturn is explained by Moisture content of green leaf. The regression equation is NO = 91.8 - 0.908MC (1) NO - Net outturn, MC - Moisture content of green leaf. 184 ANNUAL REPORT FOR 1997 2) The regression for Net outturn X Good leaf% gave NO = 21.2 + 0.0024 GL (2) Predictor Coef Stdev t-ratio Probability Constant 21.227 3.660 5.80 0.000 goodleaf 0.00235 0.04947 0.05 0.963 **** s = 1.859 R - S Q = 0.0% R - SQ(ADJ) = 0.0% **** Not significant - Net outturn has no relationship with Good Leaf% This implies that the nett outturn does not depend on good leaf percentage 3) The regression for Net outturn, moisture content & good leaf (GL) NO = 91.2 - 0912 MC + 0.0128 GL (3) Predictor Coef Stdev t-ratio Probability Constant 91.172 7.325 12.45 0.000 mc -0.91191 0.09375 -9.73 0.000*** gl 0.01278 0.01893 0.68 0.510 NS s = 0.7101 R-sq = 86.3% R-sq (adj) = 84.5% This again confirms that the nett outturn doesnot depend on good leaf peercentage 4) The regression for refuse tea% X on Good leaf% RT = 12.4-0.124 GL (4) RT - refuse tea% GL - good leaf% Predictor coef st dev t-ratio Probability Constant 12.401 2.182 5.68 0.000 Goodleaf -0.12357 0.02949 - 4.19 0.001 *** s = 1.108 R - S Q = 52.3% R-SQ(ADJ) = 49.3% *** Highly significant - 49.3% of the variation of refuse tea% is explained by Good leaf % The results indicate that the Net outturn mainly depends on the moisture content of the green leaf, rather than the leaf standard. Using the above correlation (equation 1) if the moisture content in green leaf is known, the nett outturn could be predicted. It was also decided to collect samples of green leaf received at St. Joachim factory almost everyday for about one year and determine the moisture content to work out the net outturn achievable using the above equation and predict a possible average figure for the whole year. ANNUAL REPORT FOR 1997 185 TABLE - 07 Moisture Determination of green leaf samples received at St Joachim Factory Date Estate leaf Bought leaf MC% Surface moisture MC% Surface m 97/07/09 81.0 yes 79.9 yes 10 81.9 yes 82.5 yes 11 80.4 yes 81.4 yes 14 80.6 yes 78.7 yes 15 79.7 yes 79.1 yes 29 75.6 no 77.5 yes 30 75.6 no 79.6 yes 31 74.6 no 78.3 no 97/08/01 76.3 no 79.2 yes 04 75.8 no 77.8 yes 05 76.1 no 76.9 no 97/10/13 - - 78.9 yes 97/11/04 79.5 yes 79.3 yes 05 80.2 yes 81.4 yes 06 80.4 yes 80.9 yes 07 79.6 yes 79.2 yes 18 79.8 yes 80.1 yes 19 77.7 no 77.0 no 97/12/03 78.5 yes 80.6 yes 04 79.1 yes 80.6 yes 05 76.0 no 78.6 no 06 77.1 no 77.3 no 08 76.2 no 76.8 no 09 78.8 yes 79.6 yes 10 77.6 yes 77.3 yes 11 79.5 yes 80.3 yes 12 78.2 yes 77.6 yes 15 79.2 yes 80.3 yes 16 80.3 yes 80.1 yes 17 77.6 yes 77.5 yes 18 77.7 no 79.1 yes 19 78.9 yes 79.0 yes G.L.C. Galahitiyawa, and N. Vldanapathirana 6.5 Monitoring the standard of leaf at St. Coombs Factory Monitoring of the standard of leaf coming to St. Coombs factory was continued at least two days a week. The bought leaf coming from St. Coombs estate, Lamilliere and Diyanillakelle estates (bought leaf) were taken to monitor the standard of leaf brought to St. Coombs factory. 186 ANNUAL REPORT FOR 1997 Standard leaf Sub Standard leaf Bud itself Bud + three or more leaves Bud + one leaf and leaf with hard stem Bud + two leaves Coarse banji Bud + three leaves (if 3rd is tender) Coarse single leaves Tender single leaf Any damaged leaves Tender banji Several samples of leaf were taken after spreading on the troughs for each division separately. The respective samples were bulked and about 250 gm from each sample was taken to monitor the leaf standard. They were then divided into Standard leaf and Sub Standard, the percentages being shown below. The acceptable standard of leaf should be about 65% good leaf (minimum) for good manufacture. TABLE 8 - Standard of leaf brought to St. Coombs factory in three different places Months St. Coombs Lamillier Diyanillakele Count% Weight% Count % Weight% Count% Weight% January 53 48 55 49 58 54 February 62 55 63 58 57 54 March 57 49 58 55 60 53 April 63 53 55 45 57 48 may 49 42 58 48 51 40 June 53 54 61 51 65 55 July 59 45 62 51 64 46 August 58 43 63 51 59 50 September 65 52 61 51 70 59 October 61 63 68 55 69 59 November 59 42 61 48 69 46 December 58 46 57 46 65 52 S. H. Priyanthi, L. Jayasinghe 6.6 ISO 9000 ISO 9000 series certification has become and international standard for quality management. Many companies across the globe had obtained this certification and many more are in the process of obtaining the same. Although at present, the tea importing countries are not demanding ISO 9000 certification for the tea processed at a particular factory, by the turn of the century there is the possibility, it might become a prerequisite to have this certification for selling tea produced from each factory. ANNUAL REPORT FOR 1997 187 To achieve certification, a company must demonstrate its commitment to quality. To make it a success the total involvement of everyone in the company in the operation of the quality system is utmost important. There are three standards under this series. ISO 9001 design/development, production, Installation and customer service ISO 9002 production, Installation and customer service ISO 9003 final inspection and test What is relevant/appropriate to the industry at present is ISO 9002. The system requires documentation and demonstration. In other words, one must say what he does & do what he says. There are three manuals to be perpared, 1. Quality manual 2. Quality procedures manual 3. Work instructions With the intention of leading the way for other factories to follow, action was initiated first to obtain ISO 9002 certification for processing at St. Coombs factory. In this regard the assistance received from M/S Gihan Talgodapitiya Associates (Pvt) Ltd, Colombo 07, in preparing all three manuals is appreciated. The institute has appointed M/S Technoconsult (Pvt) Ltd., Colombo 06 as consultants to implement the system and obtain certification. As an initial step the consultants conducted several training programs on ISO 9000 for the benefit of the staff (Executive grade & Intermediate grade). It is anticipated to obtain ISO 9002 certification by mid 1998. M. T. Ziyad Mohamed, K. Raveendran 6.7 Packing Trials with New Type of Paper Sacks When the tea is packed in tea chest the cost of packing is the largest component in tea processing. As a result new packing materials are tested from time to time for their suitability for packing tea. In this study two types of tea bags (sacks), one with the inner ply having two joints of Aluminum/LDPE/Kraft paper and the other with matallized polyester/LDPE/Kraft paper were tested. The normal paper sack with the same dimension, but with inner ply having one joint was used as the control. The following information were recorded from each type of bag. 1. Sack dimensions 2. Grammage/ thickness 3. Tensile strength measured by the "drop test" 188 ANNUAL REPORT FOR 1997 52 kgs of BOP grade tea was packed in each sack (6 numbers from each type) and stored in the Division and the humidity in the room was also recorded. Initial moisture content of the tea was also recorded. At the the end of each month duplicate samples of teas were drawn from each bag and the moisture content and the chemical parameters such as TF, TR, total colour etc were analyzed. The samples were also evaluated by tasting (sensory evaluation) and also sent to three professional tasters for their evaluation. The results after 2 months of the trial are summarized below. Dimensions of Tea Sacks Empty sacks (lay fiat) dimensions Sack length(mm) Sack width (mm) Width of bottom(mm) Grammage (gsm) LDPF Aluminium Kraft Paper Control Safe tea sack 1078-1080 700 212-215 New tea bag New Metalized 20 20 65 New Tea bag Metalized 1100 540 272 % Colour New tea bag Al Kraft Paper 1080 520 315 Moisture New tea bag New tea bag Metalized Al Kraft Papeer 1055-65 703-705 200-204 1060 705 205-207 Filled sack dimension Sack length(mm) Sack width (mm) Width of bottom (mm) tea bag Al Kraft paper 20 21 65 Control Safe tea sack 1110 535 270 TF% TR9 Initial bulk Tea 0.8308 14.04 3.40 3.7 ANNUAL REPORT FOR 1997 189 After one month, Sacks TF% TR% Colour Moisture STS 1 0.9281 14.36 3.83 3.95 STS 2 0.8359 14.58 3.77 3.7 NTB(M) 1 0.8313 14.43 3.73 3.85 NTB(M) 2 0.7639 14.35 3.83 3.75 NTB(AL) 1 0.8224 15.17 3.94 3.6 NTB(A1)2 0.8314 14.76 3.99 3.8 After Two Months Sacks TF% TR% Colour Moisture STS 1 0.8392 14.35 4.00 3.0 STS 2 0.8100 15.45 3.89 3.7 STS 3 0.8932 13.78 4.00 3.8 STS 4 0.9070 14.10 3.74 3.7 NTB(M) 1 0.8452 14.36 3.90 3.9 NTB(M) 2 0.8674 14.77 4.02 3.8 NTB(M) 3 0.7800 14.85 3.97 3.9 NTB(M) 4 0. 8320 14.87 3.88 3.7 NTB(AL)1 0.8336 12.88 3.81 3.7 NTB(AL)2 0.8885 14.14 4.12 3.9 NTB(AL)3 0. 8460 14.59 3.39 3.9 NTB(AL)4 0. 8430 15.40 3.93 3.8 These trials are expected to continue till end of February next year. M. T. Ziyad Mohamed, K. Raveendran, S. H. Priyanthi, L. Jayasinghe 7. Seminars/ Lectures Dr. M. T. Ziyad Mohamed Presented a paper titled " Keeping quality of Teas" at a seminar organized by John Keels Ltd at TRI, Auditorium, Talawakeile on 3rd January. Presented a seminar titled "CTC Manufacture in Kenya" based on the information gathered during his visit to Kenya in October/November 1996, at TRI, Auditorium on 22nd January. Addressed RSC I seminar for Superintendents/ Asst. Superintendents at TRI, Auditorium on 27th February. Addressed RSC I seminar for Factory Officers at TRI, Auditorium on 10th March. 190 ANNUAL REPORT FOR 1997 Addressed RSC VII seminar at Hotel Reef Comber, Hikkaduwa on 14th March. Addressed a seminar organized by District Planters' Association at Talawakelle Club on 21st July. Attended a seminar and exhibition titled ' Tea and Health' in Colombo on 14th and 15th August. Delivered a lecture on ' Tea Manufacture' for the students following Plantations Evaluation Course at Faculty of Agriculture at University of Peradeniya on 06th September. Addressed the staff of Watawala Plantation Ltd on ' Finer points in tea Manufacture' at Waltrim Factory on 29th September. Addressed a seminar on ' Tea Manufacture' for Superintendents and Factory Officers at Maskeliya Club on 1st October. Addressed the participants on ' Finer points in tea manufacture' at Mid Country RSC IV seminar held at Hantana on 11th December. 8. Training Programs/Paper presentation Dr. M. T. Ziyad Mohamed served as a resource person for the following factory officer training programme (skill development in tea manufacture), at NTPM Bogawantalawa on the following dates; 27 th and 28th April 04 th 06th May 06 - 09th July 14 - 17th September 11th- 12th November 12 - 14th December Dr. M.T. Ziyad Mohamed conducted Factory Officer training programe on ' Tea Manufacture' for Factory officers attached to Kahawatte Plantations Ltd on 2nd May. Factory Officer training programe on ' Tea Manufacture' for Agrapathana Plantation Ltd on 19th May, at Agras Club. Training programe on ' Tea Manufacture' for Superintendents and Assistant Superintendents on 20th May at Agras Club. ANNUAL REPORT FOR 1997 191 Dr. M.T. Ziyad Mohamed served as a resource person for National Certificate Examination in Tea Manufacture for factory officers on 12th June, 17th July and 25th September. Dr. M.T. Ziyad Mohamed presented a paper on Solar Energy Project at a seminar held at NARESA on 29th October. Mr. G. C. Galahitiyawa served as a resourse person for factory officer training programes on " Tea Manufacture" on the following dates at NIPM Bogawantalawa. 17th, 19th and 20th July 28th, 30th and 31st August 15th September 25th, 27th and 28th September 10th November Training programes on " Tea Manufacture" for Assistant Superintendents at NIPM on 12th June. 9. Visitors The number of visitors during the above period to the Technology Division was 12. 10. Advisory Reports During the period under review, 29 advisory visits to factories were made by the members of the Technology Division, including low country, on various aspects of tea manufacture and factory development. This excludes seminars and NIPM factory officer training programmes. The number of samples received from estates for determination of moisture content percentage was 1009. These were reported with advice for correction of defects wherever necessary. STATISTICS T.S.N. Senaratne General Ms. N Senaratne continued to assist various research disciplines of the Institute in statistical analyses of their experimental data. She served as Assistant Coordinator for Information for Agricultural Research Managers (INFORM). She also completed Part I & Part U of the ACS (Australian Computer Society) examination. In 1997 the following standard analyses of experimental data were carried out, mainly using the SAS statistical program: Completely Randomised design 10 Randomized Complete Blocks 195 Split-Plot 64 Split-split-Plot 7 Incomplete Blocks 27 Of these, 21 had factorial treatment structure and 6 involved covariance analysis. Correlation/Regression analysis 15 On the adrninistrative side, a program was developed in Visual Basic for presentation of St Coombs workers' salaries. AGRICULTURAL ECONOMICS UNIT Officer -in -Charge J.A.A.M. Jayakody 1. General Mr. B. Sivaram left the TRI in October after completing a three and a half years consultancy period in the unit. Mr Dilkalum Pallihakkara, NDT trainee from the NAB, completed his 4-month training programme in March. Miss H.W. Shyamali started working as a temporary Research Officer w.e.f April, for the CADMAR research prioritization programme under the financial assistance of the GTZ- CARP research management project. Miss.M.H. Attanayake joined the unit in June as a graduate trainee of the Ministry of Youth Affairs. Mr R. Ravishankar, final year student from the Department of Extension, Faculty of Agriculture, Peradeniya completed a 3-month research programme in October on "Ergonomics of Tea Plucking". 2. Divisional Activities: 2.1. Research Prioritization. The Composite Approach to Decision Making in Agricultural Research (CADMAR) approach is continued and co-ordinated by the AEU with the technical assistance of the GTZ-CARP research Management project. In this approach 25 applied research thrusts with 82 projects covering most aspects of the problems/constraints of the tea sector were identified for prioritization. There are 8 steps in the process of implementation of CADMAR, namely (i) Identification of the problems and constraints limiting productivity and/or the profitability of the industry, (ii) Classification of them according to researchability, (iii) Formulation of research thrusts, (iv) Assesment of the profitability of success of and the time needed for completion of the Research Thrusts, (v) Estimation of the costs of the research Thrusts, (vi) Estimation of the Aditional Benefits of the Innovations resulting from the Thrusts, (vii) Tabulation and presentation of the data which permits the comparision of Research Thrusts, and the setting of Research Priority and finally (viii) Prioritization of the Research Thrusts. Step (vi) is now almost complete and the next two steps could be completed within the first quarter of the next year. In order to balance the current and future research information requirements of the Institute a basic research programme has also been identified including fifty two basic research projects. J.A.A.M. Jayakody and H.W. Shyamali 194 ANNUAL REPORT FOR 1997 2.2. Economic Studies The following studies were completed during the year 1997. 2.2.1. Financial Viability of Replanting in Estates and Smallholdings An analysis of financial returns of replanting was undertaken, separately for the estate and smallholder sectors based on the position prevailing at the end of year 1996. The results obtained are given in the table below. It shows the improved financial viability of replanting in all segments of the tea industry following recent market changes. NPV @ 15% interest rate Elevation Average NSA for Estates Smallholdings year 1996 (Rs/kg) Low 114.35 17608 211477 Medium ' 89.70 -4560 35796 Up 93.38 138151 54706 B. Sivaram and G. Ganewatte 2.2.2. Economics of Shade Management A cost-benefit analysis of shade establishment and management was done using replacement cost approach with the view of highlighting the importance of shade in tea. The following table summarises the overall annualised cost and benefits of shade management for one hectare of tea land. TABLE - Overall Cost and Benefits of Shade Management in Up and Mid Elevation(Rs/ha/year) Cost Quantifiable Establishment cost Shade Management Fertilizer Uptake Gain Total Non - Quantifiable Blister - Blight Occurence Rs Benefit 338.87 996.50 950.00 9002.63 11288.00 Addition of Organic Matter Fuel Wood Value Rs 7688.00 3600.00 11288.00 Pest and Disease Control Quality Improvement Less Soil Erosion Build Up of Soil Organic Matter Increased Water Holding Capacity B. Sivaram and G. Ganewatte ANNUAL REPORT FOR 1997 195 2.2.3. Domestic Resource Cost of Tea Production An analysis was done to examine the comparative advantage of tea production using the Domestic Resource Cost approach. B. Sivaram 2.2.4. Economic Evaluation of Bag Plants vis-a-vis Bed Plants An analysis was done to examine the economic significance of bag plants compared to bed plants using all quantifiable costs and benefits of the practices. Results showed a very small financial advantage in using bag plants compared to the bed plants. It is now important to convince tea producers of the the non quantifiable benefits that can be obtained by using bag plants compared to bed plants through the TRI extension programmes. G. Ganewatte and J.C.K. Rajasinghe 2.2.5. Financial Analysis of Replanting with and without Rehabilitation A preliminary analysis was done using data obtained from a tea field of the Waltrim Estate to compare the financial viability of two approaches to replanting old seedling tea fields. Direct replanting with SALT shows very high financial attractiveness compared to the conventional method. The result obtained is location specific and therefore it is not possible to reach a general conclusion at this stage. Since the long term impact of the SALT system is not properly assessed and the agronomic feasibility of the technique is not fully explored, the applicability of this result is very limited. G. Ganewatte 2.2.6 Optimum Pruning Cycle Length Preliminary results obtained from an analysis of field data from several estates in different locations indicated that more estates would need to be sampled, as results were very location specific. The object of this work was to determine the optimum pruning cycle length for tea fields in different locations. Data analysis is in progress for Concordia Estate, Nuwara Eliya. Further analysis is required to confirm the results. J.A.A.M. Jayakody , G. Ganewatte and M.H. Attanayake 2.2.7 Cost of Tea cultivation An analysis on the annual cost of tea cultivation in different locations was initiated with the objective of developing financial guidelines for decision making in tea cultivation. Cost estimation of establishing and mamatining a tea nursery, replanting and maintaining young and mature VP fields, maintenance of high and low yielding seedling fields in four different elevation categories were initiated. J.A.A.M. Jayakody and M.H. Attanayake 196 ANNUAL REPORT FOR 1997 2.3. Development Planning 2.3.1. Nuwara Eliya District Development Plan A complete report covering various aspects of estates and smallholdings of the district was prepared. B. Sivaram 2.4 Geographic Information System studies in Tea Sector 2.4.1. Development of a Tea Information System A collaborative project to study the potential use of GIS for Land Use Planning in tea estates continued between the Tea Research Institute and the Environment and Forest Conservation Division of the Mahaweli Development Authority. As it was decided to build up the GIS capabilities wiriiin the Institute by having basic software and hardware, PC Arc/Info and Arc View 3 software programs were purchased. An order has been placed to purchase a computer with the necessary technical specifications, in order to develop an Information System for the Plantation sector. A questionnaire was developed and is ready to be distributed to tea estates to collect estate information. J.A.A.M. Jayakody 2.4.2. GIS application for Tea Estate Plarming Results of the GIS study carried out in five tea estates in Nuwara Eliya District managed by the Kelani Valley Plantation Ltd, for the purpose of selecting suitable lands for forestry programmes were presented at the 3rd Annual Forestry Symposium held on 12th and 13th December 1997 organised by Department of Forestry and Environmental Science, University of Sri Jayawardenapura. J.A.A.M. Jayakody and R.M.S.S. Rajapakse 2.4.3 Land Use Planning for Development of Marginal Tea Lands A contract research project funded by CARP No 12/302/235 - "Land use planning for development of marginal tea land with multiple objectives in the mid-country" was completed. At the review of the project it was decided to include the soil information also in digital form. Therefore soil surveys were initiated in November 1996 in five out of the six estates where the land use study was carried out. The soil survey was completed in Westhall, Craig head, Galphelle and Hantane Estates, and is still in progress at Rattwatte estate. The soil surveys were carried out in collaboration with the SPND of TRI and ANNUAL REPORT FOR 1997 197 the ENDEV project of the Mahaweli Authority, Polgolla. Work at Westhall estate was carried out as a case study to train TRI staff in Aerial Photo Interpretation for identifiction of land forms for soil sampling and to describe soil profiles. J.A.A.M. Jayakody , R.M.S.S. Rajapakse and Manoj Jayawardena 2.4.4. GIS application for the smallholder sector A GIS application on the tea smallholder sector, to study the desirability of this technology being used in the sector, continued. The report presenting the final outcome is to be written jointly by the TRI, TSHDA and the EFCD of the Mahaweli Authority. J.A.A.M. Jayakody 2.5 Socio-economics Studies A survey was initiated to study the labour scarcity problem prevailing in the Low Country tea estates due to labour migration away from tea sector. The first questionnaire of a set of three was prepared and pre-tested in the Deniyaya area to collect information from the mamagement point of view. Development of the other two questionnaires to collect data from the workers' point of view is in progress. J.A.A.M. Jayakody, G. Ganewatte , J.C.K. Rajasinghe and J.A.S.K.V. Jayasinghe 2.6 Miscellaneous Activities Mrs. J.A.A.M. Jayakody and Gaminda Ganewatte continued to participate in the monthly group meeting of Socio-economist and Policy Analyst in the National Agricultural System, organised by the CARP from 21 August 1997. 3. Publications and Presentations 3.1. Nuwara Eliya District Development Plan A Monograph covering almost all aspects of development of estate and smallholdings in Nuwara Eliya District was published. 3.2. "Tea Bulletin" Two articles titled "Replanting Reassessed" and "Economics of Shade Management in Tea Estate" were submitted for publecations in the next "Tea Bulletin". 198 ANNUAL REPORT FOR 1997 3.3. TRI Update AEU contributed during the year by providing an article on "Bag Plants vis-a-vis Bed Plant - an Economics Overview". 3.4 "Ranpath" AEU contributed to the "Ranpath" Sinhala magazine published by TSHDA, providing an article on financial gains from replanting. 3.5 Other "Land Use Planning for Agro-Forestry Programmes in Tea Estates" was submitted to the proceedings of the third annual forestry symposium of the University of Sri Jayawardenapura. 3.6. Seminars and Meetings The following meetings and seminars were attended by the divisional staff: i) Presentation on "Economics Aspects of Nitrogen Fertiliser" by Mrs. J. A A.M. Jayakody at 194th E & E meeting on Revised Fertiliser Recommendation in June. ii) Presentation on "Worker motivation" at the seminar by Mr. Sivaram organised by PHSWT in Ratnapura in July. hi) Presentation on "Productivity and Quality Improvement in Tea" by Mr. Sivaram at a PMC seminar. iv) Presentation on "Land Use Planning for Agro-Forestry Programmes in Tea Estates" by Mrs. J.A.A.M. Jayakody at the 3rd Annual Forestry Symposium held on 12th and 13th December 1997 organised by Department of Forestry and Environmental Science, University of Sri Jayawardenapura. 4. Overseas Visit Mrs. J.A.A.M. Jayakody participated the Second Indian Annual ESRI/ ERDAS User Conference held in New Delhi on 2nd and 3rd December 1997. St. Coombs / Lamiliere Estate 1. General Mr. M. W. Newman Superintendent of Ouvakelle Estate was over looking this property upto 10th May Mr. Hiran Dunuwille was appointed as Superintendent on St. Coombs/St. Joachim Estates with effect from 10th May Mr. D. H.Wickremasooriya was appointed as Visiting Agent to St. Coombs and St. Joachim Estate from July 1997. Mr. P. Periyannen - Chief Clerk was transferred to St. Joachim Estate with effect from 01st June and Mr. A. R. William the Senior Assistant Clerk is acting for the Chief Clerk. Mrs. K. Asalinona who was appointed as Medical Assistant on casual basis retired in June. Mr. H. M. R. Kuladasa was appointed as Junior Assistant Factory Officer with effect from 1st June. Mr. S. Fernando was appointed as Estate Medical Assistant with effect from 23rd June. Mr. K. Selvaraj was appointed as driver with effect from 01st September. Mrs. K. G. S. Sriyani was appointed as Midwife with effect from 1st August. Mr. S. M. Sunil Shantha was transferred to St. Coombs Estate from St. Joachim Estate with effect from 01st December. 2. Weather and Rainfall: A rainfall of 2395.9 mm has been recorded over 199 wet days as against 2199.2 mm over 199 days in 1996. 3. Hectarage Statement The revenue extent of St. Coombs and Lamiliere were 98 Ha. and 48 Ha. respectively, totalling 146 Ha. 200 ANNUAL REPORT FOR 1997 4. Crop and Yield 1997 1996 Crop Yield Crop Yield St. Coombs Lamiliere 188,515 91,203 1,923 183,395 1,898 78,767 1,877 1,624 Total Bought Leaf Grand Total 279,718 104,319 384,037 1,915 262,162 113,432 1,915 375,594 1,793 1,793 5. Tea Prices Apart from topping the Western market on several occasion, St. Coombs recorded an all time highest price of Rs. 204/- per kilo for B.O.P. grade in March 97 and Rs. 176/- for Dust No. 1 in June. 6. Working Results Made a profit of Rs. 7,922,124.19 7. Nursery A total of 38,900 plants of clone TRI 2025 and from TRI 3000 and 4000 series were raised in the Nursery for planting and infilling. 8. Labour Labour on St. Coombs and Lamiliere has been quiet and the health of labour was satisfactory. Wiring (electricity) of labour cottages is in progress, A loan from TRI was granted for this purpose, and to be recovered from labour on installment basis. TABLE 1 Working account of St. Coombs/Lamiliere for the year 1997 compared to previous years. Year Total crop Bought Yield Nett sale kg(MT) leaf (Kg) (kg MT/ Average Ha) Rs. cts 1993 246,267 102,211 1696 87.02 1994 467,687 91,434 1,845 89.60 1995 292,530 107,765 2,001 93.52 1996 262,162 113,432 1,793 106.52 1997 279,718 104,319 1,915 120.30 MT - Made Tea Cost of Production Gross Actual Rs/kg Profit + Profit + Estimated Actual Loss - Loss - Rs Rs 74.36 88.43 -463,497.40 -463,497.40 84.36 85.01 1,828,191.13 1,828,191,13 83.03 82.56 2,965,830.76 2,965,830.76 83.09 92.69 5,194,465.00 • 5,194,465.00 85.79 99.49 7,922124.19 7,922,124.19 202 ANNUAL REPORT FOR 1997 Table 2 Monthly Yield (kg/ha), Rain fall and average 'N' applied from 1993 to 1997 - St. Coombs Estate Months 1993 1994 1995 1996 1997 January 109 172 206 148 161 February 57 153 168 102 86 March 90 140 136 74 58 April 178 199 184 93 69 May 125 199 276 424 413 June 236 146 172 105 140 July 119 112 119 150 203 August 92 105 190 140 115 September 155 163 132 93 109 October 146 173 205 164 190 November 192 164 184 198 196 December 196 170 178 186 193 Total 1695 1896 2150 1877 1923 Rain fall (mm) 2918.7 2356.1 2291.9 2199.2 2395.9 No. of wet days 220 220 188 210 199 Average N (kg ha-1 yr 1) 222 220 234 258 226 Table 3 Monthly yield (kg/ha) of fields with amounts of "N" applied - St. Coombs (1997) Field Ext­ Total Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec Total No. ent "N" Ha 1 7.1 200 64 75 85 471 181 189 166 126 172 200 181 2059 2 2.6 165 es 119 80 83 480 211 232 146 120 254 305 180 2276 3A 7 230 292 119 65 63 566 171 247 197 86 255 232 280 2573 3B 6.7 230 274 87 88 81 544 195 304 128 180 308 299 350 2838 4 9.1 200 '.78 118 66 83 481 212 298 155 140 239 231 186 2387 5 7.4 195 120 123 80 138 377 266 247 190 165 342 291 323 2662 6A 3 95 101 24 23 14 246 P 14 69 184 676 6B 2.9 95 112 42 31 37 113 P 53 16 404 7 4.7 230 149 142 44 68 520 136 203 125 158 183 203 130 2061 8 5.2 200 98 77 49 66 . 402 93 345 137 119 220 213 181 2000 9A 3 150 268 123 68 82 292 235 170 210 238 326 377 291 2680 9B 4.8 150 213 91 47 85 574 144 260 120 158 235 222 238 2387 10 0.5 165 594 246 176 88 334 324 346 108 206 138 192 272 3024 10B 2 165 159 73 77 49 320 176 274 122 145 249 297 198 2139 11A 2 230 266 125 73 179 428 110 157 80 188 442 221 343 2612 11B 4 190 128 98 56 48 264 129 156 76 63 99 136 223 1476 12A 1.2 230 272 144 84 71 564 186 222 128 130 115 163 261 2340 12B 7.1 90 94 57 50 29 301 71 173 75 48 108 112 93 1211 13 10.4 40 68 25 24 16 234 9 4 5 4 3 28 32 452 14 7.3 200 129 49 43 72 466 103 259 90 101 177 186 168 1843 98 161 86 58 69 413 140 203 115 109 190 196 193 1923 P - Pruned V.PTea Clones Last (Ha) Pruned 8. Cultural Operation Field No.l Seeding Tea (Ha) 1 Nil 7.1 2 Nil 2.6 3 0.4 13.3 4 Nil 9.1 5 Nil 7.4 6 Nil 5.9 7 Nil 4.7 8 Nil 5.2 TRI 777 Aug 2016-2023 1994 2025 & DN TRI 2143 Aug 2142-2025 1996 DT 1 & DT95 TRI 2027 July 2043,2025 1993 WT26 TRI 62/9 Oct 2025,3016 1995 DN.N2 CY9 TRI 2142 May 2023,2025 1996 TC9.DT95 & N2 TRI2025 June DN & N2 1997 TRI 2024 April 2025,62/9 1996 DT 1 & DT 95 TRI 2024, June 2025 & DT 1 1994 Planting Year 1953-1959 1993 Yield per Hect 1996 2791 Yield per Hect 1997 2059 Shade Dadaps & Grevillea 1964 1091 2276 -Do 1965-1966 2878 2705 Dadaps 1967-1968 Grevilliea & Calliandra 1862 2387 Dadaps & Grevillea 1970 999 2662 - D o - 1985-1986 1619 542 Grevillea 1962-1964 2213 2061 Dadaps & Grevillea 1962-1964 1932 2000 Grevillea Field No.l Seeding V.PTea Clones Tea (Ha) (Ha) 9 10 4.8 0.5 3 2 TRI3000 TRI 2025 SALT area Last Pruned Decern 1995 July 1992 11 12 13 14 4 7.1 9.1 1 2 1.2 1.3 6.3 TRI 2025 62/9, N2 TRI 2025 K 145 TRI 2025 7/27 & DN TRI 777, 2024 & N2 July 1993 June 1994 July 1997 June 1994 Planting Yield/Ha Yield/Ha Shade g Year 1996 1997 S 1935 1472 2500 Grevil'lea/ VP 1986 Dadaps S 1935 2730 2320 VP 1991 Grevillea/ 1993 Calliandra S 1935 1676 1857 Gravillea/ VP 1988 Dadaps S 1935 1128 1376 Gravillea/ VP 1985 Calliandra S 1935 1379 452 Do VP 1986 1961 2247 1843 Do ANNUAL REPORT FOR 1997 205 Table 4 Monthly Yield (kg ha 1), and average N applied from 1993 to 1997 - Lamiliere Devision Months 1993 1994 1995 1996 1997 January 105 167 184 120 149 February 86 129 161 98 76 March 111 149 114 63 58 April 202 143 171 153 76 May 174 172 196 287 311 June 189 134 146 113 186 July 108 107 60 118 196 August 79 112 134 123 111 September 158 192 93 82 142 October 125 115 161 133 191 November 185 158 141 176 209 December 176 161 144 158 193 1698 1737 1705 1624 1898 Average N kg ha-1 yr 1 222 220 217 215 213 Table 5 Monthly yield (kgha1) of fields and amounts of "N" applied - Lamiliere Division (1997) Field Ext­ Total Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec Total No. ent "N" 4 5.1 150 138 90 55 151 184 226 224 130 175 215 262 208 2058 4B 1.9 200 258 89 57 51 508 194 285 131 211 197 247 151 2379 5 1.5 80 52 61 41 27 168 87 81 43 55 72 59 51 797 6 2 40 55 24 18 13 201 76 101 51 77 63 89 62 830 6B 3 110 68 20 22 15 167 79 115 75 18 P 3 582 7 4.5 165 223 104 110 80 386 261 258 96 161 252 243 216 2390 8A 5 230 195 118 85 123 386 248 252 186 201 263 237 330 2624 8B 4 110 121 48 40 70 225 195 147 123 159 186 257 138 1709 9A 4 250 11 P 39 50 267 147 258 120 221 202 256 217 1788 9B 4 100 168 79 58 56 298 P 28 93 179 236 1195 10 6.6 200 194 97 56 69 388 240 213 126 134 224 239 197 2177 11 6.4 200 172 94 60 86 389 239 254 135 155 255 207 223 2269 48 213 149 76 58 76 311 186 196 111 142 191 209 193 1898 10. Report on Lamiliere Division 11. Cultural Operation to Field No. Seeding VP Tea Clones Last Planting Yield/Ha Yield/Ha Shade Tea Pruned Year 1996 1997 4A 5.1 TRI 2025 June 1984 988 2058 Dadaps 1996 Graville 4B 1.9 TRI 2025 May 1986 2241 2379 - D o - 1993 5 1.5 June 1935 689 797 - D o - 1993 6A 2 June 1935 767 1993 830 - D o - 6B 3 DT 1-WT26 August 1990 and 697 582 - and TRI clones 1997 1991 7 TRI 2025 July 1983 2118 2390 - D o - 1993 8A 5 TRI 2025 May 1987/1983 2055 2624 - D o - 1995 8B 4 TRI 2025 June 1989/1990 691 1709 - D o - DN N2 CY9 1996 W T 2 6 & 9A 4 TRI 2025 August 1979 1584 1788 - D o - 1996 9B 4 TRI 2025 May 1980 1861 1195 - D 0 - DN & CY 9 1997 10 6.6 DN and TRI 2025 May 1994 1967/1969 2248 2177 Dadaps 11 6.4 DN and TRI 2025 July 1995 1970/1971 2013 2269 Dadaps 3 ~1 ST. JOACHIM ESTATE 1. General Mr.S. G. Ekanayake functioned as Assistant Superintendent of St Joachim Estate and Mr.H. L.Dunuwila.Superintendent of St.Coombs Estate was appointed to overlook St Joachim. Mr.D.Hemanth Wickramasooriya of Kelani Valley Plantations Limited was appointed as Visiting Agent for StJoachim and he made his first visit to the property on the 6th September 1997.Estate Affairs Committee Meetings were conducted regularly with the ChairmanJDirector and other officials and matters perteixiing to StJoachim were fully discussed and remedial actions were taken.Messrs.Bartleet and Co., Ltd.,and Messrs.De Silva, Abeywardena & Peiris continued as Brokers for StJoachim during 1997.The brokers requested us to desist from using the word "Clonal" as concern was expressed by consuming countries since of late as the word "Clone" has become synonymous with genetically manipulated material.As such the selling mark "StJoachim Clonal" was changed as "New StJoachim" with effect from November,1997. This year too the factory functioned without the Head Factory Officer and a case is pending before the Labour Tribunal at Ratnapura.The Junior Assistant Factory Officer Mr.S.M. Sunil Shantha was transferred to StCoombs Estate with effect from 1st December, 1997.The Field Officer Mr.D J.W.Ranawake did not work on the estate during this year too as he was mobilised for Army Voluntary Service. 2. Hectarage as at 31st December,1997. ha Mature V.P. tea Land under rehabilitation Estate Nursery Timber Clearing Abandoned tea Crop Diversification-Coconut Land under Rubber Land under Paddy Intercropping -Tea/Rubber 54.07 27.75 1.50 3.34 0.65 0.93 12.30 8.74 7.59 116.87 Other lands Acquisition by Government, buildings,roads,ravines and jungle 25.11 Total extent 141.98 208 ANNUAL REPORT FOR 1997 3. Crop (made tea kg) The production on St Joachim Estate in 1997 compared to the previous year was as follows :- Year Esate crop Bought crop 1996 63,330 1,094,941 1997 66,847 996,106 The production on the estate showed an increase of 3,517 in comparison to the previous year.This represents an increase of 5.55%. 3.1 Bought Leaf The bought leaf manufactured at StJoachim Factory showed a decrease of 98,835kg in comparison to last year. This represents a decrease of 9%.This decrease is mainly attributed to few more private factories being opened in the district and very wet weather prevailed through out the year. 4. Prices All teas produced at StJoachim Factory were sold at the Colombo Auctions in the Main Low Grown catalogue.Messrs.Bartleet and Co. Ltd. and Messrs. De Silva,Abeywardena & Peiris sold our teas in equal proportions. The tea produced during the year was sold at a Nett Sale Average price of Rs.l25/71.The Nett Low Grown Average for the year was Rs.l29/47,thus the factory Nett Sale Average was Rs.3/76 below Low Grown Average.The average price paid for bought leaf during the year under review was Rs.19/ 29.5 per kg as against an average of Rs. 17/31.9 paid in the previous year.The working of StJoachim Estate resulted in a profit of Rs. 22,302,584/-. (Table 1) 5. Nursery The supply of planting materials to Small Holders in the district continued this year too.Sale of planting materials as compared to the previous year was as follows.(The distribution of plants was done through the Tea Small Holding Develp. Authority.) Year Shoots Income Plants Income supplied (Rs.) supplied (Rs.) 1996 1997 8,750 182,948 26,250 548,844 ANNUAL REPORT FOR 1997 209 TABLE 1 WORKING ACCOUNT OF ST. JOACHIM ESTATE FOR THE YEAR 1997 IN COMPARISON WITH PREVIOUS YEARS Year Total crop Yield/ha NettSale Estimated Actual +Profit sold(Made (Made Average tea kg) tea kg) (Rs/Kg) cost of Pro­ duction Rs. -Loss Rs. 1991 *726,162 75,190 1992 *338,205 31,463 1993 *690,355 55,775 1994 *865,691 57,487 1995 *887,732 55,643 1996 *1,094,941 63,330 1,162 55/96 693 70/00 1,014 74/70 1,045 69/49 1,097 78/89 1,248 1997 *996,106 1,236 66,847 113/81 129/90 65/17.16 60/09.21 -2,653,503/= 64/10.00 72/60.08 -2,999,959/= 71/65.59 72/37.69 +1,734,553/= 66/13.84 69/53.08 -35,914/= 68/80.25 75/88.3S +2,827,076/= 87/06.38 102/30.88 +17,978,620/= 98/24.30 :114/03 +22,302,584/= * Bought crop 1997 Production - 137,186 kg of made tea unsold as at 31/12/97 6. Cultural Operations Field No.l V.P.Tea Clones Last pruned Yield per hectare 1996 Yield per hectare 1997 Shade Experiments 5.85 ha T.R.I. 2023,2025,2026,2027 & S106 July,1996 372 kg 497 kg Albizia and Gliricidia Plucking experiment has been carried out by TRI in machine plucking and as well as manual plucking. Routine upkeep of the tea was done during the year. 1600 V.R plants of TRI 2025,2027 clones were used for infilling vacancies. 210 ANNUAL REPORT FOR 1997 Field No.lA 1.20 ha T.R.I. 2025,2027,S/106 & KEN May, 1995 2,124 kg 2,555 kg Albizia and Gliricidia nil Upkeep of mana grass was undertaken during the year. Routine upkeep of the tea was done Field No.2 V.Ptea Clones Yield per hectare 1996 Yield per hectare 1997 Shade 4.12 ha T.R.I. 2025,2026,2027 & S106 562 kg 790 kg Albizia and Gliricidia This field is under the supervision of the T.R.I. and is used for clonal proving trials. Field No.2A V.P.tea Clones Yield per hectare 1996 Yield per hectare 1997 Last pruned Shade . 0.93 ha T.R.I.2025,S106 2,988 kg 3,649 kg June 1995 Albizia and Gliricidia Intercropping area.Planted coconut in tea.Routine upkeep of tea and coconut was done during the year. Field No.2F V.P. tea Clones Last pruned Yield per hectare 1996 Yield per hectare 1997 Shade Experiments 6.78 ha T.R.I. 2025,2026,2027 & S106 May 1996 788 kg 1,641 kg Albizia and Gliricidia Machine plucking has been carried out by TRI Low Country Staff. Routine upkeep of the tea was done during the year V. P. tea Clones Last Pruned Yield per hectare 1996 Yield per hectare 1997 Shade Experiments ANNUAL REPORT FOR 1997 211 Field No3 V.P. tea Clones Last pruned Yield per hectare 1996 Yield per hectare 1997 Shade Experiments . 8.40 ha . T.R.I. 2023,2025 . July, 1997 . 1,452 kg . 788 kg . Albizia and Gliricidia .. Nil Routine upkeep of tea was undertaken during the year. Field No.3 Rubber Area The rubber in this area was tapped and the latex was given to Rubber Manufacturing and Exporting Corporation of Sri Lanka Ltd., at Kuruwita.Routine maintenance was carried out during the year. Field No.10 Rubber Area This field too was tapped and the latex was given to Rubber Manufacturing and Exporting Corporation of Sri Lanka Limited Kuruwita.Routine maintenance was carried out during the year. Field No.10 Rubber Area (Part) This was planted in 1990.The extent is 3.60 ha.Tapping was undertaken during this year.Latex was given to the Rubber Manufacturing and Exporting Corporation of Sri Lanka Ltd.,Kuruwita. Field No. 4 V.P. tea Clones Last pruned Yield per hectare 1996 Yield per hectare 1997 Shade Experiments .. 5.85 ha .. T.R.I. 2023,2025,2026,2027,H50/1,S106 .. June 1994 .. 1,030 kg .. 1,180 kg .. Albizia and Gliricidia .. Machine plucking has been carried out by TRI Low Country Staff. Routine upkeep was done during the year. 212 ANNUAL REPORT FOR 1997 Field No.5 V.P.tea ... 8.20 ha Clones ... T.R.I. 2023,2025,2027,H50/1 Last pruned ... May 1995 Yield per hectare 1996 . . .771 kg Yield per hectare 1997 . . .1,996 kg Shade ... Albizia and Gliricidia Experiments ... Weedicide and cover crop experiments were carried out Routine upkeep was done during the year.6475 v.p.plants of TRI 2025, 2027 were used for infilling vacancies. Field No.6 V.P.tea ... 1.50 ha Clones ... T.R.I.2025,2026 & 2027 Last pruned ... June 1995 Yield per hectare 1996 ... 2,943 kg Yield per hectare 1997 ... 3,121kg Shade ... Albizia and Gliricidia Experiments ... Nil Routine maintenance was undertaken during the year in tea area as well as the area under mana grass. Field N 0 . 8 B VJP.tea ... 4.30 ha This area which was excluded from tea-in-bearing hectarage in 1996 and was shown under abandoned tea.Now it is under mana grass. Field N 0 . 8 A V.P.tea ... 6.00 ha Clones ... T.R.I.2025,2026,2027,KEN,S106and 3063 Last pruned ... July 1997 Yield per hectare 1996 ... 2,396 kg Yield per hectare 1997 ... 1,782 kg Shade ... Albizia and Gliricidia Experiments ... Nil Routine upkeep was done during the year A N N U A L R E P O R T F O R 1997 213 Field N 0 . 8 C V.P. tea Clones Last pruned Yield per hectare 1996 Yield per hectare 1997 Shade Experiments . Albizia and Gliricidia . Nil .. 1.90 ha . T.R.I.2025,2027,KEN,S106 & 3063 . July,1997 . 2,540 kg . 1,538 kg Routine upkeep of tea was undertaken during the year. The following New Clearing work was undertaken during the year. Field N 0 . 8 D - 2.83 ha - Area under rehabilitation Upkeep of Mana,lopping and weeding were done during the year. Field No.l - 4.80 ha - Area under rehabilitation Upkeep of Mana,lopping and weeding were done during the year. Field N 0 . 6 - 6.08 ha - Area under rehabilitation Upkeep of Manajopping and weeding were done during the year. Field No.5 • 5.60 ha - Area under rehabilitation Upkeep of Mana,lopping,and weeding were done during the year. Field No.4 - 4.14 ha - Area under rehabilitation Upkeep of Mana,lopping and weeding were done during the year Field N 0 . 8 C - 4.30 ha - Area under rehabilitation Upkeep of Mana,lopping and weeding were done during the year Field N 0 . 8 B - 2.02 ha Replanting was undertaken in this field using Clones TRI 2025 2027 and 3061, 31150. The routine upkeep after the planting was done during the year V.P. plants were used. 7. Factory Routine upkeep of factory building and machinery was done during the year.Painting the factory roof and doors and windows was done.A 300 KVA new genarator was installed in the factory since the existing 200 KVA genarator was inadequate to supply electricity to the factory as well as to all the bungalows.Re-siting of machinery has to be undertaken because of the installation of a new drier. Tarring the factory road was undertaken. 214 ANNUAL REPORT FOR 1997 8. Buildings All buildings on the estate were well maintained during the year. Renovation of 1 set of cottage type building near V.R nursery to provide accommodation to 2 Jnr.Asst.Factory officers was undertaken 9. Labour Health condition of the population was satisfactory.There were no strikes or major disputes.The estate was unaffected by any form of violance or disturbances during the year. The monthly yield of StJoachim Estate for 1997 in comparison with the yields obtained from 1992 to 1996 is given in Table 2 while the monthly yield of each field is given in Table 3. TABLE 2 Monthly yield (kg ha-l),rainfall and average of N Applied from 1992 to 1997 - StJoachim Estate. Month 1992 1993 1994 1995 1996 1997 January 89 71 102 94 82 112 February 24 34 94 68 86 42 March 05 52 99 77 83 61 April 12 90 109 96 90 118 May 60 82 66 97 96 111 June 44 96 88 99 75 147 July 61 108 66 102 119 113 August 82 79 79 101 112 95 September 68 88 75 101 108 99 October 70 98 87 89 125 90 November 85 106 89 104 126 122 December 93 110 91 69 146 126 693 1014 1045 1097 1248 1231 Total rainfall (mm) 3993.7 4589.3 3679.0 4216.2 3655.0 4552.3 No.of wet days 185 226 217 208 188 199 Average N (kg-1 ha-1 yr-1) 160 154 133 165 119 143 Monthly yieldfKg ha-1) of fields with fertilizer mixture used and amounts of N applied of St Joachim Estate M O N T H S Fid Exte. Total Fertilizer J F M A M J J A S O N D No (ha) Mixture used 1 5.85 160 U/235 24 07 24 37 31 44 39 51 61 45 64 70 497 1A 1.20 160 U/235 219 104 175 309 178 222 215 188 238 222 233 252 2555 2 4.12 TRI EXP.BLOCK. 48 17 33 64 78 82 83 63 81 77 83 81 790 2A 0.93 160 U/235 125 52 79 228 254 371 459 348 351 468 435 478 3648 2F 6.78 160 U/235 128 76 63 144 139 144 133 138 178 156 166 176 1641 3 8.40 140 U/235.U/300 105 27 42 106 118 127 53 06 26 11 84 83 788 4 5.85 140 U/235.U/300 96 23 46 84 95 120 107 118 127 112 134 118 1180 5 6.80 160 U/235 75 35 44 66 93 94 103 104 110 97 117 84 1022 5J 1.40 160 U/235 63 11 32 25 89 144 79 96 75 130 102 128 974 6 1.50 160 U/235 248 147 210 210 154 338 290 226 310 265 379 344 3121 8A 6.00 160 U/235,U/300 231 60 105 266 201 296 171 170 21 36 88 137 1782 8C 1.90 160 U/235 221 65 99 160 130 277 202 - 35 22 144 183 1538 3A 3.34 160 T/750 73 41 68 73 38 106 67 51 101 90 88 97 893 54.07 112 42 61 118 111 147 113 95 99 90 122 126 1236 Advisory and Extension Services Division Actg. HeadS. Wimaladharma General: Mr. S. Wimaladharma was appointed as Actg. Head of the Advisory & Extension Services Division with effect from 20 t h January 1997. Mr. B.A.D. Samansiri, Advisory officer returned to the island on 8* December 1997 to begin the local research component of his M.Sc. programme. Mr. J.C.K. Rajasinghe, Advisory officer was transferred to the Mid Country Station to cover up the functions of Mid Country Advisory Officer who was on a study tour in India- Mr. M.K.S.L.D. Amarathunga Extension Officer has completed his post graduate M.Sc. programme part 1 on 21 November and commenced his research project on "Impact of variation of the weather parameters on crop environment and productivity of tea land in Sri Lanka." Mr. M.K.S.L.D.Amarathunga was transferred back to Ratnapura after covering up the duties of OIC Deniyaya who was on study leave for a period of nine months Mr. H. J.M. De Silva was transferred back to Hantana in July from Low Country Station after covering up the duties of Mr. M.K.S.L.D.Amarathunga . Mr. J. A. S. K.V. Jayasinghe returned to Deniyaya Station on 1 s t July after completing his post graduate Dioploma Course at Kothari, India. Mr. S.T. Yatawatte followed a Course on Plantation Management at Kothari Agriculture Management Centre, Coonoor, Tamilnadu State, India from 10* July to 31 s t September. The activities of Advisory and Extension service centres of Ratnapura, Passara, Talawakelle, Hantana, Kottawa, Deniyaya are as follows. Special Events 1. Tea Research Board has already identified low country as the area that contributes most to the total production of tea in the country. Consequently it has decided to establish a Central Advisory Service at TRI Ratnapura to enhance the Advisory and Extension service in the area with effect from 20 t h January. ANNUAL REPORT FOR 1997 217 2. Experiment and Extension Forum for the Small Holding Sector. An E & E Forum for the Small Holding Sector was held for the first time in Sinhala medium at TRI Low Country Station, Ratnapura on 5 t h December 1997. Activities conducted by the Advisory and Extension Division, Low Country Station Ratnapura S. Wimaladharma Senior Advisory Officer / Actg. Head 1. Advisory Correspondences 626 Advisory correspondences have been sent on routine advisory matters, preparation of development plans and on statistical information. 250 on call requests pertaining to the advisory matters were received over the phone and necessary advices given. 2. Advisory & Extension Visits a. Advisory Visits 45 Advisory Visits to the Company Estates and 39 visits to the smallholding / private sector were made by the Advisory Staff on the following subject areas. 1. Yield declining studies and productivity assessment. 2. Preparation of pruning programmes. 3. Monitoring of shade trees and wind belts. 4. Preparation of manuring programmes. 5. Problems related to the nursery failures. b. Extension Visits Extension visits were made to estate and small holding sector to; evaluate and monitor the TRI 3000 & 4000 series colonal blocks; carry out a preliminary survey on bag plants Vs bed plants; and inspect and monitor the fertilizer trials. 3. Visitors to the Station 432 visitors of whom 103 Estate Management Personnel, 120 small and 60 private estate holders and 79 others visited the station seeking advise in relation to the problems such as nursery failure, Canker and wood rot drought casualties, termite and shot hole damage, labour shortage, productivity decline of the lands etc. 70 Universitys Diploma students and 70 others also visited the station for educational purposes. 218 ANNUAL REPORT FOR 1997 4. Advisory & Extension programmes conducted The following Advisory and Extension programmes were conducted by the Advisory Staff in collaboration with research staff. a. Advisory Officers Meeting Advisory Officers Forum meeting was held on October 03, at the Center, which was presided by the Director TRI Advisory and Extension Officers and Officers in charge of TRI stations attended. b. E & E and RSC Meetings 1. An E & E Forum was held for the first time in Sinhala medium at TRI Low Country Station, Ratnapura on 5* December 1997 for the Small Holding Sector. 2. Senior Advisory Officer attended three RSC meetings conducted in Galle, Talawakelle and Kandy and addressed the executive staff of Plantation companies on Nursery Management and Pruning Practices. 3. Five Field days/seminar and four NIPM programmes for estate sector and three programmes for Small holder sector were held on pruning practices, fertilizer recommendation, harvesting and shear plucking pest and disease management, weed management and mitigation of drought hazards. 4. Eleven group/panel discussions for estate sector and twelve for small holding sector were held to exchange knowledge and experience on cultural practices and to draw up manuring and pruning programmes and development plans for their tea lands. 5. Ten skill development training programmes for the field staff of the estate sector and three for the small holding sector were held to develop practical know-how on nursery management and pruning techniques.Csee plate -1) Plate -1. Skill development nursery teaming programme conducted for small holders at Waralla Estate. Plate-2.Field demonstration on correct mixing of straight fertilizer at a small holding site in Balangoda. 6. Eight field demonstrations were conducted at the adaptive fertilizer trials sites at Deraniyagala, Kalawana, Balangoda, Palawatta, Horana and Ingiriya, to disseminate knowledge on correct mixing and application of straight fertilizers, and on the precautionary measures to be adopted to mitigate drought effect (see plate No. 2). ANNUAL REPORT FOR 1997 219 A new adaptive fertilizer trial was established in Horana to conduct the community participatory agriculture demonstration for small holders in collaboration with Soil and Plant Nutrition Division / TRI and TSHDA - Regional centre - Kalutara. 7. Twenty three training programmes were conducted for University Students, Diploma students and G.C.E(0/L &A/L) Students in tea growing areas on " Advantages of the adaptation of eco-friendly economically viable agriculture practices for sustainable productivity of tea land" 8. A meeting was held with TSHDA officials to discuss the programme of work of TRI/TSHDA adaptive trial in Kegalle, Kalutara and Ratnapura areas. 9. "Mass Media" approaches to disseminate knowledge. a. Lak - handa Radio programme Senior Advisory Officer attended the radio programme conducted live by Lak - handa Radio unit on the industry and its benificial effects. b. Video Programme Preliminary work on production of a documentary video programme titled 'Tea Industry in Sabaragamuwa Province" commenced in December to telecast at the 50th Anniversary Independence Exhibition to be held at the BMICHin 1998 (S.L.D.Amarathunga). c. Documentary Report The documentary report titled "Significance of the TRI Low Country Station on the tea industry in Sabaragamuwa province" was prepared by the Advisory Division and submitted to the Sabaragamuwa Province Exhibition Committee to be included in the documentary report "50 years progress in Sabaragamuwa Province," for the 50* Anniversary Independence Exhibition to be held at the BMICH in 1998 (S.L.D.Amarathunga). d. Participation at exhibitions 1. Sri Lanka Tea Exhibition and International seminar - at BMICH, 13-16* August, 1997. 2. Mahapola Exhibition at Baddegama. 3. 53 r d Annual session - of SLAAS at University of Ruhuna from 8 to 12 December,1997. 4. Preparation for the 50 t h Anniversary Exhibition at BMICH commenced. 9. Research and Development a. A detailed survey commenced to collect information on the regionale profile on the extent, owner-ship pattern & distribution, productivity, weather pattern, soil status, manuring & pruning programme, factory information to prepare computer data base on Low Country Tea Planting District. (SW/SLD) 220 ANNUAL REPORT FOR 1997 b. A nursery survey commenced to collect information on the performance of the bag plant vis-a-vis bed plant (SW) c. Socio economic information was collected from the Community Participatory Training Programme and the demonstrations conducted at six sites where fertilizer adaptive trials are carried out. (SLD) d. Updated the display panel at the Museum of Low Country Advisory Division. (SLD) e. Preliminary arrangements have been made to prepare the model of a tea garden to display the cultural practices to be adapted to mitigate adverse effect of El - Nino at 50 t h Anniversary Exhibition at BMICH. (SW/SLD) 10. Soil pH analysis 569 Soil Samples were tested for soil pH and nursery suitability. 11. Advisory Publications Distributed 1. Advisory Leaflets (issued free) 1020 2. Publications Sold 32 12. Special Problems Tea growers were advised on the precautionary measures to be implemented to mitigate adverse effect of the consequences of El - Nino which is expected in early part of 1998 by conducting informal discussions, field visits, demonstrations and distribution of leaflets and other publications. Activities conducted by the Advisory and Extension Division, TRI, Talawakelle. Officer in Charge C.C.Rajasingham 1. Advisory Correspondences 515 Advisory correspondences were sent on routine Advisory matters. 2. Advisory & Extension Visits a. Advisory Visits 105 Advisory visits to the Company Estates and 21 visits to the small holding sector were made by the Advisory Staff. 3. Visitors to the Station 950 visitors including Estate Management Personnel, small and private estate holders and 2249 University Students / Diploma students and other students visited the station. ANNUAL REPORT FOR 1997 221 4. Advisory and Extension Programes The following Advisory and Extension programmes were conducted by the Advisory Staff in collaboration with research staff. 1 RSC Seminar The following four RSC seminars were organized by the Nuwara Eliya District Planters Association (NEDPA) at TRI Talawakeile. a. Seminar on "Tea Manufacture" for Superintendents and Asst. Superintendents. b. Seminar on "Qulity Tea Manufacture for Western Quality season" for the Factory Officers on 10 t h March. c. Seminar for Superintendents and Asst. Superintendents on 29 t h The following subject matters were discussed and "Management of Blister Blight" "Tea Nursery Technology", and "Harvesting practices to obtain better prices" 85 planters and 25 TRI officers participated. d. Seminar for Superintendents and Asst. Superintendents was held on 4 t h September on the subject of "Integrated Soil Management", and "Proposal for Development Plan for Nuwara Eliya District" 87 Superintendents, 19 Asst. Superintendents and TRI officers participated. e. Four group discussions were conducted for Asst. Managers and Field Officers of estates managed by Maskeliya, Bogawantalawa and Madulsima Plantation Companies at the TRI. The subjects covered were "Dry weather pests", "SALT and cover crops in tea" and "Effects of drought in tea field". 13 participants attended the discussion. Short lectures were conducted by senior scientists on relevent subjects which were followed by open discussions. 2. Workshops on plucking and interrelated activities were conducted for field staff and workers attached to 13 estates managed by 4 plantation companies and some private estates. A total of 520 field staff and workers participated in these workshops which were held at TRI Talawakeile. In addition, another workshop was conducted on "Nursery Techniques" to ensure success in the nursery and identify common error in the nursery. 3. Seven nursery training programmes were conducted for the following estates: Concordia, Annfield, Ferham, Edinburgh, Ingestre, Pedro, Glassaugh and Diyagama. 4. Seventy one plucking seminars were conducted for estate field staff, Kanganies and pluckers. 222 ANNUAL REPORT FOR 1997 5. Extension programme for small holder sector Three workshops on nursery practices, two seminars on plucking and replanting were held for small holders in the Nuwara Eliya District. 6. Six lectures and demonstrations were conducted for under graduate students of Affiliated Universities and diploma students of Aquinas, Gampaha Polytechnic College, Puliyankulama and Kundasale Technical Colleges. 5. Preparation of Extension Teaching Materials a. Video Programme Video filjaiing of earthworm project at Kotmale and on shear plucking Craig Estate Bandarawela (J.C.K.Rajasinghe) at completed Preparation of video film on "Replanting" is nearing completion (J.C.K.Rajasinghe and Bandula Perera) b. Advisory booklet on "Chemical control of tea peste" has been prepared (V.S.Sidhakran). c. Preparation of 150 slides in various field operations has been completed (V.S.Sidhakaran). d. Preparation of an advisory leaflet on "Field planting of tea" is in progress (J.C.K.Rajasinghe with staff from Agronomy Division). 6. Research and Development Information on general profile of upcountry tea lands was collected and processed to formulate a development plan for tea in the Nuwara Eliya District. This information includes the general distribution of tea lands and their productivity and resilient agricultural problems faced by the estate and small holders and the recommendations to improve the agricultural aspects in the district. This has since been published by TRI in December 1997 (C.C.Rajasingham and V.S.Sidhakran). A preliminary study on possibility of extending length of pruning cycle was initiated by gathering information from estates in Upcot area(J.C.K.Rajasinghe and V.S.Sidhakran). Publication on 'Tea nursery survey" conducted in the up country estates in 1995 has been updated and forwarded for publication. (V.S. Sidhakaran, C.C. Rajasingham) Exhibitions participated: Staff attended the following exhibitions Agricultural, Animal husbandary and Environmental exhibition at Nuwara Eliya. Mahapola Exhibition at Baddegama (with TRI Kottawa) and Kuliyapitiya (with TRI-Kandy) and Tea & Health Exhibition at BMICH-(with TRI- Ratnapura). Industrial exhibition at Ruhunu University in collaboration with TRI Ratnapura. ANNUAL REPORT FOR 1997 223 Activities conducted by the Advisory And Extension Division, Mid Country, Station Kandy. Advisory Officer : S.T. Yatawatta. 1. Advisory Correspondences 295 Advisory correspondences were sent on routine Advisory matters. 2. Advisory & Extension Visits a. Advisory Visits 101 Advisory visits to the Company Estates and the small holding sector were made by the Advisory Staff. 3. Visitors to the Station 557 visitors including Estate Management Personnel, small and private estate holders and 111 University Students, Diploma students and other students have visited the station seeking advise on tea cultivation and related problems. 4. Advisory and Extension Programes conducted 1. RSC Seminar Five RSC seminars were held for the Superitendents and Assst. Superintendents. Subjects covered were pruning, nursery management and drought management 2. Ten Field day sand seminars were conducted for field staff of estate to impart practical know-how on areas such as drought management, nursery management and other cultural practices. 3. A tea manufacturing seminar was held for the Factory Officers. 4. A seminar was held to disseminate recent research findings to the Tea Inspectors and Extension Officers. 5. Ten Field days/Seminars were conducted for the tea small holding sector. 6. Four practical training programmes were conducted for the Diploma holders of Anuradapura, Akmeemana, Kundasale and Vavunia Technical Colleges. 7. Senior Advisory Officer conducted lectures and demonstrations for field officers at NTPM, Bogawanthalawa on 23 November. 8. A meeting was held with TSHDA officials to discuss the co-ordination of TRI/TSHDA adaptive trial in Kandy. 9. Exhibition participated Staff participated at Mahapola Exhibition held at Kuliyapitiya from 5 t h Jan. to 04 t h Feb. and at Sri Sumangala Vidyalaya, Kandy. 224 ANNUAL REPORT FOR 1997 10. Soil sample analysis 1629- Soil samples were anlysed for pH value. 11. Research and Development activities. a. Advisory Officer attended to the Sinhala translation of the TRI Field Guide book b. Updated the regional statistical information on the tea growing areas in Kandy. Activities conducted by the Uva Advisory And Extension Centre, Passara Officer in Charge : M.B.A.Perera 1. Advisory Correspondences 294 Advisory correspondences were sent on routine Advisory matters and other problems. 2. Advisory & Extension Visits 74 Advisory visits to the Company Estates and the small holding sector were made by the Advisory Officer. 3. Visitors to the Station 304 visitors including Estate Management Personnel small and private estate holdings, University students, Diploma students and other students visited the station. 4. Advisory and Extension programmes conducted 1. Advisory Officers Forum Advisory Officers Forum meeting was held on February 24 t h at the Center. Dr. G.D. Wimaladasa Deputy Director Research (Production) also participated with the Advisory and Extension Officers and Officers in charge of TRI regional centres. 2. RSC Meetings a. Two Regional Scientific Committee meetings were held at TRI Uva Centre on 9 t h January and 17 th March. b. Advisory Officer in charge attended the RSC Kandy seminar held at Gannoruwa, on 5* March and Southern RSC seminar held at Hikkaduwa on 14* March. c. RSC- seminar was held at Bandarawela on 13* June on the subject "Productivity improvement in Uva tea lands". d. RSC group discussion was held at Uva Centre on 24* June for planters from Passara, Lunugala, Madulsima and Namunukula sub regions. ANNUAL REPORT FOR 1997 225 e. Three Group discussions were held at Hali Ela and Bandarawela for Asst. Superintendent of Agarapatana Plantation Ltd. 3. A field day was held on "Shear Plucking" at St. James Estate, Hali Ela for the staff of Malwatta Valley Plantation Ltd. on 10th January. 4. Special field day was held at Malwatta Valley to study extent affected by the weed Pasali by a team of scientists from TRI and University of Pearadeniya on 25* January. 5. Advisory Officer in charge participated at a seminar on "Pesticide Safety" at TRI Talawakelle on 22 January. 6. Ten Seminars for small holders were held at Medawelagama, Attampitiya, Welgolla, Welimada, Lunugala, Kandahena, Bandarawela, Agratenna and Boralanda. 7. Two field days were held at Verellapatana and Conogolla estate on dry weather pests. 8. Advisory Officer in charge served as a resource person for two training programme conducted by NIPM, for field officers. 9. Advisory Officer in charge participated as a resource person for training programme conducted by TRI Kandy, for field officers. 10. A meeting was held with TSHDA officials to discuss the co-ordination of TRI/ TSHDA adaptive trial in Uva. 11. Exhibition participated a. Sri Lanka Tea Exhibition and international seminar at BMICH, 13 -16 t h August b. Mahapola Exhibition at Baddegama in September. c. 50* Anniversary Exhibition at BMICH. 5. Research and Development a. Out door filming to produce a video film on 'Land selection and preparation of land for Replanting tea" is nearing completion (J.K.C.Rajasinghe and M.B.A.Perera). b. Advisory Officer attended to the Sinhala translation of the TRI Field Guide book. c. Preparation of a leaflet on 'Tea Industry in Sri lanka" for the 50* Anniversary of Independence Exhibition(M.B.A.Perera). 226 ANNUAL REPORT FOR 1997 6. Soil analysis 1629- Soil samples were anlysed for pH value. 400- Soil samples were tested for Organic Carbon %. 7. Special Problem encountered Terminal die back on Clone TRI 2023 and poor recovery after pruning was observed in some estates in Uva region. Variegated foliage and toxicity symptoms were also observed in young tea fields and field recovering from pruning. Activities conducted by the Advisory and Extension Centre, Kottawa Advisory Officer / OIC: K. D. Dahanayake 1. Advisory Correspondences 114 Advisory correspondences were sent on routine Advisory matters and other problems. 2. Advisory & Extension Visits 81 Advisory visits to the Company Estates and the small holding sector were made by the Advisory Officer. 3. Visitors to the Station 171 visitors including Estate Management Personnel, small and private • estate holdings University and Diploma students and other students visited the station. 4. Advisory and Extension programmes conducted 1. Advisory Officers Forum Advisory Officers Forum meeting was held on 26 May at the Center. DDR(T) participated with the Advisory and Extension Officers and Officers in charge of TRI regional centres. 2. RSC - Seminars Organised two seminars for estate executives and officers from TSHDA and Tea Commissioner's Division. The Director, Actg. Head Advisory and Extension Services, Chairman, Vice Chairman and General Secretary of Planter's Association also participated. The subjects discussed were Plucking, Pruning, Fertilizer, Weed management, Nursery and Pest and Diseases. 3. Seminars for the estate executive staff 20 seminars were held to estate executive staff attached to Watawala, Namunukula, Elpitiya and Hayleys plantations Ltd. and John Keels on Plucking, Pruning, Fertilizer, Weed management, Nursery, Shear plucking, Shade management and Pest and Diseases. ANNUAL REPORT FOR 1997 227 4. Training Programme for Small Holding Sector 59 Groups of small holders were trained at Kottawa Centre and also at Galle, Matara, Baddegama, Elpitiya, Hiniduma and Udugama areas. The topics covered were on Plucking, Pruning, Fertilizer use, Chemical weeding, Nursery and Shade. The usual problems encountered by them were raised and satisfactory solutions given. 5. Fifteen small holder group discussions were conducted at the Centre on the following topics: Cultural operations, shear harvesting, Fertilizer application, Dolomite application and pH testing. 6. Four other training programmes for officials of Government Department, Fertilizer companies, Teachers and School children on all cultural operations and effects of El - Nino were conducted. 7. Four "Sramabimana" Training Programmes were conducted for rural youth pluckers including small holders of the area with the assistance of TSHDA officials at Kottawa Centre. 8. Officer in Charge participated at District Co- ordination Committee meeting held at Town Hall, which was presided by the Hon. Minister of Education and Higher Education. 9. Advisory officer assisted the monitoring of adaptive research trials (TRI/ TSHDA) at Akuressa, Hiniduma & Elpitiya. 10. Exhibition attended Staff participated at Mahapola Exhibition at Wanduramba Central College, in September. 11. "Tea Shakthi" programme Officer in Charge participated at the 'Tea Shakthi" programme conducted by TSHDA at Deniyaya, Kananke and Wandumraba. Activities conducted by the Deniyaya Advisory and Extension Centre, Diyadawa Extension Officer/Actg. OIC: J. A. S. K. VJayasinghe 1. Advisory Correspondences 310 Advisory correspondences were sent on routine Advisory matters and other problems. 2. Advisory & Extension Visits Advisory Visits, 34 Advisory Visits to the Company Estates and the small holding sector were made by the Advisory staff. Extension Visits 51 inspection visits were made to experimental plots & sites where adaptive fertilizer trials are on. 228 ANNUAL REPORT FOR 1997 3. Visitors to the Station 578 visitors mcluding Estate Management Personnel from small and private estate holdings University and Diploma students and other students visited the station. 4. Advisory and Extension Programmes Conducted Advisory Officers forum Officer in charge attended the Advisory Officers' forum meetings held at Passara, Kottawa and Ratnapura. 1. RSC - Seminar Regional Scientific Committee (VII) organised two seminars in collaboration with TRI Kottawa for estate executives and officers of TSHDA and Tea Commissioner's Division. The Director, Actg. Head Advisory and Extension Services, Chairman, Vice Chairman and General Secretary of PA attended. The subjects discussed were Plucking, Pruning, Fertilizer, Weed management, Nursery and Pest and Diseases. 2. Two field days were held for small holders on Nursery management, Pruning, & Infilling. 3. Five programmes were conducted for estate executive staff, one on Nursery management and four on all agricultural practices. Two student programmes on all agricultural practices. One worker programme on plucking. Two programmes for University students on all agricultural practices. 4. Seminars Ten seminars were held for small holders covering all agricultural practices Two seminars were held on nursery practices. One panel discussion was held on pruning. 5. Demonstration 2 field demonstrations were held for small holders and estate staff on pruning. 6. Informal discussions Seven programmes were conducted for estate staff on agricultural practices, pruning and machine harvesting. Three programmes were conducted on Plucking, Nusery management, Soil sampling and Agricultural practices. Three programmes were conducted for Tea Inspectors on agricultural practices and adaptive extension trials. ANNUAL REPORT FOR 1997 229 7. Video progTammes 35 Video films were shown to planters, small holders and students on Plucking, Nursery management, Soil fertility and Mites. 8. Field Trials a). Shear plucking Vs manual harvesting A trial commenced at this station to study the effect of different methods of plucking (TRI shear, Indian shear & hand plucking) on yield of tea, in collaboration with Agronomy division, b). The staff attached to the station also helped the research division on the following projects carried out in the region: 1. Effect of earth worm on yield of tea (Anninkanda Estate) 2. The effect of application of Potassium and / or Magnesium from Sul Po- Mag and Kieserite at 2 levels of N on Soil / Plant nutrient status and yield of tea (Kiruwanganga Estate). This experiment is in progress. 3. Me Br- 06 To test alternatives to MeBr to eradicate Eelworm in tea fields(Handford Estate). This experiment is in progress. 4. Experiments to investigate effective time and type of pruning (Kiruwanganga Estate). This experiment is in progress. 5. Effective combination of shade / fuel wood tress for diversification (Kiruwanganga Estate). This experiment is in progress. 6. Adaptive fertilizer trials at Pasgoda & Deniyaya were conducted by the TRI in collaboration with TSHDA Matara region. 7. LVP - 43 Siduwa Estate plucking continued from January 1997 and yield records were maintained. The summary of activities carried out by the Advisory & Extension Service of TRI Subject Ratnapura Deniyaya Talawakelle Passara Han tana Talgampola Adv. Correspondence 626 310 515 294 295 114 Adv. Visit 137 34 105 74 101 81 pH Tests 569 87 - 939 1629 171 Visitors 432 578 3199 304 - 742 Training Prog 28 33 7 19 10 104 RSC Meetings 4 2 - - 5 6 NIPMProg 4 - - - 2 - Group Discussion 38 17 10 - 15 71 TRI/TDHDA 4 3 - - 1 - Video Prog. 2 35 - - - - Exhibition 4 - 5 - 2 - Org. C % Tesrts - - - 400 - - Free Leaflets 1020 - - - - - Priced Pub. 32 - - - - - LOW COUNTRY STATION, RATNAPURA. Officer-in-Charge - Sushila I. Vitarana 1.0 General S. I. Vitarana assumed duties as the Officer'-in-Charge of the station with effect from 20th February in addition to her normal duties in the capacity of Head of Entomology Division, with S. Wimaladharma relmquishing his services as the OIC. As per recommendation by the Director, the Tea Research Board decided to station the Head of Advisory and Extension Division at this station from this year in view of the fact that the tea lands in the low country contribute the most towards production and earnings from tea. The Tea Research Board accepted a recommendation by the Director to expand the services rendered by the station to the low country tea sector and accommodate the higher demand for research, extension and other services by the sector in keeping with the contribu­ tion it makes towards the total production and earnings from tea in the country. Plans were underway for this expansion. 1.1 Appoinments, Transfers and Retirements D. A. Lional, driver, was transferred from Head Office with effect from 20th January. K. Gunawardana was appointed as work Supervisor of the station with effect from 03rd February. D. V. Jemis, Plumber Mechanic retired from the services of the institute with effect from 07th June, after continuous service of 35 years at the station. S. L. D. Amarathunga, Extension Officer who acted for J. A. S. K. V. Jayasinghe in the capacity of officer-in charge at the TRI Advisory and Extension Centre at Deniyaya was transferred back to the Station in July and Mr. H. J. M. De Silva, Extension officer who acted for Mr. Amaratunga at the station returned to TRI Mid Country Station simultaneously. Ms. B. S. Vithana, Technical Assistant was transferred from Head office with effect from 1st March. T. S. Lokuhetti, Clerk/Typist was transferred from Head Office with effect from 26th December, to work in place of Mr. R. Nadarajah who was transferred back to Head office. ANNUAL REPORT FOR 1997 231 R B. Chandradasa, Technical Assistant was transferred to Biochem­ istry Division, TRI Talawakelle with effect from 27th November. 1.2 Human Resources Development - Local and Overseas : S. L. D. Amarathunga completed the academic part of an MSc programme in November and commenced the project component which is an agro-ecological survey : "Impect of Variation of the Weather param­ eters on Crop Environment and Productivity of tea lands in Sri Lanka". S. Koneshwaramoorthy, Mechanical Engineer, continued on the practical project on utilization of Solar Energy in tea factories as part fulfillment of requirements for a PhD. H. S. N. Pieris, Experimental Officer continued his research work as part fulfillment of the requirments for MPhil degree. S. M. Samarasinghe, Experimental officer continued with her research work on allomones (kairomones and allelomones) of tea and other plants with reference to Low Country Live Wood termites of tea, as part fulfillment of the requirements for MPhil degree. Overseas G. Galahitiyawa, Research Officer participated at an international workshop on "Renewable Energy Application to Plantation and other Industries" in India, in April. H. S. N. Peiris, Experimental officer followed a trining course on 'Tea Plantation Management" from July to October, in India. 2.0 Advisory and Extension Services Staff combination of one advisory officer and one extension officer in the Advisory and Extension Division continued in this year too. The Regional Scientific Committee of the region (RSC IV) remained inactive for want of reappointment of the committee and thereby, the scheduled programme of extension services could not be completed. The work output is summarized as follows: Advisory correspondence 626* Advisory visits made by the staff 139* + 16** a. Estates 45 Local b. Small holders c. Others (Extension works) Testing of soil samples for pH 39 53 569 232 ANNUAL REPORT FOR 1997 Extension Programs : Type of Programme Estate Small Diploma/ Other Sector Holder Student Sector a. RSC 3 1 - b. Seminars/Field Days 5 3 - c. NTPM Programs 4+6** - - d. Skill-trainning in nursery management 10 3 - e. Other trainning Programs - -20 f. Group discussions 11 12 - g- Informal discussions 4 11 3 h. Demonstrations using Community Participatory Adaptive Research trials (Pluckmg/manufacturing weeding, etc) - 8 - i. TRI/TSHDA interaction meetings - 4 - J- Educational Programs - 3 - k. Meetings attended by the staff on advisory matters - 15 Total number of extension programs conducted = 112 1. - Publications distributed in the low country : a. Advisory leaflets 560 b. Advisory Circulars 460 c. Handbook on Tea 06 d. Field Guide Book 20 e. TRI Monographs 06 m. Visitors to the station : a. Estate management personnel 103 b. Small holders 180 c. Students 70 d. Others 79 * - The number of activities conducted by staff of Advisory and Extension Division ** - The number conducted by staff of Technology Division S. Wimaladharma, S. Koneshwaramoorthy, G. Galahitiyawa, S. L. D. Amarathunga and research staff of other divisions. ANNUAL REPORT FOR 1997 233 3.0 Research Activities Research activities of the following projects were on-going in the low country and were serviced by the staff resident at the station except in the case of pathology studies: Agronomy Division Entomology Division Plant Breeding Division Technology Division Pathology Division Projects B/INCR and D/AGRY Projects B/TERM, D/ENTO and MeBr Project B/CLON Project D/TECH, ENGY Project D/PLPA Progress of work under the above research projects are being recorded in this report elsewhere. 4.0 Issue of Clonel Material for Establishment of Mother-bushes A total of 6500 clonal shoots were supplied to the estates for establishment of their mother bush blocks. The clones included: 4002, 4004, 4014, 4042, 4043, 4044, 4046, 4047,4049, 4052, 4054, 4053, 4059, 4061, 4062;, 3041, 3046, 3047, (immune to low- country live-wood termites but, highly prone to stem canker caused by Macrophoma theicola) 3052, 3055, 3058, 3060, 2025 and 2027. The above issues varied from one clone to twelve clones per recipient, supplying material for nursery plants varying from 125 to 3000 plants per clone, the higher number being of old clones. It is useful to record the identity of the recipients of the above clonal material for future reference. They are: Estate Sector: Aigburth Estate, Bulutota; Kelani Estate, Yatiyantota; Gekiyanakanda Estate, Neboda; Pelawatta Estate, Pelawatte; Yatideriya Estate, Undugoda; Vbgan Estate, Matugama; Pambegama Estate, Dehiowita; Small Holder Sector : D. Perera, Mathugama; K. B. Prematilaka, Kuruwita; Chaminda Kumaranayake, Hidellana; Rajapakse, Ratnapura and Mahinda Liyanage, Ratnapua. A. K. M. Jayasena and N. Piyasundera 5.0 Meteorological Station: Recording of meteorological data and despatch of monthly data to the Meteorological Department continued. The summary of weather records for the current year is being reported elsewhere in this report, 234 ANNUAL REPORT FOR 1997 Sushila I. Vitarana 6.0 Station Administration A proposal to expand research, advisory and extension services capacity at the Low Country Station and engagement of Sri Lanka State Engineering Corporation (SEC) for consultation purposes were approved by the Tea Research Board. Planning commenced in April. It has been proposed to increase the Staff Cadre from 38 to 74 with a staff structure as given below: Grade 1 - 0 9 (research staff only) Grade U - 12 Grade III - 01 Grade IV - 23 Grade V - 16 Grade VI - 13 Surveying and preparation of the preliminary architectural drawings of the Entomology lab, Library, Plant Breeding lab, Stores and the Auditorium were completed in October. A total of Rs. 161,791,500/= has been estimated for the whole project inclusive of staff residential facili­ ties. A project proposal was submitted to Asian Development Bank (ADB) to request for funding for construction work and procurement of facilities. The proposal was accepted in principle by the bank for funding under the Second Small Holder Development Project scheduled to be undertaken in 1998. 7.0 Special Events: The first ever Experiments and Extension ( E & E ) Forum to be conducted in Sinhalese for the benefit of Sinhalese speaking majority particularly of the small holder sector, was held at the TRI Low Country Station on 5th December. Mr. Rohana Illangaratne, the Chairman of the TSHDA was the Guest-of-Honour. MID COUNTRY RESEARCH, ADVISORY & EXTENSION CENTRE KANDY 1. General: Mr.A.W.M.L.S Abeysinghe, Experimental Officer, was transferred from Talawakele to the Entomology Unit in September. Ms.U.Sritharan, Experimental Officer, was transferred from Talawakele to the Tissue Culture Unit in December. Mr.S.T. Yatawatte, Advisory Officer had undergone 3 month training in Tea Plantation Management in the Kothari Agricultural Management Centre, India from July to September. Mr.P.BEkanayake, Officer-in-Charge & Agronomist participated and read the Country paper at the Study Meeting on "Sustainable Farming Systems in Upland Areas" held in Japan from 27th October to 2nd November 2. Advisory & Extension Service: 305 Letters were written to estates and small holders 119 Advisory visits were made during the year 233 local and foreign visitors and 324 small holders visited the Station. 1629 Soil samples were tested for pH 04 RSC Seminars were held for Superintendents & Assistant Superintend­ ents in the region. 09 Field days were held for Estate Field staff at the Station and in several estates in the Mid Country. 07 Seminars/demonstrations were held for small holders in the region 3. Hectarage as at 31st December, 1997 Type of land use ha Seedling tea 3.60 VP tea (mature) 7.00 VP (young) 0.40 Mother bush 3.00 Nursery (tea) 0.20 Fruit trees 0.40 Coconut 0.81 Forestry 1.21 Marshy land 0.62 Buildings, gardens, paths & roads 5.77 Total 23.00 236 ANNUAL REPORT FOR 1997 4. CROP Green leaf harvested (kg) Month Crop Crop sold Rate paid/kg Total harvested Rs.cts Rs.cts January 3855 3854 11.40 43,935.60 February 2608 2604 12.92 33,643.68 March 1492 1486 12.81 18,099.48 April 4583 4496 12.63 56,784.48 May 4017 3992 16.29 62,504.73 June 2580 2715 16.80 45,612.00 July 2548 2540 14.70 37,338.00 August 3576 3565 15.43 55,007.95 September 3271 3241 16.08 52,115.28 October 4535 4461 17.30 77,175.30 November 4429 4418 18.70 82,616.60 December 3759 3746 18.50 69,301.00 Total 41,118 634,134.41 5. Income No:of cuttings sold Income from sale of cuttings No:of VP plants sold Income from sale of plants Total crop harvested (kg) Income from sale of green leaf Guest House occupation charges Electricity charges Soil testing (pH) charges Miscellaneous Total income 6. Special Scientific Visitors 1. Mr. James Tupper, NTPM, Colombo in January 2. Mr. D.M.K.Bandara NIPM, Colombo in January 3. Mr. Athauda Jayawardena, CIC (Colombo) Ltd.in April 4. Dr. K.P.Premaratna, Faculty of Agriculture University of Peradeniya in April 5. Ms. R.D.Chitranayana, NRMC, Department of Agriculture, Peradeniya in May 6,86,125 Rs. 1,37,225.00 96,121 Rs.3,26,811.40 41,118 Rs.6,34,134.41 Rs. 21,275.00 Rs. 2,940.10 Rs. 48,870.00 Rs. 39,492.50 Rs.1,210,748.41 ANNUAL REPORT FOR 1997 237 6. Mr. Saman Premalal, Monsanto in June 7. Mr. Gordon Tissera, Kahawatte Plantations Ltd. in June 8. Dr. Palitha Randeniya, University of Peradeniya in August 9. Dr. S.Kodomari, Shizuoka, Japan in August 10. Dr. M.Omori, Otsuma Wou University, Tokyo, Japan in August 11. Mr. Sarath Wimalaratna, Visiting Agent, SPC/JEDB in October 12. Mr. M.B.Ratnayake, Director, JEDB in October 13. Dr. L.Maniwel, Plant Physiologist, India in November 14. Dr. G.P.Shetty, Multiplex Fertilizer Ltd. India in November 15. Dr. (Mrs) Robini Ekanayake, Entomologist, ORDI, Gannoruwa in December DENIYAYA ADVISORY AND EXTENSION CENTRE DIYADAWA Actg: Officer-in-Charge: J.A.S.K.V. Jayasinghe General Mr. J.A.S.K.V. Jayasinghe returned to the Deniyaya Station on 01st July 1997 after completing his Post Graduate Diploma Course in India. Mr. H.K.S.L.D. Amarathunga was transferred back to Ratnapura Station with effect from 05 th July 1997. Staff Position as at 31st December 1997 Mr. J.A.S.K.V. Jayasingha Extension Officer/Actg:OIC Grade IV BSc(Agri) Mr. C.W. Jayawardana Station Assistant V Mr. R.W. Suwaris Field Supervisor VI Mr. P. Ranaweera Driver VI Mr. A. Sinnathamby GH Keeper VI Adviory & Extension Services 310 letters were written on Advisory matters 34 Advisory visits were made 51 Inspection visite (Experimental & Demonstration trials) Nil Commercial nursery inspections 578 Visitors including small holders visited the station 2 Field days (Small holders) 18 Training programmes (15-Planters, 8-Small holders, 2-Students, 1-Labourers & 2-Campus students) 13 Seminars (10-Small holders, 3-RSC) 2 Field demonstrations (1-Small holders, 1-Planters) 17 Informal discussionss (7-Planters, 7-Small holders, 3-Tea inspectors) 35 Vedio programmes Nil Exhibitions etc. participatted 87 Soil samples tested for pH Hectarage Area under tea 6.41 Ha. Area under Coconut 0.13 Area under Pepper 0.05 Building 1.44 Nursery 0.70 Encroachment 1.12 Jungle (Rock area) Total 1.45 1.92 13.22 Ha ANNUAL REPORT FOR 1997 239 Crop Green leaf harvested Month Harvested (Kgs) Rate paid/kg Total payment (WithA/CLON) Rs. Cts. Rs. Cts. January 1617 17/773 27,121.94 February 980 16/398 16,070.04 March 1643 17/14 28,161.02 April 1876 18/20 34,143.20 May 2194 19/148 42,010.71 June 1774 20/554 36,462.80 July 2368 19/788 46,857.95 August 2016 20/84 42,013.44 September 2390 21/178 50,615.42 October 1951 21/218 757 21/00 56,873.32 November 2901 20/107 58,330.41 December 2826 20/81 58,800.06 Total 25273 497,469.34 Income No of cuttings sold 131,100 Income from sale of cuttings Rs. 26,220 No of VP plants sold 22,450 Income from sale of plants Rs. 77,332.50 (with loading plants) Amount of crop harvested (Kgs) 25,273 Income from sale of crop Rs. 497,469.34 Average price fetched per kg Rs. 19,684 Miscellaneous income Rs. 27,022.09 Total income Rs. 628,043.93 Experiments which are being conducted at the station LVP-74 (30) LC - Phase H This trial commenced at the station in August '97. Project MeBr - MeBR -18 The experiment to determine the efficacy of soil solarization as a means of eradicating nematodes in nursery soil, at the TRI-Deniyaya station commenced in December '97. Project MeBR - MeBR -19 Sedimentetion process for nematodes infected water. This experiment was started during December 1997 at Deniyaya station. 240 ANNUAL REPORT FOR 1997 Shear plucking Vs Hand plucking A trial was commenced to study the effect of differnet methods of plucking (TRI shear, Indian shear & hand plucking) on yield of tea. This experiment is in progress. The experiments which are in progress out side the station under different projects with the assistance of TRI-Deniyaya station 1. Effect of Earthwarm on yield of tea (Anningkanda Estate) 2. The effect of application of Potassium and/or Magnesium from Sul-Po-Mag and Kieserite at 2 levels of N on Soil/Plant nutrient status and yield of tea (Kiruwananga Estate). This experiment is in progress. 3. MeBR-06 - To test alternatives to eradication of mematodes in tea fields. (Handford Estate). This experiment is in progress. 4. Effective time and types of pruning in reducing SHB (E-284). (Kiruwanaganga Estate). This experiment is in progress. 5. Effective combination of shade trees for diversification purposes (E-287) (Kiruwanaganga Estate). This experiment is in progress. 6. Demonstrative Fertilizer Trials - Pasgoda & Deniyaya This experiments has been carried out by TRI in collaboration with TSHDA (Matara region). This experiment is in progress. 7. LVP - 43 Diyadawa Estate Plucking continued from January 1997 and the yield records are maintained. UVA ADVISORY AND EXTENSION CENTRE - PASSARA Officer in Charge: M.B.A. Perera 1. Advisory and Extension Services. 294 Advisory letters were written 74 Advisory visits were made to Estates 19 Seminars/Field Days were held for Estates/Smallholders 6 Regional Seminars were held in collaboration with Uva RSC 304 Visitors including Planters/Smallholders visited the Centre 936 Soil samples were tested for pH. 400 Soil samples were analyzed for their organic carbon content 2. Hectarage as at 31st December 1997 (approximate) Mature tea in plucking - 5.30 Mother Bushes - 1.15 Under Rehabilitation - 0.50 Buildings /Roads - 0.50 Forest /Scrub/Grass /area - 6.85 Total 14.30 3. Crop Green leaf harvested and sold (kg) -1997 Month Harvested Sold Price/Kg Income January 3068 3074 11.50 35,351.00 February 2579 2578 12.22 31,503.16 March 2458 2455 12.45 30,564.75 April 3898 3898 12.65 49,309.70 May 5302 5303 14.34 76,045.02 June 2864 2862 14.57 41,699.34 July 3362 3363 13.83 46,510.29 August 1922 1920 15.06 28,915.20 September 2278 2277 16.74 38,116.98 October 3674 3676 18.66 68,594.16 November 3912 3930 19.06 74,905.80 December 2597 2598 18.95 49,232.10 Total 37914 37934 570,747.50 The average yield obtained, including the seeding area, was 1575 kg MT ha 1. The Estimated Yield for the year was 1413 kg MT ha 1. The average price obtained for green leaf during the year was Rs.15.05 per Kg. Green leaf prices rose markedly during the last quarter of the year. 242 ANNUAL REPORT FOR 1997 4. Income No. of VP Cuttings sold Income from sale of VP Cuttings No. of VP plants sold Income from sale of VP plants Amount Kg of crop harvested Income from green leaf sold Average price fetched per Kg green leaf Soil analytical charges 75,850 Rs. 18,962.50 60 Rs. 225.00 37,934 Rs.570,747.50 Rs. 15.04 Rs. 72,080.00 Other Income Guest House occupation, Sale of publications etc. Rs. 31,381.00 Rs.693,171.00 Total The nursery was maintained only to raise a limited number of TRI 3000/ 4000 series clonal plants. These plants were issued for clonal trials in plantations and selected smallholdings. A batch of seedlings are maintained in the nursery raised from selected seeds obtained from Poonagala seed garden. These seedlings will be planted at the Centre for selection by Plant Breeding/ Propagation Division. 5. Labour Force No of permanent check-roll labour 26 Outturn (Women) 74% Outturn (Men) 48% The very poor outturn of men labour hindered scheduled field operations. 6. Field Trials 6.1 Clonal observation trials UVP 8 and UVP 9 are in progress at the Centre. These trials are carried out and monitored by the Plant Breeding/Propagation Division. 6.2 An observation trial on the use of shears for harvesting tea is carried out at the Centre. 6 3 Demonstration trials on the use of fertilizer mixtures T-l 130 and U-709 for mature tea under small holder cultivation conditions are carried out in three different locations in the region in selected smallholder properties. These trials are conducted by the Soils/Plant Nutrient Division in collaboration with TSHDA. ANNUAL REPORT FOR 1997 243 7. Special Uva problems 7.1 Terminal die-back of VP bushes of about 25 years old (especially clone TRI 2023) is becoming a common problem in Uva. 7.2 Poor recovery after pruning was also reported by many plantations. This condition was mainly due to inadequate starch reserves at the time of pruning. Resting of fields due for pruning is not a standard practice in many Uva plantations. 7.3 Formation of variegated foliage on young tea bushes and on bushes recovering from pruning was a "New Problem" reported by many plantations, and some small holders as well. Investigations are in train to establish the reasons for this condition. 7.4 Weeds known as "Passali" (Talinum paniculatum) and "Passali kodi" (Andredera basseloides) are becoming serious problems in Uva. Special investigations by our Agronomy Division in collaboration with the University of Peradeniya are in progress regarding this problem. ADVISORY AND EXTENSION CENTRE KOTTAWA, TALGAMPOLA 1. General Mr. K.D. Dahanayake continued as Officer-in-Charge. 2. Improvements to the centare 2nd coat of tarring for the old approach road to the centre was carried out. 3. Labour force Number on check roll - 60 Outturn - 50 average 4. Crop Green leaf harvesting -1997 Month Harvested Sold Rate paid/kg Total Income kg kg Rs. cts. Rs. cts. January 7340 7340 17/88.6 131283.24 February 4772 4772 18/30.9 87370.55 March 4779 4779 18/10.4 86519.02 April 5042 5042 19/37.7 97698.83 May 6731 6731 20/83 140206.73 June 4722 4722 21/84.5 103152.09 July 6548 6548 20/73.5 135772.78 August 6583 6583 21/87.2 143983.38 September 8009 8009 22/00 176198.00 October 7783 7783 22/53.4 175382.12 November 6649 6649 22/00 146278.00 December 7060 7060 22/50approx. 158850.00 a] 76018 76018 1,582,694.74 Total rainfall _ 2805.1 mm No. of wet days - 165 5. Commercial nursery Income from supply of VP plants - Rs. 125982.50 Income from supply of VP shoots - Rs. 12350.00 6. Miscellaneous income Rs. 18060.00 ANNUAL REPORT FOR 1997 245 7. Total Income Income from sale of green leaf Income from sale of VP plants Income from sale of VP shoots Miscellaneous income Rs. 1582694.74 Rs. 125982.50 Rs. 12350.00 Rs. 18060.00 1,739,087.24 8. Extension services (I) Small holders Number who visited the station personally seeking advice and collecting VP plants and VP shoots 742 The number who requested for visits, small holdings visited - 81 Correspondence -114 (IJ) Training programme - Superintendents, Assistant Superintendents, Field Staff and small holders - 59 Seminars/Field days - Objective - Advice on all aspects including demonstration relating to nursery work, plucking, pruning, pests & diseases as applicable to Low Country - 45 9. No. of soil samples tested for pH - 171 10. No. of VP plants issued - 35995 11. No. of VP cuttings sold - 61750 12. Experiments & Observations 01. Chopping prunings 02. Shear plucking observations 03. Hedge planting observation block 04. 4000 Series clones observation block 05. Bud grafting experiment 06. Caliandra plants block 07. Intercropping coffee, pepper planted in existing tea 08. 200 Plants Cinnamon in boundaries (intercropping) 09. Biclonal experiment 10. Multi Vs single cutting planting observations 11. Gravilia robusta provanances shade trees 12. Adaptive Research Trials at Hiniduma, Akuressa & Elpitiya METEOROLOGICAL OBSERVATIONS - 1997 TRI - St Coombs, Talawakele (Lat. 6° 551 N, Long. 80° 40% 1382 m amsl) Mean Temperature (°C) Soil at 20 cm Relative Humidity Wind Mean Total under grass - (%) 1 -Travelled Sunshine Rainfall depth (miles) (h. day 1) (mm) Month Min Max 8.30h 15.30h 8.30h 15.30h January 9.3 25.7 21.4 22.5 90.5 80.9 2401.13 8.6 0.0 February 9.6 26.5 22.0 23.3 91.7 92.2 1984.20 8.1 8.5 March 10.2 27.4 22.6 23.8 90.5 92.9 2473.00 7.4 42.1 April 13.8 26.3 22.0 22.7 91.9 94.2 1761.00 6.1 337.0 May 15.1 25.4 22.1 22.7 94.0 94.5 1856.00 6.1 218.0 June 14.2 24.4 22.4 23.0 93.4 93.2 1943.00 6.6 154.5 July 16.4 22.9 21.8 22.2 96.7 94.7 2364.00 2.7 203.3 August 15.0 23.1 21.7 22.2 95.6 93.4 2787.00 5.6 137.4 September 14.8 23.6 21.8 22.3 92.9 95.1 6420.00 5.4 298.0 October 14.6 25.1 21.9 22.3 92.1 95.1 1759.00 5.8 425.5 November 14.6 24.8 22.1 22.5 93.3 94.4 1807.00 5.4 298.0 December 14.6 24.5 21.7 21.7 22.2 93.8 2319.00 5.2 164.4 MID-COUNTRY, RESEARCH, ADVISORY AND EXTENSION STATION, KANDY (Elevation 762 m amsl) Month Mean temp. Relative Humidity Mean No. of Cum. (°C) ( ;%) sunshine Rainfall wet days Evaporati Min. Max. 08.30 h 15.30 h (h. day1) (mm) (mm) January 18.06 26.79 86.73 78.06 8.76 0 0 111.70 February 18.59 29.52 78.55 64.28 7.25 16.80 3 119.80 March 19.61 30.34 82.72 58.79 9.02 85.10 4 121.70 April 20.02 29.85 85.91 82.27 6.76 326.00 19 108.30 May 21.00 28.83 87.24 85.51 6.77 173.40 16 74.90 June 20.67 29.26 85.30 79.06 6.73 72.91 11 105.90 July 20.98 26.65 88.41 84.47 4.29 112.55 16 65.80 August 20.66 26.45 88.83 81.86 6.62 86.00 11 108.70 September 19.85 27.74 86.35 84.44 5.43 317.90 21 74.50 October 19.66 27.76 92.71 92.03 5.05 503.00 19 85.90 November 19.88 27.83 95.41 90.52 4.65 391.10 23 47.60 December 19.44 27.60 95.93 91.11 5.43 211.70 14 9.50 LOW COUNTRY STATION, RESEARCH,ADVISORY & EXTENTION CENTRE,RATNAPURA -1997 (Lat 6° 41 N Long 80°E - 40°E, 29m amsl) to 00 Mean Temperature Relative Humidity (%) Mean Total Difference Wet Difference Evaporati Sunshine Rainfall from days from (mm/da; Max. Min. 9.00h 16.00h (h day 1) (mm) 30yrs 30yrs Month Dry. Dry. January 23.4 34.4 84 59 7.5 9.0 - 102.9 02 - 0 7 4.39 February 24.3 35.1 82 53 6.9 47.2 -89.8 04 - 0 5 4.65 March 24.6 35.2 84 57 6.5 164.0 -48.2 09 - 0 5 5.00 April 24.4 34.8 84 68 6.0 401.8 + 62.9 19 -01 3.47 May 24.8 33.4 84 79 4.6 574.2 + 98.3 20 No difference 2.44 June 25.2 33.1 82 72 5.8 331.8 -80.4 12 - 0 9 3.50 July 24.3 31.4 87 73 3.2 601.2 + 308.4 24 + 04 2.39 August 24.9 31.8 84 71 5.4 182.0 - 122.1 17 -03 3.39 September 23.6 32.1 86 75 3.4 721.3 + 299.9 19 -01 2.34 October 23.7 33.2 86 82 3.7 569.1 + 132.3 27 + 06 2.68 November 23.7 32.7 86 79 4.1 591.4 + 220.0 21 + 03 2.27 December 23.2 32.9 87 77 4.0 384.6 + 149.3 20 + 06 . 2.43 Total _ _ . . - 4577.6 - - _ Mean 24.2 33.3 85 70 5.1 - - - - 3.24 > z z c > r tn -o O Tl o 70 - J DENIYAYA ADVISORY AND EXTENSION CENTRE (Elevation 250 m amsl) Mean Temperature (°C) Soil at 30 cm Relative Humidity 1 % Total Total Sun shine* Total Mini Dry Max Dry 0830 h 1530 h 0830 h 1530 h Rainfall wind Evaporation Month (mm) (km) January 17.7 27.7 27.7 27.8 66.22 53.23 29.9 783 February 20.4 32.5 27.5 28.0 77.48 60.71 200.8 700 - March 26.5 33.9 28.9 29.4 79.23 62.70 89.7 838 - April 22.0 32.6 28.5 29.2 79.23 72.46 498.0 695 - May 23.3 31.1 28.0 29.1 82.0 60.64 528.5 1043 - June 23.0 31.6 29.0 29.2 79.74 67.81 155.1 1274 - July 23.8 30.1 27.9 28.2 79.82 73.29 392.0 2087 - August 24.4 30.0 27.8 28.7 78.77 70.19 193.6 2519 - September 23.1 30.3 27.6 27.2 83.0 77.82 567.0 1226 - October 22.0 31.5 28.2 28.5 82.9 84.00 403.4 751 - November 20.7 31.7 27.0 27.6 81.7 80.1 723.9 491 - December 20.8 30.8 27.4 28.3 80.7 69.5 563.7 961 - Total - - . . _ _ 3907.4 13168 Mean 22.31 31.15 27.96 28.40 80.69 70.21 _ _ > z z c > r jo m •A o o NO NO ^ ] * Sunshine recorder had been stolen to NO UVA ADVISORY AND EXTENSION CENTRE - PASSARA -1997 (Lat. 6°56' N, Long 18°07 88.1 - 10.7 03 - I 74.0 -.63.5 05 - £ 309.2 + 46.0 11 - g 260.1 - 112.4 15 - | 183.3 - 117.5 15 - £ rs 299.6 + 115.8 23 - g 172.7 - 35.8 18 - § 434.1 + 158.0 20 - 426.0 +72.7 20 408.3 +90.1 20 145.7 -25.8 13 Total 2805.1 165 Mean 31.4 22.5 89 5.8 to Telephone 6 9 7 4 5 1 6 9 1 151 AUDITOR-GENERAL'S DEPARTMENT INDEPENDENCE SQUARE, COLOMBO 7, SRI LANKA atawAr a ^ B a s u i , Ctentgiiii 7, ®>»mw»Ti My No. PI/H/TRB/97/171 ?23xa /^ l«$/Date: . 17 November 1998 S J U g l ® 6 0 . Your No. The Chairman, Tea Research Board. Report of the Auditor General in terms of Section 13 (7) (a) of the Finance Act No. 38 of 1971 on the accounts of the Tea Research Board for the year ended 31 December 1997 The above mentioned report is sent herewith. 2. In this connection your attention is drawn to Section 13 (8) of the Finance Act reproduced in the note below. (J.D.R. Jayalath) Assistant Auditor General for Auditor General. Copies : 1. Secretary - Ministry of Public Administration, Home Affairs and Plantation Industries. 2. Secretary - Ministry of Finance and Planning. Note: Section 13 (8) of the Finance Act "The reports referred to in paragraphs (a) and (b) of Sub-section (7) shall be consid­ ered by the governing body of a Public Corporation and after such consideration that body shall inform the Auditor General of the steps that they propose to take with regard to the matters pointed out in the audit reports within three months of the sub­ mission of the reports to the Corporation." PI/H/TRB/97/171 17 November, 1998 The Chairman, Tea Research Board. Report of the Auditor General in terms of Section 13 (7) (a) of the Finance Act No. 38 of 1971 on the accounts of the Tea Research Board for the year ended 31 December 1997 The audit of accounts of the Tea Research Board for the year ended 31 December 1997 was carried out under my direction in pursuance of provisions in Article 154 (1) of the Constitution of the Democratic Socialist Republic of Sri Lanka read in conjunction with Section 13 (1) of the Finance Act No. 38 of 1971. My report in terms of Section 13 (7) (a) of the Finance Act appears below. 1.2. Scope of the Audit Audit comments and findings in this report are based on a review of the financial statements presented to audit and substantive tests of samples of transactions. The scope and extent of such review and tests were such as to enable as wide an audit coverage as possible within the limitations of staff, other resources and time available to me. The audit was planned and carried out in accordance with generally accepted auditing standards, methods and practices to obtain reasonable assurance as to whether the financial statements are free of material misstatements. The audit included examination of evidence supporting the amounts and disclosures in financial statements and assessment of accounting principles and significant estimates and judgements made in the preparation of financial statements, evaluation of their overall presentation and determining whether accounting policies adopted were appropriate, consistently applied and adequately disclosed. Subscriptions (3) and (4) of Section 13 of the Finance Act No. 38 of 1971 give discretionary powers to the Auditor General to determine scope and extent of the Audit. 2. Accounts 2.1. Financial Results According to the accounts presented, the working of the Tea Research Board for the year ended 31 December 1997 had resulted in a surplus of Rs. 35,309,603 compared with the surplus of Rs. 36,392,435 in the previous year. The following statement gives a summary of the financial results for the year under review and the preceding year 255 Year ended 31 December 1997 1996 Rs. Rs. Rs. Rs. Income Cess and grants 94,433,902 86,027,473 Income from plantation and other commercial activities 33,910,940 27,118,483 Miscellaneous income 4,279,304 2,612,752 Interest on investments 3,226,181 135,850,327 3,603,803 119,362,511 Less: Expenditure Personnel emoluments 31,120,839 25,654,070 Travelling expenses 4,285,504 2,902,200 Supplies and requisites 9,476,067 8,924,263 Repairs and maintenance of capital assets 13,012,056 8,805,953 Depreciation 8,300,297 8,896,950 Transportation, communication, utility and other services 15,746,117 13,159,130 Losses and write-offs 29,238 4,976 Media, advertising, publicity symposia and gifts 474,130 437,180 Contributions, grants and subsidies 1,431,020 1,191,515 Pensions and retirement benefits 3,312,759 2,673,829 Provision for Gratuity 4,827,854 1,018,949 Cultivivation and experiments 4,328,305 4,050,270 Miscellaneous 4,196,538 100,540,724 5,250,791 82,970,076 Surplus for the year 35,309,603 36,392,435 Prior Period Items (112,493) (1,311,017) Transferred to Tea Research Fund 35,197,110 35,081,418 22. Financial Structure According to the accounts presented, the financial structure of the Board as at 31 December 1997 compared with that as at end of the preceding year is given below. 256 As at 31 December Resources Government Grant Capital Reserve Long Term Liabilities - Land Reform Commission - Provision for Gratuity Tea Research Fund Utilization Fixed Assets at written down value Capital work-in-progress Development Projects Net Current Assets 1997 Rs. 1996 Rs. 1,175,000 40,196,431 754,670 17,117,790 213,441,710 272,685,601 1,175,000 38,324,941 754,670 11,327,641 178,244,600 229,826,852 113,507,235 6,747,175 8,795,003 143,636,188 272,685,601 105,376,827 1,022,786 8,795,003 114,632,236 229,826,852 Less: Gratuity paid - 695,393 Cash generated from Operating activities 19,496,512 21,485,343 Cash flows from investing activities Interest on Investments 3,226,181 3,603,803 Purchase of fixed assets (19,488,631) (12,573,600) Proceeds from sale of fixed assets 2,026,440 (Increase)/Decrease in capital work-in-progress (5,724,389) 691,205 Cash used in investing activities (19,960,399) (8,278,592) Cash flows from financing activities Grants received from ARP 561,491 2,733,084 NORAD 1,310,000 NARESA - 7,200 ACITR - 175,000 IWEM - 6,150 Purchase consideration of Lamilier Estate - 1,504,670 Cash generated from finance activities 1,871,491 4,426,104 Net increase in cash and cash equivalents 1,407,604 17,632,855 Cash and cash equivalents at beginning of the year 44,434,130 26,801,275 Cash and Cash equivalents at end of the year 45,841,734 44,434,130 1,407,604 17,632,855 257 23 Cash Flow According to the accounts presented, the cash flow of the Board during the year under review and the preceding year is given below. Year ended 31 December 1997 Rs. Rs. Cash flows from operating activities Surplus for the year excluding interest on investments Adjustments for items not involving movement of cash Depreciation Provision for gratuity Less: Income from sale of fixed assets 10,434,117 5,790,149 16,224,266 1,102,334 Less : Prior period items Operating surplus before changes in items of working capital Changes in items of working capital Stocks - (Increase)/Decrease (1,881,220) Debtors and other balances - crease) (26,090,999) Deposits, prepayments and purchase advances - (Increase) (1,332,526) Loans and Advances to staff & employees - (Increase)/Decrease (2,330,741) Other current assets - (Increase) (5,000) Identified losses - (Increase) Suspense - (Increase) Excesses and shortages - Decrease Creditors and provisions - Increase (156,507) (10,677) 4,394 19% Rs. Rs. 32,083,421 32,788,632 12,131,932 1,018,949 13,150,881 15,121,932 47,205,353 112,493 47,092,860 13,150,881 45,939,513 1,311,017 44,628,496 180,291 (23,080,795) (4,146,320) 104,031 (109,331) (135,410) 438 4,206,928 (27,596,348) 4,739,338 (22,447,758) 19,496,512 22,180,738 258 Format - 1 Methods and Formulae used in Estimating Physical Quantities of Materials Description of Materials Green leaf Whether receipts/ issues of inventory Receipts Description of formulae or method Whether the basis is realistic Factory weight of green Realistic leaf is ascertained after deducting 3% of the field weight for moisture Items in accounts based on Estimates and Provisional Valuation Whether there was a change in the basis No. Format - 2 Description of item Value Basis of estimating or in the accounts Rs. provisional valuation (a) Provision for payments 119,774 Actual payment made in subsequent year (b) Provision for Audit Fees 215,000 - d o - (c) Provision for bad debts 98,953 - d o - (d) Provision for Goods in transit 156,124 - d o - (e) Provision for Uniforms & Protective Clothings 250,000 - d o - (f) Provision for Holiday pay & Total earnings for the Year x Incentive Bonus 233,378 Entitled days Total days worked for the year (per employee) (g) Provision for Gratuity 17,117,790 Half month salary x No. of years of services. Basis of costing Receipts and Issues of Materials Type of material Whether receipts/issues (i) Stocks (ii) Green (Tea) Leaf (iii) Tea Chests (iv) Fire wood Receipts/Issues - d o - - d o - - d o - Format - 3 Basis adopted First in First out - d o - - d o - - d o - 259 Format - 6 Fixed Assets - Depreciation Rates Type of Asset Method of depreciation Rate per annum 1997 7996 (i) Buildings Straight line method 2% 2% (ii) Motor vehicles - d o - 20% 20% (iii) Laboratory equipment - d o - 20% 20% (iv) Library books - d o - 10% 10% (v) Water Supply - d o - 10% 10% (vi) Furniture - d o - 10% 10% (vii) Office equipment & computer - d o - 10% 10% (viii) Electrical installations - d o - 10% 10% (ix) Work Shop Equipment - d o - 10% 10% (x) Telephones - d o - 10% 10% (xi) Capital replanting - d o - 3% 3% Note : Depreciation is provided for the year of purchase and no depreciation is provided in the year of disposed Format-9 Accounting Deficiencies Description of item Type of Description of deficiency in the accounts deficiency affected (a) Work in progress Inappropriate Disclosure The work had to be carried out under several research projects which had bi^ en abandoned subsequently are continuously shown under the work-in-progress account for over 7 years. The details of the projects are given below Rs. NERD L/C Technology 190,369 Instant tea Project 4,652,830 LTP Project 4,142,173 Water Tank H/O 192,855 Water storage St. Joachim 104,014 9,282,241 260 (b) Fixed Assets Errors in Capital and revenue recognition Description of the asset (a) Supplying & Installation of Auto Air Condition System to the Pajero No. 32 - 5991 (b) Supply of 03 Nos. Termite Detectors (c) Stock of fuel in vehicles Overstatement (d) Fixed Assets Understatements/ Depreciation Overstatement Nature of Assets Division Machinery St. Joachim estate Machinery - do - Following fixed assets pur­ chased by the Board had been written off without con­ sidering their capital nature. Name of Value the account charged Rs. "Repairs and maintenance of capital assets account" 35,000 "Supplies & Requisites account". 45,000 Fuel stock valued at Rs. 18,950 as at 01.01.97 of the vehicle fleet of TRB had not been deducted to reach the balance stock as at the end of the year. Balance stock, therefore, shown as Rs. 41,773 as at 31.12.97 had been overstated by Rs. 18,950 as well. Following understatement/ overstatement of deprecia­ tion of fixed assets had been revealed in the accounts due to calculation errors. Overstatement/ Understatement Rs. 246,786 (Overstatement) Rs. 154,786 (Understatement) 261 (e) Fixed Assets Errors in Capital and revenue allocation Following expenditure had been capitalised under various fixed assets items without considering their recurrent nature. description Cost of binding of journals Amount Account debited Rs. 19,650 Library books Annual subscription fees of "Economics" 6,240 Other fixed assets (f) 7 day call deposits Omission Accrued interest for 1997 valued at Rs. 172,623 on 7 day call deposits had not been accounted for. (g) Rental Omission A sum of Rs. 90,000, being rent receivable from the Electricity Board, had not been shown in the accounts and no provision had also been made thereon. (h) Bonds Outstanding Omission Eventhough following officers had not resumed for duties at the Board after completion of their post graduate degrees at foreign countries, the value of bonds aggregating Rs. 4,132,501 had not been brought to account. Name of officers T. S. Gunasekara Nalin L. Herath W. A. D. P. Wanigasundara D. K. Nawarathna Value of bonds outstanding Rs. 2,382,496 260,157 891,000 598,848 4,132,501 262 (n) Provision for Inappropriate gratuity disclosure (o) Tea Sales Overstatement (p) Work-in-progress Inappropriate disclosure (q) Electrification Labour Lines - St. Coombs Estate Classification error Provision for holiday pay amounted to Rs. 332,885 and Rs. 1,103,268 in respect of St. Joachim and St. Coombs Estates respectively, had been shown together with provision for gratuity under long term liability instead of being shown as current liability. Sales invoice No. 330 dated 30,12,1997 valued Rs. 123,624 had been accounted twice and thereby sales have been overstated by the same account. Advances amounting to Rs. 100,000 paid for the purpose of carrying out of a research Project had been shown under work in progress account the heading called "Collaboration Project". An estimated sum of Rs. 295,000 had been provided in the accounts and debited to the work in progress account for construction of labour lines at St. Joachim estate, without ascertaining the quantity of work completed at the end of the year under review. Deposit amounting to Rs. 145,600 made for the electrification of labour lines at St. Coomb's estate had been shown under the receivable from the staff in the accounts for the year under review. 263 Format -10 Verification of Physical Assets Class of Asset Percentage Shortages Excesses Value of assets Whether Treatment of verified observed observed not based on variations variations during the physical have been in year or at the verification investigated account TRB - Head Office end of year Rs. Rs. Rs. Rs. (i) Stationery stocks 100% 1,117 2,891 Nil No (ii) Motor spares 100% Nil Nil Nil No (iii) Scientific Stocks 100% 197,540 80,241 Nil No (iv) Drugs 100% 263 768 Nil No (v) Electrical Stocks 100% Nil Nil Nil No (vi) General Stocks 100% Nil 54,400 Nil No (vii) Stocks at Ratnapura (Low Country Stations) 100% 11,064 17,448 Nil No S t Coomb's Estate Consumable Stock Verified Schedules had not been submitted 1,640,881 - Verification conducted by the Govt. Audit Team Auto - Diesel 100% - 1,267 (lit.) No Fire - Wood 100% 18.4 (cm) - No Tea BOP (kgs) 100% 1,339 - PEKOE (Kgs) 100% - 6 Investigations in Progress BOPF (Kgs) 100% - 109 S t Joachim Estate Consumable stock - Verification Schedules had not been submitted to audit 844,818 - Confirmation of Assets Balance Type of assets as per accounts Total No. of accounts confirmed Format -11 No. ofValue of balancdtfo. of accounts Percentage accounts confirmed confirmed of balances as a confirmed percentage in value Rs. Rs. Rs. TRB - Head Office (i) Sundry debtors 1,535,850 109 Nil Nil Nil Nil (ii) Trade debtors 82,218,312 29 10 58,652,325 34,48 71.34 (iii) Analytical charges due 443,214 173 38 76,390 21.96 17.23 (iv) Insurance claims due - vehicles 103,085 7 Nil Nil Nil Nil (v) Pre-payments 456.032 5 Nil Nil Nil Nil (vi) Advance payments 4,762,593 95 1 25,000 1.05 0.52 (vii) Deposits 867,192 31 Nil Nil Nil Nil (viii)Bank Balances 9,908,953 3 2 4,035,079 66.67 40.72 (ix)Petty cash balances 310,089 8 Nil Nil Nil Nil 264 St Joachim Estate (i) Sundry debtors 34,772 26 Nil Nil Nil Nil (ii) Cash in hand 33,857 1 Nil Nil Nil Nil (iii) Pre payment 221,256 9 Nil Nil Nil Nil Confirmation of Liabilities Format -12 Balance Total No. ofValue of balancd&o. of accounts Percentage as per No. of accounts confirmed confirmed of balances Type of liability accounts accounts confirmed as a confirmed percentage in value Rs. Rs. % Rs. TRB - Head Office (i) Sundry creditors 2,597,753 409 25 551,903 6.11 21.24 (ii) Tender deposits refundable 15,450 20 Nil . Nil Nil Nil (iii) Security deposits refundable 179,378 3 Nil Nil Nil Nil (iv) Petrol deposits refundable 9,600 53 5 800 9.43 8.33 (v) Retention money payable 5,036 1 Nil Nil Nil Nil (vi) Other creditors 4,839,792 9 Nil Nil Nil • Nil St. Joachim Estate Sundry creditors 794,233 27 2 84,708 7.41 10.66 265 Unreconciled Control Accounts Description Control Balance as per Account Account TRI - Head Office (i) Depreciation 8,300,297 (ii) Electrical stock 866,118 (iii) Stock at TRI Ratnapura 281,202 (iv) Stock at TRI Kottawa 17,702 (v) Stock at TRI Deniyaya 91,473 (vi) V. R Plant Stock at Hantana 136,558 (vii) V. P. Plant Stock at Kottawa 136,558 (viii) Motor Spare Parts Stocks 1,348,549 (ix) Scientific stocks 887,473 (x) Labour loans - Deniyaya 7,100 St Jochim Estate (i) Depreciation 1,205,898 (ii) Fiexed Assets - Additions during the year 1997 5,011,381 (iii) Deepavali Festival Advance -1997 166,000 (iv) New Year Festival Advance -199713,200 St Coombs Estate (i) Account Current 721,887 (ii) Cash advance 25,350 (iii) Electricity Recovery - St. Coombs Estate 1,594,938 (iv) Electricity Provision Nil (v) Lorry 26/9375 Nil (vi) Electricity recovery - Lamilere Division 185,787 (vii) Other Income Nil (viii) One day Wages A/c 32,375 Format -13 Description Balance as per Difference of subsidiary subsidiary records records Rs. Rs. Detailed schedule 9,344,148 1,043,851 -do- 907,708 41,590 -do- 332,032 50,830 -do- 52,675 34,973 -do- 56,500 34,973 -do- 183,248 11,192 -do- 145,184 8,626 -do- 1,240,227.85 110,000 -do- 839,856 47,617 -do- 3,900 3,200 -do- 1,165,412 40,486 Fixed Assets 5,040,511 29,130 Register Detailed Schedule 174,800 8,800 -do- 5,200 8,000 Estate Balance 1,293,974 572,087 Sheet -do- 27.050 1,700 -do- 1,580,833 14,105 -do- 242,807 242,807 - d o - 18,561 18,561 - d o - 161,537 24,250 - d o - 821,050 821,050 - d o - 56,625 24,250 266 Suspense Accounts Title of Account (i) Suspense Account H.0 Nature of Transaction Recoverable amount from store keeper of the Board Loss of stationery items Balance as at balance sheet date Rs. Reason for not clearing Format -14 Age analysis Not Over four years explained (ii) Suspense Account - Unidentified payments St. Coomb's Estate by Sundry Creditors Lack of Documentary Evidence for Audit 169,285 178,791 Description of items in the accounts affected (a) Stocks (i) Stock at Estates St Coomb's Estate Consumable Stock Value shown in the accounts Rs. Not Over three years explained Format -15 Documentary evidence not available sheets Nursery Stock St Joachim Estate Consumable Stock Nursery stock 1,640,881 289,140 844,818 405,798 Detailed schedules and verification -do- Detailed schedules and verification sheets -do- (b) Debtors (i) H.O. Sundry debtors 1,535,850 Insurance claims due vehicle 103,085 Sundry debtors (26,308) Recovery A/c 115,609 (ii) St Coomb's Estate Staff salaries 158,074 Checkroll debtors 129,728 Sundry debtors 885,095 Ceylinco Insurance 10,530 Sub contractors 436,057 Electrification Labour Lines 1,409,151 Death donation A/c 12,487 Confirmations Confirmations Age analysis -do- Detailed schedules -do- Detailed schedules, age analysis and confirmations -do- -do- Detailed schedules -do- 267 (c) Deposits, Pre-payment and Advances (i) H.O. Deposits Pre payments (ii) St Joachim Estate Pre payment Gas Cylinder deposits Drugs purchase A/c (iii) St Coomb's Estate Purchasing advances (d) Loans and Advances (i) St Coomb's Estate Festival loan Special loan Staff loan Labour advance (e) Cash and Bank balances (i) H.O. Bank of Ceylon - Talawakele 9. Petty cash imprest at sub stations 867,192 Confirmations 456,032 Confirmations not called for 221,256 Confirmations 11,700 Detailed schedules 5,646 - do - 51,179 Detailed schedules and confirmation. 216,660 24,950 898,420 58,155 056,487 167,410 (ii) St Coomb's Estate Cash at Bank (f) Creditors, Provisions and Other Credit balances (i) H.O. Retention money payable Provision for Payment Provision for Goods in transit Tender deposits refundable Security deposits refundable (ii) St Coomb's Estate Sundry Creditors 1 Check Roll 1 Unpaid wages Attendance bonus Refundable deposits Provision for Payment Bought leaf A/c (iii) St Joachim Estate T. S. H. D. A. Plants Production bonus Provision for payments 1,555,957 5,036 119,774 156,124 15,450 179,378 ,660,190 ,038,720 7,120 595,646 13,598 11,500 952,416 60,900 227,279 1,954,652 Detailed schedules -do- -do- -do- Confirmations -do­ do- Schedule & confirmation Schedule -do- Confirmations -do- Schedules, age analysis & confirmations Schedules -do- -do- Schedules & Confirmations Schedules -do- Schedules -do- -do- 0j) Ratnapura Low Country station 12,312 Bills, Purchasing Order, GRN etc. 268 Age Analysis of Debtors Class of Amount 0-3 3-6 6-9 9-12 1-2 2-3 3-4 Debtor as at Months Months Months Months Years Years Years Balance Sheet Date Rs. Rs. Rs. Rs. . TRB - H.O Sundry Debtors 1,562,157 911,502 83343 38£78 Sundry debtors (26307) Age analysis had not been submitted Trade debtors 82218312 82201,031 Rs. Rs. Rs. Rs. Format -16 4-5 Over 5 Years Years Rs. Rs. 46355 175,482 10321 117,474 57214 121,488 13,437 3,444 Analytical Changs Due 443214 Insurance •aims Due 5133 Bonds Outstanding 2172,176 99395 60,665 9250 61,690 135334 39340 20,140 - 2377 400 12075 "4325 - 48#6 - 2,172,176 86,447,193 83212,428 144,008 48228 108,045 326,631 53,105 137,614 69289 2347,845 St Joachim Sundry debtors 34,772 (14,876) 13270 750 23,737 8297 3594 Format -17 Age Analysis of Creditors Class of Creditor Amount 0-3 3-6 6-9 9-12 1-2 2-3 3-4 4-5 Over 5 as at Months Months Months Months Years Years Years Years Years Balance TRB • H.0 Sundry Creditors StJoachim Estate sheet date Rs. Rs. Rs. 2,597,753 2,597.753 Sundry Creditors 794,233 791,608 Rs. Rs. Rs. Rs. Rs. Rs. Rs. 2,625 269 Format -19 Identified Losses Particulars of Items Amount Rs. (a) Petty cash loss 76,436 (b) Loss of salary packets 132,238 (c) Unpaid travelling claims 3,778 (d) Unpaid medical claim 2,221 (e) Unpaid cash against cheques 1,500 (f) Unpaid vouchers 200 (g) Loss of items from electrical foreman's bungalow 109,331 fli) Missing items from Divisions 156,507 (i) Loss of VP Plant 227,116 Sales-Deniyaya Investigated by the Internal Auditors Reasons for loss Loss of petty cash at cash counter in 1992 Treatment of loss in accounts Amounts identified had been transferred to the identified losses account and shown under the current asset. Loss of salary packets at the cash counter in June 1992 Advance not settled over 4 years - d o - A personal cheque encashed at the counter Advances not settled Assets removed by the officer who had occupied the bungalow Action had not been taken to recover the amount over 5 years and the amount recov­ erable had been transferred to identified losses account. do­ do- -do- -do Action had not been taken to recover the losses over two years. Loss of office and lab equipment investigated Action had not been taken to recover from by the Internal Auditors relevant officer. Reasons for loss had Adjustments had not been not been investigated made in accounts. (j) Missing inventory 8,741 items of Entomology & DDR (T)'s office investigated by the Internal Auditors - d o - -do 270 Format - 22 Format - 25 Other Slow-moving, Idle and Under-utilised Assets and Idle Labour Reason Description of item Estate Bungalow at St. Joachim Electrical Stock, General Stock H.O Period of non-use or under-utilisation Over three years Over three years Tea Factory at Lamilear Since 1987 Division - St. Coomb's Estate Vehicles Factory Mangers's bunglow at St. Joachim Estate had remained idle with the furniture for over three years. According to the report of the Board of survey for 1995 revealed that large number of items had remained in the stores without being utilised for appropriate purposes. A Tea Factory with the machineries installed for Production of green tea had remained idle from the time of acquisition of Lamilear Division by the Board. Following unserviceable vehicles had remained in the garage premises for long period without action being taken to disposed off. Registration No. Duration at garage 23 -7607 Tata -1210 Bus From 1993 29 - 6584 Isuzu van - d o - 35 - 1702 Mitsubishi van - d o - 38 - 8248 Volkswagen van - d o - 14 - 8959 Volvo car - d o - 27 - 4862 Mitsubishi Canter Lorry - d o - 41 - 4802 Mitsubishi Fuso Lorry - d o - 31 - 8906 Mitsubishi Jeep - d o - 40 -1990 Datsun Double Cab - d o - Generator at St. Joachime One year This generator had been remained idle for over one year. 271 Cost of Personnel Category of Personnel No. of Employees Total Cost Cost per Employee As at As at Normal Pay Overtime Normal Pay Overtime end of end of 1997 1996 1997 1996 1997 1996 1997 1996 1997 1996 Rs. Rs. Rs. Rs. Rs. Rs. Rs. Rs. Executives 52 54 5,956,224 6,166,789 - - 114,543 114,119 Nor>executives 119 112 8,649,626 7,527,482 729,048 654,413 72,686 67209 6,126 5,843 Minor Employees 62 58 3224,424 3,021,040 722,028 484,165 52,007 52086 11,646 8348 Uneconomic Transactions Class of Transactions (a) Gasifier and steam boiler/radiator system for withering and drying Project at St. Joachim Estate-Ratnapura (b) Foreign Scholarships Format - 26 Details of Transaction An Advance amounting to Rs. 2,043,750 paid to M/s Colombo Commercial Company Ltd. for supply & installation of gasifier Steam boiler at St. Joachim Estate - Ratnapura Employees of the Board who went on study leave abroad had not resume duties at the Board as per their bonds agreed. The value of the bonds to be recovered as follows. Nature of deficiency Eventhough the advance paid, the work had not been completed yet. Action had not been taken by the Board to get complete the contract or to recover the advance paid. The Board did not benefited efited in turn eventhough it had facilitated to the employees to enhance their technical skills and competent. The bonds outstanding valued at Rs. 5,952,477 remained unrecovered as at 31 December 1997. Name of the officer Value of the bonds outstanding Rs. Athula Ekanayake 317,370 U. K. K. Wickramasinghe 422,020 Sri Ramaratnam 125,325 Asoka Basnayake 104,261 T. Thevadasan 851,000 T. S. Gunasekara 2,382,496 Nalin L. Herath 260,157 W. A. D. P. Wanigasundara 891,000 D. K. Nawaratna 598,848 5,952,477 (c) Patent Rights (d) Insurance claims due Patent right had been registered by the Board in connection with the two inventions called "Development in or relatting to the increased stability and self life of liquid tea and concentrates and carbonated non carbonated ready to drink beverages" and "An efficient method for the commercial propergation of tea by Tissue culture technique." Insurance claims aggregating Rs. 51,333 had remained outstanding for over 8 years Eventhough this patent rights had been obtained several years ago and being renewed yearly income on the patent right had not been generated so far, thus indi eating that no one had been used these new inventions for their purposes. Eventhough these claims outstanding over long period, action had not been taken by the Board to write off the amounts. 272 Format - 28 Assets not budgeted. (B) Expenditure 1) Personnel Emoluments 30,229,000 50,000 30279,000 31,120,839 841.839(A) 2.78 Increase in daily rate of Labour 2) Travelling Expenses Local 2300,000 30,000 2330,000 3510,403 1,180,403 (A) 5132 Increase in Subsistence & batta rates and more visits Overseas 600,000 600,000 767345 167345(A) 27.89 More foreign visits 3) Supplies and Requisites 9,163,000 181,000 9344,000 9,476,067 132,067 (A) 1.44 Price increase of the General Goods etc. 4) Repairs and Maintenance of Capital Assets 8,458,000 57,000 8515,000 13,012,056 4,497,056 (A) 53.17 Repairstovehicleswmchareover 10 years old & due to high cost of Spare Parts. 5) Depreciation of Fixed Assets 8500,000 8500,000 8300297 (199,703) (F) (235) Over provision 6) Transportation, Communication Utility & Other Services 13228,000 13228,000 15,746,117 2518.117(A) 19.04 Due to increase in Hectricity& Telephone charges 7) Pensions, Retirement benefits 3,445,000 3,445,000 3312,759 (132241)(F) (3.84) Vacancies not filled 8) Gratuity Provision 900,000 900,000 4,827,854 3,927,854 (A) 436.43 Salary increase not budgeted 9) Losses & Writeoffs 25,000 25,000 2 9 3 4238(A) 16.95 Under Provision 10) Contribution, Grants and Subsidies 1,150,000 1,150,000 1,431,020 281,020 (A) 24.44 Due to increase in price of Tea supplied to staff. 11) Cultivations and filed Trials 3,715,000 3,715,000 4328305 613305(A) 1651 Increase in daily rates of Labour 12) Miscellaneous 7220,000 12,000 7232,000 4,196538 (3,035,462) (F) (4204) Less Overseas Training 273 Major Variations against the Budget Class of Item Budget Supplemen- Total Actual (A) Adverse Percentage Reasons given by Rs. tary Budget Rs. Rs. (Fj Favourable % the Management Rs. Rs. (A) Revenue a) Sale of GreenLeaf 2,420,000 2,420,000 3511,159 1,091,159(F) 45.09 Increase in price of Green Leaf b) Miscellaneous 1,827,000 01,827,000 4,279304 2.452304(F) 134.23 Increase in number of analytical samples and Disposal of Fixed (c) Capital Expenditure 1) Buildings 2274,300 325,000 2599300 33,046 (2566254) (F) (11284) Revoted far 1998 2) Plant Machinery & Lab Equiment 2577,000 60,000 2,637,000 2,448371 3) Office Equipment 4) Furniture Fittings and Household Equipment 5) Road, Water Supply, Electricity & Telephone (188,629) (F) (732) Revoted for 1998 1,781,100 778,000 2^ 59,100 2392,672 (166,428) (A) (934) Replace­ ment of 755,100 130,000 885,100 879,101 Computers (5,999) (A) (0.79) Increase in price of items 595,000 125,000 720,000 536,133 (193,867) (F) (3258) Works not 6) Motor Vehicle and bicycles 3,000,000 7) Workshop tools and Equipment 76500 3,000,000 3,645,000 76500 66,970 completed 645.000(A) 2150 Under estimated (9530) (F) (1246) Purchases not made 8) Other Fixed Assets 1,962,000 172,000 2134,000 1300341 (833,659) Annual board of survey reports on fixed assets and age analysis of debtors and creditors were not furnished for audit. 276 (c) (i) Department of Inland S t Coombs Estate Revenue Circular dated 24 August 1989 Turnover tax collected amounting to Rs. 60,669 to be remitted to the Department of Inland Revenue within 7 days had not been remitted upto the date of audit inspection. Rs. T R I - H . 0 36,265 1997 May - August St. Coomb's Estate22,367 1994 St. Joachim Estae 2,037 1995 60,669 (ii) Department of Inland Revenue Stamp Duty Act No. 43 in 1982 According to the Stamp duty Act No. 43 in 1982, the stamp duty amounted to Rs. 7,418 had not been remitted to the Inland Revenue Department as details given below. Amount Rs. (d) Establishment Code (i) Chapter xxiv Section 7.5 Passara sub station 1,166 from 1996 Deniyaya sub station 4,498 from 1995 - d o - 1,754 from 1996 7,418 Certificate of registration, revenue licence, confirmation of payment, etc. had not been furnished within two weeks after obtaining the loan by the officers mentioned below to confirm the utilisation of vehicle loans. Name of the officer Car loans F. Y. M. Maharoof K. M. S. M. Kulathunga Motor Cycle loans S. P. Jinasena R. Nadaraj R. Jayaraj W. P. A. N. Jayasinghe V. Shanmuganathan P. B. Ekanayake H. Jayaweera U. A. Wickramasinghe Amount granted Rs. 60,000 39,000 22,500 20,700 22,500 22,500 22,500 22,500 22,500 22,500 277 (ii) Chapter xxiv Section 2 (e) Ministry of Finance and Planning Circular No. MF/6/1/1/96 dated on 02 April 1996 Salary loans should be granted only for the purposes as specified under the Section xxiv of the Establish­ ment Code. Mrs. S. M. M. A. Jayakody had been granted an interest free special loan amounting to Rs. 94,398 in 1992, payable in 157 instalments over 13 years for her higher studies in U.K. Out standing balance as at 31 December 1997 was Rs. 57,121. Action had not been taken as yet to recover the loan eventhough this matter had been included in previous year audit report also. Advance amounting to Rs. 152,791 obtained by a Deputy Director on foreign visits made to Kenya in October 1996 had not been settled until 30 August 1998. (f) Financial Regulations No. 109 (1) and the Action had not been taken as per the F. R. 109 (i) in connection with the write-off of losses valued at Rs. 156,507 at the TRB - Head Office. No Board approval had also been taken for the write-off as well. (g) Employee Provident Fund Act No. 15 in 1958 (i) E.P.F. Contributions deducted from holiday pay aggregating Rs. 141,930 of St. Coombs Estate in February 1997 had been remitted to the Central Bank on January 1998. (ii) E. P. F. Contributions for February 1997, amounting to Rs. 3,067, deducted from the staff of Passara Sub-station, had not been remitted to the Central Bank as at the date of Audit Inspection. 278 Format - 38 Systems and Controls Main Functional Areas Detailed aspect General comments Detailed comments (a) Cheque receipts Delay in depositing at the Bank Poor in operation It was observed that long delays had been experienced in banking of cheques as details given below. Date of receipt of the Cheque and Number Date deposited at the Bank Value of the Cheque Rs. (b) Purchasing advances 97.01.01 182229 97.01.01 101191 97.01.29 143154 96.11.25 631124 96.11.18 631121 97.1.15 97.1.15 97.02.11 97.01.07 97.01.07 It was observed in audit Poor in that an unacceptable operation practice was experienced in of system payment of advances at the Low-country Station at Ratnapura. Advances were paid under temporary vouchers certified by the fficer in charge of the station. They prepare a fresh voucher and entered in the cash book when settlement of the advance and subsequentiy the temporary voucher is destroyed. Each and every advance shoud be entered into the cash book when the advance payment is made. 20,000.00 25.025.70 1,250.00 1,5000.00 1,000.00 The usual practice to enter the advance payment in the cash book when the advance payment is effected. It is entered in the cash book at the time of settlement. 279 (c) Bank Reconciliation Non-presented cheque Name Sri Lanka Telecom L. M. Lional TRI Sports Club T. Indrani (d) Festival and Action had not been taken Other to recover or write off the Advances balances of festival advance remained outstanding during last four years. - do - Non-presented cheques for more than six month period valued at Rs. 7,581 had not been taken in to the income of St. joachim Estate Ratnapura as details given below. Year Amount 25.00 2,934.93 980.00 3,641.27 7,581.20 Poor operation in system April 96 June 96 March 97 - d o - Actions had not been taken to recover or write off the following balance of festival advance given to the officers of St. Joachim estate and St. Coomb's estate. St Joachim Estate Year Amount Rs. 93/94 9,132.19 94/95 980.00 95/96 2,080.00 96/97 1,025.73 13,217.92 (e) Stocks (i) Auto diesel and Fire-wood-St. Coombs Estate St Coombs Estate M.P.C.S. - Nuwareliya 20,000 V. Sundar 8,445 do - There was no proper control over the stocks of St. Coombs Estate at Talawakele. It was revealed the following shortages and excesses at the audit verifications. Diesel (litre) Stock balance as at 1998.09.12 13,004 Physical verified balance 14,271 Excess/shortage 1267 Fire wood (CM.) 146 127.6 18.4 280 (f) Cheque receipt No follow up action had been taken in respect of unrealized cheques issued members. Poor operation in system (g) Cash advance payment Un-authorised cash Deviation of advance payment the system A cheque amounting to Rs. 26,594.02 - received on9.12.96fromMr.W.M. Premaratna of St. Coombs Estate, on account of settlement of distress loan balance had not been realized as at September 1998. No. follow up action had been taken in this connection. A cash advance of Rs. 9,750 had been paid to an office peon with out requisite authority and necessity. Amount Rs. 1740 Muthukumar 9,750 (h) Advances Long delay in settlement of advances Poor operation of control (i) Fuel stock Non-availability of measure - d o - (j) Tea Stock Non-use of bins do- Long delays were observed in setteng advances, granted to outside parties as well as employees for the purchases of goods and obtain services. The details of advances granted and delays observed are shown in Schedule I. The fuel stock could not be verified due to non­ availability of measure (dip - stick) which is a n essential part of the Cylinder tank located at St. Joachim Estate. It was observed in audit that made tea had been heaped up on the floor of the St. Joachim factory without using tea bins before packing. This situation leads to high moisture content and contamination of the made tea. 281 Schedule - Delays in settling advance Payment Settlement Balance Refund Outstan­ ding Name Date Amount Date Amount Date Amount Rs. Rs. Rs. Rs. Insurance Corp. 14.1.97 93,584 22,10.97 93,584 - G.P. Udumulla 27.1.97 800 14.2.97 - - Kandy offset printers 10.2.97 5,100 14.7.97 Associated newspapers Ceylon Ltd. 7.3.97 1,275 17.5.97 - - Brown & Co. Ltd. 24.3.97 4,000 15.5.97 - - Lake House Book Shop 7.3.97 5,616 31.12.97 - - B. D. G. Premadasa 31.3.97 1,000 16.6.97 - - Swedlanka Industries 11.4.97 53,493 18.11.97 - - D. K. W. Plastic Industries 5.6.97 28,386 01.10.97 - - Seelanatha Kuruppu & Sons Ltd. 6.5.97 37,375 17.9.97 - - Contractor Supplies (Management) Ltd. 4.6.97 3,650 23.9.97 - - Hayleys Engineering Ltd. 10.12.96 1,238,118 7.8.97 - - -do- 10.6.97 1,000,000 East West Information Systems Ltd. 16.7.96 181,850 8.8.97 - - Neshanya(Pvt.)Ltd. 25.3.95 47.500 27.5.97 - - St. Anthoney's Industries Group (Pvt) Ltd. 27.6.97 21,940 25.8.97 - - Brown & Co. Ltd. 30.12.96 3,000 12.6.97 - - B. Tilakarathna 8.8.97 15,000 6.12.97 28.11.97 5,975 - Ratnayake Rubber Industries 10.9.97 79,400 11,12.97 - - Diesel & Motor Eng. Co. Ltd. 23.9.97 132,590 18.11.97 - 14,280 12,250 Lake House Book Shop 22.2.96 5,616 31.12.97 - - Tune C/o Bank of Ceylon 20.6.94 1,872 31.12.97 - - 2,973,415 282 Format - 39 283 Vehicle Utilisation Certain significant information relating to the performance of the Board's vehicle fleet is given below. Petrol Diesel 1997 1996 1997 1996 Total Number of Vehicles as at 31 December Motor Cars (include cab vehicle) 04 - 11 Jeep - - 15 - Buses - - 04 - Vans - - 02 - Lorries 01 - 07 - MotorCycles 23 - - - Tractors - - 02 - Land Vehicles: Tractors - - 01 - Bulldozers - - - - Total = 2 8 = - 42 1997 1996 1997 1996 Type of Vehicle Type of Vehicle Type of Vehicle Type of Vehicle A B C D A B C D A B C D A B C D Performance No. of vehicles utilised during the year (excluding unserviceable 04 01 23 - - - - - 28 11 - 03 - - - - Distance travelled operated Km. 29,930 1531 74,637 1,029220 154,110 - 4,788 - - - - Fuel consumed in litres 3543 386 3,152 117,853 38256 - 3,882 - - - - Total expenditure on fuel Rs. 158,906 19,404 161,621 1,602,646 517,989 - 52430 - - - - Cost of repairs and maintenance (including insurance licence) Rs. 479,860 76,684 100277 4,496,120 1,882225 - 105256 - - - - Drivers' salaries, Bata and overtimeRs. Total cost (excluding drivers' remuneration) Rs. 638,766 96,088 261,898 6,098,766 2400214 - 157,686 - - - - ** Vide classification below. "Transport Fleet had not been submitted for 1996. 1997 1996 1997 1996 Type of Vehicle Type of Vehicle Type of Vehicle Type of Vehicle A B C D A B C D A B C D A B C D Performance per Kilometre/Hour* Average performance per litre in Km/in hours* 8.44 3.97 23.68 - - - - 8.73 4.03 - 123 - - - - Average expenditure per Km/hour* on fuel Rs. 5.31 12.67 116 1.56 3.36 - 10.95 - - - - Average expenditure per knVhour* on repairs andmaintenanoeRs. 16,03 50,09 1.34 4.37 1221 - 21.98 - - - - Average expenditure per km/hour* on drivers' remuneration Rs. Average cost per Km/hour*in respect of depredation Rs. Overall average expenditure per Km/hour Rs. 2134 62.76 351 5.92 1557 - 32.93 - - - - ** Classification Type of Vehicle Basis of Computation A. Passenger vehicles (Excluding buses) Kilometre B. Buses and Lorries -do - C. Motor Cycles - do - D. Land Vehicles * Hour J. D. R. Jayalath Assistant Auditor General for Auditor General 284 s^nufiDdb e^OFJLFIOA) CM j^riiif [blsnsiuii T E A R E S E A R C H I N S T I T U T E O F S R I L A N K A 5g<§ gOsf: <5ex50, sxstXKajetf Telegrams: 'RESEARCH' Talawakeile Sdjsasxs: ^ Nu. E. 052-8385 TELEPHONE: I Hatton 0512-601 REFERENCE No. CA/42A Yr. Ref: PI/H/TRB/96/163 QffOTji) auierb ST. COOMBS TALAWAKELLE, SRI LANKA 9th March 1998 The Auditor or General Auditor General's Department Independence Square, Colombo 7. Attention : Mr. J. D. R. Jayalath Assistant Auditor General Dear Sir, Report of the Auditor General on the accounts of the Tea Research Board for the year ended 31 December 1996 in terms of Section 14 (2) (c) of the Finance Act No. 38 of 1971 We refer to your letter dated 25th February 1998 on the above subject and for­ ward herewith our comments on the draft report. It is very much appreciated if you could review the report after taking into con­ sideration our comments dated 05th February 1997. Yours faithfully, Dr. S. D. I. E. Gunawardene Chairman Tea Research Board 9.3.98 W. B. Herath Senior Accountant TRI 93.98 INDEX 289 - 299 - Comments submitted on 5th February, 1998 300 - 303 - Director's and Deputy Director Research's observations 304 - Additional commentsfor draft report dated 25th February, 1998 COMMENTS ON THE AUDIT REPORT IN TERMS OF SECTION 13 (7) (a) OF THE FINANCE ACT NO. 38 OF 1971 ON THE ACCOUNTS OF TEA RESEARCH INSTITUTE FOR THE YEAR ENDED 31ST DECEMBER, 1996 Format 9 - Accounting Deficiencies a) Fixed Assets Description of the assets a) Nebulizer and rings purchased for Analytical Division b) Installation of monark burner for the factory at St. Joachim c) Cost of UPS system for St. Joachim Estate d) Jem motor for Water Pump e) Construction of retaining wall at St. Coombs Estate b) Fixed Assets c) Fixed Assets Depreciation d) Fixed Assets e) Fixed Assets f) Prior year adjustments Errors in capital and revenue allocation Auditors comment is incorrect. These spare parts were purchased for repairs. This has been adjusted in 1997 accounts This has been adjusted in 1997 accounts Auditors comment is incorrect. Jem motor for Water Pump had been correctiy charged to capital account in 1996. This has been adjusted in 1997 accounts Omission The goods received note for the Pressure Plate Apparatus Accessaries had been sent to CARP and was not available to us at the time of preparing the final accounts. Understatements/overstatements This has been adjusted in 1997 accounts This has been adjusted in 1997 accounts Errors in capital and revenue allocation These are capital nature of expenditure and had been correctly charged to capital accounts. Inappropriate disclosure We have been debiting the prior year adjustment entries as per SLAS 10 up to end of 1994. But Auditors in their report for the year ended 1994 highlighted that the expenditure of the prior year be charged to prior year adjustment account as shown in the following extracts of Auditors report 1994. 289 g) Fixed Assets h) Sales Overstatement i) Grants Received j) Foreign travelling advance Expenditure on telephone charges for 1993 amounting to Rs.. 2006.78 had been accounted for as expenditure for the year instead of being debited to prior expenses account. Expenditure on maintenance of colour separator during 1993 amounting to Rs. 8,000 had been accounted for as Expenditure for the year instead of being debited prior period items (PI/H/TRI/94/ 121-07.08.95) E. P. F. payable during 1993 amounting to Rs. 8,609.39 had been debited to labour wages instead of being debited to prior period items. Expenditure on railway bills for 1993 amounting to Rs. 7465.50 had been debited to personnel emoluments instead of being debited to prior items. In future the expenditure pertinent to prior year will be dealt as per SLAS 10. Inappropriate disclosure Fixed Assets in question are still the property of the Tea Research Board although they are located at the Sri Lanka Tea Board. This was the practice adopted by us all these years. However action will be taken to value the stock at the year end at COP or Net realizable value whichever is lower. Classification error Audit comment is correct. However this has not affected the final accounts presented for the year 1996. The ledger has been corrected in 1997. Classification error Amount advanced to Dr. G. D. Wimaladasa equivalent to Rs. 152,791/= was treated as a debt due from him as he has failed to submit a proper statement of expenditure. This classification error appearing in 1996 accounts was rectified in the books of accounts in the year 1997. 290 k) Insurance claim due 1) Fixed Assets m) Bonds outstanding n) Sub contractors St Coombs Estate Omission The amount stated in the Audit Report was not available to us at the time of preparing the final accounts. This has been accounted for in 1997 accounts. Omission Internal Auditor's report was not available to us at the time of preparing the final accounts. Adjusted in 1997 accounts. Omission These have been taken in to 1997 accounts. Legal action is contemplated against the officers through the Attorney General. Calculation error There was no overpayment. The credits for work completed had not been accounted. Accounts had been adjusted in 1997 accordingly. No double payment have been made. Format 10 - Verification of Physical Assets Class of Assets: TRB - Head Office (1) Stationery Stocks Shortages Excesses Rs. 228 Rs. 674 (3) Scientific Stocks Rs. 37,982 Rs. 9,576 (4) Drugs Stock (6) General Stocks Rs. 8,596Rs. 15,975 (7) Stocks at Ratnapura Rs. 750 Rs. 242 Format 11 - Confirmation of Assets Action will be taken to investigate and adjust the accounts in 1997. Action will be taken to investigate and adjust the accounts in 1997. Verification schedules had been submitted. Evaporation of petrol for the last 10 years. Action will be taken to investigate and adjust the accounts in 1997. In the letter by which a confirmation is invited, we have explicitly stated that in the absence of response, it will be concluded that the balance indicated is correct. 291 In respect of pre payments and advance payments we do not invite the confirmations since the documents from the payees are available which would confirm the correctness of the amounts. Format 12 - Confirmation of Liabilities Same comment as in the 1st para under Format 11 above. Format 13 - Unreconciled Control Accounts : This has been reconciled. (1) Fixed Assets (2) Total Expenditure under solar energy power (3) General Stock (4) Electrical Stock (5) Sundry Creditors (6) Labour Loans Kottawa (7) Labour Loans Passara St Coombs Estate (1) Festival Advance (2) Check Roll Debtors St Joachim Estate (1) Festival Advance Format 14 - Suspense Accounts (1) Suspense Accounts WO. Loss of Stationery Items Missing Table Loss of Petty Cash Amount payable to Ex-officer Mr. V. Selladurey This has been reconciled. This has been reconciled. This has been reconciled. This had been reconciled and detailed schedules had been submitted. This has been reconciled. This has been reconciled. This had been reconciled and the detailed schedules had been submitted. This had been reconciled and the detailed schedules had been submitted. The difference is the omission of the festival advance balance of Mr. Kamal Perera from the detailed schedule. Officer responsible had been dismissed from the services of the Institute L. T. Case is pending. This has been adjusted in 1997 accounts Officer responsible had been dismissed from the services of the Institute. L. T. Case is pending. Action will be taken to refund the balance after recovering for the missing items. (2) Suspense Account St. Coombs Estate Unidentified payments by sundry creditors Action will be taken to clear this account in 1997. 292 Format 15 - Lack of Documentary evidence for audit a) Vehicle running expenses b) Stocks Stocks at Estates St. Coombs Estate Rice and foodstuff General Stock Nursery Stock St. Joachim Estate Consumable Stock Nursery Stock c) Debtors St. Coombs Estate Sundry Debtors d) Deposit, Pre-payments, Advance for Purchases St. Coombs Estate Sub contractores e) Advances St. Coombs Estate Special Loan Labour Advance f) Creditors Provisions & Other Credit Balances St. Coombs Estate Sundry Creditors Detailed schedule will be forwarded. This has been sent subsequendy. This has been sent subsequendy. This has been sent subsequendy. This has been sent subsequently. This has been sent subsequendy. This has been sent subsequendy. The detailed schedule will be forwarded. The detailed Schedule will be forwarded. The detailed schedule will be forwarded. Attendance Bonus Format 16 - Age analysis of Debots a) Sundry Debtors The detailed schedule has been submitted. The detailed schedule will be forwarded. Mr. S. Sundaralingam former OIC, TRI Pelagahatenna has to perfect documents and forward same to REPPIA for the reimbursement of Rs. 96,840/-. The other amounts are dues from ex-TRI officers. In respect of these there are L.T. cases pending and once the outcome is known these will be recovered from the amounts due for payments. 293 b) Recovery Account c) Trade Debtors (d) Insurance Claims Due e) Bonds Outstanding : h) Analytical Charges Due St. Coombs Estate St. Joachim Format 17 - Age Analysis of Creditors TRB - Head Office b) Tender Deposit Refundable Sundry Creditors St. Coombs Estate St. Joachim Estate Format 19 - Identified Losses a-f) Loss of Petty Cash, Salary Packets, Medical Claims Travelling Claim, etc.: g) Loss of items from Electrical Foreman's Bungalow Rs. 1,100/= due from ex-TRI officers cannot be recovered seeking approval for write-off. Rs. 1000/= due from Hopton Group for supplying cuttings from Passara TRI Centre. OIC Passara will follow action to recover the amount. Period of claims had been lapsed. Action will be taken to investigate and seeking approval for write-off. Files have been handed over to Attorney General Dept. for necessary Legal action. Attempts to recover analytical service charges from Private small holders have failed. Seeking approval for a write-off. Action will be taken by the new Superintendent for the recoveries. Action will be taken by the new Superintendent for the recoveries. Action will be taken to credit the TRB Miscellaneous Income account if not claimed. Action will be taken to credit the TRB Miscellaneous Income account if not claimed. Action will be taken to credit the TRB Miscellaneous Income account if not claimed. The officer responsible had been dismissed from the services of the institute after a domestic inquiry. Labour Tribunal case is pending. The cost of items lost was recovered from the former Electrical Foreman, but had to be refunded on Assistant Commissioner of Labour's directive. Seeking approval for a write-off. 294 h) Ban Charges i-j) Missing "Mettler" balance at Plant Physicology Division and loss of lab equipments investigated by the internal auditors All the expenditure of L/C and other charges had been debited to Research Units. This is being investigated by the Lindula Police. Format 25-Other Slow-^noving Idle and under-utilized Assets and Idle labour Estate Bungalow St. Joachim Estate Electrical Stock and General Stock - HO Dismantled items at St. Joachim Tea Factory at Lameliere Division St. Coombs Estate Vehicles a) Reimbursement of Medical Claim b) Patent Rights c) Gasifier and steam boner/Radiator : system for withering and during project of Ratnapura d) Foreign Scholarships e) Workers' Housing Electrification L. T. Case is pending against the Factory officer who had been interdicted. Action will be taken to gain possession of the house and household items. These are slow moving items and will be used whenever required. Action will be taken to dispose the items by an Auction. The Green Tea Project would not be re-activated until the staff in the Technology Division is trained. Action will be taken to dispose the items by an Auction. Format 26 - Un economic Transaction Actions per Medical Fund Rules, 50% of the Medical Claim could be reimbursed for the officer and his/her family. A party who is interested had already applied for a BOI project to produce this product in commercial scale and negotiations/discussions are currently underway to finalize the transfer of the patent rights. M/S Colombo Commercial Co. Ltd. failed to deliver the goods under the contract. This matter has been brought to the notice of the Attorney General's Department to pursue legal action. Legal action contemplated against the officers through the Attorney General. Work has been completed in 1997. 295 f) Goods in transit b) Radopholus Projects c) Energy Efficiency Solar Energy Project d) Ortho-CTC Project Goods had already been cleared and the entries will be passed in 1997. Action will be taken to write-off. (l)However, at the end of the year the total factory weight is higher than the check roll weight. St. Coombs Estate was running without a permanent Superintendent for the last three years. Now a permanent Superintendent has been appointed the plucking standard would be improved. This is an on-going project. The expenditure of fertilizer and travelling had been inadvertently charged to B/FERT and D/AGCH. This is an on-going project and in progress This is an on-going project and is in the final stage. Action will be taken by the Institute's Tender Board as tender limits have been enhanced as per new guideline by Government on purchasing. Format 36 - Non compliance with Laws, Rules, Regulation and Management Decision Reference to Laws, Rules, Regulation etc. a) Finance Act (i) Section 8 (1) (ii) Section 13 (5) (b) The reason for delay was the resignation of the Senior Accountant in April 1996 and the new Senior Accountant was appointed on 16/12/1996. However, every attempt will be made to get the budget approved three months before the year end in future. Contents noted. Action will be taken to submit the reports of the stock and stores from the year 1997 onwards. 296 g) Insurance claims due Format 30 - Rates and Percentages a) According to the accepted estate : practices the check roll weight should be below than factory weight b) According to the accepted elevation averages, the refuse tea percentage on made tea should be 4% in Nuwara-Eliya area Format 33 - Delays in Projects Description of the Project a) Field trial on sul-po-mag. b) Financial Regulations F. R. 756 c) Establishment Code (i) Chapter XXTV Section 2 (ii) Chapter XXTV Section 7.5 d) Department of Inaland Revenue Circular dated 24 August 1989 e) Ministry of Finance and Planning Circular No. MF/6/1/1/96 dated 2 April 1996 f) Tea Research Board Circular SO/7/91 dated 13 December 1991 Format 38 - Systems and Controls Petty Cash Authorization of Journal Vouchers Encashment of Personnel cheques Assets were verified continuously from 1994 to mid 1996. Hence, an annual verification was not carried out in 1996. However, from 1997 action will be taken to verify assets at the end of the year. This had been inadvertendy debited to loan account instead of debtors. This facility has been provided to Mrs. J.A.A.M. Jayakody to gain specialization in agriculture economics, a subject matter area a crucial importance in evaluating the new technologies developed by the Institute. Disciplinary action will be taken against the officers who had failed to produce the certificate of registration, revenue license, etc. for the vehicle loans obtained. Action will be taken to remit the turnover tax collected to the Inland Revenue Department early. Amount advanced transferred to Debtors Account. Action will be taken to recover the dues early. Ministry of Plantation Industries had called quotations for the purchase of vehicles to the Ministry and using the same quotations/ tenders, with the approval of the Secretary of MPI, the Institute purchased vehicles considering the exigency/urgency of vehicles. TRI Sports Club Petty Cash had been taken into TRB Petty Cash. These Journal entries had now been approved. General Stock and Electrical Stock issue vouchers were fed into the Computer and the sorted print outs were treated as Journal Vouchers. These vouchers were approved by an Accountant. Officers of the Board are entitled to encash two personal cheques upto Rs. 2,500/- per month. If the cheque is returned by the Bank with the remark "Refer to drawer" the facility enjoyed by the officer would be suspended for one year. 297 Fixed Assets Register Advance Salaries Fuel Pump Surety bonds for Cashiers Register of trees Control on Transport Section Check roll wages Check roll wages Action has been taken to computerize the Fixed Assets Register. Interest had been charged at the rate of 30% per annum for the delay in settling the advances taken by the officers. Delay was due to non receipt of invoices and goods received notes in time. Salary advances are paid to officers within the permitted limits. The negative balances appearing in the Pay lists are due to recoveries made other than the statutory deductions. Action had been taken to restrict other recoveries from the pay sheets. The underground tank is tilted and accurate balance cannot be ascertained as a result. The Post of Cashier is vacant and is being attended temporarily by a senior officer of the Institute. Action will be taken to maintain registers at the Estates for valuable trees. (i) The Log Book of vehicle No. 14/8958 had been submitted subsequently. Vehicle No. 14/5011 had been lent to TSHDA temporarily. Log Book of vehicle No. 14/4675 is being maintained. (ii) These had been corrected. (iii) These vehicles had to be used even postponing repairs due to scarcity of vehicles to meet Institute's commitment. It is not possible to obtain the signature/ thumb impression of the labourers when the payments are being made. The check roll cannot be released in advance to get the signature due to security reasons. The Labourers are not interested to sign after receiving the money, hence this is not practicable. Labourers mentioned in the report are working in the Estate. 298 Maternity Benefits Cash work Muster chits and pocket check roll abstracts Stores Control St. Coombs Estate Staff Officers overtime St. Coombs Estate Advance Salary payment for St. Coombs Estate Sub-contractors EPF Nos. of the following had been corrected. Sooriyakumarie - 2179 Thanalectumy -2182 Cash workers and seasonal workers are offered work during the cropping season. This is being investigated. Goods received notes are issued to all purchases. This is the practice in the Estate. In addition to the normal monthly advance, on exceptional circumstance, advances are paid to workers on a separate register and recovered in the same month. (i) Action has been taken to maintain a register for contracts. (ii) Action has been taken to reconcile. (iii) Contracts forms had been signed by the Contractors. (iv) Delay in obtaining raw materials. (v) There was no overpayment. (vi) Mr. I. D. Gunaratne was the Actg. Superintendent. 299 AUDITOR GENERAL'S REPORT, 1996 (pages 16 to 18) 3:2 Research Projects Significant Factors Identified (a) Important that yield/ha be increased to levels in other tea-producing countries. Response : Agreed. However, yield increases depend on many factors not entirely in the control of the Tea Research Board (TRB). The Tea Research Institute (TRI) endeavours to produce higher yielding planting material, and advises constantly on soil preservation and good agricultural practices. (b) Poor calibre of personnel in all segments of tea sector resulting in inefficiencies in production. Response : Agreed. This is sensu strictu outside the influence of the TRB. However, the TRI is collaborating actively, in Asian Development Bank (ADB) - funded programmes, with the Ministry of Plantation Industry, the Planters' Association (PA), the Tea Small Holders Development Authority (TSHDA) and the National Institute of Plantation Management in organising and mounting training programmes at all levels and in advising on recruitment procedures. (c) Failure of the TRB in assisting policy formulation based on scientific grounds. Response : The Chairman of the TRB, and the Director and Deputy Directors Research of the TRI, are in point of fact members of the Tea Sector Review which meets every month under the chairmanship of the Secretary, Ministry of Plantation Industries. The purpose of their membership is to advise and assist in policy formulation based on scientific grounds, and this is being done as the Minutes and the decisions taken at these meetings will show. Researches not serving the pressing needs on the end users. Response : While arguably this was the case even in the period covered by the 1996 Report, in recent months there have been more positive statements and a growing appreciation of the new directions the TRI has embarked on, from the PA, the TSHDA, company executives and other elements and individuals in the tea industry. These new directions, initiated over the last several months, are described in the 1998-2002 Corporate Plan of the TRI. The TRI now has a modern outlook with a well-defined corporate Mission and goals. An independent opinion on the TRI's recent record in serving one important sector of the end users, namely the smallholders, is contained in the Progress Report on the First Phase of the Smallholder Tea Development Project issued in December 1997 by an evaluation team from the Asian Development Bank. The Report recognises that the TRI is active both in smallholder extension and in new research and advisory programmes designed specifically for smallholders. The Report recognises also the usefulness of the recent extension, by the TRI, of the Experiments and Extension (E & E) Forum concept, hitherto restricted to the plantation sector, to participants from the smallholder sector. (The first TRI smallholder E & E was held on 5 December 1997 and was the first E & E ever to be held in Sinhala.) 300 (d) Lack of a long-term energy policy for the tea processing sector. Insufficient research on hydro power, solar energy and energy conservation. (e) Inter-/multi-cropping of tea with rubber and other crops not popularised. Response: Popularisation depends on a complex of factors outside the influence of the TRB. However, data generated by the TRI are in fact available for inter cropping tea with rubber, coconut and export agricultural crops, in order to increase the profitability of marginal land. For instance, guidelines have been issued for intercropping tea with rubber. Similarly, guidelines have been prepared for intercropping tea with coconut, and with export crops such as coffee and pepper. (f) Lack of a mechanical plucking system Response : This is not true anymore. The TRI patented a manual shear in 1997. It is light and manoeuverable and allows for faster harvesting as well as leaf selectivity which serves to ensure quality of the product. The TRI has given the manual shears to estates and smallholders, at their request, for testing. They report excellent results and performance data are now being examined. A Sri Lankan company has mass -produced these shears and they are available for sale to producers. The use of shears will greatly increase worker productivity, and reduce the worker requirement for harvesting by about one half. (g) Lack of efficient engineering services to the plantations. Observations on Actions proposed by the Board (i) Development of high-yielding, elevation-specific clones. Response : Evaluations have in fact been done on the TRI 3000 clonal series released in 1976 and the TRI 4000 series released in 1990. Depending on soil and climate, the TRI 3000 series may yield 2,500 - 3,200 kg mt/ha/year, and combine yield potential with drought, and pest and disease, tolerance. The TRI 4000 series may yield 4,000 kg mt/ha/year and is more drought-tolerant. Thirty selections of the TRI 5000 series are presendy being evaluated and are giving yields of 3,000 - 5,000 kg mt/ha/year. Overall national yields from clones are now at about 2,500 kg mt/ha/year at high elevations and considerably more at lower elevations, compared to less than 1,000 kgmt/ha/year from seedling tea. Diagnostic survey on fertiliser application. Response : Data from the questionnaires are presentiy being processed and evaluated by the appropriate scientists. Mass production of clones to meet requests from estates and small holders. Response: It is now generally recognised in the tea sector, and presumably also by the ADB, that the TRI cannot possibly participate in mass production of any sort because this will result in moving limited staff and facilities away from research and extension. These are TRI's primary concern. Production of planting material in the huge quantities required to satisfy the Sri Lankan requirements (40 to 95 million plants per annum) is best done on a commercial scale by 301 smallholder societies. Interested estates and entrepreneurs. All the TRI can do is to assist in selection of sites, registration, supply of cuttings, and periodic inspections of the commercial nurseries and mother bush areas set up. (ii) CADMAR shortfall. Response : The CADMAR process will be completed in April/May 1998. The research thrusts and projects were formulated at a series of multi-disciplinary in-house meetings of TRI scientists, and the scientists themselves, under the chairmanship of the Director, took the process through the required steps. This is the preferred approach. The CADMAR formulation and prioritisation do not need the direct involvement of the TRB and its Consultative Committee on Research. (iii) Technology trials and experiments Due to heavy crop during the period under review, the high capacity drier FBD- 4 was operated. The FBD-3 which is coupled with the Economizer (at St. Coombs Estate) is a low capacity drier and was not operated. Therefore the trials on the Economizer could not be carried out. Two types of Gasifiers have been installed at St. Joachim Estate. 1. BECE-KARA This gasifier was used for electricity generation on and off especially during the days of power crisis. 2. NERD gasifer This gasifier could not be operated due to poor design. NERD engineers could not repair it and put it in order. (iv) Additional research projects Response: Approximately 80 applied research projects have been formulated to date in the CADMAR process, in order to address the problems identified both at the 1995 workshops and in later interactions within and outside the TRI. In addition, a long list of basic projects are presently being prioritised for the purpose of understanding and diagnosing the applied problems. (v) Progress in tissue culture Response: Already the TRI has developed a protocol for the tissue culture of tea and lodged an application for a patent. A Memorandum of Understanding has been entered into with a Sri Lankan company for using the growth and rooting media detailed in the patent protocol for the mass production of plandets. Suspension of research on green tea Response : Research into green tea has been suspended, mainly owing to the excellent prices being obtained for black tea for which Sri Lanka is well known. The present needs of end users do not include research on green tea. It is expected that the good market for black tea will persist into the foreseeable future. 302 No development of machinery for black tea manufacture owing to lack of staff. Response : Agreed. The TRI has lost experienced and qualified staff over the last several years owning to the poor conditions of employment. Overtures are being made to the authorities by the TRI for the revision of terms and conditions, with a view to recruiting and retaining good quality staff in the TRI. WWDM 8 March 1998 303 Comments on the Draft Report of the Auditor General on the accounts of the Tea Research Board for the year ended 31December 1996 in terms of Section 14 (2) (c) of the Finance Act No. 38 of 1971 1.3. Provision in the Tea Research Board Act No. 52 of 1993 a) Section 15 (5) of the Act 1) The TRB has initiated action to get the Act amended and the Hon. Minister has given his concurrence to be forwarded to the Parliament. 2.5. Comments on Accounts 2.5.1. Accounting Policies b) The expenditure had been disclosed under different headings. Action will be taken to disclose the expenditure under respective categories according to SLAS No. 10. 2.5.2. Accounting Deficiencies h) An advance amounting to Rs. 152,791 made to an officer in connection with the foreign visit to Kenya had been treated as a debt due from him as he had failed to submit a proper statement of expenditure. 3.2. Research Projects Report from Director, TRI is sent herewith. 3.8. Uneconomic Transactions a) The TRB has decided an upper limit of Rs. 250,000/- for reimbursement of Medical Claims which will be intimated to the Trustees of Medical Fund in order for implementation. 3.12. Vehicle Utilization a) The log book of «vehicle No. 14/8958 had been handed over to the Superintendent of Government Audit subsequendy. 304